(Circular) (Six-base) MAPSORT of: pFN21AB7986.seq Check: 3690 from: 1 to: 5680 pFN21AB7986 5680 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3490 278 Size: 53 83 186 936 1954 2468 Fragments arranged by size: 2468 1954 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2875 3023 1937 Size: 938 148 4594 Fragments arranged by size: 4594 938 148 Acc65I G'GTAC_C Cuts at: 2769 3001 2769 Size: 232 5448 AclI AA'CG_TT Cuts at: 1563 3807 4180 1563 Size: 2244 373 3063 Fragments arranged by size: 3063 2244 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2688 2918 3743 5127 5395 1632 Size: 1056 230 825 1384 268 1917 Fragments arranged by size: 1917 1384 1056 825 268 230 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5483 AflIII A'CryG_T Cuts at: 1203 4579 5556 1203 Size: 3376 977 1327 Fragments arranged by size: 3376 1327 977 AgeI A'CCGG_T Cuts at: 2977 4511 2977 Size: 1534 4146 AhdI GACnn_n'nnGTC Cuts at: 4409 4409 Size: 5680 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5680 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5680 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2775 2807 2775 Size: 32 5648 AlwNI CAG_nnn'CTG Cuts at: 2539 3423 4995 2539 Size: 884 1572 3224 Fragments arranged by size: 3224 1572 884 ApaLI G'TGCA_C Cuts at: 2973 3737 4893 2973 Size: 764 1156 3760 Fragments arranged by size: 3760 1156 764 ApoI r'AATT_y Cuts at: 1493 2989 3215 3367 5476 1493 Size: 1496 226 152 2109 1697 Fragments arranged by size: 2109 1697 1496 226 152 AseI AT'TA_AT Cuts at: 160 1032 2052 2085 4234 160 Size: 872 1020 33 2149 1606 Fragments arranged by size: 2149 1606 1020 872 33 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5680 AvaI C'yCGr_G Cuts at: 1870 1943 2068 3005 1870 Size: 73 125 937 4545 Fragments arranged by size: 4545 937 125 73 BamHI G'GATC_C Cuts at: 3010 3377 3010 Size: 367 5313 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2769 3001 4456 618 Size: 325 217 627 982 232 1455 1842 Fragments arranged by size: 1842 1455 982 627 325 232 217 BanII G_rGCy'C Cuts at: 1391 2076 2999 1391 Size: 685 923 4072 Fragments arranged by size: 4072 923 685 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2878 928 Size: 971 979 3730 Fragments arranged by size: 3730 979 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3871 3905 1903 Size: 34 50 34 1850 34 3678 Fragments arranged by size: 3678 1850 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3572 4782 3572 Size: 1210 4470 BclI T'GATC_A Cuts at: 1548 1548 Size: 5680 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4291 136 Size: 107 122 71 631 605 2619 1525 Fragments arranged by size: 2619 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2893 5675 2893 Size: 2782 2898 BlpI GC'TnA_GC Cuts at: 2314 3097 2314 Size: 783 4897 Bme1580I G_kGCm'C Cuts at: 1790 2977 3741 4897 1790 Size: 1187 764 1156 2573 Fragments arranged by size: 2573 1187 1156 764 BmgBI CAC'GTC Cuts at: 1318 2232 1318 Size: 914 4766 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2591 2933 3411 Size: 416 53 83 399 24 1174 342 478 Cuts at: 3411 4369 442 Size: 958 1753 Fragments arranged by size: 1753 1174 958 478 416 399 342 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5680 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2529 2808 4340 1239 Size: 1290 279 1532 2579 Fragments arranged by size: 2579 1532 1290 279 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3805 4670 4968 3805 Size: 865 298 4517 Fragments arranged by size: 4517 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2023 4343 882 Size: 617 524 2320 2219 Fragments arranged by size: 2320 2219 617 524 BsaAI yAC'GTr Cuts at: 493 1204 3471 493 Size: 711 2267 2702 Fragments arranged by size: 2702 2267 711 BsaBI GATnn'nnATC Cuts at: 5674 5674 Size: 5680 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3487 3869 4504 275 Size: 53 83 186 936 1954 382 635 1451 Fragments arranged by size: 1954 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2169 2331 2943 2977 4113 4511 4785 4932 5343 Size: 162 612 34 1136 398 274 147 411 Cuts at: 5343 2169 Size: 2506 Fragments arranged by size: 2506 1136 612 411 398 274 162 147 34 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5455 Fragments arranged by size: 5455 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2108 2294 2846 1186 Size: 922 186 552 4020 Fragments arranged by size: 4020 922 552 186 BseYI C'CCAG_C Cuts at: 2139 2175 4883 5379 2139 Size: 36 2708 496 2440 Fragments arranged by size: 2708 2440 496 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5107 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3130 3891 4919 1540 Size: 275 186 1129 761 1028 2301 Fragments arranged by size: 2301 1129 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2977 3741 3826 4897 2977 Size: 764 85 1071 3760 Fragments arranged by size: 3760 1071 764 85 BsmI GAATG_Cn' Cuts at: 3191 3284 3191 Size: 93 5587 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2076 2977 2999 3741 3826 4897 1391 Size: 399 286 901 22 742 85 1071 2174 Fragments arranged by size: 2174 1071 901 742 399 286 85 22 BspEI T'CCGG_A Cuts at: 2169 5343 2169 Size: 3174 2506 BspHI T'CATG_A Cuts at: 2715 3569 5299 2715 Size: 854 1730 3096 Fragments arranged by size: 3096 1730 854 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3035 844 Size: 2191 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3567 1394 Size: 429 1744 3507 Fragments arranged by size: 3507 1744 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4175 4349 65 Size: 1183 2927 174 1396 Fragments arranged by size: 2927 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2977 4324 4511 1700 Size: 110 1167 1347 187 2869 Fragments arranged by size: 2869 1347 1167 187 110 BsrGI T'GTAC_A Cuts at: 96 2631 96 Size: 2535 3145 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5680 BssSI C'ACGA_G Cuts at: 3740 4752 3740 Size: 1012 4668 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5680 BstBI TT'CG_AA Cuts at: 4484 4484 Size: 5680 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5680 BstXI CCAn_nnnn'nTGG Cuts at: 2920 2920 Size: 5680 BstYI r'GATC_y Cuts at: 1972 2109 2221 2893 3010 3377 3762 3779 5231 Size: 137 112 672 117 367 385 17 1452 Cuts at: 5231 5317 5329 5675 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 672 385 367 346 137 117 112 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2706 2706 Size: 5680 BtgI C'CryG_G Cuts at: 513 1065 2114 513 Size: 552 1049 4079 Fragments arranged by size: 4079 1049 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4941 Fragments arranged by size: 4941 683 56 BtsI GCAGTG_nn' Cuts at: 3196 3990 4010 3196 Size: 794 20 4866 Fragments arranged by size: 4866 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5680 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2736 2771 529 Size: 35 2172 35 3438 Fragments arranged by size: 3438 2172 35 35 DraI TTT'AAA Cuts at: 2984 2984 Size: 5680 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4687 809 Size: 507 3371 1802 Fragments arranged by size: 3371 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3127 4016 5645 Size: 54 1425 50 275 18 1297 889 1629 Cuts at: 5645 8 Size: 43 Fragments arranged by size: 1629 1425 1297 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3127 1537 Size: 275 1315 4090 Fragments arranged by size: 4090 1315 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3610 1353 Size: 2257 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4267 4641 4787 387 Size: 1064 138 2678 374 146 1280 Fragments arranged by size: 2678 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2529 2688 2808 2918 3743 4340 5127 Size: 393 897 159 120 110 825 597 787 Cuts at: 5127 5395 1239 Size: 268 1524 Fragments arranged by size: 1524 897 825 787 597 393 268 159 120 110 EcoO109I rG'GnC_Cy Cuts at: 2536 2536 Size: 5680 EcoRI G'AATT_C Cuts at: 2989 3367 2989 Size: 378 5302 EcoRV GAT'ATC Cuts at: 2275 2275 Size: 5680 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2260 2260 Size: 5680 FspI TGC'GCA Cuts at: 2024 4186 2024 Size: 2162 3518 FspAI rTGC'GCAy Cuts at: 2024 2024 Size: 5680 HaeII r_GCGC'y Cuts at: 1678 4827 1678 Size: 3149 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2432 2464 4321 4353 4395 Size: 32 135 32 860 32 1857 32 42 Cuts at: 4395 4427 1373 Size: 32 2626 Fragments arranged by size: 2626 1857 860 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3024 3270 669 Size: 988 281 1086 246 3079 Fragments arranged by size: 3079 1086 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5680 HpaI GTT'AAC Cuts at: 3270 3270 Size: 5680 KpnI G_GTAC'C Cuts at: 2773 3005 2773 Size: 232 5448 MfeI C'AATT_G Cuts at: 3279 3279 Size: 5680 MluI A'CGCG_T Cuts at: 5556 5556 Size: 5680 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2082 2715 4794 4978 1305 Size: 777 633 2079 184 2007 Fragments arranged by size: 2079 2007 777 633 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3858 Fragments arranged by size: 3858 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2018 2597 3638 3997 4156 518 Size: 648 852 579 1041 359 159 2042 Fragments arranged by size: 2042 1041 852 648 579 359 159 MspA1I CmG'CkG Cuts at: 1606 2318 3096 3773 4921 5166 1606 Size: 712 778 677 1148 245 2120 Fragments arranged by size: 2120 1148 778 712 677 245 NcoI C'CATG_G Cuts at: 513 1065 2114 513 Size: 552 1049 4079 Fragments arranged by size: 4079 1049 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5680 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5680 NotI GC'GGCC_GC Cuts at: 3127 3127 Size: 5680 NspI r_CATG'y Cuts at: 1132 1229 2126 2515 3038 4583 1132 Size: 97 897 389 523 1545 2229 Fragments arranged by size: 2229 1545 897 523 389 97 PasI CC'CwG_GG Cuts at: 1348 2852 1348 Size: 1504 4176 PciI A'CATG_T Cuts at: 4579 4579 Size: 5680 PflMI CCAn_nnn'nTGG Cuts at: 1108 2232 1108 Size: 1124 4556 PmeI GTTT'AAAC Cuts at: 2984 2984 Size: 5680 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3749 3781 5262 5294 3749 Size: 32 1481 32 4135 Fragments arranged by size: 4135 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2939 1613 Size: 1326 4354 PsiI TTA'TAA Cuts at: 3250 3250 Size: 5680 PstI C_TGCA'G Cuts at: 830 3032 830 Size: 2202 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5680 PvuII CAG'CTG Cuts at: 2318 2318 Size: 5680 SalI G'TCGA_C Cuts at: 1936 3022 1936 Size: 1086 4594 SbfI CC_TGCA'GG Cuts at: 3032 3032 Size: 5680 ScaI AGT'ACT Cuts at: 3928 5640 3928 Size: 1712 3968 SfcI C'TryA_G Cuts at: 826 1045 2876 3028 4163 4844 5035 826 Size: 219 1831 152 1135 681 191 1471 Fragments arranged by size: 1831 1471 1135 681 219 191 152 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5680 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5680 SmaI CCC'GGG Cuts at: 1872 2070 3007 1872 Size: 198 937 4545 Fragments arranged by size: 4545 937 198 SmlI C'TyrA_G Cuts at: 820 1017 1943 3784 4685 4947 820 Size: 197 926 1841 901 262 1553 Fragments arranged by size: 1841 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3471 493 Size: 2978 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5680 SphI G_CATG'C Cuts at: 1132 2126 3038 1132 Size: 994 912 3774 Fragments arranged by size: 3774 994 912 SspI AAT'ATT Cuts at: 5 52 3604 5 Size: 47 3552 2081 Fragments arranged by size: 3552 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5680 StyI C'CwwG_G Cuts at: 513 1065 2114 3119 513 Size: 552 1049 1005 3074 Fragments arranged by size: 3074 1049 1005 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2665 3707 3724 3877 4062 4507 325 Size: 1255 1085 1042 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1085 1042 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2631 3926 5638 Size: 274 80 33 51 1443 654 1295 1712 Cuts at: 5638 96 Size: 138 Fragments arranged by size: 1712 1443 1295 654 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2407 2439 5640 5672 2407 Size: 32 3201 32 2415 Fragments arranged by size: 3201 2415 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5680 XbaI T'CTAG_A Cuts at: 3016 3016 Size: 5680 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5680 XmaI C'CCGG_G Cuts at: 1870 2068 3005 1870 Size: 198 937 4545 Fragments arranged by size: 4545 937 198 XmnI GAAnn'nnTTC Cuts at: 3809 3809 Size: 5680 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3488 276 Size: 53 83 186 936 1954 2468 Fragments arranged by size: 2468 1954 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI