(Circular) (Six-base) MAPSORT of: pFN21AB5757.seq Check: 4294 from: 1 to: 7147 pFN21AB5757 7147 bp With 182 enzymes: SgfI * December 8, 2014 13:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2686 2686 Size: 7147 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4957 278 Size: 53 83 186 936 3421 2468 Fragments arranged by size: 3421 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4490 1937 Size: 2553 4594 Acc65I G'GTAC_C Cuts at: 4468 4468 Size: 7147 AclI AA'CG_TT Cuts at: 1563 5274 5647 1563 Size: 3711 373 3063 Fragments arranged by size: 3711 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2341 2593 5210 6594 6862 1632 Size: 709 252 2617 1384 268 1917 Fragments arranged by size: 2617 1917 1384 709 268 252 AfeI AGC'GCT Cuts at: 2568 3786 4035 2568 Size: 1218 249 5680 Fragments arranged by size: 5680 1218 249 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6950 AflIII A'CryG_T Cuts at: 1203 2706 4338 6046 7023 1203 Size: 1503 1632 1708 977 1327 Fragments arranged by size: 1708 1632 1503 1327 977 AgeI A'CCGG_T Cuts at: 5978 5978 Size: 7147 AhdI GACnn_n'nnGTC Cuts at: 3650 5876 3650 Size: 2226 4921 AleI CACnn'nnGTG Cuts at: 1166 2513 3182 1166 Size: 1347 669 5131 Fragments arranged by size: 5131 1347 669 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3072 3935 1184 Size: 1888 863 4396 Fragments arranged by size: 4396 1888 863 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2000 2032 2000 Size: 32 7115 AlwNI CAG_nnn'CTG Cuts at: 2227 2732 3287 3727 4308 4890 6462 2227 Size: 505 555 440 581 582 1572 2912 Fragments arranged by size: 2912 1572 582 581 555 505 440 ApaI G_GGCC'C Cuts at: 3550 4317 3550 Size: 767 6380 ApaLI G'TGCA_C Cuts at: 5204 6360 5204 Size: 1156 5991 ApoI r'AATT_y Cuts at: 1493 4456 4682 4834 6943 1493 Size: 2963 226 152 2109 1697 Fragments arranged by size: 2963 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5701 160 Size: 872 4669 1606 Fragments arranged by size: 4669 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7147 AvaI C'yCGr_G Cuts at: 1870 1943 2030 2277 2549 2600 2777 3201 3694 Size: 73 87 247 272 51 177 424 493 Cuts at: 3694 3906 4107 4472 1870 Size: 212 201 365 4545 Fragments arranged by size: 4545 493 424 365 272 247 212 201 177 87 73 51 BamHI G'GATC_C Cuts at: 2390 3294 4477 4844 2390 Size: 904 1183 367 4693 Fragments arranged by size: 4693 1183 904 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2106 2113 2507 3166 3404 Size: 325 217 627 319 7 394 659 238 Cuts at: 3404 3515 4347 4468 5923 618 Size: 111 832 121 1455 1842 Fragments arranged by size: 1842 1455 832 659 627 394 325 319 238 217 121 111 7 BanII G_rGCy'C Cuts at: 1391 2277 2607 2625 3120 3550 3572 3588 4256 Size: 886 330 18 495 430 22 16 668 Cuts at: 4256 4317 4466 1391 Size: 61 149 4072 Fragments arranged by size: 4072 886 668 495 430 330 149 61 22 18 16 BbeI G_GCGC'C Cuts at: 2110 2117 3519 2110 Size: 7 1402 5738 Fragments arranged by size: 5738 1402 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2278 2308 2351 3267 928 Size: 971 379 30 43 916 4808 Fragments arranged by size: 4808 971 916 379 43 30 BbvCI CC'TCA_GC Cuts at: 2737 3605 3674 3809 2737 Size: 868 69 135 6075 Fragments arranged by size: 6075 868 135 69 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5338 5372 1903 Size: 34 3401 34 3678 Fragments arranged by size: 3678 3401 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2176 5039 6249 2176 Size: 2863 1210 3074 Fragments arranged by size: 3074 2863 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7147 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2816 3065 3750 Size: 107 122 71 631 605 1144 249 685 Cuts at: 3750 4331 5758 136 Size: 581 1427 1525 Fragments arranged by size: 1525 1427 1144 685 631 605 581 249 122 107 71 BglII A'GATC_T Cuts at: 7142 7142 Size: 7147 BlpI GC'TnA_GC Cuts at: 3069 3233 3314 3566 4037 4250 4564 3069 Size: 164 81 252 471 213 314 5652 Fragments arranged by size: 5652 471 314 252 213 164 81 Bme1580I G_kGCm'C Cuts at: 1790 3169 3550 3803 4317 4350 4417 5208 6364 Size: 1379 381 253 514 33 67 791 1156 Cuts at: 6364 1790 Size: 2573 Fragments arranged by size: 2573 1379 1156 791 514 381 253 67 33 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7147 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2893 4176 4318 Size: 416 53 83 399 24 1476 1283 142 Cuts at: 4318 4878 5836 442 Size: 560 958 1753 Fragments arranged by size: 1753 1476 1283 958 560 416 399 142 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7147 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2738 4062 2738 Size: 1324 5823 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2420 2438 2510 2733 3446 3619 5807 1239 Size: 1181 18 72 223 713 173 2188 2579 Fragments arranged by size: 2579 2188 1181 713 223 173 72 18 Bpu10I CC'TnA_GC Cuts at: 2123 2737 3605 3674 3809 2123 Size: 614 868 69 135 5461 Fragments arranged by size: 5461 868 614 135 69 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3320 5272 6137 6435 3320 Size: 1952 865 298 4032 Fragments arranged by size: 4032 1952 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5810 882 Size: 617 4311 2219 Fragments arranged by size: 4311 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3310 4938 493 Size: 711 2106 1628 2702 Fragments arranged by size: 2702 2106 1628 711 BsaBI GATnn'nnATC Cuts at: 7141 7141 Size: 7147 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2010 2107 2114 2283 Size: 53 83 186 936 477 97 7 169 Cuts at: 2283 2842 3323 3378 3516 3762 3999 4954 5336 Size: 559 481 55 138 246 237 955 382 Cuts at: 5336 5971 275 Size: 635 1451 Fragments arranged by size: 1451 955 936 635 559 481 477 382 246 237 186 169 138 97 83 55 53 7 BsaWI w'CCGG_w Cuts at: 5580 5978 6252 6399 6810 5580 Size: 398 274 147 411 5917 Fragments arranged by size: 5917 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2820 2850 1178 Size: 30 165 30 1417 30 5475 Fragments arranged by size: 5475 1417 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2012 2095 2285 3497 4001 1186 Size: 826 83 190 1212 504 4332 Fragments arranged by size: 4332 1212 826 504 190 83 BseYI C'CCAG_C Cuts at: 2227 3303 3384 3708 4098 6350 6846 2227 Size: 1076 81 324 390 2252 496 2528 Fragments arranged by size: 2528 2252 1076 496 390 324 81 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2517 3050 3122 3499 3650 3908 4076 Size: 573 781 533 72 377 151 258 168 Cuts at: 4076 1163 Size: 4234 Fragments arranged by size: 4234 781 573 533 377 258 168 151 72 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3592 3641 4597 5358 6386 1540 Size: 275 186 1591 49 956 761 1028 2301 Fragments arranged by size: 2301 1591 1028 956 761 275 186 49 BsiHKAI G_wGCw'C Cuts at: 2607 2625 3315 3588 4256 5208 5293 6364 2607 Size: 18 690 273 668 952 85 1071 3390 Fragments arranged by size: 3390 1071 952 690 668 273 85 18 BsmI GAATG_Cn' Cuts at: 4658 4751 4658 Size: 93 7054 BsmBI CGTCTCn'nnnn_ Cuts at: 2189 2546 2709 2189 Size: 357 163 6627 Fragments arranged by size: 6627 357 163 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2277 2607 2625 3120 3169 3315 3550 Size: 399 487 330 18 495 49 146 235 Cuts at: 3550 3572 3588 3803 4256 4317 4350 4417 4466 Size: 22 16 215 453 61 33 67 49 Cuts at: 4466 5208 5293 6364 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 495 487 453 399 330 235 215 146 85 67 61 49 49 33 22 18 16 BspEI T'CCGG_A Cuts at: 6810 6810 Size: 7147 BspHI T'CATG_A Cuts at: 5036 6766 5036 Size: 1730 5417 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2686 2874 3540 4502 844 Size: 1842 188 666 962 3489 Fragments arranged by size: 3489 1842 962 666 188 BsrBI CCG'CTC Cuts at: 1394 1823 3073 5034 1394 Size: 429 1250 1961 3507 Fragments arranged by size: 3507 1961 1250 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5642 5816 65 Size: 1183 4394 174 1396 Fragments arranged by size: 4394 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2448 3401 5791 5978 1700 Size: 110 638 953 2390 187 2869 Fragments arranged by size: 2869 2390 953 638 187 110 BsrGI T'GTAC_A Cuts at: 96 2679 96 Size: 2583 4564 BssHII G'CGCG_C Cuts at: 1925 3629 1925 Size: 1704 5443 BssSI C'ACGA_G Cuts at: 3872 5207 6219 3872 Size: 1335 1012 4800 Fragments arranged by size: 4800 1335 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3477 794 Size: 2683 4464 BstBI TT'CG_AA Cuts at: 5951 5951 Size: 7147 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7147 BstXI CCAn_nnnn'nTGG Cuts at: 2416 3364 4129 2416 Size: 948 765 5434 Fragments arranged by size: 5434 948 765 BstYI r'GATC_y Cuts at: 1972 2390 2634 3294 3420 4477 4844 5229 5246 Size: 418 244 660 126 1057 367 385 17 Cuts at: 5246 6698 6784 6796 7142 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1057 660 418 385 367 346 244 126 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3062 3062 Size: 7147 BtgI C'CryG_G Cuts at: 513 1065 2004 2409 3519 3541 513 Size: 552 939 405 1110 22 4119 Fragments arranged by size: 4119 1110 939 552 405 22 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4120 4290 487 Size: 683 56 2894 170 3344 Fragments arranged by size: 3344 2894 683 170 56 BtsI GCAGTG_nn' Cuts at: 2730 4415 4663 5457 5477 2730 Size: 1685 248 794 20 4400 Fragments arranged by size: 4400 1685 794 248 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7147 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2868 2903 529 Size: 35 2304 35 4773 Fragments arranged by size: 4773 2304 35 35 DraI TTT'AAA Cuts at: 4451 4451 Size: 7147 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6154 809 Size: 507 4838 1802 Fragments arranged by size: 4838 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2450 2479 2889 Size: 54 1425 50 275 18 620 29 410 Cuts at: 2889 3186 3289 3589 3638 3837 4594 5483 7112 Size: 297 103 300 49 199 757 889 1629 Cuts at: 7112 8 Size: 43 Fragments arranged by size: 1629 1425 889 757 620 410 300 297 275 199 103 54 50 49 43 29 18 EagI C'GGCC_G Cuts at: 1537 1812 3589 3638 4594 1537 Size: 275 1777 49 956 4090 Fragments arranged by size: 4090 1777 956 275 49 EarI CTCTTCn'nnn_ Cuts at: 1353 2612 5077 1353 Size: 1259 2465 3423 Fragments arranged by size: 3423 2465 1259 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2717 5734 6108 6254 387 Size: 1064 138 1128 3017 374 146 1280 Fragments arranged by size: 3017 1280 1128 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2605 2623 3586 4254 2605 Size: 18 963 668 5498 Fragments arranged by size: 5498 963 668 18 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2341 2420 2438 2510 2593 2733 3446 Size: 393 709 79 18 72 83 140 713 Cuts at: 3446 3619 5210 5807 6594 6862 1239 Size: 173 1591 597 787 268 1524 Fragments arranged by size: 1591 1524 787 713 709 597 393 268 173 140 83 79 72 18 EcoNI CCTnn'n_nnAGG Cuts at: 2465 2588 3158 3368 3608 2465 Size: 123 570 210 240 6004 Fragments arranged by size: 6004 570 240 210 123 EcoO109I rG'GnC_Cy Cuts at: 2139 2255 2404 2863 2983 3040 3205 3529 3546 Size: 116 149 459 120 57 165 324 17 Cuts at: 3546 3547 3652 4313 4314 2139 Size: 1 105 661 1 4972 Fragments arranged by size: 4972 661 459 324 165 149 120 116 105 57 17 1 1 EcoRI G'AATT_C Cuts at: 4456 4834 4456 Size: 378 6769 FspI TGC'GCA Cuts at: 5653 5653 Size: 7147 HaeII r_GCGC'y Cuts at: 1678 2110 2117 2570 3519 3788 3846 4037 6294 Size: 432 7 453 949 269 58 191 2257 Cuts at: 6294 1678 Size: 2531 Fragments arranged by size: 2531 2257 949 453 432 269 191 58 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3345 3365 3377 3397 3636 Size: 32 135 32 1773 20 12 20 239 Cuts at: 3636 3668 3939 3971 5788 5820 5862 5894 1373 Size: 32 271 32 1817 32 42 32 2626 Fragments arranged by size: 2626 1817 1773 271 239 135 42 32 32 32 32 32 32 20 20 12 HincII GTy'rAC Cuts at: 669 1657 1938 3616 4491 4737 669 Size: 988 281 1678 875 246 3079 Fragments arranged by size: 3079 1678 988 875 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7147 HpaI GTT'AAC Cuts at: 4737 4737 Size: 7147 KasI G'GCGC_C Cuts at: 2106 2113 3515 2106 Size: 7 1402 5738 Fragments arranged by size: 5738 1402 7 KpnI G_GTAC'C Cuts at: 4472 4472 Size: 7147 MfeI C'AATT_G Cuts at: 4746 4746 Size: 7147 MluI A'CGCG_T Cuts at: 7023 7023 Size: 7147 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2193 4341 6261 6445 1305 Size: 888 2148 1920 184 2007 Fragments arranged by size: 2148 2007 1920 888 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2891 3188 3839 10 Size: 54 1425 343 1059 297 651 3318 Fragments arranged by size: 3318 1425 1059 651 343 297 54 MslI CAynn'nnrTG Cuts at: 518 1166 2513 3182 5105 5464 5623 518 Size: 648 1347 669 1923 359 159 2042 Fragments arranged by size: 2042 1923 1347 669 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2227 2826 3046 3223 3565 3747 4018 4563 Size: 621 599 220 177 342 182 271 545 Cuts at: 4563 5240 6388 6633 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 621 599 545 342 271 245 220 182 177 NarI GG'CG_CC Cuts at: 2107 2114 3516 2107 Size: 7 1402 5738 Fragments arranged by size: 5738 1402 7 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5656 Fragments arranged by size: 5656 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7147 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7147 NotI GC'GGCC_GC Cuts at: 3638 4594 3638 Size: 956 6191 NspI r_CATG'y Cuts at: 1132 1229 2710 3101 4342 4505 6050 1132 Size: 97 1481 391 1241 163 1545 2229 Fragments arranged by size: 2229 1545 1481 1241 391 163 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7147 PciI A'CATG_T Cuts at: 2706 4338 6046 2706 Size: 1632 1708 3807 Fragments arranged by size: 3807 1708 1632 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7147 PfoI T'CCnGG_A Cuts at: 2071 2904 3129 3423 3687 2071 Size: 833 225 294 264 5531 Fragments arranged by size: 5531 833 294 264 225 PmeI GTTT'AAAC Cuts at: 4451 4451 Size: 7147 PmlI CAC'GTG Cuts at: 3310 3310 Size: 7147 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5216 5248 6729 6761 5216 Size: 32 1481 32 5602 Fragments arranged by size: 5602 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2255 2404 2863 3205 3529 3652 2255 Size: 149 459 342 324 123 5750 Fragments arranged by size: 5750 