Restriction map for
pFN21AB6604
(Linear) (Six-base) MAPSORT of: KIBB6604.seq Check: 2715 from: 1 to: 1293
KIBB6604.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 315 1073 1270 1293
Size: 75 240 758 197 23
Fragments arranged by size:
758 240 197 75 23
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1220 1293
Size: 1220 73
AlwNI CAG_nnn'CTG
Cuts at: 0 1143 1293
Size: 1143 150
ApaI G_GGCC'C
Cuts at: 0 293 346 809 1293
Size: 293 53 463 484
Fragments arranged by size:
484 463 293 53
ApaLI G'TGCA_C
Cuts at: 0 109 1293
Size: 109 1184
ApoI r'AATT_y
Cuts at: 0 433 517 1293
Size: 433 84 776
Fragments arranged by size:
776 433 84
AvaI C'yCGr_G
Cuts at: 0 114 1293
Size: 114 1179
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 624 657 1293
Size: 624 33 636
Fragments arranged by size:
636 624 33
BanI G'GyrC_C
Cuts at: 0 27 54 131 234 1170 1293
Size: 27 27 77 103 936 123
Fragments arranged by size:
936 123 103 77 27 27
BanII G_rGCy'C
Cuts at: 0 293 346 809 1293
Size: 293 53 463 484
Fragments arranged by size:
484 463 293 53
BbeI G_GCGC'C
Cuts at: 0 31 135 1293
Size: 31 104 1158
Fragments arranged by size:
1158 104 31
BbsI GAAGACnn'nnnn_
Cuts at: 0 321 1293
Size: 321 972
BglI GCCn_nnn'nGGC
Cuts at: 0 952 1293
Size: 952 341
Bme1580I G_kGCm'C
Cuts at: 0 57 113 293 346 809 1175 1293
Size: 57 56 180 53 463 366 118
Fragments arranged by size:
463 366 180 118 57 56 53
BmrI ACTGGGnnnn_n'
Cuts at: 0 711 1293
Size: 711 582
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 129 192 1293
Size: 129 63 1101
Fragments arranged by size:
1101 129 63
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 577 1293
Size: 199 378 716
Fragments arranged by size:
716 378 199
BsaI GGTCTCn'nnnn_
Cuts at: 0 1013 1293
Size: 1013 280
BsaAI yAC'GTr
Cuts at: 0 6 1293
Size: 6 1287
BsaHI Gr'CG_yC
Cuts at: 0 28 132 386 916 1293
Size: 28 104 254 530 377
Fragments arranged by size:
530 377 254 104 28
BsaWI w'CCGG_w
Cuts at: 0 467 521 569 872 1293
Size: 467 54 48 303 421
Fragments arranged by size:
467 421 303 54 48
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 292 673 1293
Size: 292 381 620
Fragments arranged by size:
620 381 292
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1156 1293
Size: 1156 137
BsiHKAI G_wGCw'C
Cuts at: 0 113 593 602 1293
Size: 113 480 9 691
Fragments arranged by size:
691 480 113 9
BsmBI CGTCTCn'nnnn_
Cuts at: 0 556 742 1293
Size: 556 186 551
Fragments arranged by size:
556 551 186
Bsp1286I G_dGCh'C
Cuts at: 0 57 113 293 346 593 602 809 1175
Size: 57 56 180 53 247 9 207 366
Cuts at: 1175 1293
Size: 118
Fragments arranged by size:
366 247 207 180 118 57 56 53
9
BspEI T'CCGG_A
Cuts at: 0 521 569 1293
Size: 521 48 724
Fragments arranged by size:
724 521 48
BspHI T'CATG_A
Cuts at: 0 398 1293
Size: 398 895
BsrBI CCG'CTC
Cuts at: 0 150 908 1293
Size: 150 758 385
Fragments arranged by size:
758 385 150
BsrFI r'CCGG_y
Cuts at: 0 128 1293
Size: 128 1165
BsrGI T'GTAC_A
Cuts at: 0 257 1293
Size: 257 1036
BssSI C'ACGA_G
Cuts at: 0 415 997 1293
Size: 415 582 296
Fragments arranged by size:
582 415 296
BstXI CCAn_nnnn'nTGG
Cuts at: 0 273 1293
Size: 273 1020
BstYI r'GATC_y
Cuts at: 0 170 485 524 618 1293
Size: 170 315 39 94 675
Fragments arranged by size:
675 315 170 94 39
BtgI C'CryG_G
Cuts at: 0 266 715 848 878 1293
Size: 266 449 133 30 415
Fragments arranged by size:
449 415 266 133 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1029 1293
Size: 1029 264
BtsI GCAGTG_nn'
Cuts at: 0 13 1293
Size: 13 1280
DraIII CAC_nnn'GTG
Cuts at: 0 705 1293
Size: 705 588
EaeI y'GGCC_r
Cuts at: 0 83 297 353 944 1293
Size: 83 214 56 591 349
Fragments arranged by size:
591 349 214 83 56
EarI CTCTTCn'nnn_
Cuts at: 0 628 1293
Size: 628 665
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 66 1293
Size: 66 1227
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 199 315 577 1073 1270 1293
Size: 75 124 116 262 496 197 23
Fragments arranged by size:
496 262 197 124 116 75 23
EcoO109I rG'GnC_Cy
Cuts at: 0 342 343 806 1293
Size: 342 1 463 487
Fragments arranged by size:
487 463 342 1
EcoRI G'AATT_C
Cuts at: 0 433 517 1293
Size: 433 84 776
Fragments arranged by size:
776 433 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 345 453 840 1293
Size: 345 108 387 453
Fragments arranged by size:
453 387 345 108
HaeII r_GCGC'y
Cuts at: 0 31 49 135 1293
Size: 31 18 86 1158
Fragments arranged by size:
1158 86 31 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1219 1251 1293
Size: 1219 32 42
Fragments arranged by size:
1219 42 32
HincII GTy'rAC
Cuts at: 0 411 1293
Size: 411 882
KasI G'GCGC_C
Cuts at: 0 27 131 1293
Size: 27 104 1162
Fragments arranged by size:
1162 104 27
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1262 1293
Size: 1262 31
MscI TGG'CCA
Cuts at: 0 355 946 1293
Size: 355 591 347
Fragments arranged by size:
591 355 347
MslI CAynn'nnrTG
Cuts at: 0 244 271 397 1293
Size: 244 27 126 896
Fragments arranged by size:
896 244 126 27
MspA1I CmG'CkG
Cuts at: 0 717 1293
Size: 717 576
NarI GG'CG_CC
Cuts at: 0 28 132 1293
Size: 28 104 1161
Fragments arranged by size:
1161 104 28
NcoI C'CATG_G
Cuts at: 0 266 1293
Size: 266 1027
PasI CC'CwG_GG
Cuts at: 0 339 1293
Size: 339 954
PflMI CCAn_nnn'nTGG
Cuts at: 0 705 1293
Size: 705 588
PfoI T'CCnGG_A
Cuts at: 0 542 593 1293
Size: 542 51 700
Fragments arranged by size:
700 542 51
PmlI CAC'GTG
Cuts at: 0 6 1293
Size: 6 1287
PshAI GACnn'nnGTC
Cuts at: 0 1144 1293
Size: 1144 149
PspOMI G'GGCC_C
Cuts at: 0 289 342 805 1293
Size: 289 53 463 488
Fragments arranged by size:
488 463 289 53
PspXI vC'TCGA_Gb
Cuts at: 0 114 1293
Size: 114 1179
PstI C_TGCA'G
Cuts at: 0 737 1293
Size: 737 556
SacII CC_GC'GG
Cuts at: 0 718 1293
Size: 718 575
SfcI C'TryA_G
Cuts at: 0 733 1293
Size: 733 560
SfoI GGC'GCC
Cuts at: 0 29 133 1293
Size: 29 104 1160
Fragments arranged by size:
1160 104 29
SgrAI Cr'CCGG_yG
Cuts at: 0 128 1293
Size: 128 1165
SmlI C'TyrA_G
Cuts at: 0 114 1293
Size: 114 1179
StuI AGG'CCT
Cuts at: 0 985 1293
Size: 985 308
StyI C'CwwG_G
Cuts at: 0 266 1293
Size: 266 1027
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 252 458 693 1293
Size: 252 206 235 600
Fragments arranged by size:
600 252 235 206
TatI w'GTAC_w
Cuts at: 0 257 1293
Size: 257 1036
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 47 79 1293
Size: 47 32 1214
Fragments arranged by size:
1214 47 32
Tth111I GACn'n_nGTC
Cuts at: 0 394 1293
Size: 394 899
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 246 1293
Size: 246 1047
XhoI C'TCGA_G
Cuts at: 0 114 1293
Size: 114 1179
Enzymes that do cut:
AcuI AlfI AlwNI ApaI ApaLI ApoI AvaI BaeI
BanI BanII BbeI BbsI BglI Bme1580I BmrI BplI
BpmI BsaI BsaAI BsaHI BsaWI BseRI BsgI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BsrBI BsrFI BsrGI BssSI
BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EarI
EciI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HincII
KasI MmeI MscI MslI MspA1I NarI NcoI PasI
PflMI PfoI PmlI PshAI PspOMI PspXI PstI SacII
SfcI SfoI SgrAI SmlI StuI StyI TaqII TatI
TstI Tth111I XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI AscI AseI AsiSI AvrII
BamHI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
BmgBI BmtI Bpu10I BpuEI BsaBI BsaXI BseYI BsiEI
BsiWI BsmI BspMI BsrDI BssHII BstAPI BstBI BstEII
BstZ17I Bsu36I ClaI CspCI DraI DrdI EagI EcoICRI
EcoNI EcoRV FseI FspI FspAI HindIII HpaI KpnI
MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PmeI PpiI PpuMI PsiI
PsrI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SmaI SnaBI SpeI
SphI SrfI SspI SwaI XbaI XmaI XmnI ZraI