(Circular) (Six-base) MAPSORT of: pFN21AE4424.seq Check: 3377 from: 1 to: 6022 pFN21AE4424 6022 bp With 182 enzymes: SgfI * March 28, 2012 13:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3832 278 Size: 53 83 186 936 2296 2468 Fragments arranged by size: 2468 2296 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2535 3365 1937 Size: 598 830 4594 Fragments arranged by size: 4594 830 598 Acc65I G'GTAC_C Cuts at: 3343 3343 Size: 6022 AclI AA'CG_TT Cuts at: 1563 4149 4522 1563 Size: 2586 373 3063 Fragments arranged by size: 3063 2586 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4085 5469 5737 1632 Size: 2453 1384 268 1917 Fragments arranged by size: 2453 1917 1384 268 AfeI AGC'GCT Cuts at: 2888 2888 Size: 6022 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5825 AflIII A'CryG_T Cuts at: 1203 4921 5898 1203 Size: 3718 977 1327 Fragments arranged by size: 3718 1327 977 AgeI A'CCGG_T Cuts at: 4853 4853 Size: 6022 AhdI GACnn_n'nnGTC Cuts at: 4751 4751 Size: 6022 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6022 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2159 1184 Size: 975 5047 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2456 2488 2456 Size: 32 5990 AlwNI CAG_nnn'CTG Cuts at: 2596 3765 5337 2596 Size: 1169 1572 3281 Fragments arranged by size: 3281 1572 1169 ApaI G_GGCC'C Cuts at: 2688 3187 2688 Size: 499 5523 ApaLI G'TGCA_C Cuts at: 4079 5235 4079 Size: 1156 4866 ApoI r'AATT_y Cuts at: 1493 3331 3557 3709 5818 1493 Size: 1838 226 152 2109 1697 Fragments arranged by size: 2109 1838 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4576 160 Size: 872 3544 1606 Fragments arranged by size: 3544 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6022 AvaI C'yCGr_G Cuts at: 1870 1943 2715 3037 3087 3347 1870 Size: 73 772 322 50 260 4545 Fragments arranged by size: 4545 772 322 260 73 50 BamHI G'GATC_C Cuts at: 2652 3352 3719 2652 Size: 700 367 4955 Fragments arranged by size: 4955 700 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2327 2795 2879 3236 3343 Size: 325 217 627 540 468 84 357 107 Cuts at: 3343 4798 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 540 468 357 325 217 107 84 BanII G_rGCy'C Cuts at: 1391 2202 2292 2688 3187 3249 3341 1391 Size: 811 90 396 499 62 92 4072 Fragments arranged by size: 4072 811 499 396 92 90 62 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2708 928 Size: 971 809 4242 Fragments arranged by size: 4242 971 809 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4213 4247 1903 Size: 34 2276 34 3678 Fragments arranged by size: 3678 2276 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3914 5124 3914 Size: 1210 4812 BclI T'GATC_A Cuts at: 1548 2373 1548 Size: 825 5197 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4633 136 Size: 107 122 71 631 605 2961 1525 Fragments arranged by size: 2961 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6017 6017 Size: 6022 BlpI GC'TnA_GC Cuts at: 3439 3439 Size: 6022 Bme1580I G_kGCm'C Cuts at: 1790 2688 3187 4083 5239 1790 Size: 898 499 896 1156 2573 Fragments arranged by size: 2573 1156 898 896 499 BmgBI CAC'GTC Cuts at: 1318 3139 1318 Size: 1821 4201 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3181 3753 4711 Size: 416 53 83 399 24 1764 572 958 Cuts at: 4711 442 Size: 1753 Fragments arranged by size: 1764 1753 958 572 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6022 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2527 2527 Size: 6022 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2507 2957 3299 4682 1239 Size: 1268 450 342 1383 2579 Fragments arranged by size: 2579 1383 1268 450 342 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2090 4147 5012 5310 2090 Size: 2057 865 298 2802 Fragments arranged by size: 2802 2057 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2442 4685 882 Size: 617 943 2243 2219 Fragments arranged by size: 2243 2219 943 617 BsaAI yAC'GTr Cuts at: 493 1204 3813 493 Size: 711 2609 2702 Fragments arranged by size: 2702 2609 711 BsaBI GATnn'nnATC Cuts at: 2162 2301 6016 2162 Size: 139 3715 2168 Fragments arranged by size: 3715 2168 139 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3829 4211 4846 275 Size: 53 83 186 936 2296 382 635 1451 Fragments arranged by size: 2296 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4455 4853 5127 5274 5685 4455 Size: 398 274 147 411 4792 Fragments arranged by size: 4792 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2258 2288 2330 2360 2439 Size: 30 165 30 855 30 42 30 79 Cuts at: 2439 2456 2469 2486 1178 Size: 17 13 17 4714 Fragments arranged by size: 4714 855 165 79 42 30 30 30 30 17 17 13 