(Circular) (Six-base) MAPSORT of: pFN21AE4591.seq Check: 313 from: 1 to: 8026 pFN21AE4591 8026 bp With 182 enzymes: SgfI * March 28, 2012 14:19 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3376 3376 Size: 8026 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3792 4180 4605 5211 Size: 53 83 186 936 2256 388 425 606 Cuts at: 5211 5836 278 Size: 625 2468 Fragments arranged by size: 2468 2256 936 625 606 425 388 186 83 53 AccI GT'mk_AC Cuts at: 1937 2748 4104 4395 5369 1937 Size: 811 1356 291 974 4594 Fragments arranged by size: 4594 1356 974 811 291 Acc65I G'GTAC_C Cuts at: 2604 5347 2604 Size: 2743 5283 AclI AA'CG_TT Cuts at: 1563 6153 6526 1563 Size: 4590 373 3063 Fragments arranged by size: 4590 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2855 3563 4103 6089 7473 7741 1632 Size: 1223 708 540 1986 1384 268 1917 Fragments arranged by size: 1986 1917 1384 1223 708 540 268 AfeI AGC'GCT Cuts at: 2262 4427 5153 5183 2262 Size: 2165 726 30 5105 Fragments arranged by size: 5105 2165 726 30 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7829 AflIII A'CryG_T Cuts at: 1203 6925 7902 1203 Size: 5722 977 1327 Fragments arranged by size: 5722 1327 977 AgeI A'CCGG_T Cuts at: 3185 6857 3185 Size: 3672 4354 AhdI GACnn_n'nnGTC Cuts at: 4348 6755 4348 Size: 2407 5619 AleI CACnn'nnGTG Cuts at: 1166 2487 3623 1166 Size: 1321 1136 5569 Fragments arranged by size: 5569 1321 1136 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2940 5000 5273 1184 Size: 1756 2060 273 3937 Fragments arranged by size: 3937 2060 1756 273 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2679 2711 4817 4849 2679 Size: 32 2106 32 5856 Fragments arranged by size: 5856 2106 32 32 AlwNI CAG_nnn'CTG Cuts at: 3391 3892 3982 4060 4888 5290 5769 7341 3391 Size: 501 90 78 828 402 479 1572 4076 Fragments arranged by size: 4076 1572 828 501 479 402 90 78 ApaI G_GGCC'C Cuts at: 2037 2059 2324 2349 2478 3235 2037 Size: 22 265 25 129 757 6828 Fragments arranged by size: 6828 757 265 129 25 22 ApaLI G'TGCA_C Cuts at: 6083 7239 6083 Size: 1156 6870 ApoI r'AATT_y Cuts at: 1493 5335 5561 5713 7822 1493 Size: 3842 226 152 2109 1697 Fragments arranged by size: 3842 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6580 160 Size: 872 5548 1606 Fragments arranged by size: 5548 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8026 AvaI C'yCGr_G Cuts at: 1870 1943 2036 2477 2586 3228 3875 4739 5097 Size: 73 93 441 109 642 647 864 358 Cuts at: 5097 5351 1870 Size: 254 4545 Fragments arranged by size: 4545 864 647 642 441 358 254 109 93 73 BamHI G'GATC_C Cuts at: 5356 5723 5356 Size: 367 7659 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2041 2604 3098 4050 4118 Size: 325 217 627 254 563 494 952 68 Cuts at: 4118 5347 6802 618 Size: 1229 1455 1842 Fragments arranged by size: 1842 1455 1229 952 627 563 494 325 254 217 68 BanII G_rGCy'C Cuts at: 1391 2037 2059 2283 2324 2349 2478 2808 3051 Size: 646 22 224 41 25 129 330 243 Cuts at: 3051 3235 3771 4920 5345 1391 Size: 184 536 1149 425 4072 Fragments arranged by size: 4072 1149 646 536 425 330 243 224 184 129 41 25 22 BbeI G_GCGC'C Cuts at: 4054 4122 4054 Size: 68 7958 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3953 5280 928 Size: 971 2054 1327 3674 Fragments arranged by size: 3674 2054 1327 971 BbvCI CC'TCA_GC Cuts at: 3632 4311 3632 Size: 679 7347 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4612 4646 4919 4953 6217 6251 1903 Size: 34 2675 34 273 34 1264 34 3678 Fragments arranged by size: 3678 2675 1264 273 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3682 5137 5918 7128 3682 Size: 1455 781 1210 4580 Fragments arranged by size: 4580 1455 1210 781 BclI T'GATC_A Cuts at: 1548 3180 4779 1548 Size: 1632 1599 4795 Fragments arranged by size: 4795 1632 1599 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2344 2567 2973 Size: 107 122 71 631 605 672 223 406 Cuts at: 2973 3565 4025 4293 4375 6637 136 Size: 592 460 268 82 2262 1525 Fragments arranged by size: 2262 1525 672 631 605 592 460 406 268 223 122 107 82 71 BglII A'GATC_T Cuts at: 3012 4149 8021 3012 Size: 1137 3872 3017 Fragments arranged by size: 3872 3017 1137 BlpI GC'TnA_GC Cuts at: 2942 5060 5443 2942 Size: 2118 383 5525 Fragments arranged by size: 5525 2118 383 Bme1580I G_kGCm'C Cuts at: 1790 2037 2046 2059 2324 2349 2478 2616 2717 Size: 247 9 13 265 25 129 138 101 Cuts at: 2717 3088 3235 3630 4003 6087 7243 1790 Size: 371 147 395 373 2084 1156 2573 Fragments arranged by size: 2573 2084 1156 395 373 371 265 247 147 138 129 101 25 13 9 BmgBI CAC'GTC Cuts at: 1318 2872 2887 3178 3469 1318 Size: 1554 15 291 291 5875 Fragments arranged by size: 5875 1554 291 291 15 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2562 3902 4354 Size: 416 53 83 399 24 1145 1340 452 Cuts at: 4354 5757 6715 442 Size: 1403 958 1753 Fragments arranged by size: 1753 1403 1340 1145 958 452 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8026 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2425 5173 2425 Size: 2748 5278 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2210 2822 2882 3593 4004 4166 4400 4511 Size: 971 612 60 711 411 162 234 111 Cuts at: 4511 4862 4898 5165 6686 1239 Size: 351 36 267 1521 2579 Fragments arranged by size: 2579 1521 971 711 612 411 351 267 234 162 111 60 36 Bpu10I CC'TnA_GC Cuts at: 2985 2991 3632 4311 2985 Size: 6 641 679 6700 Fragments arranged by size: 6700 679 641 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3227 6151 7016 7314 3227 Size: 2924 865 298 3939 Fragments arranged by size: 3939 2924 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4563 6689 882 Size: 617 3064 2126 2219 Fragments arranged by size: 3064 2219 2126 617 BsaAI yAC'GTr Cuts at: 493 1204 4705 4777 5817 493 Size: 711 3501 72 1040 2702 Fragments arranged by size: 3501 2702 1040 711 72 BsaBI GATnn'nnATC Cuts at: 8020 8020 Size: 8026 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3789 4051 4119 4177 Size: 53 83 186 936 2256 262 68 58 Cuts at: 4177 4461 4602 5208 5833 6215 6850 275 Size: 284 141 606 625 382 635 1451 Fragments arranged by size: 2256 1451 936 635 625 606 382 284 262 186 141 83 68 58 53 BsaWI w'CCGG_w Cuts at: 2760 2902 3185 6459 6857 7131 7278 7689 2760 Size: 142 283 3274 398 274 147 411 3097 Fragments arranged by size: 3274 3097 411 398 283 274 147 142 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2165 2195 2606 2636 3744 Size: 30 165 30 762 30 411 30 1108 Cuts at: 3744 3774 4202 4232 4259 4289 1178 Size: 30 428 30 27 30 4915 Fragments arranged by size: 4915 1108 762 428 411 165 30 30 30 30 30 30 30 27 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2123 2144 2180 2183 2186 3140 3805 4438 Size: 937 21 36 3 3 954 665 633 Cuts at: 4438 5162 1186 Size: 724 4050 Fragments arranged by size: 4050 954 937 724 665 633 36 21 3 3 BseYI C'CCAG_C Cuts at: 2323 2455 2720 2809 2813 3022 3033 3675 4641 Size: 132 265 89 4 209 11 642 966 Cuts at: 4641 4751 7229 7725 2323 Size: 110 2478 496 2624 Fragments arranged by size: 2624 2478 966 642 496 265 209 132 110 89 11 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3128 3389 3491 3867 5183 5200 1163 Size: 573 1392 261 102 376 1316 17 3989 Fragments arranged by size: 3989 1392 1316 573 376 261 102 17 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2562 2968 4471 4567 4765 5476 Size: 275 186 561 406 1503 96 198 711 Cuts at: 5476 6237 7265 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1503 1028 761 711 561 406 275 198 186 96 BsiHKAI G_wGCw'C Cuts at: 2379 2484 2585 2808 2943 4848 4989 5061 6087 Size: 105 101 223 135 1905 141 72 1026 Cuts at: 6087 6172 7243 2379 Size: 85 1071 3162 Fragments arranged by size: 3162 1905 1071 1026 223 141 135 105 101 85 72 BsmI GAATG_Cn' Cuts at: 5143 5537 5630 5143 Size: 394 93 7539 Fragments arranged by size: 7539 394 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2684 2684 Size: 8026 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2037 2046 2059 2283 2324 2349 2379 Size: 399 247 9 13 224 41 25 30 Cuts at: 2379 2478 2484 2585 2616 2717 2808 2943 3051 Size: 99 6 101 31 101 91 135 108 Cuts at: 3051 3088 3235 3630 3771 4003 4848 4920 4989 Size: 37 147 395 141 232 845 72 69 Cuts at: 4989 5061 5345 6087 6172 7243 1391 Size: 72 284 742 85 1071 2174 Fragments arranged by size: 2174 1071 845 742 399 395 284 247 232 224 147 141 135 108 101 101 99 91 85 72 72 69 41 37 31 30 25 13 9 6 BspEI T'CCGG_A Cuts at: 7689 7689 Size: 8026 BspHI T'CATG_A Cuts at: 3834 5915 7645 3834 Size: 2081 1730 4215 Fragments arranged by size: 4215 2081 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2200 2502 3141 3376 3501 3564 3648 3649 Size: 1356 302 639 235 125 63 84 1 Cuts at: 3649 3999 4416 4623 5381 844 Size: 350 417 207 758 3489 Fragments arranged by size: 3489 1356 758 639 417 350 302 235 207 125 84 63 1 BsrBI CCG'CTC Cuts at: 1394 1823 2995 3414 3577 5913 1394 Size: 429 1172 419 163 2336 3507 Fragments arranged by size: 3507 2336 1172 429 419 163 BsrDI GCAATG_nn' Cuts at: 65 1248 2922 6521 6695 65 Size: 1183 1674 3599 174 1396 Fragments arranged by size: 3599 1674 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2557 2963 3185 3278 3330 4688 6670 Size: 110 747 406 222 93 52 1358 1982 Cuts at: 6670 6857 1700 Size: 187 2869 Fragments arranged by size: 2869 1982 1358 747 406 222 187 110 93 52 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8026 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8026 BssSI C'ACGA_G Cuts at: 2660 3698 4520 5201 6086 7098 2660 Size: 1038 822 681 885 1012 3588 Fragments arranged by size: 3588 1038 1012 885 822 681 BstAPI GCAn_nnn'nTGC Cuts at: 794 3391 3694 4479 4852 5037 5046 5290 794 Size: 2597 303 785 373 185 9 244 3530 Fragments arranged by size: 3530 2597 785 373 303 244 185 9 BstBI TT'CG_AA Cuts at: 6830 6830 Size: 8026 BstEII G'GTnAC_C Cuts at: 1184 2392 2489 1184 Size: 1208 97 6721 Fragments arranged by size: 6721 1208 97 BstXI CCAn_nnnn'nTGG Cuts at: 3838 4204 4378 4801 3838 Size: 366 174 423 7063 Fragments arranged by size: 7063 423 366 174 BstYI r'GATC_y Cuts at: 1972 2230 2757 3012 3978 4149 5356 5723 6108 Size: 258 527 255 966 171 1207 367 385 Cuts at: 6108 6125 7577 7663 7675 8021 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1207 966 527 385 367 346 258 255 171 86 17 12 BstZ17I GTA'TAC Cuts at: 4105 4105 Size: 8026 Bsu36I CC'TnA_GG Cuts at: 2828 4575 5316 2828 Size: 1747 741 5538 Fragments arranged by size: 5538 1747 741 BtgI C'CryG_G Cuts at: 513 1065 2004 2059 2359 2428 2608 2643 2912 Size: 552 939 55 300 69 180 35 269 Cuts at: 2912 3600 3717 4338 513 Size: 688 117 621 4201 Fragments arranged by size: 4201 939 688 621 552 300 269 180 117 69 55 35 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3781 4331 487 Size: 683 56 2555 550 4182 Fragments arranged by size: 4182 2555 683 550 56 BtsI GCAGTG_nn' Cuts at: 2858 3560 4536 4544 5180 5542 6336 6356 2858 Size: 702 976 8 636 362 794 20 4528 Fragments arranged by size: 4528 976 794 702 636 362 20 8 ClaI AT'CG_AT Cuts at: 1554 4632 1554 Size: 3078 4948 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7991 DraI TTT'AAA Cuts at: 5330 5330 Size: 8026 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2210 7033 809 Size: 507 894 4823 1802 Fragments arranged by size: 4823 1802 894 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2021 2093 2514 Size: 54 1425 50 275 18 191 72 421 Cuts at: 2514 2559 2965 3276 3652 4026 4524 4713 4803 Size: 45 406 311 376 374 498 189 90 Cuts at: 4803 4890 5473 6362 7991 8 Size: 87 583 889 1629 43 Fragments arranged by size: 1629 1425 889 583 498 421 406 376 374 311 275 191 189 90 87 72 54 50 45 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2559 2965 5473 1537 Size: 275 747 406 2508 4090 Fragments arranged by size: 4090 2508 747 406 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3310 4001 4448 5956 1353 Size: 1957 691 447 1508 3423 Fragments arranged by size: 3423 1957 1508 691 447 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2882 6613 6987 7133 387 Size: 1064 138 1293 3731 374 146 1280 Fragments arranged by size: 3731 1293 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2806 2806 Size: 8026 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2210 2822 2855 2882 3563 3593 4004 Size: 393 578 612 33 27 681 30 411 Cuts at: 4004 4103 4166 4400 4511 4862 4898 5165 6089 Size: 99 63 234 111 351 36 267 924 Cuts at: 6089 6686 7473 7741 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 924 787 681 612 597 578 411 393 351 268 267 234 111 99 63 36 33 30 27 EcoNI CCTnn'n_nnAGG Cuts at: 2687 3137 3902 4928 5201 2687 Size: 450 765 1026 273 5512 Fragments arranged by size: 5512 1026 765 450 273 EcoO109I rG'GnC_Cy Cuts at: 2033 2065 2113 2212 2345 2346 2998 3130 3921 Size: 32 48 99 133 1 652 132 791 Cuts at: 3921 4057 4486 4735 4825 5266 2033 Size: 136 429 249 90 441 4793 Fragments arranged by size: 4793 791 652 441 429 249 136 133 132 99 90 48 32 1 EcoRI G'AATT_C Cuts at: 5335 5713 5335 Size: 378 7648 EcoRV GAT'ATC Cuts at: 2953 4636 2953 Size: 1683 6343 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4414 4414 Size: 8026 FseI GG_CCGG'CC Cuts at: 2561 3282 2561 Size: 721 7305 FspI TGC'GCA Cuts at: 2636 4502 4907 5249 6532 2636 Size: 1866 405 342 1283 4130 Fragments arranged by size: 4130 1866 1283 405 342 HaeII r_GCGC'y Cuts at: 1678 2264 2462 2651 3068 4054 4082 4095 4122 Size: 586 198 189 417 986 28 13 27 Cuts at: 4122 4429 5155 5185 5299 7173 1678 Size: 307 726 30 114 1874 2531 Fragments arranged by size: 2531 1874 986 726 586 417 307 198 189 114 30 28 27 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2165 2197 3744 3776 3800 Size: 32 135 32 593 32 1547 32 24 Cuts at: 3800 3832 3892 3924 4259 4291 4334 4366 4592 Size: 32 60 32 335 32 43 32 226 Cuts at: 4592 4624 6667 6699 6741 6773 1373 Size: 32 2043 32 42 32 2626 Fragments arranged by size: 2626 2043 1547 593 335 226 135 60 43 42 32 32 32 32 32 32 32 32 32 32 32 24 HincII GTy'rAC Cuts at: 669 1657 1938 2749 5370 5616 669 Size: 988 281 811 2621 246 3079 Fragments arranged by size: 3079 2621 988 811 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8026 HpaI GTT'AAC Cuts at: 5616 5616 Size: 8026 KasI G'GCGC_C Cuts at: 4050 4118 4050 Size: 68 7958 KpnI G_GTAC'C Cuts at: 2608 5351 2608 Size: 2743 5283 MfeI C'AATT_G Cuts at: 5625 5625 Size: 8026 MluI A'CGCG_T Cuts at: 7902 7902 Size: 8026 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2280 2317 3315 4704 4800 7140 7324 1305 Size: 975 37 998 1389 96 2340 184 2007 Fragments arranged by size: 2340 2007 1389 998 975 184 96 37 MscI TGG'CCA Cuts at: 10 64 1489 1832 2516 3654 4028 4526 4892 Size: 54 1425 343 684 1138 374 498 366 Cuts at: 4892 10 Size: 3144 Fragments arranged by size: 3144 1425 1138 684 498 374 366 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2487 2642 2699 3623 3836 5136 5984 Size: 648 1321 155 57 924 213 1300 848 Cuts at: 5984 6343 6502 518 Size: 359 159 2042 Fragments arranged by size: 2042 1321 1300 924 848 648 359 213 159 155 57 MspA1I CmG'CkG Cuts at: 1606 2813 2860 3679 4075 4204 4340 4645 4831 Size: 1207 47 819 396 129 136 305 186 Cuts at: 4831 4888 5442 6119 7267 7512 1606 Size: 57 554 677 1148 245 2120 Fragments arranged by size: 2120 1207 1148 819 677 554 396 305 245 186 136 129 57 47 NaeI GCC'GGC Cuts at: 2559 2965 3280 3332 4690 2559 Size: 406 315 52 1358 5895 Fragments arranged by size: 5895 1358 406 315 52 NarI GG'CG_CC Cuts at: 4051 4119 4051 Size: 68 7958 NcoI C'CATG_G Cuts at: 513 1065 2004 2912 513 Size: 552 939 908 5627 Fragments arranged by size: 5627 939 908 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8026 NgoMIV G'CCGG_C Cuts at: 2557 2963 3278 3330 4688 2557 Size: 406 315 52 1358 5895 Fragments arranged by size: 5895 1358 406 315 52 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8026 NotI GC'GGCC_GC Cuts at: 5473 5473 Size: 8026 NspI r_CATG'y Cuts at: 1132 1229 2599 3382 5248 5384 6929 1132 Size: 97 1370 783 1866 136 1545 2229 Fragments arranged by size: 2229 1866 1545 1370 783 136 97 PasI CC'CwG_GG Cuts at: 1348 2030 4324 1348 Size: 682 2294 5050 Fragments arranged by size: 5050 2294 682 PciI A'CATG_T Cuts at: 6925 6925 Size: 8026 PflMI CCAn_nnn'nTGG Cuts at: 1108 2299 2317 3448 3982 4060 1108 Size: 1191 18 1131 534 78 5074 Fragments arranged by size: 5074 1191 1131 534 78 18 PfoI T'CCnGG_A Cuts at: 2187 2682 3297 3981 4059 2187 Size: 495 615 684 78 6154 Fragments arranged by size: 6154 684 615 495 78 PmeI GTTT'AAAC Cuts at: 5330 5330 Size: 8026 PmlI CAC'GTG Cuts at: 4705 4705 Size: 8026 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2747 2779 3896 3928 6095 6127 7608 7640 2747 Size: 32 1117 32 2167 32 1481 32 3133 Fragments arranged by size: 3133 2167 1481 1117 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2113 2212 2998 3130 3921 4057 4735 4825 5266 Size: 99 786 132 791 136 678 90 441 Cuts at: 5266 2113 Size: 4873 Fragments arranged by size: 4873 791 786 678 441 136 132 99 90 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8026 PsiI TTA'TAA Cuts at: 5596 5596 Size: 8026 PspOMI G'GGCC_C Cuts at: 2033 2055 2320 2345 2474 3231 2033 Size: 22 265 25 129 757 6828 Fragments arranged by size: 6828 757 265 129 25 22 PstI C_TGCA'G Cuts at: 830 2157 2181 3141 3387 3498 5378 830 Size: 1327 24 960 246 111 1880 3478 Fragments arranged by size: 3478 1880 1327 960 246 111 24 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8026 PvuII CAG'CTG Cuts at: 2813 2860 3679 4075 4204 4645 4888 2813 Size: 47 819 396 129 441 243 5951 Fragments arranged by size: 5951 819 441 396 243 129 47 SacI G_AGCT'C Cuts at: 2808 2808 Size: 8026 SacII CC_GC'GG Cuts at: 4341 4341 Size: 8026 SalI G'TCGA_C Cuts at: 1936 2747 5368 1936 Size: 811 2621 4594 Fragments arranged by size: 4594 2621 811 SanDI GG'GwC_CC Cuts at: 2998 4735 2998 Size: 1737 6289 SapI GCTCTTCn'nnn_ Cuts at: 4448 4448 Size: 8026 SbfI CC_TGCA'GG Cuts at: 5378 5378 Size: 8026 ScaI AGT'ACT Cuts at: 4871 6274 7986 4871 Size: 1403 1712 4911 Fragments arranged by size: 4911 1712 1403 SexAI A'CCwGG_T Cuts at: 4530 4530 Size: 8026 SfcI C'TryA_G Cuts at: 826 1045 2153 2177 3137 3383 3494 4237 5374 Size: 219 1108 24 960 246 111 743 1137 Cuts at: 5374 6509 7190 7381 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1137 1135 1108 960 743 681 246 219 191 111 24 SfiI GGCCn_nnn'nGGCC Cuts at: 4025 4025 Size: 8026 SfoI GGC'GCC Cuts at: 4052 4120 4052 Size: 68 7958 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8026 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8026 SmaI CCC'GGG Cuts at: 1872 2038 3877 4741 5099 5353 1872 Size: 166 1839 864 358 254 4545 Fragments arranged by size: 4545 1839 864 358 254 166 SmlI C'TyrA_G Cuts at: 820 1017 1943 3242 6130 7031 7293 820 Size: 197 926 1299 2888 901 262 1553 Fragments arranged by size: 2888 1553 1299 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5817 493 Size: 5324 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8026 SphI G_CATG'C Cuts at: 1132 5384 1132 Size: 4252 3774 SspI AAT'ATT Cuts at: 5 52 5950 5 Size: 47 5898 2081 Fragments arranged by size: 5898 2081 47 StuI AGG'CCT Cuts at: 1517 2709 3143 5321 1517 Size: 1192 434 2178 4222 Fragments arranged by size: 4222 2178 1192 434 StyI C'CwwG_G Cuts at: 513 1065 2004 2912 3102 3342 4014 4029 4251 Size: 552 939 908 190 240 672 15 222 Cuts at: 4251 4656 4794 5465 513 Size: 405 138 671 3074 Fragments arranged by size: 3074 939 908 672 671 552 405 240 222 190 138 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2477 2913 3086 4336 4581 6053 6070 Size: 1255 897 436 173 1250 245 1472 17 Cuts at: 6070 6223 6408 6853 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1472 1255 1250 897 445 436 245 185 173 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3402 3534 4218 Size: 274 80 33 51 1443 1425 132 684 Cuts at: 4218 4869 6272 7984 96 Size: 651 1403 1712 138 Fragments arranged by size: 1712 1443 1425 1403 684 651 274 138 132 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2606 2638 2861 2893 4604 4636 7986 8018 2606 Size: 32 223 32 1711 32 3350 32 2614 Fragments arranged by size: 3350 2614 1711 223 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2783 2888 3002 4277 4766 1316 Size: 1467 105 114 1275 489 4576 Fragments arranged by size: 4576 1467 1275 489 114 105 XbaI T'CTAG_A Cuts at: 5362 5362 Size: 8026 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3802 4021 4061 4204 4252 3802 Size: 219 40 143 48 7576 Fragments arranged by size: 7576 219 143 48 40 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8026 XmaI C'CCGG_G Cuts at: 1870 2036 3875 4739 5097 5351 1870 Size: 166 1839 864 358 254 4545 Fragments arranged by size: 4545 1839 864 358 254 166 XmnI GAAnn'nnTTC Cuts at: 6155 6155 Size: 8026 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3790 4178 4603 5209 Size: 53 83 186 936 2256 388 425 606 Cuts at: 5209 5834 276 Size: 625 2468 Fragments arranged by size: 2468 2256 936 625 606 425 388 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BsiWI DraIII FspAI NruI NsiI PacI PspXI PsrI RsrII SrfI SwaI