(Linear) (Six-base) MAPSORT of: KIEE4424.seq Check: 6716 from: 1 to: 1317 KIEE4424.orf With 182 enzymes: SgfI * March 22, 2012 17:59 .. AccI GT'mk_AC Cuts at: 0 530 1317 Size: 530 787 AfeI AGC'GCT Cuts at: 0 883 1317 Size: 883 434 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 154 1317 Size: 154 1163 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 451 483 1317 Size: 451 32 834 Fragments arranged by size: 834 451 32 AlwNI CAG_nnn'CTG Cuts at: 0 591 1317 Size: 591 726 ApaI G_GGCC'C Cuts at: 0 683 1182 1317 Size: 683 499 135 Fragments arranged by size: 683 499 135 AvaI C'yCGr_G Cuts at: 0 710 1032 1082 1317 Size: 710 322 50 235 Fragments arranged by size: 710 322 235 50 BamHI G'GATC_C Cuts at: 0 647 1317 Size: 647 670 BanI G'GyrC_C Cuts at: 0 322 790 874 1231 1317 Size: 322 468 84 357 86 Fragments arranged by size: 468 357 322 86 84 BanII G_rGCy'C Cuts at: 0 197 287 683 1182 1244 1317 Size: 197 90 396 499 62 73 Fragments arranged by size: 499 396 197 90 73 62 BbsI GAAGACnn'nnnn_ Cuts at: 0 703 1317 Size: 703 614 BclI T'GATC_A Cuts at: 0 368 1317 Size: 368 949 Bme1580I G_kGCm'C Cuts at: 0 683 1182 1317 Size: 683 499 135 Fragments arranged by size: 683 499 135 BmgBI CAC'GTC Cuts at: 0 1134 1317 Size: 1134 183 BmrI ACTGGGnnnn_n' Cuts at: 0 1176 1317 Size: 1176 141 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 522 1317 Size: 522 795 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 502 952 1294 1317 Size: 502 450 342 23 Fragments arranged by size: 502 450 342 23 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 85 1317 Size: 85 1232 BsaI GGTCTCn'nnnn_ Cuts at: 0 437 1317 Size: 437 880 BsaBI GATnn'nnATC Cuts at: 0 157 296 1317 Size: 157 139 1021 Fragments arranged by size: 1021 157 139 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 253 283 325 355 434 451 464 481 Size: 253 30 42 30 79 17 13 17 Cuts at: 481 1317 Size: 836 Fragments arranged by size: 836 253 79 42 30 30 17 17 13 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 106 130 328 1015 1090 1159 1317 Size: 106 24 198 687 75 69 158 Fragments arranged by size: 687 198 158 106 75 69 24 BseYI C'CCAG_C Cuts at: 0 761 901 1317 Size: 761 140 416 Fragments arranged by size: 761 416 140 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 790 937 1143 1300 1317 Size: 790 147 206 157 17 Fragments arranged by size: 790 206 157 147 17 BsiEI CG_ry'CG Cuts at: 0 56 1317 Size: 56 1261 BsiHKAI G_wGCw'C Cuts at: 0 101 197 287 1244 1317 Size: 101 96 90 957 73 Fragments arranged by size: 957 101 96 90 73 BsmBI CGTCTCn'nnnn_ Cuts at: 0 478 1317 Size: 478 839 Bsp1286I G_dGCh'C Cuts at: 0 101 197 287 683 1182 1244 1317 Size: 101 96 90 396 499 62 73 Fragments arranged by size: 499 396 101 96 90 73 62 BspHI T'CATG_A Cuts at: 0 161 1317 Size: 161 1156 BsrBI CCG'CTC Cuts at: 0 1031 1317 Size: 1031 286 BssSI C'ACGA_G Cuts at: 0 1249 1317 Size: 1249 68 BstXI CCAn_nnnn'nTGG Cuts at: 0 952 1317 Size: 952 365 BstYI r'GATC_y Cuts at: 0 581 647 1100 1317 Size: 581 66 453 217 Fragments arranged by size: 581 453 217 66 BtgI C'CryG_G Cuts at: 0 110 521 542 1235 1317 Size: 110 411 21 693 82 Fragments arranged by size: 693 411 110 82 21 BtsI GCAGTG_nn' Cuts at: 0 1219 1317 Size: 1219 98 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 983 1018 1317 Size: 983 35 299 Fragments arranged by size: 983 299 35 EaeI y'GGCC_r Cuts at: 0 821 1317 Size: 821 496 EarI CTCTTCn'nnn_ Cuts at: 0 160 1317 Size: 160 1157 EcoICRI GAG'CTC Cuts at: 0 195 285 1242 1317 Size: 195 90 957 75 Fragments arranged by size: 957 195 90 75 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 502 952 1294 1317 Size: 502 450 342 23 Fragments arranged by size: 502 450 342 23 EcoNI CCTnn'n_nnAGG Cuts at: 0 659 823 1317 Size: 659 164 494 Fragments arranged by size: 659 494 164 EcoO109I rG'GnC_Cy Cuts at: 0 680 1178 1317 Size: 680 498 139 Fragments arranged by size: 680 498 139 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 756 1317 Size: 756 561 FspI TGC'GCA Cuts at: 0 1225 1317 Size: 1225 92 HaeII r_GCGC'y Cuts at: 0 838 885 1317 Size: 838 47 432 Fragments arranged by size: 838 432 47 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 325 357 639 671 1317 Size: 325 32 282 32 646 Fragments arranged by size: 646 325 282 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 296 675 842 1317 Size: 296 379 167 475 Fragments arranged by size: 475 379 296 167 MscI TGG'CCA Cuts at: 0 823 1317 Size: 823 494 MspA1I CmG'CkG Cuts at: 0 765 948 1157 1317 Size: 765 183 209 160 Fragments arranged by size: 765 209 183 160 NcoI C'CATG_G Cuts at: 0 521 1317 Size: 521 796 PasI CC'CwG_GG Cuts at: 0 685 1317 Size: 685 632 PflMI CCAn_nnn'nTGG Cuts at: 0 591 1317 Size: 591 726 PfoI T'CCnGG_A Cuts at: 0 287 479 584 803 1010 1271 1317 Size: 287 192 105 219 207 261 46 Fragments arranged by size: 287 261 219 207 192 105 46 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 451 483 1317 Size: 451 32 834 Fragments arranged by size: 834 451 32 PspOMI G'GGCC_C Cuts at: 0 679 1178 1317 Size: 679 499 139 Fragments arranged by size: 679 499 139 PvuII CAG'CTG Cuts at: 0 765 948 1317 Size: 765 183 369 Fragments arranged by size: 765 369 183 SacI G_AGCT'C Cuts at: 0 197 287 1244 1317 Size: 197 90 957 73 Fragments arranged by size: 957 197 90 73 SexAI A'CCwGG_T Cuts at: 0 446 1317 Size: 446 871 SmaI CCC'GGG Cuts at: 0 712 1084 1317 Size: 712 372 233 Fragments arranged by size: 712 372 233 SmlI C'TyrA_G Cuts at: 0 100 1317 Size: 100 1217 SrfI GCCC'GGGC Cuts at: 0 712 1084 1317 Size: 712 372 233 Fragments arranged by size: 712 372 233 SspI AAT'ATT Cuts at: 0 18 330 1317 Size: 18 312 987 Fragments arranged by size: 987 312 18 StyI C'CwwG_G Cuts at: 0 521 824 1317 Size: 521 303 493 Fragments arranged by size: 521 493 303 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 202 442 985 1261 1317 Size: 202 240 543 276 56 Fragments arranged by size: 543 276 240 202 56 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 434 466 1317 Size: 434 32 851 Fragments arranged by size: 851 434 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 316 333 999 1317 Size: 316 17 666 318 Fragments arranged by size: 666 318 316 17 XmaI C'CCGG_G Cuts at: 0 710 1082 1317 Size: 710 372 235 Fragments arranged by size: 710 372 235 Enzymes that do cut: AccI AfeI AlfI AloI AlwNI ApaI AvaI BamHI BanI BanII BbsI BclI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaBI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BsrBI BssSI BstXI BstYI BtgI BtsI CspCI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I MmeI MscI MspA1I NcoI PasI PflMI PfoI PpiI PspOMI PvuII SacI SexAI SmaI SmlI SrfI SspI StyI TaqII TstI XcmI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BciVI BfrBI BglI BglII BlpI BmtI Bpu10I BsaAI BsaHI BsaWI BsiWI BsmI BspEI BspMI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EagI EciI EcoRI EcoRV FseI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MslI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PpuMI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI StuI SwaI TatI Tth111I XbaI XhoI XmnI ZraI