################################## # NO CUT ENZYMES ( 187 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BseAI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeII MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MslI MspA1I MunI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacII SalI SapI Sau3AI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp45I Tsp509I Tth111I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AccII 86 CGCG CG^CG AluI 13 AGCT AG^CT Aor51HI 117 AGCGCT AGC^GCT AspHI 12 GAGCTC G[AT]GC[AT]^C BsaI 165 GAGACC GGTCTC(1/5) BsaWI 99 TCCGGT [AT]^CCGG[AT] BsiHKAI 12 GAGCTC G[AT]GC[AT]^C BsmAI 165 GAGAC GTCTC(1/5) BsrI 267 ACTGG ACTGG(1/-1) BstNI 235 CCAGG CC^[AT]GG BstUI 86 CGCG CG^CG BstXI 260 CCAAAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EarI 280 GAAGAG CTCTTC(1/4) Eco47III 117 AGCGCT AGC^GCT EcoRII 235 CCAGG ^CC[AT]GG FauI 69 GCGGG CCCGC(4/6) HaeII 117 AGCGCT [GA]GCGC^[CT] HphI 102 GGTGA GGTGA(8/7) Ksp632I 280 GAAGAG CTCTTC(1/4) MboII 280 GAAGA GAAGA(8/7) MvaI 235 CCAGG CC^[AT]GG MvnI 86 CGCG CG^CG PflMI 27 CCAGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 12 GAGCTC GAGCT^C SfaNI 290 GCATC GCATC(5/9) SmaI 109 CCCGGG CCC^GGG TspRI 60 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 27 CCAGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 23 CCAGCCAGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 109 CCCGGG C^CCGGG XmaI 109 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AciI 85 CCGC CCGC(-3/-1) 69 GCGG AvaI 109 CCCGGG C^[CT]CG[GA]G 170 CTCGGG BanII 12 GAGCTC G[GA]GC[CT]^C 139 GAGCCC BbvI 93 GCAGC GCAGC(8/12) 205 GCTGC BmyI 12 GAGCTC G[AGT]GC[ACT]^C 139 GAGCCC BpmI 233 CTCCAG CTGGAG(16/14) 268 CTGGAG BsmFI 112 GGGAC GGGAC(10/14) 107 GTCCC BsoBI 109 CCCGGG C^[CT]CG[GA]G 170 CTCGGG Bsp1286I 12 GAGCTC G[AGT]GC[ACT]^C 139 GAGCCC CfoI 87 GCGC GCG^C 118 GCGC Cfr13I 72 GGGCC G^G[ACGT]CC 174 GGGCC DdeI 62 CTGAG C^T[ACGT]AG 161 CTAAG EcoT14I 32 CCTTGG C^C[AT][AT]GG 247 CCAAGG HhaI 87 GCGC GCG^C 118 GCGC HinP1I 87 GCGC G^CGC 118 GCGC Sau96I 72 GGGCC G^G[ACGT]CC 174 GGGCC StyI 32 CCTTGG C^C[AT][AT]GG 247 CCAAGG TaqI 10 TCGA T^CGA 16 TCGA TseI 93 GCAGC G^C[AT]GC 205 GCTGC TthHB8I 10 TCGA T^CGA 16 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 20 enzymes) # ######################################### BcnI BsaJI BseRI BsiYI BslI BssKI Cac8I Fnu4HI HaeIII HapII HinfI HpaII ItaI MnlI MspI MwoI NciI NlaIV PleI ScrFI