(Circular) (Six-base) MAPSORT of: pFN21AE4541.seq Check: 7944 from: 1 to: 6574 pFN21AE4541 6574 bp With 182 enzymes: SgfI * March 28, 2012 14:11 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4384 278 Size: 53 83 186 936 2848 2468 Fragments arranged by size: 2848 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3917 1937 Size: 1980 4594 Acc65I G'GTAC_C Cuts at: 3778 3895 3778 Size: 117 6457 AclI AA'CG_TT Cuts at: 1563 4701 5074 1563 Size: 3138 373 3063 Fragments arranged by size: 3138 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2286 3512 3590 3830 4637 6021 6289 1632 Size: 654 1226 78 240 807 1384 268 1917 Fragments arranged by size: 1917 1384 1226 807 654 268 240 78 AfeI AGC'GCT Cuts at: 3649 3649 Size: 6574 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6377 AflIII A'CryG_T Cuts at: 1203 5473 6450 1203 Size: 4270 977 1327 Fragments arranged by size: 4270 1327 977 AgeI A'CCGG_T Cuts at: 5405 5405 Size: 6574 AhdI GACnn_n'nnGTC Cuts at: 5303 5303 Size: 6574 AleI CACnn'nnGTG Cuts at: 1166 2360 1166 Size: 1194 5380 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6574 AlwNI CAG_nnn'CTG Cuts at: 2038 2977 4317 5889 2038 Size: 939 1340 1572 2723 Fragments arranged by size: 2723 1572 1340 939 ApaI G_GGCC'C Cuts at: 3282 3282 Size: 6574 ApaLI G'TGCA_C Cuts at: 4631 5787 4631 Size: 1156 5418 ApoI r'AATT_y Cuts at: 1493 3034 3883 4109 4261 6370 1493 Size: 1541 849 226 152 2109 1697 Fragments arranged by size: 2109 1697 1541 849 226 152 AseI AT'TA_AT Cuts at: 160 1032 5128 160 Size: 872 4096 1606 Fragments arranged by size: 4096 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6574 AvaI C'yCGr_G Cuts at: 1870 1943 2390 3899 1870 Size: 73 447 1509 4545 Fragments arranged by size: 4545 1509 447 73 BamHI G'GATC_C Cuts at: 3669 3904 4271 3669 Size: 235 367 5972 Fragments arranged by size: 5972 367 235 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2331 3151 3778 3895 5350 Size: 325 217 627 544 820 627 117 1455 Cuts at: 5350 618 Size: 1842 Fragments arranged by size: 1842 1455 820 627 627 544 325 217 117 BanII G_rGCy'C Cuts at: 1391 2108 2489 3282 3893 1391 Size: 717 381 793 611 4072 Fragments arranged by size: 4072 793 717 611 381 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3279 928 Size: 971 1380 4223 Fragments arranged by size: 4223 1380 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2410 2436 2444 2470 3815 3849 4765 Size: 34 473 26 8 26 1345 34 916 Cuts at: 4765 4799 1903 Size: 34 3678 Fragments arranged by size: 3678 1345 916 473 34 34 34 26 26 8 BciVI GTATCCnnnnn_n' Cuts at: 4466 5676 4466 Size: 1210 5364 BclI T'GATC_A Cuts at: 1548 1548 Size: 6574 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5185 136 Size: 107 122 71 631 605 3513 1525 Fragments arranged by size: 3513 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2823 3051 3301 6569 2823 Size: 228 250 3268 2828 Fragments arranged by size: 3268 2828 250 228 BlpI GC'TnA_GC Cuts at: 2805 3801 3991 2805 Size: 996 190 5388 Fragments arranged by size: 5388 996 190 Bme1580I G_kGCm'C Cuts at: 1790 2334 3156 3282 4635 5791 1790 Size: 544 822 126 1353 1156 2573 Fragments arranged by size: 2573 1353 1156 822 544 126 BmgBI CAC'GTC Cuts at: 1318 2121 2362 1318 Size: 803 241 5530 Fragments arranged by size: 5530 803 241 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4305 5263 442 Size: 416 53 83 399 24 2888 958 1753 Fragments arranged by size: 2888 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6574 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5234 1239 Size: 3995 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4699 5564 5862 4699 Size: 865 298 5411 Fragments arranged by size: 5411 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5237 882 Size: 617 3738 2219 Fragments arranged by size: 3738 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2072 4365 493 Size: 711 868 2293 2702 Fragments arranged by size: 2702 2293 868 711 BsaBI GATnn'nnATC Cuts at: 2603 3587 6568 2603 Size: 984 2981 2609 Fragments arranged by size: 2981 2609 984 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4381 4763 5398 275 Size: 53 83 186 936 2848 382 635 1451 Fragments arranged by size: 2848 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3414 5007 5405 5679 5826 6237 3414 Size: 1593 398 274 147 411 3751 Fragments arranged by size: 3751 1593 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2660 2690 3266 3296 1178 Size: 30 165 30 1257 30 576 30 4456 Fragments arranged by size: 4456 1257 576 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2684 1186 Size: 1498 5076 BseYI C'CCAG_C Cuts at: 2461 2915 2924 2986 3527 5777 6273 2461 Size: 454 9 62 541 2250 496 2762 Fragments arranged by size: 2762 2250 541 496 454 62 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3524 3747 1163 Size: 573 1788 223 3990 Fragments arranged by size: 3990 1788 573 223 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4024 4785 5813 1540 Size: 275 186 2023 761 1028 2301 Fragments arranged by size: 2301 2023 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2223 3069 4635 4720 5791 2223 Size: 846 1566 85 1071 3006 Fragments arranged by size: 3006 1566 1071 846 85 BsmI GAATG_Cn' Cuts at: 4085 4178 4085 Size: 93 6481 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2108 2223 2334 2489 3069 3156 3282 Size: 399 318 115 111 155 580 87 126 Cuts at: 3282 3893 4635 4720 5791 1391 Size: 611 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 611 580 399 318 155 126 115 111 87 85 BspEI T'CCGG_A Cuts at: 3414 6237 3414 Size: 2823 3751 BspHI T'CATG_A Cuts at: 4463 6193 4463 Size: 1730 4844 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3929 844 Size: 3085 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4461 1394 Size: 429 2638 3507 Fragments arranged by size: 3507 2638 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2718 2895 3093 3837 5069 5243 65 Size: 1183 1470 177 198 744 1232 174 1396 Fragments arranged by size: 1470 1396 1232 1183 744 198 177 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2417 5218 5405 1700 Size: 110 607 2801 187 2869 Fragments arranged by size: 2869 2801 607 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6574 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6574 BssSI C'ACGA_G Cuts at: 3542 3651 4634 5646 3542 Size: 109 983 1012 4470 Fragments arranged by size: 4470 1012 983 109 BstAPI GCAn_nnn'nTGC Cuts at: 794 2038 794 Size: 1244 5330 BstBI TT'CG_AA Cuts at: 5378 5378 Size: 6574 BstEII G'GTnAC_C Cuts at: 1184 3511 3532 1184 Size: 2327 21 4226 Fragments arranged by size: 4226 2327 21 BstXI CCAn_nnnn'nTGG Cuts at: 2650 2800 2650 Size: 150 6424 BstYI r'GATC_y Cuts at: 1972 2823 2835 2853 3051 3301 3669 3904 4271 Size: 851 12 18 198 250 368 235 367 Cuts at: 4271 4656 4673 6125 6211 6223 6569 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 851 385 368 367 346 250 235 198 86 18 17 12 12 Bsu36I CC'TnA_GG Cuts at: 2303 2303 Size: 6574 BtgI C'CryG_G Cuts at: 513 1065 2004 2502 3678 513 Size: 552 939 498 1176 3409 Fragments arranged by size: 3409 1176 939 552 498 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2995 3103 487 Size: 683 56 1769 108 3958 Fragments arranged by size: 3958 1769 683 108 56 BtsI GCAGTG_nn' Cuts at: 2094 3250 3381 4090 4884 4904 2094 Size: 1156 131 709 794 20 3764 Fragments arranged by size: 3764 1156 794 709 131 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6574 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6539 DraI TTT'AAA Cuts at: 3878 3878 Size: 6574 DraIII CAC_nnn'GTG Cuts at: 2665 2665 Size: 6574 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3467 5581 809 Size: 507 2151 2114 1802 Fragments arranged by size: 2151 2114 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2058 2110 2280 Size: 54 1425 50 275 18 228 52 170 Cuts at: 2280 2364 2541 2689 2979 3210 3552 4021 4910 Size: 84 177 148 290 231 342 469 889 Cuts at: 4910 6539 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 469 342 290 275 231 228 177 170 148 84 54 52 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4021 1537 Size: 275 2209 4090 Fragments arranged by size: 4090 2209 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3488 4504 1353 Size: 2135 1016 3423 Fragments arranged by size: 3423 2135 1016 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2303 5161 5535 5681 387 Size: 1064 138 714 2858 374 146 1280 Fragments arranged by size: 2858 1280 1064 714 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2286 3512 3590 3830 4637 5234 6021 Size: 393 654 1226 78 240 807 597 787 Cuts at: 6021 6289 1239 Size: 268 1524 Fragments arranged by size: 1524 1226 807 787 654 597 393 268 240 78 EcoNI CCTnn'n_nnAGG Cuts at: 2282 2381 3542 2282 Size: 99 1161 5314 Fragments arranged by size: 5314 1161 99 EcoO109I rG'GnC_Cy Cuts at: 2188 2386 3279 3856 2188 Size: 198 893 577 4906 Fragments arranged by size: 4906 893 577 198 EcoRI G'AATT_C Cuts at: 3883 4261 3883 Size: 378 6196 FspI TGC'GCA Cuts at: 5080 5080 Size: 6574 HaeII r_GCGC'y Cuts at: 1678 2136 3651 5721 1678 Size: 458 1515 2070 2531 Fragments arranged by size: 2531 2070 1515 458 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2753 2785 2870 2902 3266 Size: 32 135 32 1181 32 85 32 364 Cuts at: 3266 3298 3704 3736 5215 5247 5289 5321 1373 Size: 32 406 32 1479 32 42 32 2626 Fragments arranged by size: 2626 1479 1181 406 364 135 85 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3772 3918 4164 669 Size: 988 281 1834 146 246 3079 Fragments arranged by size: 3079 1834 988 281 246 146 HindIII A'AGCT_T Cuts at: 748 2349 748 Size: 1601 4973 HpaI GTT'AAC Cuts at: 3772 4164 3772 Size: 392 6182 KpnI G_GTAC'C Cuts at: 3782 3899 3782 Size: 117 6457 MfeI C'AATT_G Cuts at: 4173 4173 Size: 6574 MluI A'CGCG_T Cuts at: 6450 6450 Size: 6574 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2343 3451 5688 5872 1305 Size: 1038 1108 2237 184 2007 Fragments arranged by size: 2237 2007 1108 1038 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2112 2366 2543 2691 2981 Size: 54 1425 343 280 254 177 148 290 Cuts at: 2981 10 Size: 3603 Fragments arranged by size: 3603 1425 343 290 280 254 177 148 54 MslI CAynn'nnrTG Cuts at: 518 1166 2360 2648 2909 2997 3122 3593 3693 Size: 648 1194 288 261 88 125 471 100 Cuts at: 3693 4532 4891 5050 518 Size: 839 359 159 2042 Fragments arranged by size: 2042 1194 839 648 471 359 288 261 159 125 100 88 MspA1I CmG'CkG Cuts at: 1606 2587 3557 3800 3990 4667 5815 6060 1606 Size: 981 970 243 190 677 1148 245 2120 Fragments arranged by size: 2120 1148 981 970 677 245 243 190 NcoI C'CATG_G Cuts at: 513 1065 2004 2502 513 Size: 552 939 498 4585 Fragments arranged by size: 4585 939 552 498 NdeI CA'TA_TG Cuts at: 387 387 Size: 6574 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6574 NotI GC'GGCC_GC Cuts at: 4021 4021 Size: 6574 NspI r_CATG'y Cuts at: 1132 1229 2642 2914 3932 5477 1132 Size: 97 1413 272 1018 1545 2229 Fragments