(Linear) (Six-base) MAPSORT of: KIBB5757.seq Check: 5983 from: 1 to: 2442 KIBB5757.orf With 182 enzymes: SgfI * December 5, 2014 12:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 681 2442 Size: 681 1761 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 336 588 2442 Size: 336 252 1854 Fragments arranged by size: 1854 336 252 AfeI AGC'GCT Cuts at: 0 563 1781 2030 2442 Size: 563 1218 249 412 Fragments arranged by size: 1218 563 412 249 AflIII A'CryG_T Cuts at: 0 701 2333 2442 Size: 701 1632 109 Fragments arranged by size: 1632 701 109 AhdI GACnn_n'nnGTC Cuts at: 0 1645 2442 Size: 1645 797 AleI CACnn'nnGTG Cuts at: 0 508 1177 2442 Size: 508 669 1265 Fragments arranged by size: 1265 669 508 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1067 1930 2442 Size: 1067 863 512 Fragments arranged by size: 1067 863 512 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 27 2442 Size: 27 2415 AlwNI CAG_nnn'CTG Cuts at: 0 222 727 1282 1722 2303 2442 Size: 222 505 555 440 581 139 Fragments arranged by size: 581 555 505 440 222 139 ApaI G_GGCC'C Cuts at: 0 1545 2312 2442 Size: 1545 767 130 Fragments arranged by size: 1545 767 130 AvaI C'yCGr_G Cuts at: 0 25 272 544 595 772 1196 1689 1901 Size: 25 247 272 51 177 424 493 212 Cuts at: 1901 2102 2442 Size: 201 340 Fragments arranged by size: 493 424 340 272 247 212 201 177 51 25 BamHI G'GATC_C Cuts at: 0 385 1289 2442 Size: 385 904 1153 Fragments arranged by size: 1153 904 385 BanI G'GyrC_C Cuts at: 0 101 108 502 1161 1399 1510 2342 2442 Size: 101 7 394 659 238 111 832 100 Fragments arranged by size: 832 659 394 238 111 101 100 7 BanII G_rGCy'C Cuts at: 0 272 602 620 1115 1545 1567 1583 2251 Size: 272 330 18 495 430 22 16 668 Cuts at: 2251 2312 2442 Size: 61 130 Fragments arranged by size: 668 495 430 330 272 130 61 22 18 16 BbeI G_GCGC'C Cuts at: 0 105 112 1514 2442 Size: 105 7 1402 928 Fragments arranged by size: 1402 928 105 7 BbsI GAAGACnn'nnnn_ Cuts at: 0 273 303 346 1262 2442 Size: 273 30 43 916 1180 Fragments arranged by size: 1180 916 273 43 30 BbvCI CC'TCA_GC Cuts at: 0 732 1600 1669 1804 2442 Size: 732 868 69 135 638 Fragments arranged by size: 868 732 638 135 69 BciVI GTATCCnnnnn_n' Cuts at: 0 171 2442 Size: 171 2271 BglI GCCn_nnn'nGGC Cuts at: 0 811 1060 1745 2326 2442 Size: 811 249 685 581 116 Fragments arranged by size: 811 685 581 249 116 BlpI GC'TnA_GC Cuts at: 0 1064 1228 1309 1561 2032 2245 2442 Size: 1064 164 81 252 471 213 197 Fragments arranged by size: 1064 471 252 213 197 164 81 Bme1580I G_kGCm'C Cuts at: 0 1164 1545 1798 2312 2345 2412 2442 Size: 1164 381 253 514 33 67 30 Fragments arranged by size: 1164 514 381 253 67 33 30 BmrI ACTGGGnnnn_n' Cuts at: 0 888 2171 2313 2442 Size: 888 1283 142 129 Fragments arranged by size: 1283 888 142 129 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 733 2057 2442 Size: 733 1324 385 Fragments arranged by size: 1324 733 385 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 415 433 505 728 1441 1614 2442 Size: 415 18 72 223 713 173 828 Fragments arranged by size: 828 713 415 223 173 72 18 Bpu10I CC'TnA_GC Cuts at: 0 118 732 1600 1669 1804 2442 Size: 118 614 868 69 135 638 Fragments arranged by size: 868 638 614 135 118 69 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1315 2442 Size: 1315 1127 BsaAI yAC'GTr Cuts at: 0 1305 2442 Size: 1305 1137 BsaHI Gr'CG_yC Cuts at: 0 5 102 109 278 837 1318 1373 1511 Size: 5 97 7 169 559 481 55 138 Cuts at: 1511 1757 1994 2442 Size: 246 237 448 Fragments arranged by size: 559 481 448 246 237 169 138 97 55 7 5 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 815 845 2442 Size: 815 30 1597 Fragments arranged by size: 1597 815 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 7 90 280 1492 1996 2442 Size: 7 83 190 1212 504 446 Fragments arranged by size: 1212 504 446 190 83 7 BseYI C'CCAG_C Cuts at: 0 222 1298 1379 1703 2093 2442 Size: 222 1076 81 324 390 349 Fragments arranged by size: 1076 390 349 324 222 81 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 512 1045 1117 1494 1645 1903 2071 2442 Size: 512 533 72 377 151 258 168 371 Fragments arranged by size: 533 512 377 371 258 168 151 72 BsiEI CG_ry'CG Cuts at: 0 1587 1636 2442 Size: 1587 49 806 Fragments arranged by size: 1587 806 49 BsiHKAI G_wGCw'C Cuts at: 0 602 620 1310 1583 2251 2442 Size: 602 18 690 273 668 191 Fragments arranged by size: 690 668 602 273 191 18 BsmBI CGTCTCn'nnnn_ Cuts at: 0 184 541 704 2442 Size: 184 357 163 1738 Fragments arranged by size: 1738 357 184 163 Bsp1286I G_dGCh'C Cuts at: 0 272 602 620 1115 1164 1310 1545 1567 Size: 272 330 18 495 49 146 235 22 Cuts at: 1567 1583 1798 2251 2312 2345 2412 2442 Size: 16 215 453 61 33 67 30 Fragments arranged by size: 495 453 330 272 235 215 146 67 61 49 33 30 22 18 16 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 681 869 1535 2442 Size: 681 188 666 907 Fragments arranged by size: 907 681 666 188 BsrBI CCG'CTC Cuts at: 0 1068 2442 Size: 1068 1374 BsrFI r'CCGG_y Cuts at: 0 443 1396 2442 Size: 443 953 1046 Fragments arranged by size: 1046 953 443 BsrGI T'GTAC_A Cuts at: 0 674 2442 Size: 674 1768 BssHII G'CGCG_C Cuts at: 0 1624 2442 Size: 1624 818 BssSI C'ACGA_G Cuts at: 0 1867 2442 Size: 1867 575 BstAPI GCAn_nnn'nTGC Cuts at: 0 1472 2442 Size: 1472 970 BstXI CCAn_nnnn'nTGG Cuts at: 0 411 1359 2124 2442 Size: 411 948 765 318 Fragments arranged by size: 948 765 411 318 BstYI r'GATC_y Cuts at: 0 385 629 1289 1415 2442 Size: 385 244 660 126 1027 Fragments arranged by size: 1027 660 385 244 126 Bsu36I CC'TnA_GG Cuts at: 0 1057 2442 Size: 1057 1385 BtgI C'CryG_G Cuts at: 0 404 1514 1536 2442 Size: 404 1110 22 906 Fragments arranged by size: 1110 906 404 22 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2115 2285 2442 Size: 2115 170 157 Fragments arranged by size: 2115 170 157 BtsI GCAGTG_nn' Cuts at: 0 725 2410 2442 Size: 725 1685 32 Fragments arranged by size: 1685 725 32 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 863 898 2442 Size: 863 35 1544 Fragments arranged by size: 1544 863 35 EaeI y'GGCC_r Cuts at: 0 445 474 884 1181 1284 1584 1633 1832 Size: 445 29 410 297 103 300 49 199 Cuts at: 1832 2442 Size: 610 Fragments arranged by size: 610 445 410 300 297 199 103 49 29 EagI C'GGCC_G Cuts at: 0 1584 1633 2442 Size: 1584 49 809 Fragments arranged by size: 1584 809 49 EarI CTCTTCn'nnn_ Cuts at: 0 607 2442 Size: 607 1835 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 712 2442 Size: 712 1730 EcoICRI GAG'CTC Cuts at: 0 600 618 1581 2249 2442 Size: 600 18 963 668 193 Fragments arranged by size: 963 668 600 193 18 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 336 415 433 505 588 728 1441 1614 Size: 336 79 18 72 83 140 713 173 Cuts at: 1614 2442 Size: 828 Fragments arranged by size: 828 713 336 173 140 83 79 72 18 EcoNI CCTnn'n_nnAGG Cuts at: 0 460 583 1153 1363 1603 2442 Size: 460 123 570 210 240 839 Fragments arranged by size: 839 570 460 240 210 123 EcoO109I rG'GnC_Cy Cuts at: 0 134 250 399 858 978 1035 1200 1524 Size: 134 116 149 459 120 57 165 324 Cuts at: 1524 1541 1542 1647 2308 2309 2442 Size: 17 1 105 661 1 133 Fragments arranged by size: 661 459 324 165 149 134 133 120 116 105 57 17 1 1 HaeII r_GCGC'y Cuts at: 0 105 112 565 1514 1783 1841 2032 2442 Size: 105 7 453 949 269 58 191 410 Fragments arranged by size: 949 453 410 269 191 105 58 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1340 1360 1372 1392 1631 1663 1934 1966 Size: 1340 20 12 20 239 32 271 32 Cuts at: 1966 2442 Size: 476 Fragments arranged by size: 1340 476 271 239 32 32 20 20 12 HincII GTy'rAC Cuts at: 0 1611 2442 Size: 1611 831 KasI G'GCGC_C Cuts at: 0 101 108 1510 2442 Size: 101 7 1402 932 Fragments arranged by size: 1402 932 101 7 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 188 2336 2442 Size: 188 2148 106 Fragments arranged by size: 2148 188 106 MscI TGG'CCA Cuts at: 0 886 1183 1834 2442 Size: 886 297 651 608 Fragments arranged by size: 886 651 608 297 MslI CAynn'nnrTG Cuts at: 0 508 1177 2442 Size: 508 669 1265 Fragments arranged by size: 1265 669 508 MspA1I CmG'CkG Cuts at: 0 222 821 1041 1218 1560 1742 2013 2442 Size: 222 599 220 177 342 182 271 429 Fragments arranged by size: 599 429 342 271 222 220 182 177 NarI GG'CG_CC Cuts at: 0 102 109 1511 2442 Size: 102 7 1402 931 Fragments arranged by size: 1402 931 102 7 NotI GC'GGCC_GC Cuts at: 0 1633 2442 Size: 1633 809 NspI r_CATG'y Cuts at: 0 705 1096 2337 2442 Size: 705 391 1241 105 Fragments arranged by size: 1241 705 391 105 PciI A'CATG_T Cuts at: 0 701 2333 2442 Size: 701 1632 109 Fragments arranged by size: 1632 701 109 PfoI T'CCnGG_A Cuts at: 0 66 899 1124 1418 1682 2442 Size: 66 833 225 294 264 760 Fragments arranged by size: 833 760 294 264 225 66 PmlI CAC'GTG Cuts at: 0 1305 2442 Size: 1305 1137 PpuMI rG'GwC_Cy Cuts at: 0 250 399 858 1200 1524 1647 2442 Size: 250 149 459 342 324 123 795 Fragments arranged by size: 795 459 342 324 250 149 123 PspOMI G'GGCC_C Cuts at: 0 1541 2308 2442 Size: 1541 767 134 Fragments arranged by size: 1541 767 134 PstI C_TGCA'G Cuts at: 0 461 584 692 1046 1223 2166 2442 Size: 461 123 108 354 177 943 276 Fragments arranged by size: 943 461 354 276 177 123 108 PvuII CAG'CTG Cuts at: 0 222 1218 1560 2013 2442 Size: 222 996 342 453 429 Fragments arranged by size: 996 453 429 342 222 SacI G_AGCT'C Cuts at: 0 602 620 1583 2251 2442 Size: 602 18 963 668 191 Fragments arranged by size: 963 668 602 191 18 SanDI GG'GwC_CC Cuts at: 0 250 1200 2442 Size: 250 950 1242 Fragments arranged by size: 1242 950 250 SapI GCTCTTCn'nnn_ Cuts at: 0 607 2442 Size: 607 1835 SbfI CC_TGCA'GG Cuts at: 0 461 692 2442 Size: 461 231 1750 Fragments arranged by size: 1750 461 231 SexAI A'CCwGG_T Cuts at: 0 1643 2442 Size: 1643 799 SfcI C'TryA_G Cuts at: 0 237 457 580 688 1042 1219 2162 2442 Size: 237 220 123 108 354 177 943 280 Fragments arranged by size: 943 354 280 237 220 177 123 108 SfiI GGCCn_nnn'nGGCC Cuts at: 0 811 2442 Size: 811 1631 SfoI GGC'GCC Cuts at: 0 103 110 1512 2442 Size: 103 7 1402 930 Fragments arranged by size: 1402 930 103 7 SmaI CCC'GGG Cuts at: 0 274 1198 2442 Size: 274 924 1244 Fragments arranged by size: 1244 924 274 SmlI C'TyrA_G Cuts at: 0 1330 2442 Size: 1330 1112 StuI AGG'CCT Cuts at: 0 1499 1658 1802 2442 Size: 1499 159 144 640 Fragments arranged by size: 1499 640 159 144 StyI C'CwwG_G Cuts at: 0 1079 1238 1488 2442 Size: 1079 159 250 954 Fragments arranged by size: 1079 954 250 159 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 870 2442 Size: 870 1572 TatI w'GTAC_w Cuts at: 0 674 2442 Size: 674 1768 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 242 274 815 847 2442 Size: 242 32 541 32 1595 Fragments arranged by size: 1595 541 242 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1756 1948 2442 Size: 1756 192 494 Fragments arranged by size: 1756 494 192 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1416 2121 2442 Size: 1416 705 321 Fragments arranged by size: 1416 705 321 XmaI C'CCGG_G Cuts at: 0 272 1196 2442 Size: 272 924 1246 Fragments arranged by size: 1246 924 272 XmnI GAAnn'nnTTC Cuts at: 0 310 2442 Size: 310 2132 Enzymes that do cut: AarI AcuI AfeI AflIII AhdI AleI AlfI AloI AlwNI ApaI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NarI NotI NspI PciI PfoI PmlI PpuMI PspOMI PstI PvuII SacI SanDI SapI SbfI SexAI SfcI SfiI SfoI SmaI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AgeI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BcgI BclI BfrBI BglII BmgBI BmtI BsaI BsaBI BsaWI BsiWI BsmI BspEI BspHI BsrDI BstBI BstEII BstZ17I ClaI DraI DraIII DrdI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI NaeI NcoI NdeI NgoMIV NheI NruI NsiI PacI PasI PflMI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI ScaI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI ZraI