################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## AatII AccI AclI AcyI AflII AflIII AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BbeI BbrPI BbvCI BciVI BclI BfrI BglI BlnI BpmI BsaHI BsaI BsiEI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrGI BssHII Bst1107I BstBI BstZ17I ClaI DraI EagI Eam1105I EclXI Eco52I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I KasI KspI MfeI MluI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI SacI SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SpeI Sse8387I SspBI SspI SwaI Tth111I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### Acc65I 1773 GGTACC G^GTACC AccIII 1409 TCCGGA T^CCGGA AcsI 1029 AAATTC [GA]^AATT[CT] AfaI 1774 GTAC GT^AC AocI 297 CCTGAGG CC^T[ACGT]AGG Aor51HI 1642 AGCGCT AGC^GCT ApaI 1273 GGGCCC GGGCC^C ApoI 1029 AAATTC [GA]^AATT[CT] Asp718I 1773 GGTACC G^GTACC AvaI 385 CTCGGG C^[CT]CG[GA]G BamHI 1664 GGATCC G^GATCC BbsI 1267 GAAGAC GAAGAC(2/6) BpuAI 1267 GAAGAC GAAGAC(2/6) BsaAI 65 TACGTA [CT]AC^GT[GA] BsaWI 1409 TCCGGA [AT]^CCGG[AT] BseAI 1409 TCCGGA T^CCGGA BseRI 664 GAGGAG GAGGAG(10/8) BsoBI 385 CTCGGG C^[CT]CG[GA]G BspEI 1409 TCCGGA T^CCGGA BsrFI 412 ACCGGC [GA]^CCGG[CT] BstAPI 27 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 297 CCTGAGG CC^T[ACGT]AGG Cfr10I 412 ACCGGC [GA]^CCGG[CT] CpoI 1067 CGGACCG CG^G[AT]CCG DraIII 655 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1456 GACTTCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1488 GAAGAG CTCTTC(1/4) Eco47III 1642 AGCGCT AGC^GCT Eco81I 297 CCTGAGG CC^T[ACGT]AGG HgaI 874 GACGC GACGC(5/10) HincII 1765 GTTAAC GT[CT]^[GA]AC HindII 1765 GTTAAC GT[CT]^[GA]AC HindIII 344 AAGCTT A^AGCTT HpaI 1765 GTTAAC GTT^AAC KpnI 1773 GGTACC GGTAC^C Ksp632I 1488 GAAGAG CTCTTC(1/4) MroI 1409 TCCGGA T^CCGGA MseI 1766 TTAA T^TAA NcoI 497 CCATGG C^CATGG PpuMI 182 AGGACCC [GA]G^G[AT]CC[CT] PvuII 1793 CAGCTG CAG^CTG RsaI 1774 GTAC GT^AC RsrII 1067 CGGACCG CG^G[AT]CCG SapI 1488 GAAGAGC GCTCTTC(1/4) SexAI 1502 ACCAGGT A^CC[AT]GGT SnaBI 65 TACGTA TAC^GTA Tru9I 1766 TTAA T^TAA Tsp509I 1030 AATT ^AATT XbaI 543 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AlwNI 28 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG 967 CAGGCACTG AspHI 214 GTGCTC G[AT]GC[AT]^C 1060 GAGCAC BlpI 799 GCTGAGC GC^T[ACGT]AGC 1795 GCTGAGC Bpu1102I 799 GCTGAGC GC^T[ACGT]AGC 1795 GCTGAGC BsaBI 594 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1578 GATCAACATC BsgI 1534 CTGCAC GTGCAG(16/14) 1721 GTGCAG BsiHKAI 214 GTGCTC G[AT]GC[AT]^C 1060 GAGCAC BssSI 1537 CACGAG CACGAG(-5/-1) 1646 CTCGTG BstEII 1506 GGTGACC G^GT[ACGT]ACC 1527 GGTGACC BstPI 1506 GGTGACC G^GT[ACGT]ACC 1527 GGTGACC BstXI 638 CCATCATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 788 CCAAAAACATGG CelII 799 GCTGAGC GC^T[ACGT]AGC 1795 GCTGAGC DsaI 497 CCATGG C^C[GA][CT]GG 1673 CCGTGG EcoO65I 1506 GGTGACC G^GT[ACGT]ACC 1527 GGTGACC HaeII 127 AGCGCC [GA]GCGC^[CT] 1642 AGCGCT MamI 594 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1578 GATCAACATC NspI 633 GCATGC [GA]CATG^[CT] 905 GCATGC PflMI 1342 CCAAGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1777 CCAAGAAGTGG PleI 443 GAGTC GAGTC(4/5) 763 GAGTC SphI 633 GCATGC GCATG^C 905 GCATGC TfiI 192 GAATC G^A[AT]TC 1197 GATTC Van91I 1342 CCAAGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1777 CCAAGAAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AccII AciI AluI AlwI AvaII BalI BanI BanII BbvI BcgI BcnI BfaI BglII BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PstI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI TseI Tsp45I TspRI TthHB8I XcmI XhoII