(Circular) (Six-base) MAPSORT of: pFN21AE5221.seq Check: 5223 from: 1 to: 5482 pFN21AE5221 5482 bp With 182 enzymes: SgfI * January 30, 2014 17:15 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3292 278 Size: 53 83 186 936 1756 2468 Fragments arranged by size: 2468 1756 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2825 1937 Size: 888 4594 Acc65I G'GTAC_C Cuts at: 2721 2803 2721 Size: 82 5400 AclI AA'CG_TT Cuts at: 1563 3609 3982 1563 Size: 2046 373 3063 Fragments arranged by size: 3063 2046 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3545 4929 5197 1632 Size: 1913 1384 268 1917 Fragments arranged by size: 1917 1913 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5285 AflIII A'CryG_T Cuts at: 1203 4381 5358 1203 Size: 3178 977 1327 Fragments arranged by size: 3178 1327 977 AgeI A'CCGG_T Cuts at: 4313 4313 Size: 5482 AhdI GACnn_n'nnGTC Cuts at: 4211 4211 Size: 5482 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5482 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5482 AlwNI CAG_nnn'CTG Cuts at: 2185 3225 4797 2185 Size: 1040 1572 2870 Fragments arranged by size: 2870 1572 1040 ApaLI G'TGCA_C Cuts at: 3539 4695 3539 Size: 1156 4326 ApoI r'AATT_y Cuts at: 1493 2732 2791 3017 3169 5278 1493 Size: 1239 59 226 152 2109 1697 Fragments arranged by size: 2109 1697 1239 226 152 59 AseI AT'TA_AT Cuts at: 160 1032 4036 160 Size: 872 3004 1606 Fragments arranged by size: 3004 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5482 AvaI C'yCGr_G Cuts at: 1870 1943 2807 1870 Size: 73 864 4545 Fragments arranged by size: 4545 864 73 BamHI G'GATC_C Cuts at: 2812 3179 2812 Size: 367 5115 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2721 2803 4258 618 Size: 325 217 627 934 82 1455 1842 Fragments arranged by size: 1842 1455 934 627 325 217 82 BanII G_rGCy'C Cuts at: 1391 2801 1391 Size: 1410 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2077 928 Size: 971 178 4333 Fragments arranged by size: 4333 971 178 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3673 3707 1903 Size: 34 1736 34 3678 Fragments arranged by size: 3678 1736 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2543 2754 3374 4584 2543 Size: 211 620 1210 3441 Fragments arranged by size: 3441 1210 620 211 BclI T'GATC_A Cuts at: 1548 1548 Size: 5482 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4093 136 Size: 107 122 71 631 605 2421 1525 Fragments arranged by size: 2421 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5477 5477 Size: 5482 BlpI GC'TnA_GC Cuts at: 2899 2899 Size: 5482 Bme1580I G_kGCm'C Cuts at: 1790 3543 4699 1790 Size: 1753 1156 2573 Fragments arranged by size: 2573 1753 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5482 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2446 3213 4171 Size: 416 53 83 399 24 1029 767 958 Cuts at: 4171 442 Size: 1753 Fragments arranged by size: 1753 1029 958 767 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5482 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2206 2206 Size: 5482 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4142 1239 Size: 2903 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2428 3607 4472 4770 2428 Size: 1179 865 298 3140 Fragments arranged by size: 3140 1179 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4145 882 Size: 617 2646 2219 Fragments arranged by size: 2646 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2584 3273 493 Size: 711 1380 689 2702 Fragments arranged by size: 2702 1380 711 689 BsaBI GATnn'nnATC Cuts at: 2498 5476 2498 Size: 2978 2504 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3289 3671 4306 275 Size: 53 83 186 936 1756 382 635 1451 Fragments arranged by size: 1756 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2727 3915 4313 4587 4734 5145 2727 Size: 1188 398 274 147 411 3064 Fragments arranged by size: 3064 1188 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5257 Fragments arranged by size: 5257 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2045 2340 1186 Size: 859 295 4328 Fragments arranged by size: 4328 859 295 BseYI