(Linear) (Six-base) MAPSORT of: KIEE5227.seq Check: 7075 from: 1 to: 582 KIEE5227.orf With 182 enzymes: SgfI * January 29, 2014 11:51 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 311 582 Size: 311 271 AleI CACnn'nnGTG Cuts at: 0 103 582 Size: 103 479 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 508 540 582 Size: 508 32 42 Fragments arranged by size: 508 42 32 AlwNI CAG_nnn'CTG Cuts at: 0 309 434 582 Size: 309 125 148 Fragments arranged by size: 309 148 125 ApaI G_GGCC'C Cuts at: 0 200 543 582 Size: 200 343 39 Fragments arranged by size: 343 200 39 AvaI C'yCGr_G Cuts at: 0 408 582 Size: 408 174 BanI G'GyrC_C Cuts at: 0 423 582 Size: 423 159 BanII G_rGCy'C Cuts at: 0 200 248 543 582 Size: 200 48 295 39 Fragments arranged by size: 295 200 48 39 Bme1580I G_kGCm'C Cuts at: 0 200 543 582 Size: 200 343 39 Fragments arranged by size: 343 200 39 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 79 295 568 582 Size: 79 216 273 14 Fragments arranged by size: 273 216 79 14 BsaI GGTCTCn'nnnn_ Cuts at: 0 301 582 Size: 301 281 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 508 538 582 Size: 508 30 44 Fragments arranged by size: 508 44 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 76 582 Size: 76 506 BseYI C'CCAG_C Cuts at: 0 449 582 Size: 449 133 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 330 582 Size: 330 252 BsiWI C'GTAC_G Cuts at: 0 488 582 Size: 488 94 Bsp1286I G_dGCh'C Cuts at: 0 200 248 543 582 Size: 200 48 295 39 Fragments arranged by size: 295 200 48 39 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 131 582 Size: 131 451 BstXI CCAn_nnnn'nTGG Cuts at: 0 57 445 582 Size: 57 388 137 Fragments arranged by size: 388 137 57 Bsu36I CC'TnA_GG Cuts at: 0 503 582 Size: 503 79 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 514 582 Size: 514 68 EaeI y'GGCC_r Cuts at: 0 311 582 Size: 311 271 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 523 582 Size: 523 59 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 79 295 311 568 582 Size: 79 216 16 257 14 Fragments arranged by size: 257 216 79 16 14 EcoO109I rG'GnC_Cy Cuts at: 0 196 369 453 540 582 Size: 196 173 84 87 42 Fragments arranged by size: 196 173 87 84 42 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 313 345 582 Size: 313 32 237 Fragments arranged by size: 313 237 32 MslI CAynn'nnrTG Cuts at: 0 103 582 Size: 103 479 PflMI CCAn_nnn'nTGG Cuts at: 0 309 582 Size: 309 273 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 508 540 582 Size: 508 32 42 Fragments arranged by size: 508 42 32 PpuMI rG'GwC_Cy Cuts at: 0 369 453 582 Size: 369 84 129 Fragments arranged by size: 369 129 84 PshAI GACnn'nnGTC Cuts at: 0 218 582 Size: 218 364 PspOMI G'GGCC_C Cuts at: 0 196 539 582 Size: 196 343 43 Fragments arranged by size: 343 196 43 PstI C_TGCA'G Cuts at: 0 557 582 Size: 557 25 SanDI GG'GwC_CC Cuts at: 0 369 453 582 Size: 369 84 129 Fragments arranged by size: 369 129 84 ScaI AGT'ACT Cuts at: 0 562 582 Size: 562 20 SfcI C'TryA_G Cuts at: 0 553 582 Size: 553 29 StyI C'CwwG_G Cuts at: 0 237 582 Size: 237 345 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 441 582 Size: 441 141 TatI w'GTAC_w Cuts at: 0 560 582 Size: 560 22 Enzymes that do cut: AcuI AleI AloI AlwNI ApaI AvaI BanI BanII Bme1580I BpmI BsaI BsaXI BseRI BseYI BsgI BsiWI Bsp1286I BspMI BstXI Bsu36I BtgZI EaeI EciI Eco57MI EcoO109I Hin4I MslI PflMI PpiI PpuMI PshAI PspOMI PstI SanDI ScaI SfcI StyI TaqII TatI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsiEI BsiHKAI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtsI ClaI CspCI DraI DraIII DrdI EagI EarI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI