(Linear) (Six-base) MAPSORT of: KIEE4541.seq Check: 9404 from: 1 to: 1869 KIEE4541.orf With 182 enzymes: SgfI * March 22, 2012 18:19 .. Acc65I G'GTAC_C Cuts at: 0 1773 1869 Size: 1773 96 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 281 1507 1585 1825 1869 Size: 281 1226 78 240 44 Fragments arranged by size: 1226 281 240 78 44 AfeI AGC'GCT Cuts at: 0 1644 1869 Size: 1644 225 AleI CACnn'nnGTG Cuts at: 0 355 1869 Size: 355 1514 AlwNI CAG_nnn'CTG Cuts at: 0 33 972 1869 Size: 33 939 897 Fragments arranged by size: 939 897 33 ApaI G_GGCC'C Cuts at: 0 1277 1869 Size: 1277 592 ApoI r'AATT_y Cuts at: 0 1029 1869 Size: 1029 840 AvaI C'yCGr_G Cuts at: 0 385 1869 Size: 385 1484 BamHI G'GATC_C Cuts at: 0 1664 1869 Size: 1664 205 BanI G'GyrC_C Cuts at: 0 326 1146 1773 1869 Size: 326 820 627 96 Fragments arranged by size: 820 627 326 96 BanII G_rGCy'C Cuts at: 0 103 484 1277 1869 Size: 103 381 793 592 Fragments arranged by size: 793 592 381 103 BbsI GAAGACnn'nnnn_ Cuts at: 0 1274 1869 Size: 1274 595 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 405 431 439 465 1810 1844 1869 Size: 405 26 8 26 1345 34 25 Fragments arranged by size: 1345 405 34 26 26 25 8 BglII A'GATC_T Cuts at: 0 818 1046 1296 1869 Size: 818 228 250 573 Fragments arranged by size: 818 573 250 228 BlpI GC'TnA_GC Cuts at: 0 800 1796 1869 Size: 800 996 73 Fragments arranged by size: 996 800 73 Bme1580I G_kGCm'C Cuts at: 0 329 1151 1277 1869 Size: 329 822 126 592 Fragments arranged by size: 822 592 329 126 BmgBI CAC'GTC Cuts at: 0 116 357 1869 Size: 116 241 1512 Fragments arranged by size: 1512 241 116 BsaAI yAC'GTr Cuts at: 0 67 1869 Size: 67 1802 BsaBI GATnn'nnATC Cuts at: 0 598 1582 1869 Size: 598 984 287 Fragments arranged by size: 984 598 287 BsaWI w'CCGG_w Cuts at: 0 1409 1869 Size: 1409 460 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 655 685 1261 1291 1869 Size: 655 30 576 30 578 Fragments arranged by size: 655 578 576 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 679 1869 Size: 679 1190 BseYI C'CCAG_C Cuts at: 0 456 910 919 981 1522 1869 Size: 456 454 9 62 541 347 Fragments arranged by size: 541 456 454 347 62 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1519 1742 1869 Size: 1519 223 127 Fragments arranged by size: 1519 223 127 BsiHKAI G_wGCw'C Cuts at: 0 218 1064 1869 Size: 218 846 805 Fragments arranged by size: 846 805 218 Bsp1286I G_dGCh'C Cuts at: 0 103 218 329 484 1064 1151 1277 1869 Size: 103 115 111 155 580 87 126 592 Fragments arranged by size: 592 580 155 126 115 111 103 87 BspEI T'CCGG_A Cuts at: 0 1409 1869 Size: 1409 460 BsrDI GCAATG_nn' Cuts at: 0 713 890 1088 1832 1869 Size: 713 177 198 744 37 Fragments arranged by size: 744 713 198 177 37 BsrFI r'CCGG_y Cuts at: 0 412 1869 Size: 412 1457 BssSI C'ACGA_G Cuts at: 0 1537 1646 1869 Size: 1537 109 223 Fragments arranged by size: 1537 223 109 BstAPI GCAn_nnn'nTGC Cuts at: 0 33 1869 Size: 33 1836 BstEII G'GTnAC_C Cuts at: 0 1506 1527 1869 Size: 1506 21 342 Fragments arranged by size: 1506 342 21 BstXI CCAn_nnnn'nTGG Cuts at: 0 645 795 1869 Size: 645 150 1074 Fragments arranged by size: 1074 645 150 BstYI r'GATC_y Cuts at: 0 818 830 848 1046 1296 1664 1869 Size: 818 12 18 198 250 368 205 Fragments arranged by size: 818 368 250 205 198 18 12 Bsu36I CC'TnA_GG Cuts at: 0 298 1869 Size: 298 1571 BtgI C'CryG_G Cuts at: 0 497 1673 1869 Size: 497 1176 196 Fragments arranged by size: 1176 497 196 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 990 1098 1869 Size: 990 108 771 Fragments arranged by size: 990 771 108 BtsI GCAGTG_nn' Cuts at: 0 89 1245 1376 1869 Size: 89 1156 131 493 Fragments arranged by size: 1156 493 131 89 DraIII CAC_nnn'GTG Cuts at: 0 660 1869 Size: 660 1209 DrdI GACnn_nn'nnGTC Cuts at: 0 1462 1869 Size: 1462 407 EaeI y'GGCC_r Cuts at: 0 53 105 275 359 536 684 974 1205 Size: 53 52 170 84 177 148 290 231 Cuts at: 1205 1547 1869 Size: 342 322 Fragments arranged by size: 342 322 290 231 177 170 148 84 53 52 EarI CTCTTCn'nnn_ Cuts at: 0 1483 1869 Size: 1483 386 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 298 1869 Size: 298 1571 