################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII AccI AclI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanII BbeI BbrPI BbvCI BcgI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaHI BsaI BsiHKAI BsiWI BsmFI BsmI BsoBI Bsp1286I BspDI BspLU11I BsrDI BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DraI DraII DraIII DrdI Eam1105I Eco47III EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HindIII HphI KasI KspI MaeI MfeI MluI MslI MunI NarI NciI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SpeI SphI Sse8387I SspI StuI SwaI TaqI Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### Acc65I 716 GGTACC G^GTACC AccIII 722 TCCGGA T^CCGGA AcsI 727 AAATTT [GA]^AATT[CT] AlwI 442 GATCC GGATC(4/5) AlwNI 175 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 513 CCTAAGG CC^T[ACGT]AGG ApoI 727 AAATTT [GA]^AATT[CT] Asp718I 716 GGTACC G^GTACC BanI 716 GGTACC G^G[CT][GA]CC BbsI 79 GTCTTC GAAGAC(2/6) BpuAI 79 GTCTTC GAAGAC(2/6) BsaAI 577 TACGTA [CT]AC^GT[GA] BsaBI 489 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 722 TCCGGA [AT]^CCGG[AT] BseAI 722 TCCGGA T^CCGGA BsiEI 114 CGGCCG CG[GA][CT]^CG BsmAI 233 GTCTC GTCTC(1/5) BsmBI 232 CGTCTC CGTCTC(1/5) Bsp1407I 349 TGTACA T^GTACA BspEI 722 TCCGGA T^CCGGA BspHI 708 TCATGA T^CATGA BspMI 53 ACCTGC ACCTGC(4/8) BsrBI 199 CCGCTC CCGCTC(-3/-3) BsrFI 150 GCCGGC [GA]^CCGG[CT] BsrGI 349 TGTACA T^GTACA BssHII 242 GCGCGC G^CGCGC BstYI 441 AGATCC [GA]^GATC[CT] Bsu36I 513 CCTAAGG CC^T[ACGT]AGG Cfr10I 150 GCCGGC [GA]^CCGG[CT] DsaI 65 CCGTGG C^C[GA][CT]GG EagI 114 CGGCCG C^GGCCG EarI 733 GAAGAG CTCTTC(1/4) EclXI 114 CGGCCG C^GGCCG Eco52I 114 CGGCCG C^GGCCG Eco81I 513 CCTAAGG CC^T[ACGT]AGG HaeII 29 AGCGCC [GA]GCGC^[CT] HgaI 231 GCGTC GACGC(5/10) HincII 474 GTTAAC GT[CT]^[GA]AC HindII 474 GTTAAC GT[CT]^[GA]AC HpaI 474 GTTAAC GTT^AAC KpnI 716 GGTACC GGTAC^C Ksp632I 733 GAAGAG CTCTTC(1/4) MamI 489 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 441 AGATCC [GA]^GATC[CT] MroI 722 TCCGGA T^CCGGA MspA1I 636 CAGCTG C[AC]G^C[GT]G NaeI 150 GCCGGC GCC^GGC NgoMIV 150 GCCGGC G^CCGGC NspI 482 GCATGT [GA]CATG^[CT] PleI 220 GAGTC GAGTC(4/5) PvuII 636 CAGCTG CAG^CTG RcaI 708 TCATGA T^CATGA SmlI 402 CTTGAG C^T[CT][GA]AG SnaBI 577 TACGTA TAC^GTA SspBI 349 TGTACA T^GTACA XhoII 441 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AluI 179 AGCT AG^CT 637 AGCT BalI 263 TGGCCA TGG^CCA 632 TGGCCA BciVI 544 GGATAC GTATCC(6/5) 738 GTATCC BseRI 25 GAGGAG GAGGAG(10/8) 320 GAGGAG BsgI 172 GTGCAG GTGCAG(16/14) 413 GTGCAG BstAPI 174 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 415 GCAGTGTATGC BstF5I 255 CATCC GGATG(2/0) 495 CATCC Cfr13I 158 GGGCC G^G[ACGT]CC 192 GGCCC DpnII 442 GATC ^GATC 505 GATC EcoT14I 556 CCAAGG C^C[AT][AT]GG 648 CCAAGG FauI 228 CCCGC CCCGC(4/6) 156 GCGGG FokI 255 CATCC GGATG(9/13) 495 CATCC HapII 151 CCGG C^CGG 723 CCGG HpaII 151 CCGG C^CGG 723 CCGG MaeII 578 ACGT A^CGT 673 ACGT MboI 442 GATC ^GATC 505 GATC MluNI 263 TGGCCA TGG^CCA 632 TGGCCA MscI 263 TGGCCA TGG^CCA 632 TGGCCA MseI 298 TTAA T^TAA 475 TTAA MspI 151 CCGG C^CGG 723 CCGG NdeII 442 GATC ^GATC 505 GATC NlaIV 140 GGCTCC GG[ACGT]^[ACGT]CC 716 GGTACC PstI 55 CTGCAG CTGCA^G 584 CTGCAG Sau3AI 442 GATC ^GATC 505 GATC Sau96I 158 GGGCC G^G[ACGT]CC 192 GGCCC SfcI 55 CTGCAG C^T[GA][CT]AG 584 CTGCAG StyI 556 CCAAGG C^C[AT][AT]GG 648 CCAAGG TfiI 90 GAATC G^A[AT]TC 519 GAATC Tru9I 298 TTAA T^TAA 475 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AccII AciI AfaI BbvI BsaJI BsiYI BslI BsrI BssKI BstNI BstUI Cac8I CfoI DdeI EaeI EcoRII Fnu4HI HaeIII HhaI HinP1I HinfI ItaI MaeIII MboII MnlI MvaI MvnI MwoI NlaIII RsaI ScrFI SfaNI TseI Tsp509I TspRI