################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI Alw44I AlwI AocI ApaI ApaLI AseI AsnI Asp718I AspEI AvaII AviII AvrII BalI BamHI BbsI BbvCI BcgI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI FspI HgaI HincII HindII HindIII HpaI KpnI MamI MfeI MflI MluI MluNI MroI MscI MunI NcoI NotI NruI NsiI NspI NspV PacI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SalI SapI SbfI ScaI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TfiI Van91I XbaI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AcsI 874 AAATTT [GA]^AATT[CT] AcyI 11 GGCGCC G[GA]^CG[CT]C AflIII 1028 ACACGT A^C[GA][CT]GT ApoI 874 AAATTT [GA]^AATT[CT] AscI 71 GGCGCGCC GG^CGCGCC Asp700I 1069 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 982 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 11 GGCGCC GGCGC^C BbrPI 1029 CACGTG CAC^GTG BclI 972 TGATCA T^GATCA BfaI 34 CTAG C^TAG BsaAI 1029 CACGTG [CT]AC^GT[GA] BsaHI 11 GGCGCC G[GA]^CG[CT]C BsaI 1195 GAGACC GGTCTC(1/5) BsaWI 864 TCCGGT [AT]^CCGG[AT] BseRI 1192 GAGGAG GAGGAG(10/8) BsrBI 127 CCGCTC CCGCTC(-3/-3) FbaI 972 TGATCA T^GATCA Hin1I 11 GGCGCC G[GA]^CG[CT]C HinfI 133 GAGTC G^A[ACGT]TC KasI 11 GGCGCC G^GCGCC KspI 23 CCGCGG CCGC^GG MaeI 34 CTAG C^TAG MaeII 1030 ACGT A^CGT MaeIII 1090 GTGAC ^GT[ACGT]AC NaeI 292 GCCGGC GCC^GGC NarI 11 GGCGCC GG^CGCC NdeI 614 CATATG CA^TATG NgoMIV 292 GCCGGC G^CCGGC NheI 33 GCTAGC G^CTAGC PaeR7I 130 CTCGAG C^TCGAG PflFI 982 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 133 GAGTC GAGTC(4/5) PmaCI 1029 CACGTG CAC^GTG PmlI 1029 CACGTG CAC^GTG SacII 23 CCGCGG CCGC^GG SexAI 1001 ACCTGGT A^CC[AT]GGT SfoI 11 GGCGCC GGC^GCC StuI 242 AGGCCT AGG^CCT TaqI 131 TCGA T^CGA Tsp45I 1090 GTGAC ^GT[GC]AC Tsp509I 875 AATT ^AATT Tth111I 982 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 131 TCGA T^CGA XcmI 674 CCATCACAGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 130 CTCGAG C^TCGAG XmnI 1069 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AfaI 162 GTAC GT^AC 1017 GTAC AlwNI 370 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG 997 CAGAACCTG Aor51HI 36 AGCGCT AGC^GCT 359 AGCGCT AspHI 172 GAGCAC G[AT]GC[AT]^C 1048 GAGCAC BanI 11 GGCGCC G^G[CT][GA]CC 577 GGTGCC BanII 283 GAGCCC G[GA]GC[CT]^C 436 GAGCCC BsgI 217 CTGCAC GTGCAG(16/14) 950 GTGCAG BsiHKAI 172 GAGCAC G[AT]GC[AT]^C 1048 GAGCAC BsmAI 1195 GAGAC GTCTC(1/5) 728 GTCTC BsmI 814 GAATGC GAATGC(1/-1) 936 GAATGC BsrFI 143 ACCGGC [GA]^CCGG[CT] 292 GCCGGC BstAPI 219 GCACCGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 369 GCAGGGCCTGC Cfr10I 143 ACCGGC [GA]^CCGG[CT] 292 GCCGGC DdeI 1041 CTCAG C^T[ACGT]AG 1124 CTAAG DsaI 23 CCGCGG C^C[GA][CT]GG 109 CCACGG Eco47III 36 AGCGCT AGC^GCT 359 AGCGCT FauI 286 CCCGC CCCGC(4/6) 642 GCGGG HphI 655 GGTGA GGTGA(8/7) 842 TCACC MseI 593 TTAA T^TAA 1085 TTAA MslI 523 CATCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 678 CACAGACATG PstI 261 CTGCAG CTGCA^G 451 CTGCAG RsaI 162 GTAC GT^AC 1017 GTAC SfaNI 123 GATGC GCATC(5/9) 522 GCATC SmlI 130 CTCGAG C^T[CT][GA]AG 355 CTCAAG Tru9I 593 TTAA T^TAA 1085 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AccII AciI AluI AvaI BbvI BcnI BmyI BpmI BsaJI BsiYI BslI BsmFI BsoBI Bsp1286I BsrI BssHII BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DpnII DraII EarI EcoO109I EcoRII Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII ItaI Ksp632I MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfcI TseI TspRI