################################## # NO CUT ENZYMES ( 157 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AflIII AgeI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BalI BamHI BbrPI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstF5I BstUI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT14I EcoT22I FbaI FokI FseI FspI HgaI HincII HindII HindIII HpaI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MunI MvnI NciI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfuI SmaI SnaBI SpeI SphI Sse8387I StuI StyI SwaI Tsp45I Tth111I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AcyI 24 GGCGCC G[GA]^CG[CT]C AfaI 742 GTAC GT^AC AhdI 674 GACAATACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 633 GATCC GGATC(4/5) AspEI 674 GACAATACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 487 GAGCAC G[AT]GC[AT]^C AvaI 228 CTCGGG C^[CT]CG[GA]G BanI 24 GGCGCC G^G[CT][GA]CC BanII 5 GAGCCC G[GA]GC[CT]^C BbeI 24 GGCGCC GGCGC^C BbvCI 703 GCTGAGG CCTCAGC(-5/-2) BfaI 212 CTAG C^TAG BsaHI 24 GGCGCC G[GA]^CG[CT]C BsaJI 227 CCTCGG C^C[ACGT][ACGT]GG BsiHKAI 487 GAGCAC G[AT]GC[AT]^C BsmFI 125 GGGAC GGGAC(10/14) BsoBI 228 CTCGGG C^[CT]CG[GA]G Bsp1407I 741 TGTACA T^GTACA BsrGI 741 TGTACA T^GTACA BstEII 150 GGTTACC G^GT[ACGT]ACC BstPI 150 GGTTACC G^GT[ACGT]ACC Cac8I 21 GCCGGC GC[ACGT]^[ACGT]GC CfoI 25 GCGC GCG^C DpnII 633 GATC ^GATC Eam1105I 674 GACAATACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 253 GAAGAG CTCTTC(1/4) EcoO65I 150 GGTTACC G^GT[ACGT]ACC FauI 9 CCCGC CCCGC(4/6) HaeII 24 GGCGCC [GA]GCGC^[CT] HaeIII 61 GGCC GG^CC HhaI 25 GCGC GCG^C Hin1I 24 GGCGCC G[GA]^CG[CT]C HinP1I 25 GCGC G^CGC HinfI 298 GATTC G^A[ACGT]TC HphI 532 GGTGA GGTGA(8/7) KasI 24 GGCGCC G^GCGCC Ksp632I 253 GAAGAG CTCTTC(1/4) MaeI 212 CTAG C^TAG MaeII 680 ACGT A^CGT MboI 633 GATC ^GATC MseI 323 TTAA T^TAA MslI 506 CATGCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 21 GCCGGC GCC^GGC NarI 24 GGCGCC GG^CGCC NdeII 633 GATC ^GATC NgoMIV 21 GCCGGC G^CCGGC PflMI 115 CCATTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 641 CAGCTG CAG^CTG RsaI 742 GTAC GT^AC Sau3AI 633 GATC ^GATC SfcI 749 CTATAG C^T[GA][CT]AG SfoI 24 GGCGCC GGC^GCC SmlI 156 CTCAAG C^T[CT][GA]AG SspBI 741 TGTACA T^GTACA SspI 524 AATATT AAT^ATT TfiI 298 GATTC G^A[AT]TC Tru9I 323 TTAA T^TAA TspRI 132 CAGTG CA[GC]TG[ACGT][ACGT]^ Van91I 115 CCATTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AciI 10 CCGC CCGC(-3/-1) 19 CCGC AcsI 445 GAATTT [GA]^AATT[CT] 453 AAATTT ApoI 445 GAATTT [GA]^AATT[CT] 453 AAATTT AvaII 81 GGACC G^G[AT]CC 126 GGACC BbsI 671 GAAGAC GAAGAC(2/6) 171 GTCTTC BmyI 5 GAGCCC G[AGT]GC[ACT]^C 487 GAGCAC BpuAI 671 GAAGAC GAAGAC(2/6) 171 GTCTTC BsmI 472 GAATGC GAATGC(1/-1) 461 GCATTC Bsp1286I 5 GAGCCC G[AGT]GC[ACT]^C 487 GAGCAC BsrFI 21 GCCGGC [GA]^CCGG[CT] 27 GCCGGT BssKI 280 CCTGG ^CC[ACGT]GG 492 CCTGG BstNI 280 CCTGG CC^[AT]GG 492 CCTGG Cfr10I 21 GCCGGC [GA]^CCGG[CT] 27 GCCGGT Cfr13I 81 GGACC G^G[ACGT]CC 126 GGACC EcoRII 280 CCTGG ^CC[AT]GG 492 CCTGG HapII 22 CCGG C^CGG 28 CCGG HpaII 22 CCGG C^CGG 28 CCGG MaeIII 151 GTTAC ^GT[ACGT]AC 502 GTAAC MspA1I 10 CCGCTG C[AC]G^C[GT]G 641 CAGCTG MspI 22 CCGG C^CGG 28 CCGG MvaI 280 CCTGG CC^[AT]GG 492 CCTGG NspI 505 ACATGC [GA]CATG^[CT] 744 ACATGC Sau96I 81 GGACC G^G[ACGT]CC 126 GGACC ScrFI 280 CCTGG CC^[ACGT]GG 492 CCTGG SgrAI 20 CGCCGGCG C[GA]^CCGG[CT]G 26 CGCCGGTG TaqI 293 TCGA T^CGA 723 TCGA TthHB8I 293 TCGA T^CGA 723 TCGA XcmI 63 CCATTATGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 129 CCACAGTGCTGATGG ######################################### # 3 OR MORE CUT ENZYMES ( 14 enzymes) # ######################################### AluI BbvI BsiYI BslI DdeI Fnu4HI ItaI MboII MnlI MwoI NlaIII NlaIV TseI Tsp509I