(Circular) (Six-base) MAPSORT of: pFN21AB6694.seq Check: 9856 from: 1 to: 6091 pFN21AB6694 6091 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3901 278 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2763 3434 1937 Size: 826 671 4594 Fragments arranged by size: 4594 826 671 Acc65I G'GTAC_C Cuts at: 3412 3412 Size: 6091 AclI AA'CG_TT Cuts at: 1563 2856 2976 4218 4591 1563 Size: 1293 120 1242 373 3063 Fragments arranged by size: 3063 1293 1242 373 120 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3149 4154 5538 5806 1632 Size: 1517 1005 1384 268 1917 Fragments arranged by size: 1917 1517 1384 1005 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5894 AflIII A'CryG_T Cuts at: 1203 2047 4990 5967 1203 Size: 844 2943 977 1327 Fragments arranged by size: 2943 1327 977 844 AgeI A'CCGG_T Cuts at: 4922 4922 Size: 6091 AhdI GACnn_n'nnGTC Cuts at: 4820 4820 Size: 6091 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6091 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6091 AlwNI CAG_nnn'CTG Cuts at: 3834 5406 3834 Size: 1572 4519 ApaLI G'TGCA_C Cuts at: 4148 5304 4148 Size: 1156 4935 ApoI r'AATT_y Cuts at: 1493 2126 2240 2369 3179 3400 3626 3778 5887 Size: 633 114 129 810 221 226 152 2109 Cuts at: 5887 1493 Size: 1697 Fragments arranged by size: 2109 1697 810 633 226 221 152 129 114 AseI AT'TA_AT Cuts at: 160 1032 4645 160 Size: 872 3613 1606 Fragments arranged by size: 3613 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 AvaI C'yCGr_G Cuts at: 1870 1943 2403 3416 1870 Size: 73 460 1013 4545 Fragments arranged by size: 4545 1013 460 73 BamHI G'GATC_C Cuts at: 3421 3788 3421 Size: 367 5724 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3412 4867 618 Size: 325 217 627 1625 1455 1842 Fragments arranged by size: 1842 1625 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2162 3410 1391 Size: 771 1248 4072 Fragments arranged by size: 4072 1248 771 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5120 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4282 4316 1903 Size: 34 2345 34 3678 Fragments arranged by size: 3678 2345 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3983 5193 3983 Size: 1210 4881 BclI T'GATC_A Cuts at: 1548 1548 Size: 6091 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2420 4702 136 Size: 107 122 71 631 605 748 2282 1525 Fragments arranged by size: 2282 1525 748 631 605 122 107 71 BglII A'GATC_T Cuts at: 6086 6086 Size: 6091 BlpI GC'TnA_GC Cuts at: 2199 3123 3508 2199 Size: 924 385 4782 Fragments arranged by size: 4782 924 385 Bme1580I G_kGCm'C Cuts at: 1790 4152 5308 1790 Size: 2362 1156 2573 Fragments arranged by size: 2573 2362 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6091 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2441 3822 4780 Size: 416 53 83 399 24 1024 1381 958 Cuts at: 4780 442 Size: 1753 Fragments arranged by size: 1753 1381 1024 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6091 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2925 3265 2925 Size: 340 5751 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2630 2738 2832 3363 4751 1239 Size: 1391 108 94 531 1388 2579 Fragments arranged by size: 2579 1391 1388 531 108 94 Bpu10I CC'TnA_GC Cuts at: 3030 3030 Size: 6091 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2711 4216 5081 5379 2711 Size: 1505 865 298 3423 Fragments arranged by size: 3423 1505 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2240 4754 882 Size: 617 741 2514 2219 Fragments arranged by size: 2514 2219 741 617 BsaAI yAC'GTr Cuts at: 493 1204 2050 3882 493 Size: 711 846 1832 2702 Fragments arranged by size: 2702 1832 846 711 BsaBI GATnn'nnATC Cuts at: 6085 6085 Size: 6091 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3898 4280 4915 275 Size: 53 83 186 936 2365 382 635 1451 Fragments arranged by size: 2365 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4524 4922 5196 5343 5754 4524 Size: 398 274 147 411 4861 Fragments arranged by size: 4861 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2830 2860 1178 Size: 30 165 30 1427 30 4409 Fragments arranged by size: 4409 1427 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6091 BseYI C'CCAG_C Cuts at: 5294 5790 5294 Size: 496 5595 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5518 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3541 4302 5330 1540 Size: 275 186 1540 761 1028 2301 Fragments arranged by size: 2301 1540 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4152 4237 5308 4152 Size: 85 1071 4935 Fragments arranged by size: 4935 1071 85 BsmI GAATG_Cn' Cuts at: 3602 3695 3602 Size: 93 5998 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2162 3410 4152 4237 5308 1391 Size: 399 372 1248 742 85 1071 2174 Fragments arranged by size: 2174 1248 1071 742 399 372 85 BspEI T'CCGG_A Cuts at: 5754 5754 Size: 6091 BspHI T'CATG_A Cuts at: 3980 5710 3980 Size: 1730 4361 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3446 844 Size: 2602 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3978 1394 Size: 429 2155 3507 Fragments arranged by size: 3507 2155 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2674 3105 4586 4760 65 Size: 1183 1426 431 1481 174 1396 Fragments arranged by size: 1481 1426 1396 1183 431 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3068 4735 4922 1700 Size: 110 1258 1667 187 2869 Fragments arranged by size: 2869 1667 1258 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6091 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6091 BssSI C'ACGA_G Cuts at: 4151 5163 4151 Size: 1012 5079 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6091 BstBI TT'CG_AA Cuts at: 4895 4895 Size: 6091 BstEII G'GTnAC_C Cuts at: 1184 2836 3355 1184 Size: 1652 519 3920 Fragments arranged by size: 3920 1652 519 BstYI r'GATC_y Cuts at: 1972 3421 3788 4173 4190 5642 5728 5740 6086 Size: 1449 367 385 17 1452 86 12 346 Cuts at: 6086 1972 Size: 1977 Fragments arranged by size: 1977 1452 1449 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4600 Fragments arranged by size: 4600 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5352 Fragments arranged by size: 5352 683 56 BtsI GCAGTG_nn' Cuts at: 3024 3330 3607 4401 4421 3024 Size: 306 277 794 20 4694 Fragments arranged by size: 4694 794 306 277 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6091 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2656 2671 2691 2706 2755 2790 529 Size: 35 2092 15 20 15 49 35 3830 Fragments arranged by size: 3830 2092 49 35 35 20 15 15 DraI TTT'AAA Cuts at: 3395 3395 Size: 6091 DraIII CAC_nnn'GTG Cuts at: 2435 2435 Size: 6091 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2339 5098 809 Size: 507 1023 2759 1802 Fragments arranged by size: 2759 1802 1023 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2077 2797 3538 Size: 54 1425 50 275 18 247 720 741 Cuts at: 3538 4427 6056 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 741 720 275 247 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3538 1537 Size: 275 1726 4090 Fragments arranged by size: 4090 1726 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2492 2916 4021 1353 Size: 1139 424 1105 3423 Fragments arranged by size: 3423 1139 1105 424 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4678 5052 5198 387 Size: 1064 138 3089 374 146 1280 Fragments arranged by size: 3089 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2630 2738 2832 3149 3363 4154 4751 Size: 393 998 108 94 317 214 791 597 Cuts at: 4751 5538 5806 1239 Size: 787 268 1524 Fragments arranged by size: 1524 998 791 787 597 393 317 268 214 108 94 EcoRI G'AATT_C Cuts at: 3400 3778 3400 Size: 378 5713 EcoRV GAT'ATC Cuts at: 2227 2227 Size: 6091 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2179 2179 Size: 6091 FspI TGC'GCA Cuts at: 4597 4597 Size: 6091 HaeII r_GCGC'y Cuts at: 1678 3069 5238 1678 Size: 1391 2169 2531 Fragments arranged by size: 2531 2169 1391 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2283 2315 2634 2666 4732 Size: 32 135 32 711 32 319 32 2066 Cuts at: 4732 4764 4806 4838 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2066 711 319 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2461 2854 3435 3681 669 Size: 988 281 523 393 581 246 3079 Fragments arranged by size: 3079 988 581 523 393 281 246 HindIII A'AGCT_T Cuts at: 748 2895 748 Size: 2147 3944 HpaI GTT'AAC Cuts at: 2461 3681 2461 Size: 1220 4871 KpnI G_GTAC'C Cuts at: 3416 3416 Size: 6091 MfeI C'AATT_G Cuts at: 3690 3690 Size: 6091 MluI A'CGCG_T Cuts at: 5967 5967 Size: 6091 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2570 2750 5205 5389 1305 Size: 1265 180 2455 184 2007 Fragments arranged by size: 2455 2007 1265 184 180 MscI TGG'CCA Cuts at: 10 64 1489 1832 2079 10 Size: 54 1425 343 247 4022 Fragments arranged by size: 4022 1425 343 247 54 MslI CAynn'nnrTG Cuts at: 518 1166 4049 4408 4567 518 Size: 648 2883 359 159 2042 Fragments arranged by size: 2883 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3017 3507 4184 5332 5577 1606 Size: 1411 490 677 1148 245 2120 Fragments arranged by size: 2120 1411 1148 677 490 245 NaeI GCC'GGC Cuts at: 3070 3070 Size: 6091 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4600 Fragments arranged by size: 4600 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6091 NgoMIV G'CCGG_C Cuts at: 3068 3068 Size: 6091 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6091 NotI GC'GGCC_GC Cuts at: 3538 3538 Size: 6091 NspI r_CATG'y Cuts at: 1132 1229 2414 2608 2629 3114 3449 4994 1132 Size: 97 1185 194 21 485 335 1545 2229 Fragments arranged by size: 2229 1545 1185 485 335 194 97 21 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6091 PciI A'CATG_T Cuts at: 4990 4990 Size: 6091 PflMI CCAn_nnn'nTGG Cuts at: 1108 2585 1108 Size: 1477 4614 PfoI T'CCnGG_A Cuts at: 2754 2754 Size: 6091 PmeI GTTT'AAAC Cuts at: 3395 3395 Size: 6091 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4160 4192 5673 5705 4160 Size: 32 1481 32 4546 Fragments arranged by size: 4546 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6091 PsiI TTA'TAA Cuts at: 3661 3661 Size: 6091 PstI C_TGCA'G Cuts at: 830 3443 830 Size: 2613 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6091 PvuII CAG'CTG Cuts at: 3017 3017 Size: 6091 SalI G'TCGA_C Cuts at: 1936 3433 1936 Size: 1497 4594 SapI GCTCTTCn'nnn_ Cuts at: 2916 2916 Size: 6091 SbfI CC_TGCA'GG Cuts at: 3443 3443 Size: 6091 ScaI AGT'ACT Cuts at: 2258 4339 6051 2258 Size: 2081 1712 2298 Fragments arranged by size: 2298 2081 1712 SfcI C'TryA_G Cuts at: 826 1045 2488 3439 4574 5255 5446 826 Size: 219 1443 951 1135 681 191 1471 Fragments arranged by size: 1471 1443 1135 951 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6091 SmaI CCC'GGG Cuts at: 1872 3418 1872 Size: 1546 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2690 4195 5096 5358 820 Size: 197 926 747 1505 901 262 1553 Fragments arranged by size: 1553 1505 926 901 747 262 197 SnaBI TAC'GTA Cuts at: 493 3882 493 Size: 3389 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6091 SphI G_CATG'C Cuts at: 1132 2414 3114 3449 1132 Size: 1282 700 335 3774 Fragments arranged by size: 3774 1282 700 335 SspI AAT'ATT Cuts at: 5 52 4015 5 Size: 47 3963 2081 Fragments arranged by size: 3963 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6091 StyI C'CwwG_G Cuts at: 513 1065 2004 2630 3530 513 Size: 552 939 626 900 3074 Fragments arranged by size: 3074 939 900 626 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2423 3299 4118 4135 4288 4473 4918 Size: 1255 843 876 819 17 153 185 445 Cuts at: 4918 325 Size: 1498 Fragments arranged by size: 1498 1255 876 843 819 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2256 4337 6049 Size: 274 80 33 51 1443 279 2081 1712 Cuts at: 6049 96 Size: 138 Fragments arranged by size: 2081 1712 1443 279 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2830 2862 6051 6083 2830 Size: 32 3189 32 2838 Fragments arranged by size: 3189 2838 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6091 XbaI T'CTAG_A Cuts at: 3427 3427 Size: 6091 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2586 3211 2586 Size: 625 5466 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6091 XmaI C'CCGG_G Cuts at: 1870 3416 1870 Size: 1546 4545 XmnI GAAnn'nnTTC Cuts at: 2910 4220 2910 Size: 1310 4781 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3899 276 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI