(Circular) (Six-base) MAPSORT of: pFN21AE1390.seq Check: 2009 from: 1 to: 5836 pFN21AE1390 5836 bp With 182 enzymes: SgfI * August 16, 2010 14:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3646 278 Size: 53 83 186 936 2110 2468 Fragments arranged by size: 2468 2110 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3179 1937 Size: 1242 4594 Acc65I G'GTAC_C Cuts at: 3157 3157 Size: 5836 AclI AA'CG_TT Cuts at: 1563 3963 4336 1563 Size: 2400 373 3063 Fragments arranged by size: 3063 2400 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2231 3899 5283 5551 1632 Size: 599 1668 1384 268 1917 Fragments arranged by size: 1917 1668 1384 599 268 AfeI AGC'GCT Cuts at: 2771 2771 Size: 5836 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5639 AflIII A'CryG_T Cuts at: 1203 4735 5712 1203 Size: 3532 977 1327 Fragments arranged by size: 3532 1327 977 AgeI A'CCGG_T Cuts at: 4667 4667 Size: 5836 AhdI GACnn_n'nnGTC Cuts at: 4565 4565 Size: 5836 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5836 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5836 AlwNI CAG_nnn'CTG Cuts at: 3579 5151 3579 Size: 1572 4264 ApaLI G'TGCA_C Cuts at: 3893 5049 3893 Size: 1156 4680 ApoI r'AATT_y Cuts at: 1493 3145 3371 3523 5632 1493 Size: 1652 226 152 2109 1697 Fragments arranged by size: 2109 1697 1652 226 152 AseI AT'TA_AT Cuts at: 160 1032 4390 160 Size: 872 3358 1606 Fragments arranged by size: 3358 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 AvaI C'yCGr_G Cuts at: 1870 1943 2303 3161 1870 Size: 73 360 858 4545 Fragments arranged by size: 4545 858 360 73 BamHI G'GATC_C Cuts at: 3166 3533 3166 Size: 367 5469 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2127 2268 2915 3157 4612 Size: 325 217 627 340 141 647 242 1455 Cuts at: 4612 618 Size: 1842 Fragments arranged by size: 1842 1455 647 627 340 325 242 217 141 BanII G_rGCy'C Cuts at: 1391 2292 3041 3155 1391 Size: 901 749 114 4072 Fragments arranged by size: 4072 901 749 114 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2092 928 Size: 971 193 4672 Fragments arranged by size: 4672 971 193 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3115 3149 4027 4061 1903 Size: 34 1178 34 878 34 3678 Fragments arranged by size: 3678 1178 878 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2226 3728 4938 2226 Size: 1502 1210 3124 Fragments arranged by size: 3124 1502 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5836 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2420 4447 136 Size: 107 122 71 631 605 748 2027 1525 Fragments arranged by size: 2027 1525 748 631 605 122 107 71 BglII A'GATC_T Cuts at: 2262 5831 2262 Size: 3569 2267 BlpI GC'TnA_GC Cuts at: 3253 3253 Size: 5836 Bme1580I G_kGCm'C Cuts at: 1790 3897 5053 1790 Size: 2107 1156 2573 Fragments arranged by size: 2573 2107 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5836 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2254 3567 4525 Size: 416 53 83 399 24 837 1313 958 Cuts at: 4525 442 Size: 1753 Fragments arranged by size: 1753 1313 958 837 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5836 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2738 2931 4496 1239 Size: 1499 193 1565 2579 Fragments arranged by size: 2579 1565 1499 193 Bpu10I CC'TnA_GC Cuts at: 3009 3009 Size: 5836 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3961 4826 5124 3961 Size: 865 298 4673 Fragments arranged by size: 4673 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2015 2378 4499 882 Size: 617 516 363 2121 2219 Fragments arranged by size: 2219 2121 617 516 363 BsaAI yAC'GTr Cuts at: 493 1204 2170 2410 3627 493 Size: 711 966 240 1217 2702 Fragments arranged by size: 2702 1217 966 711 240 BsaBI GATnn'nnATC Cuts at: 5830 5830 Size: 5836 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3643 4025 4660 275 Size: 53 83 186 936 2110 382 635 1451 Fragments arranged by size: 2110 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4269 4667 4941 5088 5499 4269 Size: 398 274 147 411 4606 Fragments arranged by size: 4606 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2127 2157 1178 Size: 30 165 30 724 30 4857 Fragments arranged by size: 4857 724 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2033 2321 2701 1186 Size: 847 