(Linear) (Six-base) MAPSORT of: KIEE6320.seq Check: 8461 from: 1 to: 1203 KIEE6320.orf With 182 enzymes: SgfI * March 16, 2015 11:29 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 538 952 1088 1203 Size: 538 414 136 115 Fragments arranged by size: 538 414 136 115 AfeI AGC'GCT Cuts at: 0 38 361 1203 Size: 38 323 842 Fragments arranged by size: 842 323 38 AflIII A'CryG_T Cuts at: 0 1028 1203 Size: 1028 175 AlwNI CAG_nnn'CTG Cuts at: 0 375 1002 1203 Size: 375 627 201 Fragments arranged by size: 627 375 201 ApoI r'AATT_y Cuts at: 0 874 1203 Size: 874 329 AscI GG'CGCG_CC Cuts at: 0 72 1203 Size: 72 1131 AvaI C'yCGr_G Cuts at: 0 130 280 310 1203 Size: 130 150 30 893 Fragments arranged by size: 893 150 130 30 BanI G'GyrC_C Cuts at: 0 11 577 1203 Size: 11 566 626 Fragments arranged by size: 626 566 11 BanII G_rGCy'C Cuts at: 0 287 440 1203 Size: 287 153 763 Fragments arranged by size: 763 287 153 BbeI G_GCGC'C Cuts at: 0 15 1203 Size: 15 1188 BclI T'GATC_A Cuts at: 0 972 1203 Size: 972 231 BmrI ACTGGGnnnn_n' Cuts at: 0 186 841 1203 Size: 186 655 362 Fragments arranged by size: 655 362 186 BmtI G_CTAG'C Cuts at: 0 37 1203 Size: 37 1166 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 220 684 1084 1203 Size: 220 464 400 119 Fragments arranged by size: 464 400 220 119 Bpu10I CC'TnA_GC Cuts at: 0 1124 1203 Size: 1124 79 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 340 1203 Size: 340 863 BsaI GGTCTCn'nnnn_ Cuts at: 0 1189 1203 Size: 1189 14 BsaAI yAC'GTr Cuts at: 0 1031 1203 Size: 1031 172 BsaHI Gr'CG_yC Cuts at: 0 12 1203 Size: 12 1191 BsaWI w'CCGG_w Cuts at: 0 864 1203 Size: 864 339 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1145 1175 1203 Size: 1145 30 28 Fragments arranged by size: 1145 30 28 BseYI C'CCAG_C Cuts at: 0 395 400 1203 Size: 395 5 803 Fragments arranged by size: 803 395 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 202 971 1203 Size: 202 769 232 Fragments arranged by size: 769 232 202 BsiHKAI G_wGCw'C Cuts at: 0 176 1052 1203 Size: 176 876 151 Fragments arranged by size: 876 176 151 BsmI GAATG_Cn' Cuts at: 0 820 942 1203 Size: 820 122 261 Fragments arranged by size: 820 261 122 Bsp1286I G_dGCh'C Cuts at: 0 176 287 440 1052 1203 Size: 176 111 153 612 151 Fragments arranged by size: 612 176 153 151 111 BsrBI CCG'CTC Cuts at: 0 129 1203 Size: 129 1074 BsrFI r'CCGG_y Cuts at: 0 143 292 1203 Size: 143 149 911 Fragments arranged by size: 911 149 143 BssHII G'CGCG_C Cuts at: 0 56 63 72 344 1203 Size: 56 7 9 272 859 Fragments arranged by size: 859 272 56 9 7 BstAPI GCAn_nnn'nTGC Cuts at: 0 225 375 1203 Size: 225 150 828 Fragments arranged by size: 828 225 150 BtgI C'CryG_G Cuts at: 0 23 109 1203 Size: 23 86 1094 Fragments arranged by size: 1094 86 23 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 422 477 1203 Size: 422 55 726 Fragments arranged by size: 726 422 55 BtsI GCAGTG_nn' Cuts at: 0 348 644 1203 Size: 348 296 559 Fragments arranged by size: 559 348 296 EarI CTCTTCn'nnn_ Cuts at: 0 328 538 827 1203 Size: 328 210 289 376 Fragments arranged by size: 376 328 289 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 376 433 1203 Size: 376 57 770 Fragments arranged by size: 770 376 57 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 220 538 684 952 1084 1088 1203 Size: 220 318 146 268 132 4 115 Fragments arranged by size: 318 268 220 146 132 115 4 EcoO109I rG'GnC_Cy Cuts at: 0 330 372 1035 1203 Size: 330 42 663 168 Fragments arranged by size: 663 330 168 42 HaeII r_GCGC'y Cuts at: 0 15 31 40 269 363 432 1203 Size: 15 16 9 229 94 69 771 Fragments arranged by size: 771 229 94 69 16 15 9 KasI G'GCGC_C Cuts at: 0 11 1203 Size: 11 1192 MslI CAynn'nnrTG Cuts at: 0 527 682 1203 Size: 527 155 521 Fragments arranged by size: 527 521 155 MspA1I CmG'CkG Cuts at: 0 25 167 499 1203 Size: 25 142 332 704 Fragments arranged by size: 704 332 142 25 NaeI GCC'GGC Cuts at: 0 294 1203 Size: 294 909 NarI GG'CG_CC Cuts at: 0 12 1203 Size: 12 1191 NdeI CA'TA_TG Cuts at: 0 615 1203 Size: 615 588 NgoMIV G'CCGG_C Cuts at: 0 292 1203 Size: 292 911 NheI G'CTAG_C Cuts at: 0 33 1203 Size: 33 1170 PmlI CAC'GTG Cuts at: 0 1031 1203 Size: 1031 172 PsiI TTA'TAA Cuts at: 0 563 917 1203 Size: 563 354 286 Fragments arranged by size: 563 354 286 PspXI vC'TCGA_Gb Cuts at: 0 130 1203 Size: 130 1073 PstI C_TGCA'G Cuts at: 0 265 455 1203 Size: 265 190 748 Fragments arranged by size: 748 265 190 SacII CC_GC'GG Cuts at: 0 26 1203 Size: 26 1177 SexAI A'CCwGG_T Cuts at: 0 1001 1203 Size: 1001 202 SfcI C'TryA_G Cuts at: 0 90 261 451 1203 Size: 90 171 190 752 Fragments arranged by size: 752 190 171 90 SfoI GGC'GCC Cuts at: 0 13 1203 Size: 13 1190 SmlI C'TyrA_G Cuts at: 0 130 355 1203 Size: 130 225 848 Fragments arranged by size: 848 225 130 StuI AGG'CCT Cuts at: 0 244 1203 Size: 244 959 TatI w'GTAC_w Cuts at: 0 161 1203 Size: 161 1042 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1145 1177 1203 Size: 1145 32 26 Fragments arranged by size: 1145 32 26 Tth111I GACn'n_nGTC Cuts at: 0 985 1203 Size: 985 218 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 681 1203 Size: 681 522 XhoI C'TCGA_G Cuts at: 0 130 1203 Size: 130 1073 XmnI GAAnn'nnTTC Cuts at: 0 1073 1203 Size: 1073 130 Enzymes that do cut: AcuI AfeI AflIII AlwNI ApoI AscI AvaI BanI BanII BbeI BclI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I BsrBI BsrFI BssHII BstAPI BtgI BtgZI BtsI EarI EciI Eco57MI EcoO109I HaeII KasI MslI MspA1I NaeI NarI NdeI NgoMIV NheI PmlI PsiI PspXI PstI SacII SexAI SfcI SfoI SmlI StuI TatI TstI Tth111I XcmI XhoI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI BplI BsaBI BseRI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrDI BsrGI BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EaeI EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI NcoI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI PsrI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StyI SwaI TaqII XbaI XmaI ZraI