################################## # NO CUT ENZYMES ( 94 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI Alw44I ApaLI ApoI AscI AseI AsnI Asp718I AviII AvrII BcgI BclI BfrI BglII BlnI BsaBI BsaI BsaWI BseAI BsiWI BsmI BspDI BspEI BspHI BsrDI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII DrdI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI KspI MamI MfeI MluI MroI MseI MunI NaeI NcoI NdeI NgoMIV NheI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SfuI SgrAI SnaBI SpeI SphI SspI SwaI TfiI Tru9I Tsp509I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AhdI 1640 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1056 CCTTAGG CC^T[ACGT]AGG Asp700I 306 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1640 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbrPI 1303 CACGTG CAC^GTG BciVI 160 GTATCC GTATCC(6/5) BfaI 1150 CTAG C^TAG BsaAI 1303 CACGTG [CT]AC^GT[GA] Bsp1407I 674 TGTACA T^GTACA BsrBI 1066 GAGCGG CCGCTC(-3/-3) BsrGI 674 TGTACA T^GTACA BssHII 1624 GCGCGC G^CGCGC BssSI 1867 CTCGTG CACGAG(-5/-1) BstAPI 1466 GCAGCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 1056 CCTTAGG CC^T[ACGT]AGG Eam1105I 1640 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 601 CTCTTC CTCTTC(1/4) Eco81I 1056 CCTTAGG CC^T[ACGT]AGG HincII 1609 GTCAAC GT[CT]^[GA]AC HindII 1609 GTCAAC GT[CT]^[GA]AC HinfI 1687 GACTC G^A[ACGT]TC Ksp632I 601 CTCTTC CTCTTC(1/4) MaeI 1150 CTAG C^TAG NotI 1632 GCGGCCGC GC^GGCCGC PleI 1687 GACTC GAGTC(4/5) PmaCI 1303 CACGTG CAC^GTG PmlI 1303 CACGTG CAC^GTG SapI 600 GCTCTTC GCTCTTC(1/4) SexAI 1643 ACCAGGT A^CC[AT]GGT SfiI 804 GGCCATAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 1330 CTCAAG C^T[CT][GA]AG SspBI 674 TGTACA T^GTACA XmnI 306 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AflIII 701 ACATGT A^C[GA][CT]GT 2333 ACATGT ApaI 1541 GGGCCC GGGCC^C 2308 GGGCCC AspI 1753 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC 1945 GACAATGTC BamHI 385 GGATCC G^GATCC 1289 GGATCC BsiEI 1584 CGGCCG CG[GA][CT]^CG 1633 CGGCCG BspLU11I 701 ACATGT A^CATGT 2333 ACATGT BsrFI 443 ACCGGC [GA]^CCGG[CT] 1396 ACCGGC Cfr10I 443 ACCGGC [GA]^CCGG[CT] 1396 ACCGGC EagI 1584 CGGCCG C^GGCCG 1633 CGGCCG EclXI 1584 CGGCCG C^GGCCG 1633 CGGCCG Eco52I 1584 CGGCCG C^GGCCG 1633 CGGCCG MslI 504 CACCTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1173 CACTCTGGTG PflFI 1753 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC 1945 GACAATGTC SbfI 456 CCTGCAGG CCTGCA^GG 687 CCTGCAGG SmaI 272 CCCGGG CCC^GGG 1196 CCCGGG Sse8387I 456 CCTGCAGG CCTGCA^GG 687 CCTGCAGG Tth111I 1753 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC 1945 GACAATGTC XcmI 1409 CCATCGAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2114 CCACCAGCCCCCTGG XmaCI 272 CCCGGG C^CCGGG 1196 CCCGGG XmaI 272 CCCGGG C^CCGGG 1196 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI Aor51HI AspHI AvaI AvaII BalI BanI BanII BbeI BbsI BbvCI BbvI BcnI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI Eco47III EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI KasI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV NspI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI StuI StyI TaqI TseI Tsp45I TspRI TthHB8I XhoII