288 380 4321 Fragments arranged by size: 4321 847 380 288 BseYI C'CCAG_C Cuts at: 5039 5535 5039 Size: 496 5340 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5263 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3286 4047 5075 1540 Size: 275 186 1285 761 1028 2301 Fragments arranged by size: 2301 1285 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2292 2313 3897 3982 5053 2292 Size: 21 1584 85 1071 3075 Fragments arranged by size: 3075 1584 1071 85 21 BsmI GAATG_Cn' Cuts at: 3347 3440 3347 Size: 93 5743 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2292 2313 3041 3155 3897 3982 5053 Size: 399 502 21 728 114 742 85 1071 Cuts at: 5053 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 728 502 399 114 85 21 BspEI T'CCGG_A Cuts at: 5499 5499 Size: 5836 BspHI T'CATG_A Cuts at: 2577 2856 3725 5455 2577 Size: 279 869 1730 2958 Fragments arranged by size: 2958 1730 869 279 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3191 844 Size: 2347 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3723 1394 Size: 429 1900 3507 Fragments arranged by size: 3507 1900 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2770 4331 4505 65 Size: 1183 1522 1561 174 1396 Fragments arranged by size: 1561 1522 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2066 4480 4667 1700 Size: 110 256 2414 187 2869 Fragments arranged by size: 2869 2414 256 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5836 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5836 BssSI C'ACGA_G Cuts at: 3896 4908 3896 Size: 1012 4824 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5836 BstBI TT'CG_AA Cuts at: 4640 4640 Size: 5836 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5836 BstXI CCAn_nnnn'nTGG Cuts at: 2032 2410 2032 Size: 378 5458 BstYI r'GATC_y Cuts at: 1972 2262 2545 2583 3166 3533 3918 3935 5387 Size: 290 283 38 583 367 385 17 1452 Cuts at: 5387 5473 5485 5831 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 583 385 367 346 290 283 86 38 17 12 BtgI C'CryG_G Cuts at: 513 1065 2123 513 Size: 552 1058 4226 Fragments arranged by size: 4226 1058 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2094 487 Size: 683 56 868 4229 Fragments arranged by size: 4229 868 683 56 BtsI GCAGTG_nn' Cuts at: 2430 2616 2961 3352 4146 4166 2430 Size: 186 345 391 794 20 4100 Fragments arranged by size: 4100 794 391 345 186 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5836 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2443 2478 529 Size: 35 1879 35 3887 Fragments arranged by size: 3887 1879 35 35 DraI TTT'AAA Cuts at: 3140 3140 Size: 5836 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4843 809 Size: 507 3527 1802 Fragments arranged by size: 3527 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2412 2446 2691 Size: 54 1425 50 275 18 582 34 245 Cuts at: 2691 2743 3283 4172 5801 8 Size: 52 540 889 1629 43 Fragments arranged by size: 1629 1425 889 582 540 275 245 54 52 50 43 34 18 EagI C'GGCC_G Cuts at: 1537 1812 3283 1537 Size: 275 1471 4090 Fragments arranged by size: 4090 1471 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2710 2717 2798 3766 1353 Size: 1357 7 81 968 3423 Fragments arranged by size: 3423 1357 968 81 7 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4423 4797 4943 387 Size: 1064 138 2834 374 146 1280 Fragments arranged by size: 2834 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2290 2290 Size: 5836 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2231 2738 2931 3899 4496 5283 5551 Size: 393 599 507 193 968 597 787 268 Cuts at: 5551 1239 Size: 1524 Fragments arranged by size: 1524 968 787 599 597 507 393 268 193 EcoO109I rG'GnC_Cy Cuts at: 2332 2560 2884 2332 Size: 228 324 5284 Fragments arranged by size: 5284 324 228 EcoRI G'AATT_C Cuts at: 3145 3523 3145 Size: 378 5458 EcoRV GAT'ATC Cuts at: 2875 2875 Size: 5836 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2086 2086 Size: 5836 FspI TGC'GCA Cuts at: 4342 4342 Size: 5836 HaeII r_GCGC'y Cuts at: 1678 2773 4983 1678 Size: 1095 2210 2531 Fragments arranged by size: 2531 2210 1095 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2033 2065 2986 3018 4477 Size: 32 135 32 461 32 921 32 1459 Cuts at: 4477 4509 4551 4583 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1459 921 461 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3180 3426 669 Size: 988 281 1242 246 3079 Fragments arranged by size: 3079 1242 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5836 HpaI GTT'AAC Cuts at: 3426 3426 Size: 5836 KpnI G_GTAC'C Cuts at: 3161 3161 Size: 5836 MfeI C'AATT_G Cuts at: 3435 3435 Size: 5836 MluI A'CGCG_T Cuts at: 5712 5712 Size: 5836 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4950 5134 1305 Size: 3645 184 2007 Fragments arranged by size: 3645 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2414 2693 2745 10 Size: 54 1425 343 582 279 52 3101 Fragments arranged by size: 3101 1425 582 343 279 54 52 MslI CAynn'nnrTG Cuts at: 518 1166 2408 2813 2921 3794 4153 4312 518 Size: 648 1242 405 108 873 359 159 2042 Fragments arranged by size: 2042 1242 873 648 405 359 159 108 MspA1I CmG'CkG Cuts at: 1606 2125 2338 2699 2778 3252 3929 5077 5322 Size: 519 213 361 79 474 677 1148 245 Cuts at: 5322 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 519 474 361 245 213 79 NaeI GCC'GGC Cuts at: 2068 2068 Size: 5836 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5284 NdeI CA'TA_TG Cuts at: 387 387 Size: 5836 NgoMIV G'CCGG_C Cuts at: 2066 2066 Size: 5836 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5836 NotI GC'GGCC_GC Cuts at: 3283 3283 Size: 5836 NspI r_CATG'y Cuts at: 1132 1229 3194 4739 1132 Size: 97 1965 1545 2229 Fragments arranged by size: 2229 1965 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5836 PciI A'CATG_T Cuts at: 4735 4735 Size: 5836 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5836 PmeI GTTT'AAAC Cuts at: 3140 3140 Size: 5836 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3905 3937 5418 5450 3905 Size: 32 1481 32 4291 Fragments arranged by size: 4291 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2560 2884 2560 Size: 324 5512 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5836 PsiI TTA'TAA Cuts at: 3406 3406 Size: 5836 PstI C_TGCA'G Cuts at: 830 3188 830 Size: 2358 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5836 PvuII CAG'CTG Cuts at: 2699 2699 Size: 5836 SacI G_AGCT'C Cuts at: 2292 2292 Size: 5836 SacII CC_GC'GG Cuts at: 2126 2126 Size: 5836 SalI G'TCGA_C Cuts at: 1936 3178 1936 Size: 1242 4594 SapI GCTCTTCn'nnn_ Cuts at: 2717 2717 Size: 5836 SbfI CC_TGCA'GG Cuts at: 3188 3188 Size: 5836 ScaI AGT'ACT Cuts at: 4084 5796 4084 Size: 1712 4124 SfcI C'TryA_G Cuts at: 826 1045 3184 4319 5000 5191 826 Size: 219 2139 1135 681 191 1471 Fragments arranged by size: 2139 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5836 SmaI CCC'GGG Cuts at: 1872 3163 1872 Size: 1291 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3940 4841 5103 820 Size: 197 926 1997 901 262 1553 Fragments arranged by size: 1997 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2170 3627 493 Size: 1677 1457 2702 Fragments arranged by size: 2702 1677 1457 SpeI A'CTAG_T Cuts at: 152 152 Size: 5836 SphI G_CATG'C Cuts at: 1132 3194 1132 Size: 2062 3774 SspI AAT'ATT Cuts at: 5 52 3760 5 Size: 47 3708 2081 Fragments arranged by size: 3708 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5836 StyI C'CwwG_G Cuts at: 513 1065 2346 3275 513 Size: 552 1281 929 3074 Fragments arranged by size: 3074 1281 929 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3863 3880 4033 4218 4663 325 Size: 1255 2283 17 153 185 445 1498 Fragments arranged by size: 2283 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4082 5794 96 Size: 274 80 33 51 1443 2105 1712 138 Fragments arranged by size: 2105 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2127 2159 5796 5828 2127 Size: 32 3637 32 2135 Fragments arranged by size: 3637 2135 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5836 XbaI T'CTAG_A Cuts at: 3172 3172 Size: 5836 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2539 2539 Size: 5836 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5836 XmaI C'CCGG_G Cuts at: 1870 3161 1870 Size: 1291 4545 XmnI GAAnn'nnTTC Cuts at: 3965 3965 Size: 5836 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3644 276 Size: 53 83 186 936 2110 2468 Fragments arranged by size: 2468 2110 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SanDI SexAI SfiI SfoI SrfI SwaI