################################## # NO CUT ENZYMES ( 143 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AflIII AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BamHI BbeI BbrPI BbvI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmFI BsmI BspDI BspEI BspHI BspLU11I BspMI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstF5I BstXI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HaeII HgaI HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MseI MspA1I MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI TfiI Tru9I TseI Tth111I TthHB8I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AatII 568 GACGTC GACGT^C AccII 123 CGCG CG^CG AcsI 166 GAATTT [GA]^AATT[CT] AcyI 568 GACGTC G[GA]^CG[CT]C AfaI 130 GTAC GT^AC AhdI 103 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 473 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 352 CCTGAGG CC^T[ACGT]AGG ApoI 166 GAATTT [GA]^AATT[CT] AspEI 103 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 761 CCCGGG C^[CT]CG[GA]G BalI 750 TGGCCA TGG^CCA BanI 718 GGCACC G^G[CT][GA]CC BbsI 10 GAAGAC GAAGAC(2/6) BbvCI 557 CCTCAGC CCTCAGC(-5/-2) BfaI 700 CTAG C^TAG BpmI 226 CTGGAG CTGGAG(16/14) BpuAI 10 GAAGAC GAAGAC(2/6) BsaHI 568 GACGTC G[GA]^CG[CT]C BsaI 211 GAGACC GGTCTC(1/5) BsoBI 761 CCCGGG C^[CT]CG[GA]G Bsp1407I 129 TGTACA T^GTACA BsrBI 610 CCGCTC CCGCTC(-3/-3) BsrGI 129 TGTACA T^GTACA BstEII 357 GGTGACC G^GT[ACGT]ACC BstPI 357 GGTGACC G^GT[ACGT]ACC BstUI 123 CGCG CG^CG BstYI 195 GGATCT [GA]^GATC[CT] Bsu36I 352 CCTGAGG CC^T[ACGT]AGG CfoI 3 GCGC GCG^C Eam1105I 103 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 499 CTCTTC CTCTTC(1/4) Eco81I 352 CCTGAGG CC^T[ACGT]AGG EcoO65I 357 GGTGACC G^GT[ACGT]ACC FauI 266 GCGGG CCCGC(4/6) HapII 762 CCGG C^CGG HhaI 3 GCGC GCG^C Hin1I 568 GACGTC G[GA]^CG[CT]C HinP1I 3 GCGC G^CGC HincII 111 GTCAAC GT[CT]^[GA]AC HindII 111 GTCAAC GT[CT]^[GA]AC HinfI 72 GAGTC G^A[ACGT]TC HpaII 762 CCGG C^CGG HphI 357 GGTGA GGTGA(8/7) Ksp632I 499 CTCTTC CTCTTC(1/4) MaeI 700 CTAG C^TAG MflI 195 GGATCT [GA]^GATC[CT] MluNI 750 TGGCCA TGG^CCA MscI 750 TGGCCA TGG^CCA MspI 762 CCGG C^CGG MvnI 123 CGCG CG^CG PleI 72 GAGTC GAGTC(4/5) PstI 579 CTGCAG CTGCA^G RsaI 130 GTAC GT^AC SfcI 579 CTGCAG C^T[GA][CT]AG SmaI 761 CCCGGG CCC^GGG SspBI 129 TGTACA T^GTACA Tsp509I 167 AATT ^AATT XcmI 371 CCAAGGAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 195 GGATCT [GA]^GATC[CT] XmaCI 761 CCCGGG C^CCGGG XmaI 761 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### ApaI 764 GGGCCC GGGCC^C 772 GGGCCC AvaII 102 GGACC G^G[AT]CC 603 GGACC BcnI 761 CCCGG CC^[GC]GG 762 CCGGG BglI 91 GCCATCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 698 GCCTAGTGGGC BsrDI 279 CATTGC GCAATG(2/0) 461 GCAATG DraII 771 GGGGCCC [GA]G^G[ACGT]CC[CT] 772 GGGCCCC EaeI 237 TGGCCG [CT]^GGCC[GA] 750 TGGCCA EcoO109I 771 GGGGCCC [GA]G^G[ACGT]CC[CT] 772 GGGCCCC EcoT14I 56 CCTTGG C^C[AT][AT]GG 371 CCAAGG MaeII 446 ACGT A^CGT 569 ACGT MslI 452 CATGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 708 CATCATCGTG NciI 761 CCCGG CC^[GC]GG 762 CCGGG SmlI 42 CTTGAG C^T[CT][GA]AG 616 CTCAAG StyI 56 CCTTGG C^C[AT][AT]GG 371 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AciI AluI AlwI AspHI BanII BmyI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI HaeIII ItaI MaeIII MboI MboII MnlI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI Tsp45I TspRI