arranged by size: 2229 1545 1413 1018 272 97 PasI CC'CwG_GG Cuts at: 1348 2377 1348 Size: 1029 5545 PciI A'CATG_T Cuts at: 5473 5473 Size: 6574 PflMI CCAn_nnn'nTGG Cuts at: 1108 3353 3788 1108 Size: 2245 435 3894 Fragments arranged by size: 3894 2245 435 PfoI T'CCnGG_A Cuts at: 2817 2817 Size: 6574 PmeI GTTT'AAAC Cuts at: 3878 3878 Size: 6574 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4643 4675 6156 6188 4643 Size: 32 1481 32 5029 Fragments arranged by size: 5029 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2188 2188 Size: 6574 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6574 PsiI TTA'TAA Cuts at: 4144 4144 Size: 6574 PspOMI G'GGCC_C Cuts at: 3278 3278 Size: 6574 PstI C_TGCA'G Cuts at: 830 2043 2132 2592 3164 3926 830 Size: 1213 89 460 572 762 3478 Fragments arranged by size: 3478 1213 762 572 460 89 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6574 PvuII CAG'CTG Cuts at: 3800 3800 Size: 6574 RsrII CG'GwC_CG Cuts at: 3073 3073 Size: 6574 SalI G'TCGA_C Cuts at: 1936 3916 1936 Size: 1980 4594 SapI GCTCTTCn'nnn_ Cuts at: 3488 3488 Size: 6574 SbfI CC_TGCA'GG Cuts at: 3926 3926 Size: 6574 ScaI AGT'ACT Cuts at: 4822 6534 4822 Size: 1712 4862 SexAI A'CCwGG_T Cuts at: 3507 3507 Size: 6574 SfcI C'TryA_G Cuts at: 826 1045 2039 2128 2588 2827 3160 3363 3922 Size: 219 994 89 460 239 333 203 559 Cuts at: 3922 5057 5738 5929 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 994 681 559 460 333 239 219 203 191 89 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6574 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6574 SmaI CCC'GGG Cuts at: 1872 3901 1872 Size: 2029 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4678 5579 5841 820 Size: 197 926 2735 901 262 1553 Fragments arranged by size: 2735 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2072 4365 493 Size: 1579 2293 2702 Fragments arranged by size: 2702 2293 1579 SpeI A'CTAG_T Cuts at: 152 152 Size: 6574 SphI G_CATG'C Cuts at: 1132 2642 2914 3932 1132 Size: 1510 272 1018 3774 Fragments arranged by size: 3774 1510 1018 272 SspI AAT'ATT Cuts at: 5 52 4498 5 Size: 47 4446 2081 Fragments arranged by size: 4446 2081 47 StuI AGG'CCT Cuts at: 1517 2045 2627 2684 2963 3548 1517 Size: 528 582 57 279 585 4543 Fragments arranged by size: 4543 585 582 528 279 57 StyI C'CwwG_G Cuts at: 513 1065 2004 2226 2502 3480 3549 3660 3867 Size: 552 939 222 276 978 69 111 207 Cuts at: 3867 4013 513 Size: 146 3074 Fragments arranged by size: 3074 978 939 552 276 222 207 146 111 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3306 3773 4601 4618 4771 4956 5401 Size: 1255 1726 467 828 17 153 185 445 Cuts at: 5401 325 Size: 1498 Fragments arranged by size: 1726 1498 1255 828 467 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4820 6532 96 Size: 274 80 33 51 1443 2843 1712 138 Fragments arranged by size: 2843 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2649 2660 2681 2692 6534 6566 2649 Size: 11 21 11 3842 32 2657 Fragments arranged by size: 3842 2657 32 21 11 11 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6574 XbaI T'CTAG_A Cuts at: 2548 3910 2548 Size: 1362 5212 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2374 3619 3730 2374 Size: 1245 111 5218 Fragments arranged by size: 5218 1245 111 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6574 XmaI C'CCGG_G Cuts at: 1870 3899 1870 Size: 2029 4545 XmnI GAAnn'nnTTC Cuts at: 4703 4703 Size: 6574 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4382 276 Size: 53 83 186 936 2848 2468 Fragments arranged by size: 2848 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI SacI SacII SanDI SfiI SfoI SrfI SwaI