(Linear) (Six-base) MAPSORT of: KIEE1575.seq Check: 8570 from: 1 to: 1095 KIEE1575.orf With 182 enzymes: SgfI * July 15, 2010 18:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 853 1095 Size: 853 242 Acc65I G'GTAC_C Cuts at: 0 407 1095 Size: 407 688 AleI CACnn'nnGTG Cuts at: 0 103 1095 Size: 103 992 ApaI G_GGCC'C Cuts at: 0 48 687 1095 Size: 48 639 408 Fragments arranged by size: 639 408 48 AvaI C'yCGr_G Cuts at: 0 39 1095 Size: 39 1056 BanI G'GyrC_C Cuts at: 0 12 216 407 816 1095 Size: 12 204 191 409 279 Fragments arranged by size: 409 279 204 191 12 BanII G_rGCy'C Cuts at: 0 48 76 131 687 761 1009 1066 1095 Size: 48 28 55 556 74 248 57 29 Fragments arranged by size: 556 248 74 57 55 48 29 28 BbeI G_GCGC'C Cuts at: 0 16 220 1095 Size: 16 204 875 Fragments arranged by size: 875 204 16 BbsI GAAGACnn'nnnn_ Cuts at: 0 893 1095 Size: 893 202 BglI GCCn_nnn'nGGC Cuts at: 0 10 390 526 1095 Size: 10 380 136 569 Fragments arranged by size: 569 380 136 10 Bme1580I G_kGCm'C Cuts at: 0 48 687 1059 1095 Size: 48 639 372 36 Fragments arranged by size: 639 372 48 36 BmgBI CAC'GTC Cuts at: 0 378 1095 Size: 378 717 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1049 1095 Size: 1049 46 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 571 607 989 1095 Size: 571 36 382 106 Fragments arranged by size: 571 382 106 36 Bpu10I CC'TnA_GC Cuts at: 0 688 1095 Size: 688 407 BsaI GGTCTCn'nnnn_ Cuts at: 0 760 1095 Size: 760 335 BsaAI yAC'GTr Cuts at: 0 153 1095 Size: 153 942 BsaHI Gr'CG_yC Cuts at: 0 13 188 217 620 805 1095 Size: 13 175 29 403 185 290 Fragments arranged by size: 403 290 185 175 29 13 BsaWI w'CCGG_w Cuts at: 0 254 1095 Size: 254 841 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 544 574 962 992 1095 Size: 544 30 388 30 103 Fragments arranged by size: 544 388 103 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1011 1095 Size: 1011 84 BseYI C'CCAG_C Cuts at: 0 500 680 905 1095 Size: 500 180 225 190 Fragments arranged by size: 500 225 190 180 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 874 1095 Size: 874 221 BsiEI CG_ry'CG Cuts at: 0 120 1095 Size: 120 975 BsiHKAI G_wGCw'C Cuts at: 0 761 1009 1095 Size: 761 248 86 Fragments arranged by size: 761 248 86 BsmBI CGTCTCn'nnnn_ Cuts at: 0 508 595 1095 Size: 508 87 500 Fragments arranged by size: 508 500 87 Bsp1286I G_dGCh'C Cuts at: 0 48 76 131 687 761 1009 1059 1066 Size: 48 28 55 556 74 248 50 7 Cuts at: 1066 1095 Size: 29 Fragments arranged by size: 556 248 74 55 50 48 29 28 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 467 853 1095 Size: 467 386 242 Fragments arranged by size: 467 386 242 BsrDI GCAATG_nn' Cuts at: 0 932 1095 Size: 932 163 BsrFI r'CCGG_y Cuts at: 0 557 1095 Size: 557 538 BssSI C'ACGA_G Cuts at: 0 760 1095 Size: 760 335 BstAPI GCAn_nnn'nTGC Cuts at: 0 171 1095 Size: 171 924 Bsu36I CC'TnA_GG Cuts at: 0 673 1095 Size: 673 422 BtgI C'CryG_G Cuts at: 0 120 143 211 220 402 473 1095 Size: 120 23 68 9 182 71 622 Fragments arranged by size: 622 182 120 71 68 23 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 123 426 1095 Size: 123 303 669 Fragments arranged by size: 669 303 123 BtsI GCAGTG_nn' Cuts at: 0 541 1053 1095 Size: 541 512 42 Fragments arranged by size: 541 512 42 EaeI y'GGCC_r Cuts at: 0 2 117 1095 Size: 2 115 978 Fragments arranged by size: 978 115 2 EagI C'GGCC_G Cuts at: 0 117 1095 Size: 117 978 EarI CTCTTCn'nnn_ Cuts at: 0 997 1095 Size: 997 98 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 493 544 1095 Size: 493 51 551 Fragments arranged by size: 551 493 51 EcoICRI GAG'CTC Cuts at: 0 759 1007 1095 Size: 759 248 88 Fragments arranged by size: 759 248 88 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 571 607 989 1095 Size: 571 36 382 106 Fragments arranged by size: 571 382 106 36 EcoNI CCTnn'n_nnAGG Cuts at: 0 451 1095 Size: 451 644 EcoO109I rG'GnC_Cy Cuts at: 0 44 45 456 684 788 1095 Size: 44 1 411 228 104 307 Fragments arranged by size: 411 307 228 104 44 1 HaeII r_GCGC'y Cuts at: 0 16 220 396 1095 Size: 16 204 176 699 Fragments arranged by size: 699 204 176 16 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 448 480 947 962 979 994 1095 Size: 448 32 467 15 17 15 101 Fragments arranged by size: 467 448 101 32 17 15 15 KasI G'GCGC_C Cuts at: 0 12 216 1095 Size: 12 204 879 Fragments arranged by size: 879 204 12 KpnI G_GTAC'C Cuts at: 0 411 1095 Size: 411 684 MslI CAynn'nnrTG Cuts at: 0 103 142 1095 Size: 103 39 953 Fragments arranged by size: 953 103 39 MspA1I CmG'CkG Cuts at: 0 122 213 404 475 726 1095 Size: 122 91 191 71 251 369 Fragments arranged by size: 369 251 191 122 91 71 NarI GG'CG_CC Cuts at: 0 13 217 1095 Size: 13 204 878 Fragments arranged by size: 878 204 13 NdeI CA'TA_TG Cuts at: 0 635 1095 Size: 635 460 PasI CC'CwG_GG Cuts at: 0 1059 1095 Size: 1059 36 PflMI CCAn_nnn'nTGG Cuts at: 0 204 1095 Size: 204 891 PpuMI rG'GwC_Cy Cuts at: 0 456 788 1095 Size: 456 332 307 Fragments arranged by size: 456 332 307 PspOMI G'GGCC_C Cuts at: 0 44 683 1095 Size: 44 639 412 Fragments arranged by size: 639 412 44 PstI C_TGCA'G Cuts at: 0 611 780 1095 Size: 611 169 315 Fragments arranged by size: 611 315 169 SacI G_AGCT'C Cuts at: 0 761 1009 1095 Size: 761 248 86 Fragments arranged by size: 761 248 86 SacII CC_GC'GG Cuts at: 0 123 214 405 476 1095 Size: 123 91 191 71 619 Fragments arranged by size: 619 191 123 91 71 SapI GCTCTTCn'nnn_ Cuts at: 0 997 1095 Size: 997 98 SbfI CC_TGCA'GG Cuts at: 0 780 1095 Size: 780 315 SfcI C'TryA_G Cuts at: 0 607 776 1095 Size: 607 169 319 Fragments arranged by size: 607 319 169 SfoI GGC'GCC Cuts at: 0 14 218 1095 Size: 14 204 877 Fragments arranged by size: 877 204 14 StuI AGG'CCT Cuts at: 0 774 1095 Size: 774 321 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 308 514 1095 Size: 308 206 581 Fragments arranged by size: 581 308 206 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 544 576 1095 Size: 544 32 519 Fragments arranged by size: 544 519 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 364 921 1095 Size: 364 557 174 Fragments arranged by size: 557 364 174 Enzymes that do cut: AarI Acc65I AleI ApaI AvaI BanI BanII BbeI BbsI BglI Bme1580I BmgBI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI BssSI BstAPI Bsu36I BtgI BtgZI BtsI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI KpnI MslI MspA1I NarI NdeI PasI PflMI PpuMI PspOMI PstI SacI SacII SapI SbfI SfcI SfoI StuI TaqII TstI XcmI Enzymes that do not cut: AatII AccI AclI AcuI AfeI AflII AflIII AgeI AhdI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmrI BmtI BpuEI BsaBI BsiWI BsmI BspEI BspHI BsrBI BsrGI BssHII BstBI BstEII BstXI BstYI BstZ17I ClaI CspCI DraI DraIII DrdI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI MfeI MluI MmeI MscI NaeI NcoI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SalI SanDI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StyI SwaI TatI Tth111I XbaI XhoI XmaI XmnI ZraI