C'CCAG_C Cuts at: 2135 4685 5181 2135 Size: 2550 496 2436 Fragments arranged by size: 2550 2436 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2198 2439 1163 Size: 573 462 241 4206 Fragments arranged by size: 4206 573 462 241 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2122 2932 3693 4721 1540 Size: 275 186 121 810 761 1028 2301 Fragments arranged by size: 2301 1028 810 761 275 186 121 BsiHKAI G_wGCw'C Cuts at: 3543 3628 4699 3543 Size: 85 1071 4326 Fragments arranged by size: 4326 1071 85 BsmI GAATG_Cn' Cuts at: 2993 3086 2993 Size: 93 5389 BsmBI CGTCTCn'nnnn_ Cuts at: 2243 2243 Size: 5482 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2801 3543 3628 4699 1391 Size: 399 1011 742 85 1071 2174 Fragments arranged by size: 2174 1071 1011 742 399 85 BspEI T'CCGG_A Cuts at: 2727 5145 2727 Size: 2418 3064 BspHI T'CATG_A Cuts at: 2713 3371 5101 2713 Size: 658 1730 3094 Fragments arranged by size: 3094 1730 658 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2067 2837 844 Size: 1223 770 3489 Fragments arranged by size: 3489 1223 770 BsrBI CCG'CTC Cuts at: 1394 1823 2206 3369 1394 Size: 429 383 1163 3507 Fragments arranged by size: 3507 1163 429 383 BsrDI GCAATG_nn' Cuts at: 65 1248 3977 4151 65 Size: 1183 2729 174 1396 Fragments arranged by size: 2729 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2155 4126 4313 1700 Size: 110 345 1971 187 2869 Fragments arranged by size: 2869 1971 345 187 110 BsrGI T'GTAC_A Cuts at: 96 2354 96 Size: 2258 3224 BssHII G'CGCG_C Cuts at: 1925 2247 1925 Size: 322 5160 BssSI C'ACGA_G Cuts at: 3542 4554 3542 Size: 1012 4470 BstAPI GCAn_nnn'nTGC Cuts at: 794 2185 2426 794 Size: 1391 241 3850 Fragments arranged by size: 3850 1391 241 BstBI TT'CG_AA Cuts at: 4286 4286 Size: 5482 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5482 BstYI r'GATC_y Cuts at: 1972 2446 2812 3179 3564 3581 5033 5119 5131 Size: 474 366 367 385 17 1452 86 12 Cuts at: 5131 5477 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 474 385 367 366 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2519 2519 Size: 5482 BtgI C'CryG_G Cuts at: 513 1065 2004 2070 513 Size: 552 939 66 3925 Fragments arranged by size: 3925 939 552 66 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4743 Fragments arranged by size: 4743 683 56 BtsI GCAGTG_nn' Cuts at: 2069 2427 2998 3792 3812 2069 Size: 358 571 794 20 3739 Fragments arranged by size: 3739 794 571 358 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5482 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5447 DraI TTT'AAA Cuts at: 2786 2786 Size: 5482 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4489 809 Size: 507 3173 1802 Fragments arranged by size: 3173 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2043 2119 2268 Size: 54 1425 50 275 18 213 76 149 Cuts at: 2268 2637 2929 3818 5447 8 Size: 369 292 889 1629 43 Fragments arranged by size: 1629 1425 889 369 292 275 213 149 76 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2119 2929 1537 Size: 275 307 810 4090 Fragments arranged by size: 4090 810 307 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2733 3412 1353 Size: 1380 679 3423 Fragments arranged by size: 3423 1380 679 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2042 2218 4069 4443 4589 387 Size: 1064 138 453 176 1851 374 146 1280 Fragments arranged by size: 1851 1280 1064 453 374 176 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3545 4142 4929 5197 1239 Size: 393 1913 597 787 268 1524 Fragments arranged by size: 1913 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 2791 3169 2791 Size: 378 5104 FspI TGC'GCA Cuts at: 3988 3988 Size: 5482 HaeII r_GCGC'y Cuts at: 1678 2038 4629 1678 Size: 360 2591 2531 Fragments arranged by size: 2591 2531 360 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4123 4155 4197 4229 1373 Size: 32 135 32 2551 32 42 32 2626 Fragments arranged by size: 2626 2551 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2481 2826 3072 669 Size: 988 281 543 345 246 3079 Fragments arranged by size: 3079 988 543 345 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5482 HpaI GTT'AAC Cuts at: 2481 3072 2481 Size: 591 4891 KpnI G_GTAC'C Cuts at: 2725 2807 2725 Size: 82 5400 MfeI C'AATT_G Cuts at: 3081 3081 Size: 5482 MluI A'CGCG_T Cuts at: 5358 5358 Size: 5482 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4596 4780 1305 Size: 3291 184 2007 Fragments arranged by size: 3291 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2270 2639 10 Size: 54 1425 343 438 369 2853 Fragments arranged by size: 2853 1425 438 369 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3440 3799 3958 518 Size: 648 2274 359 159 2042 Fragments arranged by size: 2274 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2643 2898 3575 4723 4968 1606 Size: 1037 255 677 1148 245 2120 Fragments arranged by size: 2120 1148 1037 677 255 245 NaeI GCC'GGC Cuts at: 2157 2157 Size: 5482 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3991 Fragments arranged by size: 3991 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5482 NgoMIV G'CCGG_C Cuts at: 2155 2155 Size: 5482 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5482 NotI GC'GGCC_GC Cuts at: 2929 2929 Size: 5482 NspI r_CATG'y Cuts at: 1132 1229 2491 2840 4385 1132 Size: 97 1262 349 1545 2229 Fragments arranged by size: 2229 1545 1262 349 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5482 PciI A'CATG_T Cuts at: 4381 4381 Size: 5482 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5482 PmeI GTTT'AAAC Cuts at: 2786 2786 Size: 5482 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3551 3583 5064 5096 3551 Size: 32 1481 32 3937 Fragments arranged by size: 3937 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5482 PsiI TTA'TAA Cuts at: 2765 3052 2765 Size: 287 5195 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2099 2131 2735 2767 2099 Size: 32 604 32 4814 Fragments arranged by size: 4814 604 32 32 PstI C_TGCA'G Cuts at: 830 2064 2593 2834 830 Size: 1234 529 241 3478 Fragments arranged by size: 3478 1234 529 241 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5482 PvuII CAG'CTG Cuts at: 2643 2643 Size: 5482 SalI G'TCGA_C Cuts at: 1936 2824 1936 Size: 888 4594 SbfI CC_TGCA'GG Cuts at: 2834 2834 Size: 5482 ScaI AGT'ACT Cuts at: 3730 5442 3730 Size: 1712 3770 SfcI C'TryA_G Cuts at: 826 1045 2060 2589 2830 3965 4646 4837 826 Size: 219 1015 529 241 1135 681 191 1471 Fragments arranged by size: 1471 1135 1015 681 529 241 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5482 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5482 SmaI CCC'GGG Cuts at: 1872 2809 1872 Size: 937 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2407 3586 4487 4749 820 Size: 197 926 464 1179 901 262 1553 Fragments arranged by size: 1553 1179 926 901 464 262 197 SnaBI TAC'GTA Cuts at: 493 2584 3273 493 Size: 2091 689 2702 Fragments arranged by size: 2702 2091 689 SpeI A'CTAG_T Cuts at: 152 152 Size: 5482 SphI G_CATG'C Cuts at: 1132 2840 1132 Size: 1708 3774 SspI AAT'ATT Cuts at: 5 52 3406 5 Size: 47 3354 2081 Fragments arranged by size: 3354 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5482 StyI C'CwwG_G Cuts at: 513 1065 2004 2561 2653 2921 513 Size: 552 939 557 92 268 3074 Fragments arranged by size: 3074 939 557 552 268 92 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2161 3509 3526 3679 3864 4309 325 Size: 1255 581 1348 17 153 185 445 1498 Fragments arranged by size: 1498 1348 1255 581 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2354 3728 5440 Size: 274 80 33 51 1443 377 1374 1712 Cuts at: 5440 96 Size: 138 Fragments arranged by size: 1712 1443 1374 377 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5442 5474 5442 Size: 32 5450 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5482 XbaI T'CTAG_A Cuts at: 2818 2818 Size: 5482 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5482 XmaI C'CCGG_G Cuts at: 1870 2807 1870 Size: 937 4545 XmnI GAAnn'nnTTC Cuts at: 3611 3611 Size: 5482 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3290 276 Size: 53 83 186 936 1756 2468 Fragments arranged by size: 2468 1756 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI