Restriction map for
pFN21AE5227
(Circular) (Six-base) MAPSORT of: pFN21AE5227.seq Check: 9653 from: 1 to: 5287
pFN21AE5227 5287 bp
With 182 enzymes: SgfI *
January 30, 2014 17:15 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3097 278
Size: 53 83 186 936 1561 2468
Fragments arranged by size:
2468 1561 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2630 1937
Size: 693 4594
Acc65I G'GTAC_C
Cuts at: 2608 2608
Size: 5287
AclI AA'CG_TT
Cuts at: 1563 3414 3787 1563
Size: 1851 373 3063
Fragments arranged by size:
3063 1851 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2316 3350 4734 5002 1632
Size: 684 1034 1384 268 1917
Fragments arranged by size:
1917 1384 1034 684 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5090
AflIII A'CryG_T
Cuts at: 1203 4186 5163 1203
Size: 2983 977 1327
Fragments arranged by size:
2983 1327 977
AgeI A'CCGG_T
Cuts at: 4118 4118
Size: 5287
AhdI GACnn_n'nnGTC
Cuts at: 4016 4016
Size: 5287
AleI CACnn'nnGTG
Cuts at: 1166 2108 1166
Size: 942 4345
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5287
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2513 2545 2513
Size: 32 5255
AlwNI CAG_nnn'CTG
Cuts at: 2314 2439 3030 4602 2314
Size: 125 591 1572 2999
Fragments arranged by size:
2999 1572 591 125
ApaI G_GGCC'C
Cuts at: 2205 2548 2205
Size: 343 4944
ApaLI G'TGCA_C
Cuts at: 3344 4500 3344
Size: 1156 4131
ApoI r'AATT_y
Cuts at: 1493 2596 2822 2974 5083 1493
Size: 1103 226 152 2109 1697
Fragments arranged by size:
2109 1697 1103 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3841 160
Size: 872 2809 1606
Fragments arranged by size:
2809 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5287
AvaI C'yCGr_G
Cuts at: 1870 1943 2413 2612 1870
Size: 73 470 199 4545
Fragments arranged by size:
4545 470 199 73
BamHI G'GATC_C
Cuts at: 2617 2984 2617
Size: 367 4920
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2428 2608 4063 618
Size: 325 217 627 641 180 1455 1842
Fragments arranged by size:
1842 1455 641 627 325 217 180
BanII G_rGCy'C
Cuts at: 1391 2205 2253 2548 2606 1391
Size: 814 48 295 58 4072
Fragments arranged by size:
4072 814 295 58 48
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4316
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3478 3512 1903
Size: 34 1541 34 3678
Fragments arranged by size:
3678 1541 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3179 4389 3179
Size: 1210 4077
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5287
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3898 136
Size: 107 122 71 631 605 337 1889 1525
Fragments arranged by size:
1889 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5282 5282
Size: 5287
BlpI GC'TnA_GC
Cuts at: 2704 2704
Size: 5287
Bme1580I G_kGCm'C
Cuts at: 1790 2205 2548 3348 4504 1790
Size: 415 343 800 1156 2573
Fragments arranged by size:
2573 1156 800 415 343
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5287
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3018 3976 442
Size: 416 53 83 399 24 1601 958 1753
Fragments arranged by size:
1753 1601 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5287
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2084 2300 2573 3947 1239
Size: 845 216 273 1374 2579
Fragments arranged by size:
2579 1374 845 273 216
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3412 4277 4575 3412
Size: 865 298 4124
Fragments arranged by size:
4124 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2306 3950 882
Size: 617 807 1644 2219
Fragments arranged by size:
2219 1644 807 617
BsaAI yAC'GTr
Cuts at: 493 1204 3078 493
Size: 711 1874 2702
Fragments arranged by size:
2702 1874 711
BsaBI GATnn'nnATC
Cuts at: 5281 5281
Size: 5287
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3094 3476 4111 275
Size: 53 83 186 936 1561 382 635 1451
Fragments arranged by size:
1561 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3720 4118 4392 4539 4950 3720
Size: 398 274 147 411 4057
Fragments arranged by size:
4057 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2513 2543 1178
Size: 30 165 30 1110 30 3922
Fragments arranged by size:
3922 1110 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2081 1186
Size: 895 4392
BseYI C'CCAG_C
Cuts at: 2454 4490 4986 2454
Size: 2036 496 2755
Fragments arranged by size:
2755 2036 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2335 1163
Size: 573 599 4115
Fragments arranged by size:
4115 599 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2737 3498 4526 1540
Size: 275 186 736 761 1028 2301
Fragments arranged by size:
2301 1028 761 736 275 186
BsiHKAI G_wGCw'C
Cuts at: 3348 3433 4504 3348
Size: 85 1071 4131
Fragments arranged by size:
4131 1071 85
BsiWI C'GTAC_G
Cuts at: 2493 2493
Size: 5287
BsmI GAATG_Cn'
Cuts at: 2798 2891 2798
Size: 93 5194
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2205 2253 2548 2606 3348 3433 4504
Size: 399 415 48 295 58 742 85 1071
Cuts at: 4504 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 415 399 295 85 58
48
BspEI T'CCGG_A
Cuts at: 4950 4950
Size: 5287
BspHI T'CATG_A
Cuts at: 3176 4906 3176
Size: 1730 3557
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2136 2642 844
Size: 1292 506 3489
Fragments arranged by size:
3489 1292 506
BsrBI CCG'CTC
Cuts at: 1394 1823 3174 1394
Size: 429 1351 3507
Fragments arranged by size:
3507 1351 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3782 3956 65
Size: 1183 2534 174 1396
Fragments arranged by size:
2534 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3931 4118 1700
Size: 110 2121 187 2869
Fragments arranged by size:
2869 2121 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5287
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5287
BssSI C'ACGA_G
Cuts at: 3347 4359 3347
Size: 1012 4275
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5287
BstBI TT'CG_AA
Cuts at: 4091 4091
Size: 5287
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5287
BstXI CCAn_nnnn'nTGG
Cuts at: 2062 2450 2062
Size: 388 4899
BstYI r'GATC_y
Cuts at: 1972 2617 2984 3369 3386 4838 4924 4936 5282
Size: 645 367 385 17 1452 86 12 346
Cuts at: 5282 1972
Size: 1977
Fragments arranged by size:
1977 1452 645 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2508 2508
Size: 5287
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3796
Fragments arranged by size:
3796 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2519 487
Size: 683 56 1293 3255
Fragments arranged by size:
3255 1293 683 56
BtsI GCAGTG_nn'
Cuts at: 2803 3597 3617 2803
Size: 794 20 4473
Fragments arranged by size:
4473 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5287
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5252
DraI TTT'AAA
Cuts at: 2591 2591
Size: 5287
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4294 809
Size: 507 2978 1802
Fragments arranged by size:
2978 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2316 2734 3623
Size: 54 1425 50 275 18 486 418 889
Cuts at: 3623 5252 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 486 418 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2734 1537
Size: 275 922 4090
Fragments arranged by size:
4090 922 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3217 1353
Size: 1864 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2528 3874 4248 4394 387
Size: 1064 138 939 1346 374 146 1280
Fragments arranged by size:
1346 1280 1064 939 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2300 2316 2573 3350 3947 4734
Size: 393 452 216 16 257 777 597 787
Cuts at: 4734 5002 1239
Size: 268 1524
Fragments arranged by size:
1524 787 777 597 452 393 268 257
216 16
EcoO109I rG'GnC_Cy
Cuts at: 2201 2374 2458 2545 2201
Size: 173 84 87 4943
Fragments arranged by size:
4943 173 87 84
EcoRI G'AATT_C
Cuts at: 2596 2974 2596
Size: 378 4909
FspI TGC'GCA
Cuts at: 3793 3793
Size: 5287
HaeII r_GCGC'y
Cuts at: 1678 4434 1678
Size: 2756 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2318 2350 3928 3960 4002
Size: 32 135 32 746 32 1578 32 42
Cuts at: 4002 4034 1373
Size: 32 2626
Fragments arranged by size:
2626 1578 746 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2631 2877 669
Size: 988 281 693 246 3079
Fragments arranged by size:
3079 988 693 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5287
HpaI GTT'AAC
Cuts at: 2877 2877
Size: 5287
KpnI G_GTAC'C
Cuts at: 2612 2612
Size: 5287
MfeI C'AATT_G
Cuts at: 2886 2886
Size: 5287
MluI A'CGCG_T
Cuts at: 5163 5163
Size: 5287
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4401 4585 1305
Size: 3096 184 2007
Fragments arranged by size:
3096 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3465
Fragments arranged by size:
3465 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2108 3245 3604 3763 518
Size: 648 942 1137 359 159 2042
Fragments arranged by size:
2042 1137 942 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2585 2703 3380 4528 4773 1606
Size: 979 118 677 1148 245 2120
Fragments arranged by size:
2120 1148 979 677 245 118
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3796
Fragments arranged by size:
3796 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5287
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5287
NotI GC'GGCC_GC
Cuts at: 2734 2734
Size: 5287
NspI r_CATG'y
Cuts at: 1132 1229 2645 4190 1132
Size: 97 1416 1545 2229
Fragments arranged by size:
2229 1545 1416 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5287
PciI A'CATG_T
Cuts at: 4186 4186
Size: 5287
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2314 1108
Size: 1206 4081
PmeI GTTT'AAAC
Cuts at: 2591 2591
Size: 5287
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2513 2545 3356 3388 4869 4901 2513
Size: 32 811 32 1481 32 2899
Fragments arranged by size:
2899 1481 811 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2374 2458 2374
Size: 84 5203
PshAI GACnn'nnGTC
Cuts at: 1613 2223 1613
Size: 610 4677
PsiI TTA'TAA
Cuts at: 2857 2857
Size: 5287
PspOMI G'GGCC_C
Cuts at: 2201 2544 2201
Size: 343 4944
PstI C_TGCA'G
Cuts at: 830 2562 2639 830
Size: 1732 77 3478
Fragments arranged by size:
3478 1732 77
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5287
PvuII CAG'CTG
Cuts at: 2585 2585
Size: 5287
SalI G'TCGA_C
Cuts at: 1936 2629 1936
Size: 693 4594
SanDI GG'GwC_CC
Cuts at: 2374 2458 2374
Size: 84 5203
SbfI CC_TGCA'GG
Cuts at: 2639 2639
Size: 5287
ScaI AGT'ACT
Cuts at: 2567 3535 5247 2567
Size: 968 1712 2607
Fragments arranged by size:
2607 1712 968
SfcI C'TryA_G
Cuts at: 826 1045 2558 2635 3770 4451 4642 826
Size: 219 1513 77 1135 681 191 1471
Fragments arranged by size:
1513 1471 1135 681 219 191 77
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5287
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5287
SmaI CCC'GGG
Cuts at: 1872 2614 1872
Size: 742 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3391 4292 4554 820
Size: 197 926 1448 901 262 1553
Fragments arranged by size:
1553 1448 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3078 493
Size: 2585 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5287
SphI G_CATG'C
Cuts at: 1132 2645 1132
Size: 1513 3774
SspI AAT'ATT
Cuts at: 5 52 3211 5
Size: 47 3159 2081
Fragments arranged by size:
3159 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5287
StyI C'CwwG_G
Cuts at: 513 1065 2004 2242 2726 513
Size: 552 939 238 484 3074
Fragments arranged by size:
3074 939 552 484 238
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2446 3314 3331 3484 3669 4114 325
Size: 1255 866 868 17 153 185 445 1498
Fragments arranged by size:
1498 1255 868 866 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2565 3533 5245
Size: 274 80 33 51 1443 588 968 1712
Cuts at: 5245 96
Size: 138
Fragments arranged by size:
1712 1443 968 588 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5247 5279 5247
Size: 32 5255
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5287
XbaI T'CTAG_A
Cuts at: 2623 2623
Size: 5287
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5287
XmaI C'CCGG_G
Cuts at: 1870 2612 1870
Size: 742 4545
XmnI GAAnn'nnTTC
Cuts at: 3416 3416
Size: 5287
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3095 276
Size: 53 83 186 936 1561 2468
Fragments arranged by size:
2468 1561 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI
SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI