Restriction map for
pFN21AE1575
(Circular) (Six-base) MAPSORT of: pFN21AE1575.seq Check: 504 from: 1 to: 5800
pFN21AE1575 5800 bp
With 182 enzymes: SgfI *
August 16, 2010 14:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2858 2858
Size: 5800
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3610 278
Size: 53 83 186 936 2074 2468
Fragments arranged by size:
2468 2074 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3143 1937
Size: 1206 4594
Acc65I G'GTAC_C
Cuts at: 2412 3121 2412
Size: 709 5091
AclI AA'CG_TT
Cuts at: 1563 3927 4300 1563
Size: 2364 373 3063
Fragments arranged by size:
3063 2364 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3863 5247 5515 1632
Size: 2231 1384 268 1917
Fragments arranged by size:
2231 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5603
AflIII A'CryG_T
Cuts at: 1203 4699 5676 1203
Size: 3496 977 1327
Fragments arranged by size:
3496 1327 977
AgeI A'CCGG_T
Cuts at: 4631 4631
Size: 5800
AhdI GACnn_n'nnGTC
Cuts at: 4529 4529
Size: 5800
AleI CACnn'nnGTG
Cuts at: 1166 2108 1166
Size: 942 4858
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5800
AlwNI CAG_nnn'CTG
Cuts at: 3543 5115 3543
Size: 1572 4228
ApaI G_GGCC'C
Cuts at: 2053 2692 2053
Size: 639 5161
ApaLI G'TGCA_C
Cuts at: 3857 5013 3857
Size: 1156 4644
ApoI r'AATT_y
Cuts at: 1493 3109 3335 3487 5596 1493
Size: 1616 226 152 2109 1697
Fragments arranged by size:
2109 1697 1616 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4354 160
Size: 872 3322 1606
Fragments arranged by size:
3322 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
AvaI C'yCGr_G
Cuts at: 1870 1943 2044 3125 1870
Size: 73 101 1081 4545
Fragments arranged by size:
4545 1081 101 73
BamHI G'GATC_C
Cuts at: 3130 3497 3130
Size: 367 5433
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2017 2221 2412 2821 3121
Size: 325 217 627 230 204 191 409 300
Cuts at: 3121 4576 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 409 325 300 230 217
204 191
BanII G_rGCy'C
Cuts at: 1391 2053 2081 2136 2692 2766 3014 3071 3119
Size: 662 28 55 556 74 248 57 48
Cuts at: 3119 1391
Size: 4072
Fragments arranged by size:
4072 662 556 248 74 57 55 48
28
BbeI G_GCGC'C
Cuts at: 2021 2225 2021
Size: 204 5596
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2898 928
Size: 971 999 3830
Fragments arranged by size:
3830 999 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3991 4025 1903
Size: 34 2054 34 3678
Fragments arranged by size:
3678 2054 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3692 4902 3692
Size: 1210 4590
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5800
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2015 2395 2531
Size: 107 122 71 631 605 343 380 136
Cuts at: 2531 4411 136
Size: 1880 1525
Fragments arranged by size:
1880 1525 631 605 380 343 136 122
107 71
BglII A'GATC_T
Cuts at: 5795 5795
Size: 5800
BlpI GC'TnA_GC
Cuts at: 3217 3217
Size: 5800
Bme1580I G_kGCm'C
Cuts at: 1790 2053 2692 3064 3861 5017 1790
Size: 263 639 372 797 1156 2573
Fragments arranged by size:
2573 1156 797 639 372 263
BmgBI CAC'GTC
Cuts at: 1318 2383 1318
Size: 1065 4735
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3531 4489 442
Size: 416 53 83 399 24 2114 958 1753
Fragments arranged by size:
2114 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5800
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3054 3054
Size: 5800
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2576 2612 2994 4460 1239
Size: 1337 36 382 1466 2579
Fragments arranged by size:
2579 1466 1337 382 36
Bpu10I CC'TnA_GC
Cuts at: 2693 2693
Size: 5800
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3925 4790 5088 3925
Size: 865 298 4637
Fragments arranged by size:
4637 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2765 4463 882
Size: 617 1266 1698 2219
Fragments arranged by size:
2219 1698 1266 617
BsaAI yAC'GTr
Cuts at: 493 1204 2158 3591 493
Size: 711 954 1433 2702
Fragments arranged by size:
2702 1433 954 711
BsaBI GATnn'nnATC
Cuts at: 5794 5794
Size: 5800
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2018 2193 2222 2625
Size: 53 83 186 936 485 175 29 403
Cuts at: 2625 2810 3607 3989 4624 275
Size: 185 797 382 635 1451
Fragments arranged by size:
1451 936 797 635 485 403 382 186
185 175 83 53 29
BsaWI w'CCGG_w
Cuts at: 2259 4233 4631 4905 5052 5463 2259
Size: 1974 398 274 147 411 2596
Fragments arranged by size:
2596 1974 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2549 2579 2967 2997 1178
Size: 30 165 30 1146 30 388 30 3981
Fragments arranged by size:
3981 1146 388 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3016 1186
Size: 1830 3970
BseYI C'CCAG_C
Cuts at: 2505 2685 2910 5003 5499 2505
Size: 180 225 2093 496 2806
Fragments arranged by size:
2806 2093 496 225 180
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2879 1163
Size: 573 1143 4084
Fragments arranged by size:
4084 1143 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2125 3250 4011 5039 1540
Size: 275 186 124 1125 761 1028 2301
Fragments arranged by size:
2301 1125 1028 761 275 186 124
BsiHKAI G_wGCw'C
Cuts at: 2766 3014 3861 3946 5017 2766
Size: 248 847 85 1071 3549
Fragments arranged by size:
3549 1071 847 248 85
BsmI GAATG_Cn'
Cuts at: 3311 3404 3311
Size: 93 5707
BsmBI CGTCTCn'nnnn_
Cuts at: 2513 2600 2513
Size: 87 5713
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2053 2081 2136 2692 2766 3014 3064
Size: 399 263 28 55 556 74 248 50
Cuts at: 3064 3071 3119 3861 3946 5017 1391
Size: 7 48 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 556 399 263 248 85
74 55 50 48 28 7
BspEI T'CCGG_A
Cuts at: 5463 5463
Size: 5800
BspHI T'CATG_A
Cuts at: 3689 5419 3689
Size: 1730 4070
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2472 2858 3155 844
Size: 1628 386 297 3489
Fragments arranged by size:
3489 1628 386 297
BsrBI CCG'CTC
Cuts at: 1394 1823 3687 1394
Size: 429 1864 3507
Fragments arranged by size:
3507 1864 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2937 4295 4469 65
Size: 1183 1689 1358 174 1396
Fragments arranged by size:
1689 1396 1358 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2562 4444 4631 1700
Size: 110 752 1882 187 2869
Fragments arranged by size:
2869 1882 752 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5800
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5800
BssSI C'ACGA_G
Cuts at: 2765 3860 4872 2765
Size: 1095 1012 3693
Fragments arranged by size:
3693 1095 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2176 794
Size: 1382 4418
BstBI TT'CG_AA
Cuts at: 4604 4604
Size: 5800
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5800
BstYI r'GATC_y
Cuts at: 1972 3130 3497 3882 3899 5351 5437 5449 5795
Size: 1158 367 385 17 1452 86 12 346
Cuts at: 5795 1972
Size: 1977
Fragments arranged by size:
1977 1452 1158 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2678 2678
Size: 5800
BtgI C'CryG_G
Cuts at: 513 1065 2004 2125 2148 2216 2225 2407 2478
Size: 552 939 121 23 68 9 182 71
Cuts at: 2478 513
Size: 3835
Fragments arranged by size:
3835 939 552 182 121 71 68 23
9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2128 2431 487
Size: 683 56 902 303 3856
Fragments arranged by size:
3856 902 683 303 56
BtsI GCAGTG_nn'
Cuts at: 2546 3058 3316 4110 4130 2546
Size: 512 258 794 20 4216
Fragments arranged by size:
4216 794 512 258 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5800
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5765
DraI TTT'AAA
Cuts at: 3104 3104
Size: 5800
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4807 809
Size: 507 3491 1802
Fragments arranged by size:
3491 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2122 3247
Size: 54 1425 50 275 18 177 115 1125
Cuts at: 3247 4136 5765 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1125 889 275 177 115 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2122 3247 1537
Size: 275 310 1125 4090
Fragments arranged by size:
4090 1125 310 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3002 3730 1353
Size: 1649 728 3423
Fragments arranged by size:
3423 1649 728
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2498 2549 4387 4761 4907 387
Size: 1064 138 909 51 1838 374 146 1280
Fragments arranged by size:
1838 1280 1064 909 374 146 138 51
EcoICRI GAG'CTC
Cuts at: 2764 3012 2764
Size: 248 5552
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2576 2612 2994 3863 4460 5247 5515
Size: 393 944 36 382 869 597 787 268
Cuts at: 5515 1239
Size: 1524
Fragments arranged by size:
1524 944 869 787 597 393 382 268
36
EcoNI CCTnn'n_nnAGG
Cuts at: 2456 2456
Size: 5800
EcoO109I rG'GnC_Cy
Cuts at: 2049 2050 2461 2689 2793 2049
Size: 1 411 228 104 5056
Fragments arranged by size:
5056 411 228 104 1
EcoRI G'AATT_C
Cuts at: 3109 3487 3109
Size: 378 5422
FspI TGC'GCA
Cuts at: 4306 4306
Size: 5800
HaeII r_GCGC'y
Cuts at: 1678 2021 2225 2401 4947 1678
Size: 343 204 176 2546 2531
Fragments arranged by size:
2546 2531 343 204 176
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2453 2485 2952 2967 2984
Size: 32 135 32 881 32 467 15 17
Cuts at: 2984 2999 4441 4473 4515 4547 1373
Size: 15 1442 32 42 32 2626
Fragments arranged by size:
2626 1442 881 467 135 42 32 32
32 32 32 17 15 15
HincII GTy'rAC
Cuts at: 669 1657 1938 3144 3390 669
Size: 988 281 1206 246 3079
Fragments arranged by size:
3079 1206 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5800
HpaI GTT'AAC
Cuts at: 3390 3390
Size: 5800
KasI G'GCGC_C
Cuts at: 2017 2221 2017
Size: 204 5596
KpnI G_GTAC'C
Cuts at: 2416 3125 2416
Size: 709 5091
MfeI C'AATT_G
Cuts at: 3399 3399
Size: 5800
MluI A'CGCG_T
Cuts at: 5676 5676
Size: 5800
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4914 5098 1305
Size: 3609 184 2007
Fragments arranged by size:
3609 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3978
Fragments arranged by size:
3978 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2108 2147 3758 4117 4276 518
Size: 648 942 39 1611 359 159 2042
Fragments arranged by size:
2042 1611 942 648 359 159 39
MspA1I CmG'CkG
Cuts at: 1606 2127 2218 2409 2480 2731 3216 3893 5041
Size: 521 91 191 71 251 485 677 1148
Cuts at: 5041 5286 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 521 485 251 245 191
91 71
NarI GG'CG_CC
Cuts at: 2018 2222 2018
Size: 204 5596
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4309
Fragments arranged by size:
4309 939 552
NdeI CA'TA_TG
Cuts at: 387 2640 387
Size: 2253 3547
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5800
NotI GC'GGCC_GC
Cuts at: 3247 3247
Size: 5800
NspI r_CATG'y
Cuts at: 1132 1229 3158 4703 1132
Size: 97 1929 1545 2229
Fragments arranged by size:
2229 1929 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3064 1348
Size: 1716 4084
PciI A'CATG_T
Cuts at: 4699 4699
Size: 5800
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2209 1108
Size: 1101 4699
PmeI GTTT'AAAC
Cuts at: 3104 3104
Size: 5800
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3869 3901 5382 5414 3869
Size: 32 1481 32 4255
Fragments arranged by size:
4255 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2461 2793 2461
Size: 332 5468
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5800
PsiI TTA'TAA
Cuts at: 3370 3370
Size: 5800
PspOMI G'GGCC_C
Cuts at: 2049 2688 2049
Size: 639 5161
PstI C_TGCA'G
Cuts at: 830 2616 2785 3152 830
Size: 1786 169 367 3478
Fragments arranged by size:
3478 1786 367 169
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5800
SacI G_AGCT'C
Cuts at: 2766 3014 2766
Size: 248 5552
SacII CC_GC'GG
Cuts at: 2128 2219 2410 2481 2128
Size: 91 191 71 5447
Fragments arranged by size:
5447 191 91 71
SalI G'TCGA_C
Cuts at: 1936 3142 1936
Size: 1206 4594
SapI GCTCTTCn'nnn_
Cuts at: 3002 3002
Size: 5800
SbfI CC_TGCA'GG
Cuts at: 2785 3152 2785
Size: 367 5433
ScaI AGT'ACT
Cuts at: 4048 5760 4048
Size: 1712 4088
SfcI C'TryA_G
Cuts at: 826 1045 2612 2781 3148 4283 4964 5155 826
Size: 219 1567 169 367 1135 681 191 1471
Fragments arranged by size:
1567 1471 1135 681 367 219 191 169
SfoI GGC'GCC
Cuts at: 2019 2223 2019
Size: 204 5596
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5800
SmaI CCC'GGG
Cuts at: 1872 3127 1872
Size: 1255 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3904 4805 5067 820
Size: 197 926 1961 901 262 1553
Fragments arranged by size:
1961 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3591 493
Size: 3098 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5800
SphI G_CATG'C
Cuts at: 1132 3158 1132
Size: 2026 3774
SspI AAT'ATT
Cuts at: 5 52 3724 5
Size: 47 3672 2081
Fragments arranged by size:
3672 2081 47
StuI AGG'CCT
Cuts at: 1517 2779 1517
Size: 1262 4538
StyI C'CwwG_G
Cuts at: 513 1065 2004 3239 513
Size: 552 939 1235 3074
Fragments arranged by size:
3074 1235 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2313 2519 3827 3844 3997 4182 4627
Size: 1255 733 206 1308 17 153 185 445
Cuts at: 4627 325
Size: 1498
Fragments arranged by size:
1498 1308 1255 733 445 206 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4046 5758 96
Size: 274 80 33 51 1443 2069 1712 138
Fragments arranged by size:
2069 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2549 2581 5760 5792 2549
Size: 32 3179 32 2557
Fragments arranged by size:
3179 2557 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5800
XbaI T'CTAG_A
Cuts at: 3136 3136
Size: 5800
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2369 2926 2011
Size: 358 557 4885
Fragments arranged by size:
4885 557 358
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5800
XmaI C'CCGG_G
Cuts at: 1870 3125 1870
Size: 1255 4545
XmnI GAAnn'nnTTC
Cuts at: 3929 3929
Size: 5800
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3608 276
Size: 53 83 186 936 2074 2468
Fragments arranged by size:
2468 2074 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI
BstXI BstZ17I DraIII EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI
PvuII RsrII SanDI SexAI SfiI SrfI SwaI