################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BbeI BbrPI BbvCI BcgI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MroI MslI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccI 529 GTAGAC GT^[AC][GT]AC AccII 509 CGCG CG^CG AlwNI 586 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 881 AGCGCT AGC^GCT AviII 1223 TGCGCA TGC^GCA BalI 821 TGGCCA TGG^CCA BamHI 647 GGATCC G^GATCC BbsI 696 GAAGAC GAAGAC(2/6) BclI 368 TGATCA T^GATCA BfaI 799 CTAG C^TAG BpuAI 696 GAAGAC GAAGAC(2/6) BsaI 443 GAGACC GGTCTC(1/5) BsiEI 53 CGACCG CG[GA][CT]^CG BsmBI 484 GAGACG CGTCTC(1/5) BspHI 161 TCATGA T^CATGA BsrBI 1029 CCGCTC CCGCTC(-3/-3) BssSI 1249 CACGAG CACGAG(-5/-1) BstUI 509 CGCG CG^CG BstXI 945 CCAGCTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DdeI 731 CTAAG C^T[ACGT]AG EaeI 821 TGGCCA [CT]^GGCC[GA] EarI 165 GAAGAG CTCTTC(1/4) Eco47III 881 AGCGCT AGC^GCT FbaI 368 TGATCA T^GATCA FspI 1223 TGCGCA TGC^GCA HgaI 486 GACGC GACGC(5/10) Ksp632I 165 GAAGAG CTCTTC(1/4) MaeI 799 CTAG C^TAG MluNI 821 TGGCCA TGG^CCA MscI 821 TGGCCA TGG^CCA MseI 977 TTAA T^TAA MvnI 509 CGCG CG^CG NcoI 521 CCATGG C^CATGG PflMI 585 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 161 TCATGA T^CATGA SexAI 446 ACCTGGT A^CC[AT]GGT SmlI 100 CTCAAG C^T[CT][GA]AG TaqI 281 TCGA T^CGA Tru9I 977 TTAA T^TAA TthHB8I 281 TCGA T^CGA Van91I 585 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AfaI 261 GTAC GT^AC 393 GTAC ApaI 679 GGGCCC GGGCC^C 1178 GGGCCC AvaII 906 GGACC G^G[AT]CC 1014 GGACC BsaBI 153 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 292 GATGTTGATC BsmFI 688 GGGAC GGGAC(10/14) 905 GGGAC BsrI 28 CCAGT ACTGG(1/-1) 1182 CCAGT DraII 679 GGGCCCC [GA]G^G[ACGT]CC[CT] 1177 GGGGCCC EcoNI 655 CCTCCAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 819 CCTGGCCAAGG EcoO109I 679 GGGCCCC [GA]G^G[ACGT]CC[CT] 1177 GGGGCCC EcoT14I 521 CCATGG C^C[AT][AT]GG 824 CCAAGG HaeII 834 AGCGCC [GA]GCGC^[CT] 881 AGCGCT MamI 153 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 292 GATGTTGATC PvuII 763 CAGCTG CAG^CTG 946 CAGCTG RsaI 261 GTAC GT^AC 393 GTAC SmaI 710 CCCGGG CCC^GGG 1082 CCCGGG SspI 16 AATATT AAT^ATT 328 AATATT StyI 521 CCATGG C^C[AT][AT]GG 824 CCAAGG TfiI 3 GAATC G^A[AT]TC 1091 GAATC Tsp509I 84 AATT ^AATT 984 AATT XmaCI 710 CCCGGG C^CCGGG 1082 CCCGGG XmaI 710 CCCGGG C^CCGGG 1082 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AciI AluI AlwI AspHI AvaI BanI BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsoBI Bsp1286I BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DpnII DsaI EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI SacI Sau3AI Sau96I ScrFI SfaNI TseI Tsp45I TspRI XcmI XhoII