################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BciVI BcnI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstXI Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I FseI FspI HgaI Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI KspI MaeI MamI MfeI MluI MroI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StyI SwaI TaqI TfiI Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccI 204 GTATAC GT^[AC][GT]AC AccII 389 CGCG CG^CG AcsI 912 GAATTT [GA]^AATT[CT] AlwNI 356 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 668 GGGCCC GGGCC^C ApoI 912 GAATTT [GA]^AATT[CT] AvaII 852 GGACC G^G[AT]CC BanI 811 GGCACC G^G[CT][GA]CC BbsI 877 GAAGAC GAAGAC(2/6) BglI 119 GCCTGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 877 GAAGAC GAAGAC(2/6) BsaWI 957 TCCGGT [AT]^CCGG[AT] BseRI 662 GAGGAG GAGGAG(10/8) BsmAI 738 GAGAC GTCTC(1/5) BsmI 1133 GAATGC GAATGC(1/-1) BspMI 379 ACCTGC ACCTGC(4/8) BsrDI 460 GCAATG GCAATG(2/0) Bst1107I 204 GTATAC GTA^TAC BstUI 389 CGCG CG^CG BstZ17I 204 GTATAC GTA^TAC EcoT22I 561 ATGCAT ATGCA^T HaeII 1027 GGCGCT [GA]GCGC^[CT] MaeII 604 ACGT A^CGT MseI 764 TTAA T^TAA MslI 564 CATGGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 389 CGCG CG^CG NsiI 561 ATGCAT ATGCA^T NspI 978 GCATGC [GA]CATG^[CT] PflMI 520 CCAACTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 466 CTGCAG CTGCA^G PvuII 97 CAGCTG CAG^CTG ScaI 51 AGTACT AGT^ACT SfcI 466 CTGCAG C^T[GA][CT]AG SphI 978 GCATGC GCATG^C StuI 126 AGGCCT AGG^CCT Tru9I 764 TTAA T^TAA Van91I 520 CCAACTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 629 CCAAAGGGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AfaI 52 GTAC GT^AC 871 GTAC BbvCI 361 GCTGAGG CCTCAGC(-5/-2) 865 GCTGAGG BcgI 373 CGAAGAACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 419 CGAAGCCAGTGC BclI 491 TGATCA T^GATCA 926 TGATCA BsaJI 354 CCCAGG C^C[ACGT][ACGT]GG 1018 CCAGGG BsgI 134 GTGCAG GTGCAG(16/14) 427 GTGCAG BstYI 29 GGATCT [GA]^GATC[CT] 1040 GGATCT CfoI 390 GCGC GCG^C 1028 GCGC DraII 667 GGGGCCC [GA]G^G[ACGT]CC[CT] 668 GGGCCCC EcoO109I 667 GGGGCCC [GA]G^G[ACGT]CC[CT] 668 GGGCCCC FbaI 491 TGATCA T^GATCA 926 TGATCA HapII 332 CCGG C^CGG 958 CCGG HhaI 390 GCGC GCG^C 1028 GCGC HinP1I 390 GCGC G^CGC 1028 GCGC HpaII 332 CCGG C^CGG 958 CCGG MflI 29 GGATCT [GA]^GATC[CT] 1040 GGATCT MspI 332 CCGG C^CGG 958 CCGG RsaI 52 GTAC GT^AC 871 GTAC SacI 87 GAGCTC GAGCT^C 1124 GAGCTC SapI 84 GAAGAGC GCTCTTC(1/4) 993 GAAGAGC SfaNI 439 GATGC GCATC(5/9) 688 GCATC TspRI 425 CAGTG CA[GC]TG[ACGT][ACGT]^ 816 CAGTG XhoII 29 GGATCT [GA]^GATC[CT] 1040 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AciI AluI AlwI AspHI BalI BanII BbvI BmyI BpmI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EaeI EarI EcoRII FauI Fnu4HI FokI HaeIII HphI ItaI Ksp632I MaeIII MboI MboII MluNI MnlI MscI MspA1I MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TseI Tsp45I Tsp509I