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 281 1507 1585 1825 1869 Size: 281 1226 78 240 44 Fragments arranged by size: 1226 281 240 78 44 EcoNI CCTnn'n_nnAGG Cuts at: 0 277 376 1537 1869 Size: 277 99 1161 332 Fragments arranged by size: 1161 332 277 99 EcoO109I rG'GnC_Cy Cuts at: 0 183 381 1274 1851 1869 Size: 183 198 893 577 18 Fragments arranged by size: 893 577 198 183 18 HaeII r_GCGC'y Cuts at: 0 131 1646 1869 Size: 131 1515 223 Fragments arranged by size: 1515 223 131 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 748 780 865 897 1261 1293 1699 1731 Size: 748 32 85 32 364 32 406 32 Cuts at: 1731 1869 Size: 138 Fragments arranged by size: 748 406 364 138 85 32 32 32 32 HincII GTy'rAC Cuts at: 0 1767 1869 Size: 1767 102 HindIII A'AGCT_T Cuts at: 0 344 1869 Size: 344 1525 HpaI GTT'AAC Cuts at: 0 1767 1869 Size: 1767 102 KpnI G_GTAC'C Cuts at: 0 1777 1869 Size: 1777 92 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 338 1446 1869 Size: 338 1108 423 Fragments arranged by size: 1108 423 338 MscI TGG'CCA Cuts at: 0 107 361 538 686 976 1869 Size: 107 254 177 148 290 893 Fragments arranged by size: 893 290 254 177 148 107 MslI CAynn'nnrTG Cuts at: 0 355 643 904 992 1117 1588 1688 1869 Size: 355 288 261 88 125 471 100 181 Fragments arranged by size: 471 355 288 261 181 125 100 88 MspA1I CmG'CkG Cuts at: 0 582 1552 1795 1869 Size: 582 970 243 74 Fragments arranged by size: 970 582 243 74 NcoI C'CATG_G Cuts at: 0 497 1869 Size: 497 1372 NspI r_CATG'y Cuts at: 0 637 909 1869 Size: 637 272 960 Fragments arranged by size: 960 637 272 PasI CC'CwG_GG Cuts at: 0 372 1869 Size: 372 1497 PflMI CCAn_nnn'nTGG Cuts at: 0 1348 1783 1869 Size: 1348 435 86 Fragments arranged by size: 1348 435 86 PfoI T'CCnGG_A Cuts at: 0 812 1869 Size: 812 1057 PpuMI rG'GwC_Cy Cuts at: 0 183 1869 Size: 183 1686 PspOMI G'GGCC_C Cuts at: 0 1273 1869 Size: 1273 596 PstI C_TGCA'G Cuts at: 0 38 127 587 1159 1869 Size: 38 89 460 572 710 Fragments arranged by size: 710 572 460 89 38 PvuII CAG'CTG Cuts at: 0 1795 1869 Size: 1795 74 RsrII CG'GwC_CG Cuts at: 0 1068 1869 Size: 1068 801 SapI GCTCTTCn'nnn_ Cuts at: 0 1483 1869 Size: 1483 386 SexAI A'CCwGG_T Cuts at: 0 1502 1869 Size: 1502 367 SfcI C'TryA_G Cuts at: 0 34 123 583 822 1155 1358 1869 Size: 34 89 460 239 333 203 511 Fragments arranged by size: 511 460 333 239 203 89 34 SnaBI TAC'GTA Cuts at: 0 67 1869 Size: 67 1802 SphI G_CATG'C Cuts at: 0 637 909 1869 Size: 637 272 960 Fragments arranged by size: 960 637 272 StuI AGG'CCT Cuts at: 0 40 622 679 958 1543 1869 Size: 40 582 57 279 585 326 Fragments arranged by size: 585 582 326 279 57 40 StyI C'CwwG_G Cuts at: 0 221 497 1475 1544 1655 1862 1869 Size: 221 276 978 69 111 207 7 Fragments arranged by size: 978 276 221 207 111 69 7 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1301 1768 1869 Size: 1301 467 101 Fragments arranged by size: 1301 467 101 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 644 655 676 687 1869 Size: 644 11 21 11 1182 Fragments arranged by size: 1182 644 21 11 11 XbaI T'CTAG_A Cuts at: 0 543 1869 Size: 543 1326 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 369 1614 1725 1869 Size: 369 1245 111 144 Fragments arranged by size: 1245 369 144 111 Enzymes that do cut: Acc65I AcuI AfeI AleI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbsI BcgI BglII BlpI Bme1580I BmgBI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HindIII HpaI KpnI MmeI MscI MslI MspA1I NcoI NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII RsrII SapI SexAI SfcI SnaBI SphI StuI StyI TaqII TstI XbaI XcmI Enzymes that do not cut: AarI AatII AccI AclI AflII AflIII AgeI AhdI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BciVI BclI BfrBI BglI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsiEI BsiWI BsmI BsmBI BspHI BspMI BsrBI BsrGI BssHII BstBI BstZ17I ClaI CspCI DraI EagI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI KasI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI SacI SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SpeI SrfI SspI SwaI TatI Tth111I XhoI XmaI XmnI ZraI