################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## AatII AccI AccII AccIII AclI AflII AflIII AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI AvrII BamHI BbeI BbsI BcgI BclI BfrI BlnI BlpI Bpu1102I BpuAI BsaWI BseAI BseRI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI EarI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HaeII HindIII HinfI HpaI KasI Ksp632I KspI MfeI MluI MroI MseI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PleI PmeI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 66 enzymes) # ################################### Acc65I 620 GGTACC G^GTACC AcyI 258 GACGCC G[GA]^CG[CT]C AhdI 705 GACTGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 38 CCTCAGG CC^T[ACGT]AGG ApaI 831 GGGCCC GGGCC^C Asp700I 483 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 620 GGTACC G^GTACC AspEI 705 GACTGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 402 GAGCAC G[AT]GC[AT]^C AspI 688 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 347 TGCGCA TGC^GCA BalI 104 TGGCCA TGG^CCA BbrPI 222 CACGTG CAC^GTG BbvCI 420 CCTCAGC CCTCAGC(-5/-2) BbvI 468 GCTGC GCAGC(8/12) BciVI 566 GTATCC GTATCC(6/5) BfaI 679 CTAG C^TAG BglII 807 AGATCT A^GATCT BsaAI 222 CACGTG [CT]AC^GT[GA] BsaBI 561 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 258 GACGCC G[GA]^CG[CT]C BsaI 628 GAGACC GGTCTC(1/5) BsiHKAI 402 GAGCAC G[AT]GC[AT]^C BsmFI 91 GTCCC GGGAC(10/14) BspMI 440 ACCTGC ACCTGC(4/8) BssSI 868 CACGAG CACGAG(-5/-1) Bsu36I 38 CCTCAGG CC^T[ACGT]AGG CfoI 348 GCGC GCG^C DsaI 459 CCATGG C^C[GA][CT]GG Eam1105I 705 GACTGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 38 CCTCAGG CC^T[ACGT]AGG EcoT14I 459 CCATGG C^C[AT][AT]GG FauI 498 GCGGG CCCGC(4/6) FspI 347 TGCGCA TGC^GCA HgaI 258 GACGC GACGC(5/10) HhaI 348 GCGC GCG^C Hin1I 258 GACGCC G[GA]^CG[CT]C HinP1I 348 GCGC G^CGC HincII 733 GTCAAC GT[CT]^[GA]AC HindII 733 GTCAAC GT[CT]^[GA]AC HphI 702 GGTGA GGTGA(8/7) KpnI 620 GGTACC GGTAC^C MaeI 679 CTAG C^TAG MaeIII 703 GTGAC ^GT[ACGT]AC MamI 561 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 104 TGGCCA TGG^CCA MscI 104 TGGCCA TGG^CCA MslI 300 CATCATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 656 CAGCGG C[AC]G^C[GT]G NcoI 459 CCATGG C^CATGG NlaIII 460 CATG CATG^ PflFI 688 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 222 CACGTG CAC^GTG PmlI 222 CACGTG CAC^GTG PstI 469 CTGCAG CTGCA^G SfcI 469 CTGCAG C^T[GA][CT]AG SfiI 156 GGCCCAGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 544 CTCAAG C^T[CT][GA]AG StyI 459 CCATGG C^C[AT][AT]GG TaqI 77 TCGA T^CGA TseI 468 GCTGC G^C[AT]GC Tsp45I 703 GTGAC ^GT[GC]AC TspRI 141 CACTG CA[GC]TG[ACGT][ACGT]^ Tth111I 688 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 77 TCGA T^CGA XmnI 483 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AcsI 61 AAATTT [GA]^AATT[CT] 364 GAATTT ApoI 61 AAATTT [GA]^AATT[CT] 364 GAATTT BanI 27 GGTGCC G^G[CT][GA]CC 620 GGTACC BglI 36 GCCCTCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 157 GCCCAGGCGGC BsmBI 693 CGTCTC CGTCTC(1/5) 256 GAGACG EaeI 104 TGGCCA [CT]^GGCC[GA] 572 TGGCCG EcoNI 75 CCTCGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 202 CCTCTGGAAGG PpuMI 89 AGGTCCC [GA]G^G[AT]CC[CT] 500 GGGTCCT SfaNI 299 GCATC GCATC(5/9) 796 GCATC SmaI 280 CCCGGG CCC^GGG 698 CCCGGG StuI 638 AGGCCT AGG^CCT 825 AGGCCT XmaCI 280 CCCGGG C^CCGGG 698 CCCGGG XmaI 280 CCCGGG C^CCGGG 698 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AvaI AvaII BanII BcnI BmyI BpmI BsaJI BsgI BsiYI BslI BsmAI BsoBI Bsp1286I BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HpaII ItaI MaeII MboI MboII MflI MnlI MspI MvaI MwoI NciI NdeII NlaIV RsaI Sau3AI Sau96I ScrFI Tsp509I XhoII