(Circular) (Six-base) MAPSORT of: pFN21AB3507.seq Check: 3490 from: 1 to: 5824 pFN21AB3507 5824 bp With 182 enzymes: SgfI * October 20, 2009 10:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2038 2038 Size: 5824 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2902 3634 278 Size: 53 83 186 936 1366 732 2468 Fragments arranged by size: 2468 1366 936 732 186 83 53 AccI GT'mk_AC Cuts at: 1937 2414 3167 1937 Size: 477 753 4594 Fragments arranged by size: 4594 753 477 Acc65I G'GTAC_C Cuts at: 3145 3145 Size: 5824 AclI AA'CG_TT Cuts at: 1563 3951 4324 1563 Size: 2388 373 3063 Fragments arranged by size: 3063 2388 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2755 2825 3887 5271 5539 1632 Size: 1123 70 1062 1384 268 1917 Fragments arranged by size: 1917 1384 1123 1062 268 70 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5627 AflIII A'CryG_T Cuts at: 1203 2055 2232 3077 4723 5700 1203 Size: 852 177 845 1646 977 1327 Fragments arranged by size: 1646 1327 977 852 845 177 AgeI A'CCGG_T Cuts at: 4655 4655 Size: 5824 AhdI GACnn_n'nnGTC Cuts at: 4553 4553 Size: 5824 AleI CACnn'nnGTG Cuts at: 1166 2859 1166 Size: 1693 4131 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5824 AlwNI CAG_nnn'CTG Cuts at: 3567 5139 3567 Size: 1572 4252 ApaI G_GGCC'C Cuts at: 2475 2475 Size: 5824 ApaLI G'TGCA_C Cuts at: 3881 5037 3881 Size: 1156 4668 ApoI r'AATT_y Cuts at: 1493 2185 2612 3133 3359 3511 5620 1493 Size: 692 427 521 226 152 2109 1697 Fragments arranged by size: 2109 1697 692 521 427 226 152 AseI AT'TA_AT Cuts at: 160 1032 4378 160 Size: 872 3346 1606 Fragments arranged by size: 3346 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 AvaI C'yCGr_G Cuts at: 1870 1943 3149 1870 Size: 73 1206 4545 Fragments arranged by size: 4545 1206 73 BamHI G'GATC_C Cuts at: 3154 3521 3154 Size: 367 5457 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2436 2844 3145 4600 618 Size: 325 217 627 649 408 301 1455 1842 Fragments arranged by size: 1842 1455 649 627 408 325 301 217 BanII G_rGCy'C Cuts at: 1391 2157 2475 3108 3143 1391 Size: 766 318 633 35 4072 Fragments arranged by size: 4072 766 633 318 35 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2700 3074 928 Size: 971 801 374 3678 Fragments arranged by size: 3678 971 801 374 BbvCI CC'TCA_GC Cuts at: 2014 2014 Size: 5824 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2509 2543 2924 2958 4015 4049 1903 Size: 34 572 34 381 34 1057 34 3678 Fragments arranged by size: 3678 1057 572 381 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3716 4926 3716 Size: 1210 4614 BclI T'GATC_A Cuts at: 1548 1548 Size: 5824 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4435 136 Size: 107 122 71 631 605 2763 1525 Fragments arranged by size: 2763 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5819 5819 Size: 5824 BlpI GC'TnA_GC Cuts at: 3241 3241 Size: 5824 Bme1580I G_kGCm'C Cuts at: 1790 2439 2475 3885 5041 1790 Size: 649 36 1410 1156 2573 Fragments arranged by size: 2573 1410 1156 649 36 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5824 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2599 3555 4513 Size: 416 53 83 399 24 1182 956 958 Cuts at: 4513 442 Size: 1753 Fragments arranged by size: 1753 1182 958 956 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5824 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2672 2672 Size: 5824 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2133 2182 2583 4484 1239 Size: 894 49 401 1901 2579 Fragments arranged by size: 2579 1901 894 401 49 Bpu10I CC'TnA_GC Cuts at: 2014 2724 2014 Size: 710 5114 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3949 4814 5112 3949 Size: 865 298 4661 Fragments arranged by size: 4661 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2327 4487 882 Size: 617 828 2160 2219 Fragments arranged by size: 2219 2160 828 617 BsaAI yAC'GTr Cuts at: 493 1204 3078 3615 493 Size: 711 1874 537 2702 Fragments arranged by size: 2702 1874 711 537 BsaBI GATnn'nnATC Cuts at: 5818 5818 Size: 5824 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2899 3631 4013 4648 Size: 53 83 186 936 1366 732 382 635 Cuts at: 4648 275 Size: 1451 Fragments arranged by size: 1451 1366 936 732 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4257 4655 4929 5076 5487 4257 Size: 398 274 147 411 4594 Fragments arranged by size: 4594 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2396 2426 1178 Size: 30 165 30 993 30 4576 Fragments arranged by size: 4576 993 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2807 2831 1186 Size: 1621 24 4179 Fragments arranged by size: 4179 1621 24 BseYI C'CCAG_C Cuts at: 5027 5523 5027 Size: 496 5328 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2772 1163 Size: 573 1036 4215 Fragments arranged by size: 4215 1036 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3274 4035 5063 1540 Size: 275 186 1273 761 1028 2301 Fragments arranged by size: 2301 1273 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3885 3970 5041 3885 Size: 85 1071 4668 Fragments arranged by size: 4668 1071 85 BsmI GAATG_Cn' Cuts at: 2976 3335 3428 2976 Size: 359 93 5372 Fragments arranged by size: 5372 359 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2157 2439 2475 3108 3143 3885 3970 Size: 399 367 282 36 633 35 742 85 Cuts at: 3970 5041 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 633 399 367 282 85 36 35 BspEI T'CCGG_A Cuts at: 5487 5487 Size: 5824 BspHI T'CATG_A Cuts at: 3713 5443 3713 Size: 1730 4094 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2038 3179 844 Size: 1194 1141 3489 Fragments arranged by size: 3489 1194 1141 BsrBI CCG'CTC Cuts at: 1394 1823 3711 1394 Size: 429 1888 3507 Fragments arranged by size: 3507 1888 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4319 4493 65 Size: 1183 3071 174 1396 Fragments arranged by size: 3071 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3095 4468 4655 1700 Size: 110 1285 1373 187 2869 Fragments arranged by size: 2869 1373 1285 187 110 BsrGI T'GTAC_A Cuts at: 96 2052 96 Size: 1956 3868 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5824 BssSI C'ACGA_G Cuts at: 2240 3884 4896 2240 Size: 1644 1012 3168 Fragments arranged by size: 3168 1644 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5824 BstBI TT'CG_AA Cuts at: 4628 4628 Size: 5824 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5824 BstXI CCAn_nnnn'nTGG Cuts at: 2164 2164 Size: 5824 BstYI r'GATC_y Cuts at: 1972 3154 3521 3906 3923 5375 5461 5473 5819 Size: 1182 367 385 17 1452 86 12 346 Cuts at: 5819 1972 Size: 1977 Fragments arranged by size: 1977 1452 1182 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2415 2415 Size: 5824 Bsu36I CC'TnA_GG Cuts at: 2067 2067 Size: 5824 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5272 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3114 487 Size: 683 56 1888 3197 Fragments arranged by size: 3197 1888 683 56 BtsI GCAGTG_nn' Cuts at: 3340 4134 4154 3340 Size: 794 20 5010 Fragments arranged by size: 5010 794 20 ClaI AT'CG_AT Cuts at: 1554 2103 1554 Size: 549 5275 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5789 DraI TTT'AAA Cuts at: 3128 3128 Size: 5824 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4831 809 Size: 507 3515 1802 Fragments arranged by size: 3515 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2992 3271 4160 Size: 54 1425 50 275 18 1162 279 889 Cuts at: 4160 5789 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1162 889 279 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3271 1537 Size: 275 1459 4090 Fragments arranged by size: 4090 1459 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2163 3754 1353 Size: 810 1591 3423 Fragments arranged by size: 3423 1591 810 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4411 4785 4931 387 Size: 1064 138 2822 374 146 1280 Fragments arranged by size: 2822 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2133 2182 2583 2755 2825 3887 4484 Size: 393 501 49 401 172 70 1062 597 Cuts at: 4484 5271 5539 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1062 787 597 501 401 393 268 172 70 49 EcoNI CCTnn'n_nnAGG Cuts at: 2066 2518 2631 2066 Size: 452 113 5259 Fragments arranged by size: 5259 452 113 EcoO109I rG'GnC_Cy Cuts at: 2263 2278 2471 2472 2263 Size: 15 193 1 5615 Fragments arranged by size: 5615 193 15 1 EcoRI G'AATT_C Cuts at: 3133 3511 3133 Size: 378 5446 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3028 3028 Size: 5824 FspI TGC'GCA Cuts at: 4330 4330 Size: 5824 HaeII r_GCGC'y Cuts at: 1678 2967 4971 1678 Size: 1289 2004 2531 Fragments arranged by size: 2531 2004 1289 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4465 4497 4539 4571 1373 Size: 32 135 32 2893 32 42 32 2626 Fragments arranged by size: 2893 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3168 3414 669 Size: 988 281 1230 246 3079 Fragments arranged by size: 3079 1230 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5824 HpaI GTT'AAC Cuts at: 3414 3414 Size: 5824 KpnI G_GTAC'C Cuts at: 3149 3149 Size: 5824 MfeI C'AATT_G Cuts at: 3423 3423 Size: 5824 MluI A'CGCG_T Cuts at: 5700 5700 Size: 5824 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2247 2481 2949 4938 5122 1305 Size: 942 234 468 1989 184 2007 Fragments arranged by size: 2007 1989 942 468 234 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2994 10 Size: 54 1425 343 1162 2840 Fragments arranged by size: 2840 1425 1162 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2859 3782 4141 4300 518 Size: 648 1693 923 359 159 2042 Fragments arranged by size: 2042 1693 923 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3240 3917 5065 5310 1606 Size: 1634 677 1148 245 2120 Fragments arranged by size: 2120 1634 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5272 NdeI CA'TA_TG Cuts at: 387 387 Size: 5824 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5824 NotI GC'GGCC_GC Cuts at: 3271 3271 Size: 5824 NspI r_CATG'y Cuts at: 1132 1229 2059 2236 3182 4727 1132 Size: 97 830 177 946 1545 2229 Fragments arranged by size: 2229 1545 946 830 177 97 PasI CC'CwG_GG Cuts at: 1348 2468 2476 1348 Size: 1120 8 4696 Fragments arranged by size: 4696 1120 8 PciI A'CATG_T Cuts at: 2055 2232 4723 2055 Size: 177 2491 3156 Fragments arranged by size: 3156 2491 177 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5824 PmeI GTTT'AAAC Cuts at: 3128 3128 Size: 5824 PmlI CAC'GTG Cuts at: 3078 3078 Size: 5824 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3893 3925 5406 5438 3893 Size: 32 1481 32 4279 Fragments arranged by size: 4279 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2263 2278 2263 Size: 15 5809 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5824 PsiI TTA'TAA Cuts at: 3394 3394 Size: 5824 PspOMI G'GGCC_C Cuts at: 2471 2471 Size: 5824 PstI C_TGCA'G Cuts at: 830 2332 3176 830 Size: 1502 844 3478 Fragments arranged by size: 3478 1502 844 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5824 SalI G'TCGA_C Cuts at: 1936 3166 1936 Size: 1230 4594 SanDI GG'GwC_CC Cuts at: 2263 2263 Size: 5824 SbfI CC_TGCA'GG Cuts at: 3176 3176 Size: 5824 ScaI AGT'ACT Cuts at: 2203 4072 5784 2203 Size: 1869 1712 2243 Fragments arranged by size: 2243 1869 1712 SfcI C'TryA_G Cuts at: 826 1045 2328 3172 4307 4988 5179 826 Size: 219 1283 844 1135 681 191 1471 Fragments arranged by size: 1471 1283 1135 844 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5824 SmaI CCC'GGG Cuts at: 1872 3151 1872 Size: 1279 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3928 4829 5091 820 Size: 197 926 1985 901 262 1553 Fragments arranged by size: 1985 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3615 493 Size: 3122 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5824 SphI G_CATG'C Cuts at: 1132 3182 1132 Size: 2050 3774 SspI AAT'ATT Cuts at: 5 52 3748 5 Size: 47 3696 2081 Fragments arranged by size: 3696 2081 47 StuI AGG'CCT Cuts at: 1517 2326 2883 1517 Size: 809 557 4458 Fragments arranged by size: 4458 809 557 StyI C'CwwG_G Cuts at: 513 1065 2778 2874 3263 513 Size: 552 1713 96 389 3074 Fragments arranged by size: 3074 1713 552 389 96 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2650 3851 3868 4021 4206 4651 325 Size: 1255 1070 1201 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1201 1070 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2052 2201 2313 Size: 274 80 33 51 1443 75 149 112 Cuts at: 2313 2808 3114 4070 5782 96 Size: 495 306 956 1712 138 Fragments arranged by size: 1712 1443 956 495 306 274 149 138 112 80 75 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5784 5816 5784 Size: 32 5792 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5824 XbaI T'CTAG_A Cuts at: 3160 3160 Size: 5824 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2881 2881 Size: 5824 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5824 XmaI C'CCGG_G Cuts at: 1870 3149 1870 Size: 1279 4545 XmnI GAAnn'nnTTC Cuts at: 3953 3953 Size: 5824 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2900 3632 276 Size: 53 83 186 936 1366 732 2468 Fragments arranged by size: 2468 1366 936 732 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI