################################## # NO CUT ENZYMES ( 172 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BbvI BcgI BciVI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I Fnu4HI FseI FspI HgaI Hin1I HindIII HpaI ItaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MflI MluI MroI MseI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I TseI Tsp509I Tth111I TthHB8I Van91I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AlwI 99 GATCC GGATC(4/5) AlwNI 330 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 71 GGGCCC GGGCC^C AvaI 127 CCCGGG C^[CT]CG[GA]G AvaII 240 GGACC G^G[AT]CC BalI 416 TGGCCA TGG^CCA BbsI 148 GTCTTC GAAGAC(2/6) BclI 272 TGATCA T^GATCA BpuAI 148 GTCTTC GAAGAC(2/6) BsaAI 94 TACGTG [CT]AC^GT[GA] BsaI 32 GAGACC GGTCTC(1/5) BsgI 170 GTGCAG GTGCAG(16/14) BsmAI 32 GAGAC GTCTC(1/5) BsmFI 372 GTCCC GGGAC(10/14) BsoBI 127 CCCGGG C^[CT]CG[GA]G BstF5I 448 GGATG GGATG(2/0) Cac8I 133 GCTGGC GC[ACGT]^[ACGT]GC DraII 71 GGGCCCT [GA]G^G[ACGT]CC[CT] EaeI 416 TGGCCA [CT]^GGCC[GA] EcoO109I 71 GGGCCCT [GA]G^G[ACGT]CC[CT] FauI 374 CCCGC CCCGC(4/6) FbaI 272 TGATCA T^GATCA FokI 448 GGATG GGATG(9/13) HapII 128 CCGG C^CGG HincII 226 GTCAAC GT[CT]^[GA]AC HindII 226 GTCAAC GT[CT]^[GA]AC HinfI 431 GAATC G^A[ACGT]TC HpaII 128 CCGG C^CGG MaeII 95 ACGT A^CGT MaeIII 349 GTCAC ^GT[ACGT]AC MluNI 416 TGGCCA TGG^CCA MscI 416 TGGCCA TGG^CCA MspI 128 CCGG C^CGG MwoI 368 GCAAGTCCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIII 244 CATG CATG^ PvuII 355 CAGCTG CAG^CTG SfaNI 449 GATGC GCATC(5/9) SmaI 127 CCCGGG CCC^GGG TfiI 431 GAATC G^A[AT]TC Tsp45I 349 GTCAC ^GT[GC]AC XcmI 354 CCAGCTGAGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 127 CCCGGG C^CCGGG XmaI 127 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AciI 51 CCGC CCGC(-3/-1) 375 CCGC AluI 235 AGCT AG^CT 356 AGCT AspHI 64 GAGCAC G[AT]GC[AT]^C 340 GAGCAC BanII 19 GAGCCC G[GA]GC[CT]^C 71 GGGCCC BcnI 127 CCCGG CC^[GC]GG 128 CCGGG BpmI 141 CTCCAG CTGGAG(16/14) 29 CTGGAG BseRI 110 CTCCTC GAGGAG(10/8) 138 CTCCTC BsiHKAI 64 GAGCAC G[AT]GC[AT]^C 340 GAGCAC BsiYI 22 CCCAAACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 127 CCCGGGGCTGG BslI 22 CCCAAACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 127 CCCGGGGCTGG BsrI 68 ACTGG ACTGG(1/-1) 143 CCAGT CfoI 189 GCGC GCG^C 424 GCGC DpnII 99 GATC ^GATC 273 GATC EcoT14I 54 CCTTGG C^C[AT][AT]GG 398 CCAAGG HaeII 188 AGCGCC [GA]GCGC^[CT] 423 GGCGCT HhaI 189 GCGC GCG^C 424 GCGC HinP1I 189 GCGC G^CGC 424 GCGC MboI 99 GATC ^GATC 273 GATC MspA1I 355 CAGCTG C[AC]G^C[GT]G 375 CCGCTG NciI 127 CCCGG CC^[GC]GG 128 CCGGG NdeII 99 GATC ^GATC 273 GATC Sau3AI 99 GATC ^GATC 273 GATC StyI 54 CCTTGG C^C[AT][AT]GG 398 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 20 enzymes) # ######################################### AfaI BmyI BsaJI Bsp1286I BssKI BstNI Cfr13I DdeI DsaI EcoRII HaeIII HphI MboII MnlI MvaI NlaIV RsaI Sau96I ScrFI TspRI