459 342 324 149 123 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7147 PsiI TTA'TAA Cuts at: 4717 4717 Size: 7147 PspOMI G'GGCC_C Cuts at: 3546 4313 3546 Size: 767 6380 PstI C_TGCA'G Cuts at: 830 2466 2589 2697 3051 3228 4171 4499 830 Size: 1636 123 108 354 177 943 328 3478 Fragments arranged by size: 3478 1636 943 354 328 177 123 108 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7147 PvuII CAG'CTG Cuts at: 2227 3223 3565 4018 2227 Size: 996 342 453 5356 Fragments arranged by size: 5356 996 453 342 SacI G_AGCT'C Cuts at: 2607 2625 3588 4256 2607 Size: 18 963 668 5498 Fragments arranged by size: 5498 963 668 18 SalI G'TCGA_C Cuts at: 1936 4489 1936 Size: 2553 4594 SanDI GG'GwC_CC Cuts at: 2255 3205 2255 Size: 950 6197 SapI GCTCTTCn'nnn_ Cuts at: 2612 2612 Size: 7147 SbfI CC_TGCA'GG Cuts at: 2466 2697 4499 2466 Size: 231 1802 5114 Fragments arranged by size: 5114 1802 231 ScaI AGT'ACT Cuts at: 5395 7107 5395 Size: 1712 5435 SexAI A'CCwGG_T Cuts at: 3648 3648 Size: 7147 SfcI C'TryA_G Cuts at: 826 1045 2242 2462 2585 2693 3047 3224 4167 Size: 219 1197 220 123 108 354 177 943 Cuts at: 4167 4495 5630 6311 6502 826 Size: 328 1135 681 191 1471 Fragments arranged by size: 1471 1197 1135 943 681 354 328 220 219 191 177 123 108 SfiI GGCCn_nnn'nGGCC Cuts at: 2816 2816 Size: 7147 SfoI GGC'GCC Cuts at: 2108 2115 3517 2108 Size: 7 1402 5738 Fragments arranged by size: 5738 1402 7 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7147 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7147 SmaI CCC'GGG Cuts at: 1872 2279 3203 4474 1872 Size: 407 924 1271 4545 Fragments arranged by size: 4545 1271 924 407 SmlI C'TyrA_G Cuts at: 820 1017 1943 3335 5251 6152 6414 820 Size: 197 926 1392 1916 901 262 1553 Fragments arranged by size: 1916 1553 1392 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4938 493 Size: 4445 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7147 SphI G_CATG'C Cuts at: 1132 4505 1132 Size: 3373 3774 SspI AAT'ATT Cuts at: 5 52 5071 5 Size: 47 5019 2081 Fragments arranged by size: 5019 2081 47 StuI AGG'CCT Cuts at: 1517 3504 3663 3807 1517 Size: 1987 159 144 4857 Fragments arranged by size: 4857 1987 159 144 StyI C'CwwG_G Cuts at: 513 1065 2004 3084 3243 3493 4586 513 Size: 552 939 1080 159 250 1093 3074 Fragments arranged by size: 3074 1093 1080 939 552 250 159 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2875 5174 5191 5344 5529 5974 325 Size: 1255 1295 2299 17 153 185 445 1498 Fragments arranged by size: 2299 1498 1295 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2679 5393 7105 Size: 274 80 33 51 1443 702 2714 1712 Cuts at: 7105 96 Size: 138 Fragments arranged by size: 2714 1712 1443 702 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2247 2279 2820 2852 7107 7139 2247 Size: 32 541 32 4255 32 2255 Fragments arranged by size: 4255 2255 541 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3761 3953 1316 Size: 2445 192 4510 Fragments arranged by size: 4510 2445 192 XbaI T'CTAG_A Cuts at: 4483 4483 Size: 7147 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3421 4126 3421 Size: 705 6442 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7147 XmaI C'CCGG_G Cuts at: 1870 2277 3201 4472 1870 Size: 407 924 1271 4545 Fragments arranged by size: 4545 1271 924 407 XmnI GAAnn'nnTTC Cuts at: 2315 5276 2315 Size: 2961 4186 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4955 276 Size: 53 83 186 936 3421 2468 Fragments arranged by size: 3421 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII SrfI SwaI