(Circular) (Six-base) MAPSORT of: pFN21AE4539.seq Check: 2267 from: 1 to: 6571 pFN21AE4539 6571 bp With 182 enzymes: SgfI * July 6, 2012 17:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3057 3176 3429 4381 Size: 53 83 186 936 1521 119 253 952 Cuts at: 4381 278 Size: 2468 Fragments arranged by size: 2468 1521 952 936 253 186 119 83 53 AccI GT'mk_AC Cuts at: 1937 2717 3914 1937 Size: 780 1197 4594 Fragments arranged by size: 4594 1197 780 Acc65I G'GTAC_C Cuts at: 3321 3892 3321 Size: 571 6000 AclI AA'CG_TT Cuts at: 1563 4698 5071 1563 Size: 3135 373 3063 Fragments arranged by size: 3135 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2065 2898 3209 3674 4634 6018 6286 1632 Size: 433 833 311 465 960 1384 268 1917 Fragments arranged by size: 1917 1384 960 833 465 433 311 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6374 AflIII A'CryG_T Cuts at: 1203 3402 3404 5470 6447 1203 Size: 2199 2 2066 977 1327 Fragments arranged by size: 2199 2066 1327 977 2 AgeI A'CCGG_T Cuts at: 5402 5402 Size: 6571 AhdI GACnn_n'nnGTC Cuts at: 2907 5300 2907 Size: 2393 4178 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6571 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2154 1184 Size: 970 5601 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2850 2882 2850 Size: 32 6539 AlwNI CAG_nnn'CTG Cuts at: 2488 3118 3375 3397 4314 5886 2488 Size: 630 257 22 917 1572 3173 Fragments arranged by size: 3173 1572 917 630 257 22 ApaI G_GGCC'C Cuts at: 2406 3514 2406 Size: 1108 5463 ApaLI G'TGCA_C Cuts at: 4628 5784 4628 Size: 1156 5415 ApoI r'AATT_y Cuts at: 1493 3880 4106 4258 6367 1493 Size: 2387 226 152 2109 1697 Fragments arranged by size: 2387 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5125 160 Size: 872 4093 1606 Fragments arranged by size: 4093 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6571 AvaI C'yCGr_G Cuts at: 1870 1943 2104 2339 2398 2461 2474 2638 2832 Size: 73 161 235 59 63 13 164 194 Cuts at: 2832 2930 3098 3341 3796 3896 1870 Size: 98 168 243 455 100 4545 Fragments arranged by size: 4545 455 243 235 194 168 164 161 100 98 73 63 59 13 AvrII C'CTAG_G Cuts at: 3814 3814 Size: 6571 BamHI G'GATC_C Cuts at: 3690 3901 4268 3690 Size: 211 367 5993 Fragments arranged by size: 5993 367 211 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3321 3669 3892 5347 618 Size: 325 217 627 1534 348 223 1455 1842 Fragments arranged by size: 1842 1534 1455 627 348 325 223 217 BanII G_rGCy'C Cuts at: 1391 2337 2406 2473 2557 3081 3146 3356 3514 Size: 946 69 67 84 524 65 210 158 Cuts at: 3514 3890 1391 Size: 376 4072 Fragments arranged by size: 4072 946 524 376 210 158 84 69 67 65 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2906 928 Size: 971 1007 4593 Fragments arranged by size: 4593 1007 971 BbvCI CC'TCA_GC Cuts at: 3335 3615 3776 3335 Size: 280 161 6130 Fragments arranged by size: 6130 280 161 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4762 4796 1903 Size: 34 2825 34 3678 Fragments arranged by size: 3678 2825 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4463 5673 4463 Size: 1210 5361 BclI T'GATC_A Cuts at: 1548 1548 Size: 6571 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2603 2678 2999 Size: 107 122 71 631 605 931 75 321 Cuts at: 2999 5182 136 Size: 2183 1525 Fragments arranged by size: 2183 1525 931 631 605 321 122 107 75 71 BglII A'GATC_T Cuts at: 6566 6566 Size: 6571 BlpI GC'TnA_GC Cuts at: 2534 3350 3988 2534 Size: 816 638 5117 Fragments arranged by size: 5117 816 638 Bme1580I G_kGCm'C Cuts at: 1790 2406 3219 3363 3514 4632 5788 1790 Size: 616 813 144 151 1118 1156 2573 Fragments arranged by size: 2573 1156 1118 813 616 151 144 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6571 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4302 5260 442 Size: 416 53 83 399 24 2885 958 1753 Fragments arranged by size: 2885 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6571 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2487 3370 3811 2487 Size: 883 441 5247 Fragments arranged by size: 5247 883 441 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2139 3200 5231 1239 Size: 900 1061 2031 2579 Fragments arranged by size: 2579 2031 1061 900 Bpu10I CC'TnA_GC Cuts at: 2234 3335 3615 3776 2234 Size: 1101 280 161 5029 Fragments arranged by size: 5029 1101 280 161 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3119 4696 5561 5859 3119 Size: 1577 865 298 3831 Fragments arranged by size: 3831 1577 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2532 3176 5234 882 Size: 617 1033 644 2058 2219 Fragments arranged by size: 2219 2058 1033 644 617 BsaAI yAC'GTr Cuts at: 493 1204 3405 3643 4362 493 Size: 711 2201 238 719 2702 Fragments arranged by size: 2702 2201 719 711 238 BsaBI GATnn'nnATC Cuts at: 6565 6565 Size: 6571 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2352 3054 3173 3426 Size: 53 83 186 936 819 702 119 253 Cuts at: 3426 3803 4378 4760 5395 275 Size: 377 575 382 635 1451 Fragments arranged by size: 1451 936 819 702 635 575 382 377 253 186 119 83 53 BsaWI w'CCGG_w Cuts at: 5004 5402 5676 5823 6234 5004 Size: 398 274 147 411 5341 Fragments arranged by size: 5341 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3314 3344 1178 Size: 30 165 30 1911 30 4405 Fragments arranged by size: 4405 1911 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2818 3359 3677 1186 Size: 1632 541 318 4080 Fragments arranged by size: 4080 1632 541 318 BseYI C'CCAG_C Cuts at: 2511 2767 3365 3724 3730 5774 6270 2511 Size: 256 598 359 6 2044 496 2812 Fragments arranged by size: 2812 2044 598 496 359 256 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2117 2398 3641 1163 Size: 573 381 281 1243 4093 Fragments arranged by size: 4093 1243 573 381 281 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2607 2876 3253 4021 4782 5810 Size: 275 186 606 269 377 768 761 1028 Cuts at: 5810 1540 Size: 2301 Fragments arranged by size: 2301 1028 768 761 606 377 275 269 186 BsiHKAI G_wGCw'C Cuts at: 2240 3081 3332 3356 4632 4717 5788 2240 Size: 841 251 24 1276 85 1071 3023 Fragments arranged by size: 3023 1276 1071 841 251 85 24 BsmI GAATG_Cn' Cuts at: 4082 4175 4082 Size: 93 6478 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2240 2337 2406 2473 2557 3081 3146 Size: 399 450 97 69 67 84 524 65 Cuts at: 3146 3219 3332 3356 3363 3514 3890 4632 4717 Size: 73 113 24 7 151 376 742 85 Cuts at: 4717 5788 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 524 450 399 376 151 113 97 85 84 73 69 67 65 24 7 BspEI T'CCGG_A Cuts at: 6234 6234 Size: 6571 BspHI T'CATG_A Cuts at: 3573 4460 6190 3573 Size: 887 1730 3954 Fragments arranged by size: 3954 1730 887 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2376 2463 2586 3926 844 Size: 1532 87 123 1340 3489 Fragments arranged by size: 3489 1532 1340 123 87 BsrBI CCG'CTC Cuts at: 1394 1823 3667 4458 1394 Size: 429 1844 791 3507 Fragments arranged by size: 3507 1844 791 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5066 5240 65 Size: 1183 3818 174 1396 Fragments arranged by size: 3818 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2322 5215 5402 1700 Size: 110 512 2893 187 2869 Fragments arranged by size: 2893 2869 512 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6571 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6571 BssSI C'ACGA_G Cuts at: 4631 5643 4631 Size: 1012 5559 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6571 BstBI TT'CG_AA Cuts at: 5375 5375 Size: 6571 BstEII G'GTnAC_C Cuts at: 1184 3092 1184 Size: 1908 4663 BstXI CCAn_nnnn'nTGG Cuts at: 2204 3010 2204 Size: 806 5765 BstYI r'GATC_y Cuts at: 1972 3489 3690 3901 4268 4653 4670 6122 6208 Size: 1517 201 211 367 385 17 1452 86 Cuts at: 6208 6220 6566 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1517 1452 385 367 346 211 201 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3147 3231 3147 Size: 84 6487 BtgI C'CryG_G Cuts at: 513 1065 2004 2876 3516 513 Size: 552 939 872 640 3568 Fragments arranged by size: 3568 939 872 640 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5832 Fragments arranged by size: 5832 683 56 BtsI GCAGTG_nn' Cuts at: 3845 4087 4881 4901 3845 Size: 242 794 20 5515 Fragments arranged by size: 5515 794 242 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6571 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6536 DraI TTT'AAA Cuts at: 3875 3875 Size: 6571 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5578 809 Size: 507 4262 1802 Fragments arranged by size: 4262 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2160 2595 2604 Size: 54 1425 50 275 18 330 435 9 Cuts at: 2604 2808 2873 3000 3237 3564 3867 4018 4907 Size: 204 65 127 237 327 303 151 889 Cuts at: 4907 6536 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 435 330 327 303 275 237 204 151 127 65 54 50 43 18 9 EagI C'GGCC_G Cuts at: 1537 1812 2604 2873 4018 1537 Size: 275 792 269 1145 4090 Fragments arranged by size: 4090 1145 792 275 269 EarI CTCTTCn'nnn_ Cuts at: 1353 2084 3134 4501 1353 Size: 731 1050 1367 3423 Fragments arranged by size: 3423 1367 1050 731 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3674 5158 5532 5678 387 Size: 1064 138 2085 1484 374 146 1280 Fragments arranged by size: 2085 1484 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3079 3354 3079 Size: 275 6296 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2065 2139 2898 3200 3209 3674 4634 Size: 393 433 74 759 302 9 465 960 Cuts at: 4634 5231 6018 6286 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 960 787 759 597 465 433 393 302 268 74 9 EcoNI CCTnn'n_nnAGG Cuts at: 2651 2651 Size: 6571 EcoO109I rG'GnC_Cy Cuts at: 2037 2046 2109 2344 2402 2403 2642 2696 2752 Size: 9 63 235 58 1 239 54 56 Cuts at: 2752 2836 3387 3394 3510 3511 3635 3649 3790 Size: 84 551 7 116 1 124 14 141 Cuts at: 3790 3818 2037 Size: 28 4790 Fragments arranged by size: 4790 551 239 235 141 124 116 84 63 58 56 54 28 14 9 7 1 1 EcoRI G'AATT_C Cuts at: 3880 4258 3880 Size: 378 6193 FspI TGC'GCA Cuts at: 5077 5077 Size: 6571 HaeII r_GCGC'y Cuts at: 1678 2590 3536 5718 1678 Size: 912 946 2182 2531 Fragments arranged by size: 2531 2182 946 912 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2381 2413 2728 2760 2893 Size: 32 135 32 809 32 315 32 133 Cuts at: 2893 2925 3816 3848 5212 5244 5286 5318 1373 Size: 32 891 32 1364 32 42 32 2626 Fragments arranged by size: 2626 1364 891 809 315 135 133 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2718 3208 3606 3915 4161 669 Size: 988 281 780 490 398 309 246 3079 Fragments arranged by size: 3079 988 780 490 398 309 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6571 HpaI GTT'AAC Cuts at: 4161 4161 Size: 6571 KpnI G_GTAC'C Cuts at: 3325 3896 3325 Size: 571 6000 MfeI C'AATT_G Cuts at: 4170 4170 Size: 6571 MluI A'CGCG_T Cuts at: 6447 6447 Size: 6571 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5685 5869 1305 Size: 4380 184 2007 Fragments arranged by size: 4380 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2162 3002 3239 3566 10 Size: 54 1425 343 330 840 237 327 3015 Fragments arranged by size: 3015 1425 840 343 330 327 237 54 MslI CAynn'nnrTG Cuts at: 518 1166 3008 3572 4529 4888 5047 518 Size: 648 1842 564 957 359 159 2042 Fragments arranged by size: 2042 1842 957 648 564 359 159 MspA1I CmG'CkG Cuts at: 1606 2305 2485 2878 3049 3421 3703 3987 4664 Size: 699 180 393 171 372 282 284 677 Cuts at: 4664 5812 6057 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 699 677 393 372 284 282 245 180 171 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5080 Fragments arranged by size: 5080 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6571 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6571 NotI GC'GGCC_GC Cuts at: 4018 4018 Size: 6571 NspI r_CATG'y Cuts at: 1132 1229 3784 3929 5474 1132 Size: 97 2555 145 1545 2229 Fragments arranged by size: 2555 2229 1545 145 97 PasI CC'CwG_GG Cuts at: 1348 3317 1348 Size: 1969 4602 PciI A'CATG_T Cuts at: 5470 5470 Size: 6571 PflMI CCAn_nnn'nTGG Cuts at: 1108 3118 3397 1108 Size: 2010 279 4282 Fragments arranged by size: 4282 2010 279 PfoI T'CCnGG_A Cuts at: 2250 2250 Size: 6571 PmeI GTTT'AAAC Cuts at: 3875 3875 Size: 6571 PmlI CAC'GTG Cuts at: 3405 3643 3405 Size: 238 6333 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4640 4672 6153 6185 4640 Size: 32 1481 32 5026 Fragments arranged by size: 5026 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2037 2046 2109 2344 2642 2696 2836 3394 3635 Size: 9 63 235 298 54 140 558 241 Cuts at: 3635 3818 2037 Size: 183 4790 Fragments arranged by size: 4790 558 298 241 235 183 140 63 54 9 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6571 PsiI TTA'TAA Cuts at: 4141 4141 Size: 6571 PspOMI G'GGCC_C Cuts at: 2402 3510 2402 Size: 1108 5463 PspXI vC'TCGA_Gb Cuts at: 2104 2104 Size: 6571 PstI C_TGCA'G Cuts at: 830 2688 3923 830 Size: 1858 1235 3478 Fragments arranged by size: 3478 1858 1235 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6571 PvuII CAG'CTG Cuts at: 2305 3049 3421 2305 Size: 744 372 5455 Fragments arranged by size: 5455 744 372 SacI G_AGCT'C Cuts at: 3081 3356 3081 Size: 275 6296 SacII CC_GC'GG Cuts at: 2879 2879 Size: 6571 SalI G'TCGA_C Cuts at: 1936 2716 3913 1936 Size: 780 1197 4594 Fragments arranged by size: 4594 1197 780 SanDI GG'GwC_CC Cuts at: 2046 2642 2696 2836 3635 2046 Size: 596 54 140 799 4982 Fragments arranged by size: 4982 799 596 140 54 SapI GCTCTTCn'nnn_ Cuts at: 2084 2084 Size: 6571 SbfI CC_TGCA'GG Cuts at: 3923 3923 Size: 6571 ScaI AGT'ACT Cuts at: 4819 6531 4819 Size: 1712 4859 SexAI A'CCwGG_T Cuts at: 3762 3762 Size: 6571 SfcI C'TryA_G Cuts at: 826 1045 2684 3737 3919 5054 5735 5926 826 Size: 219 1639 1053 182 1135 681 191 1471 Fragments arranged by size: 1639 1471 1135 1053 681 219 191 182 SfiI GGCCn_nnn'nGGCC Cuts at: 2603 2999 2603 Size: 396 6175 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6571 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6571 SmaI CCC'GGG Cuts at: 1872 2640 3898 1872 Size: 768 1258 4545 Fragments arranged by size: 4545 1258 768 SmlI C'TyrA_G Cuts at: 820 1017 1943 2104 3134 4675 5576 5838 820 Size: 197 926 161 1030 1541 901 262 1553 Fragments arranged by size: 1553 1541 1030 926 901 262 197 161 SnaBI TAC'GTA Cuts at: 493 4362 493 Size: 3869 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6571 SphI G_CATG'C Cuts at: 1132 3784 3929 1132 Size: 2652 145 3774 Fragments arranged by size: 3774 2652 145 SspI AAT'ATT Cuts at: 5 52 4495 5 Size: 47 4443 2081 Fragments arranged by size: 4443 2081 47 StuI AGG'CCT Cuts at: 1517 2411 2565 1517 Size: 894 154 5523 Fragments arranged by size: 5523 894 154 StyI C'CwwG_G Cuts at: 513 1065 2004 2203 2268 3413 3438 3814 4010 Size: 552 939 199 65 1145 25 376 196 Cuts at: 4010 513 Size: 3074 Fragments arranged by size: 3074 1145 939 552 376 199 196 65 25 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2083 3020 3494 4598 4615 4768 4953 Size: 1255 503 937 474 1104 17 153 185 Cuts at: 4953 5398 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1104 937 503 474 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4817 6529 96 Size: 274 80 33 51 1443 2840 1712 138 Fragments arranged by size: 2840 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3627 3659 6531 6563 3627 Size: 32 2872 32 3635 Fragments arranged by size: 3635 2872 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2153 2722 1316 Size: 837 569 5165 Fragments arranged by size: 5165 837 569 XbaI T'CTAG_A Cuts at: 3907 3907 Size: 6571 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3760 3760 Size: 6571 XhoI C'TCGA_G Cuts at: 1943 2104 1943 Size: 161 6410 XmaI C'CCGG_G Cuts at: 1870 2638 3896 1870 Size: 768 1258 4545 Fragments arranged by size: 4545 1258 768 XmnI GAAnn'nnTTC Cuts at: 4700 4700 Size: 6571 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3055 3174 3427 4379 Size: 53 83 186 936 1521 119 253 952 Cuts at: 4379 276 Size: 2468 Fragments arranged by size: 2468 1521 952 936 253 186 119 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PsrI RsrII SfoI SrfI SwaI