################################## # NO CUT ENZYMES ( 153 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 26 enzymes) # ################################### AflIII 125 ACATGT A^C[GA][CT]GT Alw44I 29 GTGCAC G^TGCAC ApaLI 29 GTGCAC G^TGCAC BanI 399 GGCACC G^G[CT][GA]CC BbsI 589 GAAGAC GAAGAC(2/6) BglI 559 GCCTCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 461 CTCCAG CTGGAG(16/14) BpuAI 589 GAAGAC GAAGAC(2/6) BsmFI 775 GGGAC GGGAC(10/14) BsmI 794 GCATTC GAATGC(1/-1) BspLU11I 125 ACATGT A^CATGT BspMI 593 ACCTGC ACCTGC(4/8) BstF5I 661 CATCC GGATG(2/0) BstXI 860 CCAATGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraIII 401 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 1072 CCTGGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 160 GAATTC G^AATTC FauI 599 CCCGC CCCGC(4/6) FokI 661 CATCC GGATG(9/13) MslI 580 CATAACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 224 CCATGG C^CATGG PflMI 219 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 215 GAGTC GAGTC(4/5) SexAI 402 ACCTGGT A^CC[AT]GGT SfaNI 100 GCATC GCATC(5/9) Van91I 219 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AciI 435 CCGC CCGC(-3/-1) 600 CCGC AcsI 160 GAATTC [GA]^AATT[CT] 523 GAATTT AfaI 712 GTAC GT^AC 763 GTAC ApoI 160 GAATTC [GA]^AATT[CT] 523 GAATTT AseI 769 ATTAAT AT^TAAT 849 ATTAAT AsnI 769 ATTAAT AT^TAAT 849 ATTAAT BanII 446 GAGCTC G[GA]GC[CT]^C 880 GAGCTC BfaI 541 CTAG C^TAG 877 CTAG BsgI 842 CTGCAC GTGCAG(16/14) 1178 CTGCAC BstYI 818 AGATCC [GA]^GATC[CT] 1202 AGATCC DsaI 224 CCATGG C^C[GA][CT]GG 758 CCACGG HincII 9 GTTGAC GT[CT]^[GA]AC 1247 GTCAAC HindII 9 GTTGAC GT[CT]^[GA]AC 1247 GTCAAC MaeI 541 CTAG C^TAG 877 CTAG MflI 818 AGATCC [GA]^GATC[CT] 1202 AGATCC MspA1I 1027 CAGCTG C[AC]G^C[GT]G 1175 CAGCTG NspI 125 ACATGT [GA]CATG^[CT] 1188 ACATGC PshBI 769 ATTAAT AT^TAAT 849 ATTAAT PvuII 1027 CAGCTG CAG^CTG 1175 CAGCTG RsaI 712 GTAC GT^AC 763 GTAC SacI 446 GAGCTC GAGCT^C 880 GAGCTC SfcI 62 CTACAG C^T[GA][CT]AG 147 CTATAG SmlI 137 CTCAAG C^T[CT][GA]AG 905 CTCAAG SspI 626 AATATT AAT^ATT 923 AATATT StuI 66 AGGCCT AGG^CCT 295 AGGCCT TaqI 426 TCGA T^CGA 441 TCGA TfiI 72 GATTC G^A[AT]TC 180 GAATC TthHB8I 426 TCGA T^CGA 441 TCGA XcmI 371 CCAGCCTGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 857 CCACCAATGCCTTGG XhoII 818 AGATCC [GA]^GATC[CT] 1202 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AluI AlwI AspHI AvaII BbvI BmyI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII EcoT14I Fnu4HI HaeIII HinfI HphI ItaI Ksp632I MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI StyI Tru9I TseI Tsp45I Tsp509I TspRI