################################## # NO CUT ENZYMES ( 143 enzymes) # ################################## AatII Acc65I AccI AccIII AcyI AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BglI BglII BlnI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmFI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BssSI Bst1107I BstAPI BstBI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII Hin1I HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StyI SwaI Tsp45I TspRI Tth111I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AclI 369 AACGTT AA^CGTT AflII 729 CTTAAG C^TTAAG AlwI 747 GGATC GGATC(4/5) ApaI 394 GGGCCC GGGCC^C Asp700I 859 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanII 394 GGGCCC G[GA]GC[CT]^C BbsI 765 GAAGAC GAAGAC(2/6) BfrI 729 CTTAAG C^TTAAG BlpI 12 GCTGAGC GC^T[ACGT]AGC BmyI 394 GGGCCC G[AGT]GC[ACT]^C BpmI 644 CTGGAG CTGGAG(16/14) Bpu1102I 12 GCTGAGC GC^T[ACGT]AGC BpuAI 765 GAAGAC GAAGAC(2/6) BsaAI 103 TACGTG [CT]AC^GT[GA] BsgI 283 CTGCAC GTGCAG(16/14) BsmI 724 GAATGC GAATGC(1/-1) Bsp1286I 394 GGGCCC G[AGT]GC[ACT]^C Bsp1407I 480 TGTACA T^GTACA BsrDI 249 GCAATG GCAATG(2/0) BsrGI 480 TGTACA T^GTACA BssHII 30 GCGCGC G^CGCGC BstEII 73 GGTTACC G^GT[ACGT]ACC BstPI 73 GGTTACC G^GT[ACGT]ACC BstYI 747 GGATCT [GA]^GATC[CT] CelII 12 GCTGAGC GC^T[ACGT]AGC DraII 393 AGGGCCC [GA]G^G[ACGT]CC[CT] EarI 580 GAAGAG CTCTTC(1/4) EcoO109I 393 AGGGCCC [GA]G^G[ACGT]CC[CT] EcoO65I 73 GGTTACC G^GT[ACGT]ACC EcoRI 601 GAATTC G^AATTC FauI 70 GCGGG CCCGC(4/6) HapII 59 CCGG C^CGG HgaI 208 GACGC GACGC(5/10) HincII 372 GTTGAC GT[CT]^[GA]AC HindII 372 GTTGAC GT[CT]^[GA]AC HpaII 59 CCGG C^CGG Ksp632I 580 GAAGAG CTCTTC(1/4) MflI 747 GGATCT [GA]^GATC[CT] MseI 730 TTAA T^TAA MslI 165 CATCTCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 59 CCGG C^CGG PleI 375 GACTC GAGTC(4/5) Psp1406I 369 AACGTT AA^CGTT PvuII 272 CAGCTG CAG^CTG ScaI 460 AGTACT AGT^ACT SmaI 58 CCCGGG CCC^GGG SmlI 729 CTTAAG C^T[CT][GA]AG SspBI 480 TGTACA T^GTACA StuI 525 AGGCCT AGG^CCT Tru9I 730 TTAA T^TAA XhoII 747 GGATCT [GA]^GATC[CT] XmaCI 58 CCCGGG C^CCGGG XmaI 58 CCCGGG C^CCGGG XmnI 859 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AcsI 601 GAATTC [GA]^AATT[CT] 708 AAATTT ApoI 601 GAATTC [GA]^AATT[CT] 708 AAATTT AvaI 58 CCCGGG C^[CT]CG[GA]G 87 CTCGGG AvaII 198 GGACC G^G[AT]CC 608 GGTCC BcnI 58 CCCGG CC^[GC]GG 59 CCGGG BfaI 464 CTAG C^TAG 803 CTAG BsmAI 554 GAGAC GTCTC(1/5) 583 GAGAC BsoBI 58 CCCGGG C^[CT]CG[GA]G 87 CTCGGG BstF5I 294 CATCC GGATG(2/0) 743 GGATG FokI 294 CATCC GGATG(9/13) 743 GGATG MaeI 464 CTAG C^TAG 803 CTAG MaeII 104 ACGT A^CGT 370 ACGT MaeIII 74 GTTAC ^GT[ACGT]AC 101 GTTAC NciI 58 CCCGG CC^[GC]GG 59 CCGGG PstI 136 CTGCAG CTGCA^G 239 CTGCAG SfcI 136 CTGCAG C^T[GA][CT]AG 239 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AccII AciI AfaI AluI AlwNI BbvI BsaJI BsiYI BslI BsrI BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII EcoRII Fnu4HI HaeIII HhaI HinP1I HinfI HphI ItaI MboI MboII MnlI MspA1I MvaI MvnI MwoI NdeII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TfiI TseI Tsp509I TthHB8I