(Circular) (Six-base) MAPSORT of: pFN21AB6990.seq Check: 2972 from: 1 to: 5461 pFN21AB6990 5461 bp With 182 enzymes: SgfI * August 16, 2010 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3271 278 Size: 53 83 186 936 1735 2468 Fragments arranged by size: 2468 1735 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2804 1937 Size: 867 4594 Acc65I G'GTAC_C Cuts at: 2782 2782 Size: 5461 AclI AA'CG_TT Cuts at: 1563 3588 3961 1563 Size: 2025 373 3063 Fragments arranged by size: 3063 2025 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2163 2349 3524 4908 5176 1632 Size: 531 186 1175 1384 268 1917 Fragments arranged by size: 1917 1384 1175 531 268 186 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5264 AflIII A'CryG_T Cuts at: 1203 4360 5337 1203 Size: 3157 977 1327 Fragments arranged by size: 3157 1327 977 AgeI A'CCGG_T Cuts at: 4292 4292 Size: 5461 AhdI GACnn_n'nnGTC Cuts at: 2684 4190 2684 Size: 1506 3955 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5461 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2527 1184 Size: 1343 4118 AlwNI CAG_nnn'CTG Cuts at: 3204 4776 3204 Size: 1572 3889 ApaLI G'TGCA_C Cuts at: 3518 4674 3518 Size: 1156 4305 ApoI r'AATT_y Cuts at: 1493 2450 2458 2770 2996 3148 5257 1493 Size: 957 8 312 226 152 2109 1697 Fragments arranged by size: 2109 1697 957 312 226 152 8 AseI AT'TA_AT Cuts at: 160 1032 4015 160 Size: 872 2983 1606 Fragments arranged by size: 2983 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5461 AvaI C'yCGr_G Cuts at: 1870 1943 2233 2786 1870 Size: 73 290 553 4545 Fragments arranged by size: 4545 553 290 73 BamHI G'GATC_C Cuts at: 2791 3158 2791 Size: 367 5094 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2029 2782 4237 618 Size: 325 217 627 242 753 1455 1842 Fragments arranged by size: 1842 1455 753 627 325 242 217 BanII G_rGCy'C Cuts at: 1391 2014 2780 1391 Size: 623 766 4072 Fragments arranged by size: 4072 766 623 BbeI G_GCGC'C Cuts at: 2033 2033 Size: 5461 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2169 2683 928 Size: 971 270 514 3706 Fragments arranged by size: 3706 971 514 270 BbvCI CC'TCA_GC Cuts at: 2709 2709 Size: 5461 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3652 3686 1903 Size: 34 1715 34 3678 Fragments arranged by size: 3678 1715 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3353 4563 3353 Size: 1210 4251 BclI T'GATC_A Cuts at: 1548 1548 Size: 5461 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4072 136 Size: 107 122 71 631 605 2400 1525 Fragments arranged by size: 2400 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5456 5456 Size: 5461 BlpI GC'TnA_GC Cuts at: 2878 2878 Size: 5461 Bme1580I G_kGCm'C Cuts at: 1790 3522 4678 1790 Size: 1732 1156 2573 Fragments arranged by size: 2573 1732 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5461 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3192 4150 442 Size: 416 53 83 399 24 1775 958 1753 Fragments arranged by size: 1775 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5461 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2176 2176 Size: 5461 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4121 1239 Size: 2882 2579 Bpu10I CC'TnA_GC Cuts at: 2062 2709 2062 Size: 647 4814 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2146 3586 4451 4749 2146 Size: 1440 865 298 2858 Fragments arranged by size: 2858 1440 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4124 882 Size: 617 2625 2219 Fragments arranged by size: 2625 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3252 493 Size: 711 2048 2702 Fragments arranged by size: 2702 2048 711 BsaBI GATnn'nnATC Cuts at: 5455 5455 Size: 5461 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2030 3268 3650 4285 Size: 53 83 186 936 497 1238 382 635 Cuts at: 4285 275 Size: 1451 Fragments arranged by size: 1451 1238 936 635 497 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3894 4292 4566 4713 5124 3894 Size: 398 274 147 