(Circular) (Six-base) MAPSORT of: pFN21AE2306.seq Check: 2342 from: 1 to: 5596 pFN21AE2306 5596 bp With 182 enzymes: SgfI * November 16, 2010 14:16 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3406 278 Size: 53 83 186 936 1870 2468 Fragments arranged by size: 2468 1870 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2939 1937 Size: 1002 4594 Acc65I G'GTAC_C Cuts at: 2625 2917 2625 Size: 292 5304 AclI AA'CG_TT Cuts at: 1563 3723 4096 1563 Size: 2160 373 3063 Fragments arranged by size: 3063 2160 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2363 3659 5043 5311 1632 Size: 731 1296 1384 268 1917 Fragments arranged by size: 1917 1384 1296 731 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5399 AflIII A'CryG_T Cuts at: 1203 4495 5472 1203 Size: 3292 977 1327 Fragments arranged by size: 3292 1327 977 AgeI A'CCGG_T Cuts at: 4427 4427 Size: 5596 AhdI GACnn_n'nnGTC Cuts at: 2715 4325 2715 Size: 1610 3986 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5596 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5596 AlwNI CAG_nnn'CTG Cuts at: 2209 2278 2482 3339 4911 2209 Size: 69 204 857 1572 2894 Fragments arranged by size: 2894 1572 857 204 69 ApaI G_GGCC'C Cuts at: 2840 2840 Size: 5596 ApaLI G'TGCA_C Cuts at: 3653 4809 3653 Size: 1156 4440 ApoI r'AATT_y Cuts at: 1493 2066 2369 2905 3131 3283 5392 1493 Size: 573 303 536 226 152 2109 1697 Fragments arranged by size: 2109 1697 573 536 303 226 152 AseI AT'TA_AT Cuts at: 160 1032 4150 160 Size: 872 3118 1606 Fragments arranged by size: 3118 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5596 AvaI C'yCGr_G Cuts at: 1870 1943 2285 2609 2703 2921 1870 Size: 73 342 324 94 218 4545 Fragments arranged by size: 4545 342 324 218 94 73 BamHI G'GATC_C Cuts at: 2926 3293 2926 Size: 367 5229 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2032 2625 2917 4372 618 Size: 325 217 627 245 593 292 1455 1842 Fragments arranged by size: 1842 1455 627 593 325 292 245 217 BanII G_rGCy'C Cuts at: 1391 2250 2318 2609 2840 2880 2915 1391 Size: 859 68 291 231 40 35 4072 Fragments arranged by size: 4072 859 291 231 68 40 35 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4625 BbvCI CC'TCA_GC Cuts at: 2426 2426 Size: 5596 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3787 3821 1903 Size: 34 1850 34 3678 Fragments arranged by size: 3678 1850 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2582 3488 4698 2582 Size: 906 1210 3480 Fragments arranged by size: 3480 1210 906 BclI T'GATC_A Cuts at: 1548 1548 Size: 5596 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2047 2168 4207 Size: 107 122 71 631 605 375 121 2039 Cuts at: 4207 136 Size: 1525 Fragments arranged by size: 2039 1525 631 605 375 122 121 107 71 BglII A'GATC_T Cuts at: 2812 5591 2812 Size: 2779 2817 BlpI GC'TnA_GC Cuts at: 3013 3013 Size: 5596 Bme1580I G_kGCm'C Cuts at: 1790 2037 2234 2364 2419 2840 3657 4813 1790 Size: 247 197 130 55 421 817 1156 2573 Fragments arranged by size: 2573 1156 817 421 247 197 130 55 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5596 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3327 4285 442 Size: 416 53 83 399 24 1910 958 1753 Fragments arranged by size: 1910 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5596 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2124 2623 2651 4256 1239 Size: 885 499 28 1605 2579 Fragments arranged by size: 2579 1605 885 499 28 Bpu10I CC'TnA_GC Cuts at: 2426 2426 Size: 5596 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2534 3721 4586 4884 2534 Size: 1187 865 298 3246 Fragments arranged by size: 3246 1187 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2627 4259 882 Size: 617 1128 1632 2219 Fragments arranged by size: 2219 1632 1128 617 BsaAI yAC'GTr Cuts at: 493 1204 2229 3387 493 Size: 711 1025 1158 2702 Fragments arranged by size: 2702 1158 1025 711 BsaBI GATnn'nnATC Cuts at: 2570 5590 2570 Size: 3020 2576 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2264 3403 3785 4420 Size: 53 83 186 936 731 1139 382 635 Cuts at: 4420 275 Size: 1451 Fragments arranged by size: 1451 1139 936 731 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4029 4427 4701 4848 5259 4029 Size: 398 274 147 411 4366 Fragments arranged by size: 4366 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2684 2714 1178 Size: 30 165 30 1281 30 4060 Fragments arranged by size: 4060 1281 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5596 BseYI C'CCAG_C Cuts at: 2037 2219 2398 2538 2618 4799 5295 2037 Size: 182 179 140 80 2181 496 2338 Fragments arranged by size: 2338 2181 496 182 179 140 80 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2432 2569 2855 1163 Size: 573 696 137 286 3904 Fragments arranged by size: 3904 696 573 286 137 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3046 3807 4835 1540 Size: 275 186 1045 761 1028 2301 Fragments arranged by size: 2301 1045 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2411 3657 3742 4813 2411 Size: 1246 85 1071 3194 Fragments arranged by size: 3194 1246 1071 85 BsmI GAATG_Cn' Cuts at: 3107 3200 3107 Size: 93 5503 BsmBI CGTCTCn'nnnn_ Cuts at: 2255 2704 2255 Size: 449 5147 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2037 2234 2250 2318 2364 2411 2419 Size: 399 247 197 16 68 46 47 8 Cuts at: 2419 2609 2840 2880 2915 3657 3742 4813 1391 Size: 190 231 40 35 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 247 231 197 190 85 68 47 46 40 35 16 8 BspEI T'CCGG_A Cuts at: 5259 5259 Size: 5596 BspHI T'CATG_A Cuts at: 3485 5215 3485 Size: 1730 3866 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2454 2951 844 Size: 1610 497 3489 Fragments arranged by size: 3489 1610 497 BsrBI CCG'CTC Cuts at: 1394 1823 3483 1394 Size: 429 1660 3507 Fragments arranged by size: 3507 1660 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4091 4265 65 Size: 1183 2843 174 1396 Fragments arranged by size: 2843 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4240 4427 1700 Size: 110 2430 187 2869 Fragments arranged by size: 2869 2430 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5596 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5596 BssSI C'ACGA_G Cuts at: 2873 3656 4668 2873 Size: 783 1012 3801 Fragments arranged by size: 3801 1012 783 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5596 BstBI TT'CG_AA Cuts at: 4400 4400 Size: 5596 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5596 BstYI r'GATC_y Cuts at: 1972 2533 2777 2812 2926 3293 3678 3695 5147 Size: 561 244 35 114 367 385 17 1452 Cuts at: 5147 5233 5245 5591 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 561 385 367 346 244 114 86 35 17 12 Bsu36I CC'TnA_GG Cuts at: 2044 2044 Size: 5596 BtgI C'CryG_G Cuts at: 513 1065 2004 2464 513 Size: 552 939 460 3645 Fragments arranged by size: 3645 939 552 460 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2779 487 Size: 683 56 1553 3304 Fragments arranged by size: 3304 1553 683 56 BtsI GCAGTG_nn' Cuts at: 3112 3906 3926 3112 Size: 794 20 4782 Fragments arranged by size: 4782 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5596 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5561 DraI TTT'AAA Cuts at: 2900 2900 Size: 5596 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4603 809 Size: 507 3287 1802 Fragments arranged by size: 3287 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2109 2577 3043 Size: 54 1425 50 275 18 279 468 466 Cuts at: 3043 3932 5561 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 468 466 279 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3043 1537 Size: 275 1231 4090 Fragments arranged by size: 4090 1231 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3526 1353 Size: 2173 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4183 4557 4703 387 Size: 1064 138 2594 374 146 1280 Fragments arranged by size: 2594 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2124 2363 2623 2651 3659 4256 5043 Size: 393 492 239 260 28 1008 597 787 Cuts at: 5043 5311 1239 Size: 268 1524 Fragments arranged by size: 1524 1008 787 597 492 393 268 260 239 28 EcoNI CCTnn'n_nnAGG Cuts at: 2084 2211 2084 Size: 127 5469 EcoO109I rG'GnC_Cy Cuts at: 2095 2275 2506 2545 2837 2095 Size: 180 231 39 292 4854 Fragments arranged by size: 4854 292 231 180 39 EcoRI G'AATT_C Cuts at: 2905 3283 2905 Size: 378 5218 FspI TGC'GCA Cuts at: 2354 4102 2354 Size: 1748 3848 HaeII r_GCGC'y Cuts at: 1678 4743 1678 Size: 3065 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2317 2349 2684 2701 2716 Size: 32 135 32 745 32 335 17 15 Cuts at: 2716 2733 4237 4269 4311 4343 1373 Size: 17 1504 32 42 32 2626 Fragments arranged by size: 2626 1504 745 335 135 42 32 32 32 32 32 17 17 15 HincII GTy'rAC Cuts at: 669 1657 1938 2740 2940 3186 669 Size: 988 281 802 200 246 3079 Fragments arranged by size: 3079 988 802 281 246 200 HindIII A'AGCT_T Cuts at: 748 748 Size: 5596 HpaI GTT'AAC Cuts at: 3186 3186 Size: 5596 KpnI G_GTAC'C Cuts at: 2629 2921 2629 Size: 292 5304 MfeI C'AATT_G Cuts at: 3195 3195 Size: 5596 MluI A'CGCG_T Cuts at: 5472 5472 Size: 5596 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2322 4710 4894 1305 Size: 1017 2388 184 2007 Fragments arranged by size: 2388 2007 1017 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2111 10 Size: 54 1425 343 279 3495 Fragments arranged by size: 3495 1425 343 279 54 MslI CAynn'nnrTG Cuts at: 518 1166 2309 3554 3913 4072 518 Size: 648 1143 1245 359 159 2042 Fragments arranged by size: 2042 1245 1143 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2663 3012 3689 4837 5082 1606 Size: 1057 349 677 1148 245 2120 Fragments arranged by size: 2120 1148 1057 677 349 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2464 513 Size: 552 939 460 3645 Fragments arranged by size: 3645 939 552 460 NdeI CA'TA_TG Cuts at: 387 387 Size: 5596 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5596 NotI GC'GGCC_GC Cuts at: 3043 3043 Size: 5596 NspI r_CATG'y Cuts at: 1132 1229 2954 4499 1132 Size: 97 1725 1545 2229 Fragments arranged by size: 2229 1725 1545 97 PasI CC'CwG_GG Cuts at: 1348 2012 2364 1348 Size: 664 352 4580 Fragments arranged by size: 4580 664 352 PciI A'CATG_T Cuts at: 4495 4495 Size: 5596 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5596 PfoI T'CCnGG_A Cuts at: 2101 2101 Size: 5596 PmeI GTTT'AAAC Cuts at: 2900 2900 Size: 5596 PmlI CAC'GTG Cuts at: 2229 2229 Size: 5596 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3665 3697 5178 5210 3665 Size: 32 1481 32 4051 Fragments arranged by size: 4051 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2095 2506 2095 Size: 411 5185 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5596 PsiI TTA'TAA Cuts at: 3166 3166 Size: 5596 PspOMI G'GGCC_C Cuts at: 2836 2836 Size: 5596 PstI C_TGCA'G Cuts at: 830 2478 2948 830 Size: 1648 470 3478 Fragments arranged by size: 3478 1648 470 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5596 SalI G'TCGA_C Cuts at: 1936 2938 1936 Size: 1002 4594 SbfI CC_TGCA'GG Cuts at: 2948 2948 Size: 5596 ScaI AGT'ACT Cuts at: 3844 5556 3844 Size: 1712 3884 SfcI C'TryA_G Cuts at: 826 1045 2474 2944 4079 4760 4951 826 Size: 219 1429 470 1135 681 191 1471 Fragments arranged by size: 1471 1429 1135 681 470 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2168 2168 Size: 5596 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5596 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5596 SmaI CCC'GGG Cuts at: 1872 2287 2705 2923 1872 Size: 415 418 218 4545 Fragments arranged by size: 4545 418 415 218 SmlI C'TyrA_G Cuts at: 820 1017 1943 2549 3700 4601 4863 820 Size: 197 926 606 1151 901 262 1553 Fragments arranged by size: 1553 1151 926 901 606 262 197 SnaBI TAC'GTA Cuts at: 493 3387 493 Size: 2894 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5596 SphI G_CATG'C Cuts at: 1132 2954 1132 Size: 1822 3774 SspI AAT'ATT Cuts at: 5 52 3520 5 Size: 47 3468 2081 Fragments arranged by size: 3468 2081 47 StuI AGG'CCT Cuts at: 1517 2645 2832 1517 Size: 1128 187 4281 Fragments arranged by size: 4281 1128 187 StyI C'CwwG_G Cuts at: 513 1065 2004 2464 3035 513 Size: 552 939 460 571 3074 Fragments arranged by size: 3074 939 571 552 460 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2466 3623 3640 3793 3978 4423 325 Size: 1255 886 1157 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1157 886 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3842 5554 96 Size: 274 80 33 51 1443 1865 1712 138 Fragments arranged by size: 1865 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5556 5588 5556 Size: 32 5564 Tth111I GACn'n_nGTC Cuts at: 1316 2696 1316 Size: 1380 4216 XbaI T'CTAG_A Cuts at: 2932 2932 Size: 5596 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5596 XmaI C'CCGG_G Cuts at: 1870 2285 2703 2921 1870 Size: 415 418 218 4545 Fragments arranged by size: 4545 418 415 218 XmnI GAAnn'nnTTC Cuts at: 2492 3725 2492 Size: 1233 4363 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3404 276 Size: 53 83 186 936 1870 2468 Fragments arranged by size: 2468 1870 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI XcmI