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2111 2135 2333 3020 3095 3164 1186 Size: 925 24 198 687 75 69 4044 Fragments arranged by size: 4044 925 687 198 75 69 24 BseYI C'CCAG_C Cuts at: 2766 2906 5225 5721 2766 Size: 140 2319 496 3067 Fragments arranged by size: 3067 2319 496 140 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2795 2942 3148 3305 1163 Size: 573 1059 147 206 157 3880 Fragments arranged by size: 3880 1059 573 206 157 147 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2061 3472 4233 5261 1540 Size: 275 186 60 1411 761 1028 2301 Fragments arranged by size: 2301 1411 1028 761 275 186 60 BsiHKAI G_wGCw'C Cuts at: 2106 2202 2292 3249 4083 4168 5239 2106 Size: 96 90 957 834 85 1071 2889 Fragments arranged by size: 2889 1071 957 834 96 90 85 BsmI GAATG_Cn' Cuts at: 3533 3626 3533 Size: 93 5929 BsmBI CGTCTCn'nnnn_ Cuts at: 2483 2483 Size: 6022 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2106 2202 2292 2688 3187 3249 3341 Size: 399 316 96 90 396 499 62 92 Cuts at: 3341 4083 4168 5239 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 499 399 396 316 96 92 90 85 62 BspEI T'CCGG_A Cuts at: 5685 5685 Size: 6022 BspHI T'CATG_A Cuts at: 2166 3911 5641 2166 Size: 1745 1730 2547 Fragments arranged by size: 2547 1745 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3377 844 Size: 2533 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3036 3909 1394 Size: 429 1213 873 3507 Fragments arranged by size: 3507 1213 873 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4517 4691 65 Size: 1183 3269 174 1396 Fragments arranged by size: 3269 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4666 4853 1700 Size: 110 2856 187 2869 Fragments arranged by size: 2869 2856 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6022 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6022 BssSI C'ACGA_G Cuts at: 3254 4082 5094 3254 Size: 828 1012 4182 Fragments arranged by size: 4182 1012 828 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6022 BstBI TT'CG_AA Cuts at: 4826 4826 Size: 6022 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6022 BstXI CCAn_nnnn'nTGG Cuts at: 2957 2957 Size: 6022 BstYI r'GATC_y Cuts at: 1972 2586 2652 3105 3352 3719 4104 4121 5573 Size: 614 66 453 247 367 385 17 1452 Cuts at: 5573 5659 5671 6017 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 614 453 385 367 346 247 86 66 17 12 BtgI C'CryG_G Cuts at: 513 1065 2115 2526 2547 3240 513 Size: 552 1050 411 21 693 3295 Fragments arranged by size: 3295 1050 693 552 411 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5283 Fragments arranged by size: 5283 683 56 BtsI GCAGTG_nn' Cuts at: 3224 3538 4332 4352 3224 Size: 314 794 20 4894 Fragments arranged by size: 4894 794 314 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6022 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2988 3023 529 Size: 35 2424 35 3528 Fragments arranged by size: 3528 2424 35 35 DraI TTT'AAA Cuts at: 3326 3326 Size: 6022 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5029 809 Size: 507 3713 1802 Fragments arranged by size: 3713 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2826 3469 4358 Size: 54 1425 50 275 18 996 643 889 Cuts at: 4358 5987 8 Size: 1629 43 Fragments arranged by size: 1629 1425 996 889 643 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3469 1537 Size: 275 1657 4090 Fragments arranged by size: 4090 1657 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2165 3952 1353 Size: 812 1787 3423 Fragments arranged by size: 3423 1787 812 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4609 4983 5129 387 Size: 1064 138 3020 374 146 1280 Fragments arranged by size: 3020 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2200 2290 3247 2200 Size: 90 957 4975 Fragments arranged by size: 4975 957 90 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2507 2957 3299 4085 4682 5469 5737 Size: 393 875 450 342 786 597 787 268 Cuts at: 5737 1239 Size: 1524 Fragments arranged by size: 1524 875 787 786 597 450 393 342 268 EcoNI CCTnn'n_nnAGG Cuts at: 2664 2828 2664 Size: 164 5858 EcoO109I rG'GnC_Cy Cuts at: 2685 3183 2685 Size: 498 5524 EcoRI G'AATT_C Cuts at: 3331 3709 3331 Size: 378 5644 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2761 2761 Size: 6022 FspI TGC'GCA Cuts at: 3230 4528 3230 Size: 1298 4724 HaeII r_GCGC'y Cuts at: 1678 2843 2890 5169 1678 Size: 1165 47 2279 2531 Fragments arranged by size: 2531 2279 1165 47 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2330 2362 2644 2676 4663 Size: 32 135 32 758 32 282 32 1987 Cuts at: 4663 4695 4737 4769 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1987 758 282 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3366 3612 669 Size: 988 281 1428 246 3079 Fragments arranged by size: 3079 1428 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6022 HpaI GTT'AAC Cuts at: 3612 3612 Size: 6022 KpnI G_GTAC'C Cuts at: 3347 3347 Size: 6022 MfeI C'AATT_G Cuts at: 3621 3621 Size: 6022 MluI A'CGCG_T Cuts at: 5898 5898 Size: 6022 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2301 2680 2847 5136 5320 1305 Size: 996 379 167 2289 184 2007 Fragments arranged by size: 2289 2007 996 379 184 167 MscI TGG'CCA Cuts at: 10 64 1489 1832 2828 10 Size: 54 1425 343 996 3204 Fragments arranged by size: 3204 1425 996 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3980 4339 4498 518 Size: 648 2814 359 159 2042 Fragments arranged by size: 2814 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2770 2953 3162 3438 4115 5263 5508 1606 Size: 1164 183 209 276 677 1148 245 2120 Fragments arranged by size: 2120 1164 1148 677 276 245 209 183 NcoI C'CATG_G Cuts at: 513 1065 2526 513 Size: 552 1461 4009 Fragments arranged by size: 4009 1461 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6022 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6022 NotI GC'GGCC_GC Cuts at: 3469 3469 Size: 6022 NspI r_CATG'y Cuts at: 1132 1229 3380 4925 1132 Size: 97 2151 1545 2229 Fragments arranged by size: 2229 2151 1545 97 PasI CC'CwG_GG Cuts at: 1348 2690 1348 Size: 1342 4680 PciI A'CATG_T Cuts at: 4921 4921 Size: 6022 PflMI CCAn_nnn'nTGG Cuts at: 1108 2596 1108 Size: 1488 4534 PfoI T'CCnGG_A Cuts at: 2292 2484 2589 2808 3015 3276 2292 Size: 192 105 219 207 261 5038 Fragments arranged by size: 5038 261 219 207 192 105 PmeI GTTT'AAAC Cuts at: 3326 3326 Size: 6022 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2456 2488 4091 4123 5604 5636 2456 Size: 32 1603 32 1481 32 2842 Fragments arranged by size: 2842 1603 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6022 PsiI TTA'TAA Cuts at: 3592 3592 Size: 6022 PspOMI G'GGCC_C Cuts at: 2684 3183 2684 Size: 499 5523 PstI C_TGCA'G Cuts at: 830 3374 830 Size: 2544 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6022 PvuII CAG'CTG Cuts at: 2770 2953 2770 Size: 183 5839 SacI G_AGCT'C Cuts at: 2202 2292 3249 2202 Size: 90 957 4975 Fragments arranged by size: 4975 957 90 SalI G'TCGA_C Cuts at: 1936 3364 1936 Size: 1428 4594 SbfI CC_TGCA'GG Cuts at: 3374 3374 Size: 6022 ScaI AGT'ACT Cuts at: 4270 5982 4270 Size: 1712 4310 SexAI A'CCwGG_T Cuts at: 2451 2451 Size: 6022 SfcI C'TryA_G Cuts at: 826 1045 3370 4505 5186 5377 826 Size: 219 2325 1135 681 191 1471 Fragments arranged by size: 2325 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6022 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6022 SmaI CCC'GGG Cuts at: 1872 2717 3089 3349 1872 Size: 845 372 260 4545 Fragments arranged by size: 4545 845 372 260 SmlI C'TyrA_G Cuts at: 820 1017 1943 2105 4126 5027 5289 820 Size: 197 926 162 2021 901 262 1553 Fragments arranged by size: 2021 1553 926 901 262 197 162 SnaBI TAC'GTA Cuts at: 493 3813 493 Size: 3320 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6022 SphI G_CATG'C Cuts at: 1132 3380 1132 Size: 2248 3774 SrfI GCCC'GGGC Cuts at: 2717 3089 2717 Size: 372 5650 SspI AAT'ATT Cuts at: 5 52 2023 2335 3946 5 Size: 47 1971 312 1611 2081 Fragments arranged by size: 2081 1971 1611 312 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6022 StyI C'CwwG_G Cuts at: 513 1065 2526 2829 3461 513 Size: 552 1461 303 632 3074 Fragments arranged by size: 3074 1461 632 552 303 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2207 2447 2990 3266 4049 4066 4219 Size: 1255 627 240 543 276 783 17 153 Cuts at: 4219 4404 4849 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 783 627 543 445 276 240 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4268 5980 96 Size: 274 80 33 51 1443 2291 1712 138 Fragments arranged by size: 2291 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2439 2471 5982 6014 2439 Size: 32 3511 32 2447 Fragments arranged by size: 3511 2447 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6022 XbaI T'CTAG_A Cuts at: 3358 3358 Size: 6022 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2321 2338 3004 2321 Size: 17 666 5339 Fragments arranged by size: 5339 666 17 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6022 XmaI C'CCGG_G Cuts at: 1870 2715 3087 3347 1870 Size: 845 372 260 4545 Fragments arranged by size: 4545 845 372 260 XmnI GAAnn'nnTTC Cuts at: 4151 4151 Size: 6022 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3830 276 Size: 53 83 186 936 2296 2468 Fragments arranged by size: 2468 2296 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SwaI