411 4231 Fragments arranged by size: 4231 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5236 Fragments arranged by size: 5236 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5461 BseYI C'CCAG_C Cuts at: 4664 5160 4664 Size: 496 4965 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4888 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2911 3672 4700 1540 Size: 275 186 910 761 1028 2301 Fragments arranged by size: 2301 1028 910 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2496 3522 3607 4678 2496 Size: 1026 85 1071 3279 Fragments arranged by size: 3279 1071 1026 85 BsmI GAATG_Cn' Cuts at: 2466 2483 2972 3065 2466 Size: 17 489 93 4862 Fragments arranged by size: 4862 489 93 17 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2014 2496 2780 3522 3607 4678 1391 Size: 399 224 482 284 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 482 399 284 224 85 BspEI T'CCGG_A Cuts at: 5124 5124 Size: 5461 BspHI T'CATG_A Cuts at: 3350 5080 3350 Size: 1730 3731 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2816 844 Size: 1972 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3348 1394 Size: 429 1525 3507 Fragments arranged by size: 3507 1525 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3956 4130 65 Size: 1183 2708 174 1396 Fragments arranged by size: 2708 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2026 2032 4105 4292 1700 Size: 110 216 6 2073 187 2869 Fragments arranged by size: 2869 2073 216 187 110 6 BsrGI T'GTAC_A Cuts at: 96 2746 96 Size: 2650 2811 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5461 BssSI C'ACGA_G Cuts at: 3521 4533 3521 Size: 1012 4449 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5461 BstBI TT'CG_AA Cuts at: 4265 4265 Size: 5461 BstEII G'GTnAC_C Cuts at: 1184 2155 1184 Size: 971 4490 BstYI r'GATC_y Cuts at: 1972 2791 3158 3543 3560 5012 5098 5110 5456 Size: 819 367 385 17 1452 86 12 346 Cuts at: 5456 1972 Size: 1977 Fragments arranged by size: 1977 1452 819 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4909 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4722 Fragments arranged by size: 4722 683 56 BtsI GCAGTG_nn' Cuts at: 2977 3771 3791 2977 Size: 794 20 4647 Fragments arranged by size: 4647 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5461 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5426 DraI TTT'AAA Cuts at: 2765 2765 Size: 5461 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4468 809 Size: 507 3152 1802 Fragments arranged by size: 3152 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2908 3797 5426 Size: 54 1425 50 275 18 1078 889 1629 Cuts at: 5426 8 Size: 43 Fragments arranged by size: 1629 1425 1078 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2908 1537 Size: 275 1096 4090 Fragments arranged by size: 4090 1096 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2253 3391 1353 Size: 900 1138 3423 Fragments arranged by size: 3423 1138 900 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4048 4422 4568 387 Size: 1064 138 2459 374 146 1280 Fragments arranged by size: 2459 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2163 2349 3524 4121 4908 5176 1239 Size: 393 531 186 1175 597 787 268 1524 Fragments arranged by size: 1524 1175 787 597 531 393 268 186 EcoRI G'AATT_C Cuts at: 2770 3148 2770 Size: 378 5083 FspI TGC'GCA Cuts at: 3967 3967 Size: 5461 HaeII r_GCGC'y Cuts at: 1678 2033 4608 1678 Size: 355 2575 2531 Fragments arranged by size: 2575 2531 355 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2073 2087 2105 2119 2670 Size: 32 135 32 501 14 18 14 551 Cuts at: 2670 2702 4102 4134 4176 4208 1373 Size: 32 1400 32 42 32 2626 Fragments arranged by size: 2626 1400 551 501 135 42 32 32 32 32 32 18 14 14 HincII GTy'rAC Cuts at: 669 1657 1938 2805 3051 669 Size: 988 281 867 246 3079 Fragments arranged by size: 3079 988 867 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5461 HpaI GTT'AAC Cuts at: 3051 3051 Size: 5461 KasI G'GCGC_C Cuts at: 2029 2029 Size: 5461 KpnI G_GTAC'C Cuts at: 2786 2786 Size: 5461 MfeI C'AATT_G Cuts at: 3060 3060 Size: 5461 MluI A'CGCG_T Cuts at: 5337 5337 Size: 5461 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4575 4759 1305 Size: 3270 184 2007 Fragments arranged by size: 3270 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3639 Fragments arranged by size: 3639 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2515 3419 3778 3937 518 Size: 648 1349 904 359 159 2042 Fragments arranged by size: 2042 1349 904 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2017 2648 2877 3554 4702 4947 1606 Size: 411 631 229 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 631 411 245 229 NaeI GCC'GGC Cuts at: 2028 2028 Size: 5461 NarI GG'CG_CC Cuts at: 2030 2030 Size: 5461 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4909 NdeI CA'TA_TG Cuts at: 387 387 Size: 5461 NgoMIV G'CCGG_C Cuts at: 2026 2026 Size: 5461 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5461 NotI GC'GGCC_GC Cuts at: 2908 2908 Size: 5461 NspI r_CATG'y Cuts at: 1132 1229 2514 2753 2819 4364 1132 Size: 97 1285 239 66 1545 2229 Fragments arranged by size: 2229 1545 1285 239 97 66 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5461 PciI A'CATG_T Cuts at: 4360 4360 Size: 5461 PflMI CCAn_nnn'nTGG Cuts at: 1108 2126 1108 Size: 1018 4443 PmeI GTTT'AAAC Cuts at: 2765 2765 Size: 5461 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3530 3562 5043 5075 3530 Size: 32 1481 32 3916 Fragments arranged by size: 3916 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5461 PsiI TTA'TAA Cuts at: 3031 3031 Size: 5461 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2265 2297 2265 Size: 32 5429 PstI C_TGCA'G Cuts at: 830 2813 830 Size: 1983 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5461 PvuII CAG'CTG Cuts at: 2648 2648 Size: 5461 SalI G'TCGA_C Cuts at: 1936 2803 1936 Size: 867 4594 SbfI CC_TGCA'GG Cuts at: 2813 2813 Size: 5461 ScaI AGT'ACT Cuts at: 3709 5421 3709 Size: 1712 3749 SfcI C'TryA_G Cuts at: 826 1045 2754 2809 3944 4625 4816 826 Size: 219 1709 55 1135 681 191 1471 Fragments arranged by size: 1709 1471 1135 681 219 191 55 SfoI GGC'GCC Cuts at: 2031 2031 Size: 5461 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5461 SgrAI Cr'CCGG_yG Cuts at: 1700 2026 2032 1700 Size: 326 6 5129 Fragments arranged by size: 5129 326 6 SmaI CCC'GGG Cuts at: 1872 2788 1872 Size: 916 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2161 3565 4466 4728 820 Size: 197 926 218 1404 901 262 1553 Fragments arranged by size: 1553 1404 926 901 262 218 197 SnaBI TAC'GTA Cuts at: 493 3252 493 Size: 2759 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5461 SphI G_CATG'C Cuts at: 1132 2819 1132 Size: 1687 3774 SspI AAT'ATT Cuts at: 5 52 2531 3385 5 Size: 47 2479 854 2081 Fragments arranged by size: 2479 2081 854 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5461 StyI C'CwwG_G Cuts at: 513 1065 2900 513 Size: 552 1835 3074 Fragments arranged by size: 3074 1835 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3488 3505 3658 3843 4288 325 Size: 1255 1908 17 153 185 445 1498 Fragments arranged by size: 1908 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2746 3707 5419 Size: 274 80 33 51 1443 769 961 1712 Cuts at: 5419 96 Size: 138 Fragments arranged by size: 1712 1443 961 769 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5421 5453 5421 Size: 32 5429 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5461 XbaI T'CTAG_A Cuts at: 2797 2797 Size: 5461 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2075 2141 2075 Size: 66 5395 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5461 XmaI C'CCGG_G Cuts at: 1870 2786 1870 Size: 916 4545 XmnI GAAnn'nnTTC Cuts at: 3590 3590 Size: 5461 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3269 276 Size: 53 83 186 936 1735 2468 Fragments arranged by size: 2468 1735 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI