Restriction map for
pFN21AB3507
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BamHI BbeI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsoBI BspEI BspHI BsrBI BsrDI
BssHII BstAPI BstBI BstEII BstPI
BstUI BstYI CelII CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MamI
MfeI MflI MluI MroI MspA1I
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 893 GACGTC GACGT^C
AccI 408 GTATAC GT^[AC][GT]AC
AcyI 893 GACGTC G[GA]^CG[CT]C
AluI 284 AGCT AG^CT
AocI 61 CCTCAGG CC^T[ACGT]AGG
ApaI 466 GGGCCC GGGCC^C
BalI 987 TGGCCA TGG^CCA
BbrPI 1071 CACGTG CAC^GTG
BbvCI 8 CCTCAGC CCTCAGC(-5/-2)
BsaAI 1071 CACGTG [CT]AC^GT[GA]
BsaHI 893 GACGTC G[GA]^CG[CT]C
BsaI 328 GAGACC GGTCTC(1/5)
BsgI 746 GTGCAG GTGCAG(16/14)
BsmAI 328 GAGAC GTCTC(1/5)
BsmI 971 GCATTC GAATGC(1/-1)
Bsp1407I 47 TGTACA T^GTACA
BspDI 97 ATCGAT AT^CGAT
BspMI 42 GCAGGT ACCTGC(4/8)
BsrFI 1090 ACCGGC [GA]^CCGG[CT]
BsrGI 47 TGTACA T^GTACA
BssSI 235 CTCGTG CACGAG(-5/-1)
Bst1107I 408 GTATAC GTA^TAC
BstXI 152 CCAAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 408 GTATAC GTA^TAC
Bsu36I 61 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1090 ACCGGC [GA]^CCGG[CT]
ClaI 97 ATCGAT AT^CGAT
EaeI 987 TGGCCA [CT]^GGCC[GA]
EarI 163 GAAGAG CTCTTC(1/4)
Eco81I 61 CCTCAGG CC^T[ACGT]AGG
HaeII 958 AGCGCC [GA]GCGC^[CT]
Hin1I 893 GACGTC G[GA]^CG[CT]C
Ksp632I 163 GAAGAG CTCTTC(1/4)
MluNI 987 TGGCCA TGG^CCA
MscI 987 TGGCCA TGG^CCA
MseI 25 TTAA T^TAA
MslI 850 CACATCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PleI 560 GAGTC GAGTC(4/5)
PmaCI 1071 CACGTG CAC^GTG
PmlI 1071 CACGTG CAC^GTG
PstI 323 CTGCAG CTGCA^G
ScaI 196 AGTACT AGT^ACT
SfcI 323 CTGCAG C^T[GA][CT]AG
SspBI 47 TGTACA T^GTACA
Tru9I 25 TTAA T^TAA
XcmI 869 CCAAGGGAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 180 GAATTT [GA]^AATT[CT]
607 GAATTT
ApoI 180 GAATTT [GA]^AATT[CT]
607 GAATTT
BanI 431 GGCACC G^G[CT][GA]CC
839 GGCACC
BbsI 688 GAAGAC GAAGAC(2/6)
1076 GTCTTC
BcgI 930 CGACTTTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
515 GCAGGGACATCG
BpuAI 688 GAAGAC GAAGAC(2/6)
1076 GTCTTC
BseRI 787 GAGGAG GAGGAG(10/8)
811 GAGGAG
BspLU11I 50 ACATGT A^CATGT
227 ACATGT
BstF5I 852 CATCC GGATG(2/0)
1065 CATCC
CfoI 120 GCGC GCG^C
959 GCGC
EcoT14I 773 CCAAGG C^C[AT][AT]GG
869 CCAAGG
FauI 571 GCGGG CCCGC(4/6)
783 GCGGG
FokI 852 CATCC GGATG(9/13)
1065 CATCC
HhaI 120 GCGC GCG^C
959 GCGC
HinP1I 120 GCGC G^CGC
959 GCGC
MaeII 894 ACGT A^CGT
1072 ACGT
MwoI 499 GCAGACAGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
940 GCTGGTGTAGC
NspI 50 ACATGT [GA]CATG^[CT]
227 ACATGT
PpuMI 257 GGGACCC [GA]G^G[AT]CC[CT]
272 AGGACCC
SfaNI 35 GCATC GCATC(5/9)
675 GCATC
StuI 319 AGGCCT AGG^CCT
876 AGGCCT
StyI 773 CCAAGG C^C[AT][AT]GG
869 CCAAGG
TfiI 632 GAATC G^A[AT]TC
905 GATTC
Tsp45I 224 GTGAC ^GT[GC]AC
1051 GTGAC
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AfaI AflIII AlwI AvaII
BanII BbvI BcnI BmyI BpmI
BsaJI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstNI Cac8I Cfr13I
DdeI DpnII DraII EcoNI EcoO109I
EcoRII Fnu4HI HaeIII HapII HinfI
HpaII HphI ItaI MaeIII MboI
MboII MnlI MspI MvaI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI TaqI TseI Tsp509I
TspRI TthHB8I