(Circular) (Six-base) MAPSORT of: pFN21AE4435.seq Check: 947 from: 1 to: 6061 pFN21AE4435 6061 bp With 182 enzymes: SgfI * March 28, 2012 13:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2999 3871 278 Size: 53 83 186 936 1463 872 2468 Fragments arranged by size: 2468 1463 936 872 186 83 53 AccI GT'mk_AC Cuts at: 1937 3404 1937 Size: 1467 4594 Acc65I G'GTAC_C Cuts at: 3382 3382 Size: 6061 AclI AA'CG_TT Cuts at: 1563 4188 4561 1563 Size: 2625 373 3063 Fragments arranged by size: 3063 2625 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2408 4124 5508 5776 1632 Size: 776 1716 1384 268 1917 Fragments arranged by size: 1917 1716 1384 776 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5864 AflIII A'CryG_T Cuts at: 1203 3131 4960 5937 1203 Size: 1928 1829 977 1327 Fragments arranged by size: 1928 1829 1327 977 AgeI A'CCGG_T Cuts at: 4892 4892 Size: 6061 AhdI GACnn_n'nnGTC Cuts at: 4790 4790 Size: 6061 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6061 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6061 AlwNI CAG_nnn'CTG Cuts at: 3196 3804 5376 3196 Size: 608 1572 3881 Fragments arranged by size: 3881 1572 608 ApaLI G'TGCA_C Cuts at: 4118 5274 4118 Size: 1156 4905 ApoI r'AATT_y Cuts at: 1493 3370 3596 3748 5857 1493 Size: 1877 226 152 2109 1697 Fragments arranged by size: 2109 1877 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4615 160 Size: 872 3583 1606 Fragments arranged by size: 3583 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 AvaI C'yCGr_G Cuts at: 1870 1943 2255 3386 1870 Size: 73 312 1131 4545 Fragments arranged by size: 4545 1131 312 73 BamHI G'GATC_C Cuts at: 3391 3758 3391 Size: 367 5694 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2068 2112 3382 4837 618 Size: 325 217 627 281 44 1270 1455 1842 Fragments arranged by size: 1842 1455 1270 627 325 281 217 44 BanII G_rGCy'C Cuts at: 1391 2262 2457 3349 3380 1391 Size: 871 195 892 31 4072 Fragments arranged by size: 4072 892 871 195 31 BbeI G_GCGC'C Cuts at: 2072 2116 2072 Size: 44 6017 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2437 2581 2895 3029 928 Size: 971 538 144 314 134 3960 Fragments arranged by size: 3960 971 538 314 144 134 BbvCI CC'TCA_GC Cuts at: 3036 3269 3036 Size: 233 5828 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4252 4286 1903 Size: 34 2315 34 3678 Fragments arranged by size: 3678 2315 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3953 5163 3953 Size: 1210 4851 BclI T'GATC_A Cuts at: 1548 1548 Size: 6061 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4672 136 Size: 107 122 71 631 605 3000 1525 Fragments arranged by size: 3000 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2808 2850 6056 2808 Size: 42 3206 2813 Fragments arranged by size: 3206 2813 42 BlpI GC'TnA_GC Cuts at: 3478 3478 Size: 6061 Bme1580I G_kGCm'C Cuts at: 1790 4122 5278 1790 Size: 2332 1156 2573 Fragments arranged by size: 2573 2332 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6061 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3792 4750 442 Size: 416 53 83 399 24 2375 958 1753 Fragments arranged by size: 2375 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6061 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2704 2704 Size: 6061 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3161 3218 4721 1239 Size: 1922 57 1503 2579 Fragments arranged by size: 2579 1922 1503 57 Bpu10I CC'TnA_GC Cuts at: 2889 3036 3269 2889 Size: 147 233 5681 Fragments arranged by size: 5681 233 147 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2882 4186 5051 5349 2882 Size: 1304 865 298 3594 Fragments arranged by size: 3594 1304 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4724 882 Size: 617 3225 2219 Fragments arranged by size: 3225 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2227 3134 3852 493 Size: 711 1023 907 718 2702 Fragments arranged by size: 2702 1023 907 718 711 BsaBI GATnn'nnATC Cuts at: 6055 6055 Size: 6061 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2069 2113 2996 3868 Size: 53 83 186 936 536 44 883 872 Cuts at: 3868 4250 4885 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 883 872 635 536 382 186 83 53 44 BsaWI w'CCGG_w Cuts at: 2421 4494 4892 5166 5313 5724 2421 Size: 2073 398 274 147 411 2758 Fragments arranged by size: 2758 2073 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3191 3221 1178 Size: 30 165 30 1788 30 4018 Fragments arranged by size: 4018 1788 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6061 BseYI C'CCAG_C Cuts at: 5264 5760 5264 Size: 496 5565 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2289 2969 1163 Size: 573 553 680 4255 Fragments arranged by size: 4255 680 573 553 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2060 3511 4272 5300 1540 Size: 275 186 59 1451 761 1028 2301 Fragments arranged by size: 2301 1451 1028 761 275 186 59 BsiHKAI G_wGCw'C Cuts at: 2753 2865 3078 3147 3294 4122 4207 5278 2753 Size: 112 213 69 147 828 85 1071 3536 Fragments arranged by size: 3536 1071 828 213 147 112 85 69 BsmI GAATG_Cn' Cuts at: 3258 3572 3665 3258 Size: 314 93 5654 Fragments arranged by size: 5654 314 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3209 3209 Size: 6061 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2262 2457 2753 2865 3078 3147 3294 Size: 399 472 195 296 112 213 69 147 Cuts at: 3294 3349 3380 4122 4207 5278 1391 Size: 55 31 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 472 399 296 213 195 147 112 85 69 55 31 BspEI T'CCGG_A Cuts at: 2421 5724 2421 Size: 3303 2758 BspHI T'CATG_A Cuts at: 3950 5680 3950 Size: 1730 4331 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2025 3349 3416 844 Size: 1181 1324 67 3489 Fragments arranged by size: 3489 1324 1181 67 BsrBI CCG'CTC Cuts at: 1394 1823 3044 3948 1394 Size: 429 1221 904 3507 Fragments arranged by size: 3507 1221 904 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3280 4556 4730 65 Size: 1183 2032 1276 174 1396 Fragments arranged by size: 2032 1396 1276 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2009 2059 2071 2570 2865 3087 4705 Size: 110 199 50 12 499 295 222 1618 Cuts at: 4705 4892 1700 Size: 187 2869 Fragments arranged by size: 2869 1618 499 295 222 199 187 110 50 12 BsrGI T'GTAC_A Cuts at: 96 2631 2667 2742 96 Size: 2535 36 75 3415 Fragments arranged by size: 3415 2535 75 36 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6061 BssSI C'ACGA_G Cuts at: 4121 5133 4121 Size: 1012 5049 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6061 BstBI TT'CG_AA Cuts at: 4865 4865 Size: 6061 BstEII G'GTnAC_C Cuts at: 1184 2290 1184 Size: 1106 4955 BstXI CCAn_nnnn'nTGG Cuts at: 2975 3247 2975 Size: 272 5789 BstYI r'GATC_y Cuts at: 1972 2808 2850 3391 3758 4143 4160 5612 5698 Size: 836 42 541 367 385 17 1452 86 Cuts at: 5698 5710 6056 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 836 541 385 367 346 86 42 17 12 Bsu36I CC'TnA_GG Cuts at: 2047 2047 Size: 6061 BtgI C'CryG_G Cuts at: 513 1065 2004 2108 2151 2527 3306 513 Size: 552 939 104 43 376 779 3268 Fragments arranged by size: 3268 939 779 552 376 104 43 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3094 487 Size: 683 56 1868 3454 Fragments arranged by size: 3454 1868 683 56 BtsI GCAGTG_nn' Cuts at: 2614 3577 4371 4391 2614 Size: 963 794 20 4284 Fragments arranged by size: 4284 963 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6061 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2536 2571 529 Size: 35 1972 35 4019 Fragments arranged by size: 4019 1972 35 35 DraI TTT'AAA Cuts at: 3365 3365 Size: 6061 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5068 809 Size: 507 3752 1802 Fragments arranged by size: 3752 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2057 2310 Size: 54 1425 50 275 18 177 50 253 Cuts at: 2310 2735 3508 4397 6026 8 Size: 425 773 889 1629 43 Fragments arranged by size: 1629 1425 889 773 425 275 253 177 54 50 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2057 3508 1537 Size: 275 245 1451 4090 Fragments arranged by size: 4090 1451 275 245 EarI CTCTTCn'nnn_ Cuts at: 1353 2370 3991 1353 Size: 1017 1621 3423 Fragments arranged by size: 3423 1621 1017 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4648 5022 5168 387 Size: 1064 138 3059 374 146 1280 Fragments arranged by size: 3059 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2408 3161 3218 4124 4721 5508 5776 Size: 393 776 753 57 906 597 787 268 Cuts at: 5776 1239 Size: 1524 Fragments arranged by size: 1524 906 787 776 753 597 393 268 57 EcoNI CCTnn'n_nnAGG Cuts at: 2966 2966 Size: 6061 EcoO109I rG'GnC_Cy Cuts at: 2893 3244 2893 Size: 351 5710 EcoRI G'AATT_C Cuts at: 3370 3748 3370 Size: 378 5683 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2815 2851 2815 Size: 36 6025 FseI GG_CCGG'CC Cuts at: 2063 3091 2063 Size: 1028 5033 FspI TGC'GCA Cuts at: 2700 4567 2700 Size: 1867 4194 HaeII r_GCGC'y Cuts at: 1678 2072 2116 5208 1678 Size: 394 44 3092 2531 Fragments arranged by size: 3092 2531 394 44 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2936 2968 4702 4734 4776 Size: 32 135 32 1364 32 1734 32 42 Cuts at: 4776 4808 1373 Size: 32 2626 Fragments arranged by size: 2626 1734 1364 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3405 3651 669 Size: 988 281 1467 246 3079 Fragments arranged by size: 3079 1467 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6061 HpaI GTT'AAC Cuts at: 3651 3651 Size: 6061 KasI G'GCGC_C Cuts at: 2068 2112 2068 Size: 44 6017 KpnI G_GTAC'C Cuts at: 3386 3386 Size: 6061 MfeI C'AATT_G Cuts at: 3660 3660 Size: 6061 MluI A'CGCG_T Cuts at: 5937 5937 Size: 6061 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5175 5359 1305 Size: 3870 184 2007 Fragments arranged by size: 3870 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2312 10 Size: 54 1425 343 480 3759 Fragments arranged by size: 3759 1425 480 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2526 2663 4019 4378 4537 518 Size: 648 1360 137 1356 359 159 2042 Fragments arranged by size: 2042 1360 1356 648 359 159 137 MspA1I CmG'CkG Cuts at: 1606 2110 2706 2740 3477 4154 5302 5547 1606 Size: 504 596 34 737 677 1148 245 2120 Fragments arranged by size: 2120 1148 737 677 596 504 245 34 NaeI GCC'GGC Cuts at: 2011 2061 2073 2572 3089 2011 Size: 50 12 499 517 4983 Fragments arranged by size: 4983 517 499 50 12 NarI GG'CG_CC Cuts at: 2069 2113 2069 Size: 44 6017 NcoI C'CATG_G Cuts at: 513 1065 2004 2527 513 Size: 552 939 523 4047 Fragments arranged by size: 4047 939 552 523 NdeI CA'TA_TG Cuts at: 387 387 Size: 6061 NgoMIV G'CCGG_C Cuts at: 2009 2059 2071 2570 3087 2009 Size: 50 12 499 517 4983 Fragments arranged by size: 4983 517 499 50 12 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6061 NotI GC'GGCC_GC Cuts at: 3508 3508 Size: 6061 NspI r_CATG'y Cuts at: 1132 1229 2668 3419 4964 1132 Size: 97 1439 751 1545 2229 Fragments arranged by size: 2229 1545 1439 751 97 PasI CC'CwG_GG Cuts at: 1348 2968 3349 1348 Size: 1620 381 4060 Fragments arranged by size: 4060 1620 381 PciI A'CATG_T Cuts at: 4960 4960 Size: 6061 PflMI CCAn_nnn'nTGG Cuts at: 1108 2603 3196 1108 Size: 1495 593 3973 Fragments arranged by size: 3973 1495 593 PfoI T'CCnGG_A Cuts at: 2448 2802 2448 Size: 354 5707 PmeI GTTT'AAAC Cuts at: 3365 3365 Size: 6061 PmlI CAC'GTG Cuts at: 2227 3134 2227 Size: 907 5154 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4130 4162 5643 5675 4130 Size: 32 1481 32 4516 Fragments arranged by size: 4516 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2893 3244 2893 Size: 351 5710 PshAI GACnn'nnGTC Cuts at: 1613 2999 1613 Size: 1386 4675 PsiI TTA'TAA Cuts at: 3631 3631 Size: 6061 PstI C_TGCA'G Cuts at: 830 2042 3413 830 Size: 1212 1371 3478 Fragments arranged by size: 3478 1371 1212 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6061 PvuII CAG'CTG Cuts at: 2706 2706 Size: 6061 SacII CC_GC'GG Cuts at: 2111 2111 Size: 6061 SalI G'TCGA_C Cuts at: 1936 3403 1936 Size: 1467 4594 SbfI CC_TGCA'GG Cuts at: 3413 3413 Size: 6061 ScaI AGT'ACT Cuts at: 4309 6021 4309 Size: 1712 4349 SexAI A'CCwGG_T Cuts at: 2286 2286 Size: 6061 SfcI C'TryA_G Cuts at: 826 1045 2038 3409 4544 5225 5416 826 Size: 219 993 1371 1135 681 191 1471 Fragments arranged by size: 1471 1371 1135 993 681 219 191 SfoI GGC'GCC Cuts at: 2070 2114 2070 Size: 44 6017 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 SgrAI Cr'CCGG_yG Cuts at: 1700 2071 1700 Size: 371 5690 SmaI CCC'GGG Cuts at: 1872 3388 1872 Size: 1516 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2897 4165 5066 5328 820 Size: 197 926 954 1268 901 262 1553 Fragments arranged by size: 1553 1268 954 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3852 493 Size: 3359 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6061 SphI G_CATG'C Cuts at: 1132 3419 1132 Size: 2287 3774 SspI AAT'ATT Cuts at: 5 52 3985 5 Size: 47 3933 2081 Fragments arranged by size: 3933 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6061 StyI C'CwwG_G Cuts at: 513 1065 2004 2527 2983 3240 3500 513 Size: 552 939 523 456 257 260 3074 Fragments arranged by size: 3074 939 552 523 456 260 257 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2330 2846 4088 4105 4258 4443 4888 Size: 1255 750 516 1242 17 153 185 445 Cuts at: 4888 325 Size: 1498 Fragments arranged by size: 1498 1255 1242 750 516 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2322 2538 2631 Size: 274 80 33 51 1443 345 216 93 Cuts at: 2631 2667 2742 4307 6019 96 Size: 36 75 1565 1712 138 Fragments arranged by size: 1712 1565 1443 345 274 216 138 93 80 75 51 36 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2469 2501 3191 3223 6021 6053 2469 Size: 32 690 32 2798 32 2477 Fragments arranged by size: 2798 2477 690 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2288 1316 Size: 972 5089 XbaI T'CTAG_A Cuts at: 3397 3397 Size: 6061 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2554 2554 Size: 6061 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6061 XmaI C'CCGG_G Cuts at: 1870 3386 1870 Size: 1516 4545 XmnI GAAnn'nnTTC Cuts at: 4190 4190 Size: 6061 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2997 3869 276 Size: 53 83 186 936 1463 872 2468 Fragments arranged by size: 2468 1463 936 872 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoICRI EcoRV FspAI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SanDI SapI SfiI SrfI SwaI