(Circular) (Six-base) MAPSORT of: pFN21AE6334.seq Check: 2692 from: 1 to: 5653 pFN21AE6334 5653 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3463 278 Size: 53 83 186 936 1927 2468 Fragments arranged by size: 2468 1927 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2996 1937 Size: 1059 4594 Acc65I G'GTAC_C Cuts at: 2974 2974 Size: 5653 AclI AA'CG_TT Cuts at: 1563 2375 3780 4153 1563 Size: 812 1405 373 3063 Fragments arranged by size: 3063 1405 812 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2565 3716 5100 5368 1632 Size: 933 1151 1384 268 1917 Fragments arranged by size: 1917 1384 1151 933 268 AflII C'TTAA_G Cuts at: 820 1017 2734 820 Size: 197 1717 3739 Fragments arranged by size: 3739 1717 197 AflIII A'CryG_T Cuts at: 1203 4552 5529 1203 Size: 3349 977 1327 Fragments arranged by size: 3349 1327 977 AgeI A'CCGG_T Cuts at: 4484 4484 Size: 5653 AhdI GACnn_n'nnGTC Cuts at: 4382 4382 Size: 5653 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5653 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2192 1184 Size: 1008 4645 AlwNI CAG_nnn'CTG Cuts at: 2017 2450 2543 3396 4968 2017 Size: 433 93 853 1572 2702 Fragments arranged by size: 2702 1572 853 433 93 ApaI G_GGCC'C Cuts at: 2403 2403 Size: 5653 ApaLI G'TGCA_C Cuts at: 3710 4866 3710 Size: 1156 4497 ApoI r'AATT_y Cuts at: 1493 2606 2713 2962 3188 3340 5449 1493 Size: 1113 107 249 226 152 2109 1697 Fragments arranged by size: 2109 1697 1113 249 226 152 107 AseI AT'TA_AT Cuts at: 160 1032 4207 160 Size: 872 3175 1606 Fragments arranged by size: 3175 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5653 AvaI C'yCGr_G Cuts at: 1870 1943 2063 2092 2978 1870 Size: 73 120 29 886 4545 Fragments arranged by size: 4545 886 120 73 29 BamHI G'GATC_C Cuts at: 2983 3350 2983 Size: 367 5286 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2974 4429 618 Size: 325 217 627 1187 1455 1842 Fragments arranged by size: 1842 1455 1187 627 325 217 BanII G_rGCy'C Cuts at: 1391 2403 2972 1391 Size: 1012 569 4072 Fragments arranged by size: 4072 1012 569 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2777 928 Size: 971 878 3804 Fragments arranged by size: 3804 971 878 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3844 3878 1903 Size: 34 50 34 1823 34 3678 Fragments arranged by size: 3678 1823 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3545 4755 3545 Size: 1210 4443 BclI T'GATC_A Cuts at: 1548 1548 Size: 5653 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4264 136 Size: 107 122 71 631 605 2592 1525 Fragments arranged by size: 2592 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5648 5648 Size: 5653 BlpI GC'TnA_GC Cuts at: 2018 3070 2018 Size: 1052 4601 Bme1580I G_kGCm'C Cuts at: 1790 2403 3714 4870 1790 Size: 613 1311 1156 2573 Fragments arranged by size: 2573 1311 1156 613 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5653 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3384 4342 442 Size: 416 53 83 399 24 1967 958 1753 Fragments arranged by size: 1967 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5653 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2670 4313 1239 Size: 1431 1643 2579 Fragments arranged by size: 2579 1643 1431 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3778 4643 4941 3778 Size: 865 298 4490 Fragments arranged by size: 4490 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4316 882 Size: 617 2817 2219 Fragments arranged by size: 2817 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2110 3444 493 Size: 711 906 1334 2702 Fragments arranged by size: 2702 1334 906 711 BsaBI GATnn'nnATC Cuts at: 5647 5647 Size: 5653 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3460 3842 4477 275 Size: 53 83 186 936 1927 382 635 1451 Fragments arranged by size: 1927 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4086 4484 4758 4905 5316 4086 Size: 398 274 147 411 4423 Fragments arranged by size: 4423 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5428 Fragments arranged by size: 5428 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5653 BseYI C'CCAG_C Cuts at: 2022 2085 2402 4856 5352 2022 Size: 63 317 2454 496 2323 Fragments arranged by size: 2454 2323 496 317 63 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2273 1163 Size: 573 537 4543 Fragments arranged by size: 4543 573 537 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3103 3864 4892 1540 Size: 275 186 1102 761 1028 2301 Fragments arranged by size: 2301 1102 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3714 3799 4870 3714 Size: 85 1071 4497 Fragments arranged by size: 4497 1071 85 BsmI GAATG_Cn' Cuts at: 2735 3164 3257 2735 Size: 429 93 5131 Fragments arranged by size: 5131 429 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2403 2972 3714 3799 4870 1391 Size: 399 613 569 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 613 569 399 85 BspEI T'CCGG_A Cuts at: 5316 5316 Size: 5653 BspHI T'CATG_A Cuts at: 3542 5272 3542 Size: 1730 3923 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3008 844 Size: 2164 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3540 1394 Size: 429 1717 3507 Fragments arranged by size: 3507 1717 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2261 4148 4322 65 Size: 1183 1013 1887 174 1396 Fragments arranged by size: 1887 1396 1183 1013 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4297 4484 1700 Size: 110 2487 187 2869 Fragments arranged by size: 2869 2487 187 110 BsrGI T'GTAC_A Cuts at: 96 2485 96 Size: 2389 3264 BssHII G'CGCG_C Cuts at: 1925 2035 1925 Size: 110 5543 BssSI C'ACGA_G Cuts at: 3713 4725 3713 Size: 1012 4641 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5653 BstBI TT'CG_AA Cuts at: 4457 4457 Size: 5653 BstEII G'GTnAC_C Cuts at: 1184 2078 1184 Size: 894 4759 BstYI r'GATC_y Cuts at: 1972 2752 2983 3350 3735 3752 5204 5290 5302 Size: 780 231 367 385 17 1452 86 12 Cuts at: 5302 5648 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 780 385 367 346 231 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5101 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2054 487 Size: 683 56 828 4086 Fragments arranged by size: 4086 828 683 56 BtsI GCAGTG_nn' Cuts at: 3169 3963 3983 3169 Size: 794 20 4839 Fragments arranged by size: 4839 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5653 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5618 DraI TTT'AAA Cuts at: 2957 2957 Size: 5653 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4660 809 Size: 507 3344 1802 Fragments arranged by size: 3344 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3100 3989 5618 Size: 54 1425 50 275 18 1270 889 1629 Cuts at: 5618 8 Size: 43 Fragments arranged by size: 1629 1425 1270 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3100 1537 Size: 275 1288 4090 Fragments arranged by size: 4090 1288 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2580 3583 1353 Size: 1227 1003 3423 Fragments arranged by size: 3423 1227 1003 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2109 4240 4614 4760 387 Size: 1064 138 520 2131 374 146 1280 Fragments arranged by size: 2131 1280 1064 520 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2565 2670 3716 4313 5100 5368 1239 Size: 393 933 105 1046 597 787 268 1524 Fragments arranged by size: 1524 1046 933 787 597 393 268 105 EcoO109I rG'GnC_Cy Cuts at: 2399 2399 Size: 5653 EcoRI G'AATT_C Cuts at: 2606 2962 3340 2606 Size: 356 378 4919 Fragments arranged by size: 4919 378 356 FspI TGC'GCA Cuts at: 4159 4159 Size: 5653 HaeII r_GCGC'y Cuts at: 1678 4800 1678 Size: 3122 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4294 4326 4368 4400 1373 Size: 32 135 32 2722 32 42 32 2626 Fragments arranged by size: 2722 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2379 2997 3243 669 Size: 988 281 441 618 246 3079 Fragments arranged by size: 3079 988 618 441 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5653 HpaI GTT'AAC Cuts at: 3243 3243 Size: 5653 KpnI G_GTAC'C Cuts at: 2978 2978 Size: 5653 MfeI C'AATT_G Cuts at: 3252 3252 Size: 5653 MluI A'CGCG_T Cuts at: 5529 5529 Size: 5653 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2107 4767 4951 1305 Size: 802 2660 184 2007 Fragments arranged by size: 2660 2007 802 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3831 Fragments arranged by size: 3831 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2174 3611 3970 4129 518 Size: 648 1008 1437 359 159 2042 Fragments arranged by size: 2042 1437 1008 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2017 2031 2085 2279 2567 3069 3746 4894 Size: 411 14 54 194 288 502 677 1148 Cuts at: 4894 5139 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 502 411 288 245 194 54 14 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5101 NdeI CA'TA_TG Cuts at: 387 387 Size: 5653 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5653 NotI GC'GGCC_GC Cuts at: 3100 3100 Size: 5653 NspI r_CATG'y Cuts at: 1132 1229 3011 4556 1132 Size: 97 1782 1545 2229 Fragments arranged by size: 2229 1782 1545 97 PasI CC'CwG_GG Cuts at: 1348 2335 1348 Size: 987 4666 PciI A'CATG_T Cuts at: 4552 4552 Size: 5653 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5653 PfoI T'CCnGG_A Cuts at: 2524 2647 2524 Size: 123 5530 PmeI GTTT'AAAC Cuts at: 2957 2957 Size: 5653 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3722 3754 5235 5267 3722 Size: 32 1481 32 4108 Fragments arranged by size: 4108 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5653 PsiI TTA'TAA Cuts at: 2898 2931 3223 2898 Size: 33 292 5328 Fragments arranged by size: 5328 292 33 PspOMI G'GGCC_C Cuts at: 2399 2399 Size: 5653 PstI C_TGCA'G Cuts at: 830 2145 2248 3005 830 Size: 1315 103 757 3478 Fragments arranged by size: 3478 1315 757 103 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5653 PvuII CAG'CTG Cuts at: 2279 2279 Size: 5653 SalI G'TCGA_C Cuts at: 1936 2995 1936 Size: 1059 4594 SbfI CC_TGCA'GG Cuts at: 3005 3005 Size: 5653 ScaI AGT'ACT Cuts at: 2467 3901 5613 2467 Size: 1434 1712 2507 Fragments arranged by size: 2507 1712 1434 SfcI C'TryA_G Cuts at: 826 1045 2141 2244 3001 4136 4817 5008 826 Size: 219 1096 103 757 1135 681 191 1471 Fragments arranged by size: 1471 1135 1096 757 681 219 191 103 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5653 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5653 SmaI CCC'GGG Cuts at: 1872 2065 2980 1872 Size: 193 915 4545 Fragments arranged by size: 4545 915 193 SmlI C'TyrA_G Cuts at: 820 1017 1943 2734 3757 4658 4920 820 Size: 197 926 791 1023 901 262 1553 Fragments arranged by size: 1553 1023 926 901 791 262 197 SnaBI TAC'GTA Cuts at: 493 3444 493 Size: 2951 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5653 SphI G_CATG'C Cuts at: 1132 3011 1132 Size: 1879 3774 SspI AAT'ATT Cuts at: 5 52 3577 5 Size: 47 3525 2081 Fragments arranged by size: 3525 2081 47 StuI AGG'CCT Cuts at: 1517 2532 1517 Size: 1015 4638 StyI C'CwwG_G Cuts at: 513 1065 3092 513 Size: 552 2027 3074 Fragments arranged by size: 3074 2027 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3680 3697 3850 4035 4480 325 Size: 1255 2100 17 153 185 445 1498 Fragments arranged by size: 2100 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2248 2465 2485 Size: 274 80 33 51 1443 271 217 20 Cuts at: 2485 3899 5611 96 Size: 1414 1712 138 Fragments arranged by size: 1712 1443 1414 274 271 217 138 80 51 33 20 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5613 5645 5613 Size: 32 5621 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5653 XbaI T'CTAG_A Cuts at: 2989 2989 Size: 5653 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5653 XmaI C'CCGG_G Cuts at: 1870 2063 2978 1870 Size: 193 915 4545 Fragments arranged by size: 4545 915 193 XmnI GAAnn'nnTTC Cuts at: 2868 3782 2868 Size: 914 4739 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3461 276 Size: 53 83 186 936 1927 2468 Fragments arranged by size: 2468 1927 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI