(Linear) (Six-base) MAPSORT of: KIEE5221.seq Check: 6657 from: 1 to: 777 KIEE5221.orf With 182 enzymes: SgfI * January 29, 2014 11:51 .. Acc65I G'GTAC_C Cuts at: 0 716 777 Size: 716 61 AlwNI CAG_nnn'CTG Cuts at: 0 180 777 Size: 180 597 ApoI r'AATT_y Cuts at: 0 727 777 Size: 727 50 BanI G'GyrC_C Cuts at: 0 716 777 Size: 716 61 BbsI GAAGACnn'nnnn_ Cuts at: 0 72 777 Size: 72 705 BciVI GTATCCnnnnn_n' Cuts at: 0 538 749 777 Size: 538 211 28 Fragments arranged by size: 538 211 28 BmrI ACTGGGnnnn_n' Cuts at: 0 441 777 Size: 441 336 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 201 777 Size: 201 576 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 423 777 Size: 423 354 BsaAI yAC'GTr Cuts at: 0 579 777 Size: 579 198 BsaBI GATnn'nnATC Cuts at: 0 493 777 Size: 493 284 BsaWI w'CCGG_w Cuts at: 0 722 777 Size: 722 55 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 40 335 777 Size: 40 295 442 Fragments arranged by size: 442 295 40 BseYI C'CCAG_C Cuts at: 0 130 777 Size: 130 647 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 193 434 777 Size: 193 241 343 Fragments arranged by size: 343 241 193 BsiEI CG_ry'CG Cuts at: 0 117 777 Size: 117 660 BsmBI CGTCTCn'nnnn_ Cuts at: 0 238 777 Size: 238 539 BspEI T'CCGG_A Cuts at: 0 722 777 Size: 722 55 BspHI T'CATG_A Cuts at: 0 708 777 Size: 708 69 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 62 777 Size: 62 715 BsrBI CCG'CTC Cuts at: 0 201 777 Size: 201 576 BsrFI r'CCGG_y Cuts at: 0 150 777 Size: 150 627 BsrGI T'GTAC_A Cuts at: 0 349 777 Size: 349 428 BssHII G'CGCG_C Cuts at: 0 242 777 Size: 242 535 BstAPI GCAn_nnn'nTGC Cuts at: 0 180 421 777 Size: 180 241 356 Fragments arranged by size: 356 241 180 BstYI r'GATC_y Cuts at: 0 441 777 Size: 441 336 Bsu36I CC'TnA_GG Cuts at: 0 514 777 Size: 514 263 BtgI C'CryG_G Cuts at: 0 65 777 Size: 65 712 BtsI GCAGTG_nn' Cuts at: 0 64 422 777 Size: 64 358 355 Fragments arranged by size: 358 355 64 EaeI y'GGCC_r Cuts at: 0 38 114 263 632 777 Size: 38 76 149 369 145 Fragments arranged by size: 369 149 145 76 38 EagI C'GGCC_G Cuts at: 0 114 777 Size: 114 663 EarI CTCTTCn'nnn_ Cuts at: 0 728 777 Size: 728 49 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 37 213 777 Size: 37 176 564 Fragments arranged by size: 564 176 37 HaeII r_GCGC'y Cuts at: 0 33 777 Size: 33 744 HincII GTy'rAC Cuts at: 0 476 777 Size: 476 301 HpaI GTT'AAC Cuts at: 0 476 777 Size: 476 301 KpnI G_GTAC'C Cuts at: 0 720 777 Size: 720 57 MscI TGG'CCA Cuts at: 0 265 634 777 Size: 265 369 143 Fragments arranged by size: 369 265 143 MspA1I CmG'CkG Cuts at: 0 638 777 Size: 638 139 NaeI GCC'GGC Cuts at: 0 152 777 Size: 152 625 NgoMIV G'CCGG_C Cuts at: 0 150 777 Size: 150 627 NspI r_CATG'y Cuts at: 0 486 777 Size: 486 291 PsiI TTA'TAA Cuts at: 0 760 777 Size: 760 17 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 94 126 730 762 777 Size: 94 32 604 32 15 Fragments arranged by size: 604 94 32 32 15 PstI C_TGCA'G Cuts at: 0 59 588 777 Size: 59 529 189 Fragments arranged by size: 529 189 59 PvuII CAG'CTG Cuts at: 0 638 777 Size: 638 139 SfcI C'TryA_G Cuts at: 0 55 584 777 Size: 55 529 193 Fragments arranged by size: 529 193 55 SmlI C'TyrA_G Cuts at: 0 402 777 Size: 402 375 SnaBI TAC'GTA Cuts at: 0 579 777 Size: 579 198 StyI C'CwwG_G Cuts at: 0 556 648 777 Size: 556 92 129 Fragments arranged by size: 556 129 92 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 156 777 Size: 156 621 TatI w'GTAC_w Cuts at: 0 349 777 Size: 349 428 Enzymes that do cut: Acc65I AlwNI ApoI BanI BbsI BciVI BmrI BplI BpuEI BsaAI BsaBI BsaWI BseRI BseYI BsgI BsiEI BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstYI Bsu36I BtgI BtsI EaeI EagI EarI EciI HaeII HincII HpaI KpnI MscI MspA1I NaeI NgoMIV NspI PsiI PsrI PstI PvuII SfcI SmlI SnaBI StyI TaqII TatI Enzymes that do not cut: AarI AatII AccI AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanII BbeI BbvCI BcgI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI BpmI Bpu10I BsaI BsaHI BsaXI BsiHKAI BsiWI BsmI Bsp1286I BsrDI BssSI BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI Hin4I HindIII KasI MfeI MluI MmeI MslI NarI NcoI NdeI NheI NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI