(Linear) (Six-base) MAPSORT of: KIEE4576.seq Check: 9890 from: 1 to: 2289 KIEE4576.orf With 182 enzymes: SgfI * March 22, 2012 18:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 289 2097 2289 Size: 289 1808 192 Fragments arranged by size: 1808 289 192 Acc65I G'GTAC_C Cuts at: 0 508 2289 Size: 508 1781 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 30 536 1260 1553 1688 1996 2015 2289 Size: 30 506 724 293 135 308 19 274 Fragments arranged by size: 724 506 308 293 274 135 30 19 AflII C'TTAA_G Cuts at: 0 1360 2289 Size: 1360 929 AflIII A'CryG_T Cuts at: 0 314 1791 2289 Size: 314 1477 498 Fragments arranged by size: 1477 498 314 AleI CACnn'nnGTG Cuts at: 0 1759 2289 Size: 1759 530 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2076 2289 Size: 2076 213 AlwNI CAG_nnn'CTG Cuts at: 0 304 1041 1524 2289 Size: 304 737 483 765 Fragments arranged by size: 765 737 483 304 ApoI r'AATT_y Cuts at: 0 976 1057 1944 2289 Size: 976 81 887 345 Fragments arranged by size: 976 887 345 81 AvaI C'yCGr_G Cuts at: 0 62 2289 Size: 62 2227 AvrII C'CTAG_G Cuts at: 0 1881 2289 Size: 1881 408 BamHI G'GATC_C Cuts at: 0 50 427 2289 Size: 50 377 1862 Fragments arranged by size: 1862 377 50 BanI G'GyrC_C Cuts at: 0 508 1747 2173 2289 Size: 508 1239 426 116 Fragments arranged by size: 1239 508 426 116 BanII G_rGCy'C Cuts at: 0 1068 2289 Size: 1068 1221 BbeI G_GCGC'C Cuts at: 0 2177 2289 Size: 2177 112 BbsI GAAGACnn'nnnn_ Cuts at: 0 86 2018 2289 Size: 86 1932 271 Fragments arranged by size: 1932 271 86 BbvCI CC'TCA_GC Cuts at: 0 28 2289 Size: 28 2261 BclI T'GATC_A Cuts at: 0 246 2289 Size: 246 2043 BglI GCCn_nnn'nGGC Cuts at: 0 13 2289 Size: 13 2276 BglII A'GATC_T Cuts at: 0 764 1803 2289 Size: 764 1039 486 Fragments arranged by size: 1039 764 486 BmrI ACTGGGnnnn_n' Cuts at: 0 1298 2289 Size: 1298 991 BmtI G_CTAG'C Cuts at: 0 2251 2289 Size: 2251 38 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 677 1546 1669 2289 Size: 677 869 123 620 Fragments arranged by size: 869 677 620 123 Bpu10I CC'TnA_GC Cuts at: 0 28 2289 Size: 28 2261 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 469 1573 2182 2289 Size: 469 1104 609 107 Fragments arranged by size: 1104 609 469 107 BsaI GGTCTCn'nnnn_ Cuts at: 0 1591 2289 Size: 1591 698 BsaBI GATnn'nnATC Cuts at: 0 22 2289 Size: 22 2267 BsaHI Gr'CG_yC Cuts at: 0 2174 2289 Size: 2174 115 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 974 1004 2289 Size: 974 30 1285 Fragments arranged by size: 1285 974 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 455 2289 Size: 455 1834 BseYI C'CCAG_C Cuts at: 0 525 2289 Size: 525 1764 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 644 881 1124 2289 Size: 644 237 243 1165 Fragments arranged by size: 1165 644 243 237 BsiEI CG_ry'CG Cuts at: 0 1541 2289 Size: 1541 748 BsiHKAI G_wGCw'C Cuts at: 0 1068 2289 Size: 1068 1221 BsmI GAATG_Cn' Cuts at: 0 617 2289 Size: 617 1672 Bsp1286I G_dGCh'C Cuts at: 0 1068 2289 Size: 1068 1221 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 289 2097 2289 Size: 289 1808 192 Fragments arranged by size: 1808 289 192 BsrBI CCG'CTC Cuts at: 0 59 1827 2289 Size: 59 1768 462 Fragments arranged by size: 1768 462 59 BsrFI r'CCGG_y Cuts at: 0 952 2192 2289 Size: 952 1240 97 Fragments arranged by size: 1240 952 97 BstAPI GCAn_nnn'nTGC Cuts at: 0 1371 2289 Size: 1371 918 BstBI TT'CG_AA Cuts at: 0 156 2289 Size: 156 2133 BstXI CCAn_nnnn'nTGG Cuts at: 0 573 1761 2289 Size: 573 1188 528 Fragments arranged by size: 1188 573 528 BstYI r'GATC_y Cuts at: 0 50 427 764 1803 2075 2163 2289 Size: 50 377 337 1039 272 88 126 Fragments arranged by size: 1039 377 337 272 126 88 50 BtgI C'CryG_G Cuts at: 0 1029 1139 1505 2289 Size: 1029 110 366 784 Fragments arranged by size: 1029 784 366 110 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1110 2289 Size: 1110 1179 BtsI GCAGTG_nn' Cuts at: 0 1181 2289 Size: 1181 1108 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1713 1748 2289 Size: 1713 35 541 Fragments arranged by size: 1713 541 35 DraIII CAC_nnn'GTG Cuts at: 0 1371 2289 Size: 1371 918 EaeI y'GGCC_r Cuts at: 0 2081 2289 Size: 2081 208 EarI CTCTTCn'nnn_ Cuts at: 0 1924 2289 Size: 1924 365 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1813 2014 2289 Size: 1813 201 275 Fragments arranged by size: 1813 275 201 EcoICRI GAG'CTC Cuts at: 0 1066 2289 Size: 1066 1223 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 30 536 677 1260 1546 1553 1669 1688 Size: 30 506 141 583 286 7 116 19 Cuts at: 1688 1996 2015 2289 Size: 308 19 274 Fragments arranged by size: 583 506 308 286 274 141 116 30 19 19 7 EcoNI CCTnn'n_nnAGG Cuts at: 0 2068 2289 Size: 2068 221 EcoO109I rG'GnC_Cy Cuts at: 0 530 1038 1350 2146 2289 Size: 530 508 312 796 143 Fragments arranged by size: 796 530 508 312 143 EcoRI G'AATT_C Cuts at: 0 1944 2289 Size: 1944 345 EcoRV GAT'ATC Cuts at: 0 309 2289 Size: 309 1980 HaeII r_GCGC'y Cuts at: 0 2177 2289 Size: 2177 112 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1109 1141 1386 1418 2289 Size: 1109 32 245 32 871 Fragments arranged by size: 1109 871 245 32 32 HincII GTy'rAC Cuts at: 0 654 1698 2289 Size: 654 1044 591 Fragments arranged by size: 1044 654 591 HindIII A'AGCT_T Cuts at: 0 1728 2289 Size: 1728 561 KasI G'GCGC_C Cuts at: 0 2173 2289 Size: 2173 116 KpnI G_GTAC'C Cuts at: 0 512 2289 Size: 512 1777 MscI TGG'CCA Cuts at: 0 2083 2289 Size: 2083 206 MslI CAynn'nnrTG Cuts at: 0 1609 1759 2289 Size: 1609 150 530 Fragments arranged by size: 1609 530 150 MspA1I CmG'CkG Cuts at: 0 1521 1573 2244 2289 Size: 1521 52 671 45 Fragments arranged by size: 1521 671 52 45 NarI GG'CG_CC Cuts at: 0 2174 2289 Size: 2174 115 NcoI C'CATG_G Cuts at: 0 1029 2289 Size: 1029 1260 NheI G'CTAG_C Cuts at: 0 2247 2289 Size: 2247 42 NspI r_CATG'y Cuts at: 0 318 1491 1795 2289 Size: 318 1173 304 494 Fragments arranged by size: 1173 494 318 304 PasI CC'CwG_GG Cuts at: 0 672 2289 Size: 672 1617 PciI A'CATG_T Cuts at: 0 314 1791 2289 Size: 314 1477 498 Fragments arranged by size: 1477 498 314 PflMI CCAn_nnn'nTGG Cuts at: 0 663 2289 Size: 663 1626 PfoI T'CCnGG_A Cuts at: 0 1685 2289 Size: 1685 604 PpuMI rG'GwC_Cy Cuts at: 0 1038 1350 2289 Size: 1038 312 939 Fragments arranged by size: 1038 939 312 PshAI GACnn'nnGTC Cuts at: 0 1355 2289 Size: 1355 934 PstI C_TGCA'G Cuts at: 0 2094 2289 Size: 2094 195 PvuII CAG'CTG Cuts at: 0 1521 1573 2244 2289 Size: 1521 52 671 45 Fragments arranged by size: 1521 671 52 45 SacI G_AGCT'C Cuts at: 0 1068 2289 Size: 1068 1221 SexAI A'CCwGG_T Cuts at: 0 1642 2289 Size: 1642 647 SfcI C'TryA_G Cuts at: 0 1574 2090 2289 Size: 1574 516 199 Fragments arranged by size: 1574 516 199 SfoI GGC'GCC Cuts at: 0 2175 2289 Size: 2175 114 SmlI C'TyrA_G Cuts at: 0 448 1360 1588 2197 2289 Size: 448 912 228 609 92 Fragments arranged by size: 912 609 448 228 92 SphI G_CATG'C Cuts at: 0 1491 2289 Size: 1491 798 StuI AGG'CCT Cuts at: 0 887 1012 2289 Size: 887 125 1277 Fragments arranged by size: 1277 887 125 StyI C'CwwG_G Cuts at: 0 1029 1305 1871 1881 2289 Size: 1029 276 566 10 408 Fragments arranged by size: 1029 566 408 276 10 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 565 1208 1555 1735 2289 Size: 565 643 347 180 554 Fragments arranged by size: 643 565 554 347 180 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 124 2289 Size: 124 2165 Enzymes that do cut: AarI Acc65I AcuI AflII AflIII AleI AlfI AlwNI ApoI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BclI BglI BglII BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI CspCI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I HincII HindIII KasI KpnI MscI MslI MspA1I NarI NcoI NheI NspI PasI PciI PflMI PfoI PpuMI PshAI PstI PvuII SacI SexAI SfcI SfoI SmlI SphI StuI StyI TaqII XcmI Enzymes that do not cut: AatII AccI AclI AfeI AgeI AhdI AloI ApaI ApaLI AscI AseI AsiSI BaeI BcgI BciVI BfrBI BlpI Bme1580I BmgBI BplI BsaAI BsaWI BsiWI BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI BstEII BstZ17I Bsu36I ClaI DraI DrdI EagI FalI FseI FspI FspAI HpaI MfeI MluI MmeI NaeI NdeI NgoMIV NotI NruI NsiI PacI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI