(Linear) (Six-base) MAPSORT of: KIEE6316.seq Check: 1709 from: 1 to: 1254 KIEE6316.orf With 182 enzymes: SgfI * March 16, 2015 11:29 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1051 1254 Size: 1051 203 AflIII A'CryG_T Cuts at: 0 125 1254 Size: 125 1129 ApaLI G'TGCA_C Cuts at: 0 29 1254 Size: 29 1225 ApoI r'AATT_y Cuts at: 0 160 523 1254 Size: 160 363 731 Fragments arranged by size: 731 363 160 AseI AT'TA_AT Cuts at: 0 770 850 1254 Size: 770 80 404 Fragments arranged by size: 770 404 80 BanI G'GyrC_C Cuts at: 0 399 1254 Size: 399 855 BanII G_rGCy'C Cuts at: 0 450 884 1254 Size: 450 434 370 Fragments arranged by size: 450 434 370 BbsI GAAGACnn'nnnn_ Cuts at: 0 596 1254 Size: 596 658 BglI GCCn_nnn'nGGC Cuts at: 0 565 1254 Size: 565 689 Bme1580I G_kGCm'C Cuts at: 0 33 1254 Size: 33 1221 BmrI ACTGGGnnnn_n' Cuts at: 0 1158 1254 Size: 1158 96 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 466 490 1254 Size: 466 24 764 Fragments arranged by size: 764 466 24 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 446 1254 Size: 446 808 Bpu10I CC'TnA_GC Cuts at: 0 678 1254 Size: 678 576 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 122 890 1254 Size: 122 768 364 Fragments arranged by size: 768 364 122 BsaI GGTCTCn'nnnn_ Cuts at: 0 235 245 478 1254 Size: 235 10 233 776 Fragments arranged by size: 776 235 233 10 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 376 406 1145 1175 1254 Size: 376 30 739 30 79 Fragments arranged by size: 739 376 79 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 458 649 1097 1169 1254 Size: 458 191 448 72 85 Fragments arranged by size: 458 448 191 85 72 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 827 1163 1254 Size: 827 336 91 Fragments arranged by size: 827 336 91 BsiHKAI G_wGCw'C Cuts at: 0 33 363 450 884 1254 Size: 33 330 87 434 370 Fragments arranged by size: 434 370 330 87 33 BsmI GAATG_Cn' Cuts at: 0 794 1254 Size: 794 460 Bsp1286I G_dGCh'C Cuts at: 0 33 363 450 884 1254 Size: 33 330 87 434 370 Fragments arranged by size: 434 370 330 87 33 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 602 1254 Size: 602 652 BstXI CCAn_nnnn'nTGG Cuts at: 0 867 1254 Size: 867 387 BstYI r'GATC_y Cuts at: 0 818 1202 1254 Size: 818 384 52 Fragments arranged by size: 818 384 52 BtgI C'CryG_G Cuts at: 0 224 758 1254 Size: 224 534 496 Fragments arranged by size: 534 496 224 DraIII CAC_nnn'GTG Cuts at: 0 406 1254 Size: 406 848 EarI CTCTTCn'nnn_ Cuts at: 0 238 481 935 967 1027 1254 Size: 238 243 454 32 60 227 Fragments arranged by size: 454 243 238 227 60 32 EcoICRI GAG'CTC Cuts at: 0 448 882 1254 Size: 448 434 372 Fragments arranged by size: 448 434 372 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 446 1051 1254 Size: 446 605 203 Fragments arranged by size: 605 446 203 EcoNI CCTnn'n_nnAGG Cuts at: 0 1076 1254 Size: 1076 178 EcoRI G'AATT_C Cuts at: 0 160 1254 Size: 160 1094 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 334 454 1254 Size: 334 120 800 Fragments arranged by size: 800 334 120 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1080 1112 1148 1180 1254 Size: 1080 32 36 32 74 Fragments arranged by size: 1080 74 36 32 32 HincII GTy'rAC Cuts at: 0 11 1249 1254 Size: 11 1238 5 Fragments arranged by size: 1238 11 5 MslI CAynn'nnrTG Cuts at: 0 584 1254 Size: 584 670 MspA1I CmG'CkG Cuts at: 0 1029 1177 1254 Size: 1029 148 77 Fragments arranged by size: 1029 148 77 NcoI C'CATG_G Cuts at: 0 224 1254 Size: 224 1030 NspI r_CATG'y Cuts at: 0 129 1192 1254 Size: 129 1063 62 Fragments arranged by size: 1063 129 62 PasI CC'CwG_GG Cuts at: 0 481 948 1072 1254 Size: 481 467 124 182 Fragments arranged by size: 481 467 182 124 PciI A'CATG_T Cuts at: 0 125 1254 Size: 125 1129 PflMI CCAn_nnn'nTGG Cuts at: 0 225 1254 Size: 225 1029 PfoI T'CCnGG_A Cuts at: 0 462 1254 Size: 462 792 PvuII CAG'CTG Cuts at: 0 1029 1177 1254 Size: 1029 148 77 Fragments arranged by size: 1029 148 77 SacI G_AGCT'C Cuts at: 0 450 884 1254 Size: 450 434 370 Fragments arranged by size: 450 434 370 SexAI A'CCwGG_T Cuts at: 0 402 1254 Size: 402 852 SfcI C'TryA_G Cuts at: 0 62 147 1254 Size: 62 85 1107 Fragments arranged by size: 1107 85 62 SmlI C'TyrA_G Cuts at: 0 137 905 1254 Size: 137 768 349 Fragments arranged by size: 768 349 137 SspI AAT'ATT Cuts at: 0 628 925 1254 Size: 628 297 329 Fragments arranged by size: 628 329 297 StuI AGG'CCT Cuts at: 0 68 297 1254 Size: 68 229 957 Fragments arranged by size: 957 229 68 StyI C'CwwG_G Cuts at: 0 224 563 569 866 1254 Size: 224 339 6 297 388 Fragments arranged by size: 388 339 297 224 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 499 1254 Size: 499 755 TatI w'GTAC_w Cuts at: 0 711 1254 Size: 711 543 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1145 1177 1254 Size: 1145 32 77 Fragments arranged by size: 1145 77 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 378 864 1254 Size: 378 486 390 Fragments arranged by size: 486 390 378 Enzymes that do cut: AcuI AflIII ApaLI ApoI AseI BanI BanII BbsI BglI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspMI BstXI BstYI BtgI DraIII EarI EcoICRI Eco57MI EcoNI EcoRI FalI Hin4I HincII MslI MspA1I NcoI NspI PasI PciI PflMI PfoI PvuII SacI SexAI SfcI SmlI SspI StuI StyI TaqII TatI TstI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaI AscI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EciI EcoO109I EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI