################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspI AviII BamHI BbeI BbrPI BbvCI BcgI BfrI BglI BlpI Bpu1102I BsaHI BsaI BsaWI BseAI BsgI BsiWI BsmBI BspDI BspEI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoT22I FseI FspI HaeII HhaI Hin1I HinP1I HpaI KasI KpnI MfeI MluI MroI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccII 1047 CGCG CG^CG AhdI 1380 GACATCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 271 GGGCCC GGGCC^C AspEI 1380 GACATCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 609 CCCGAG C^[CT]CG[GA]G AvrII 714 CCTAGG C^CTAGG BalI 899 TGGCCA TGG^CCA BciVI 1507 GGATAC GTATCC(6/5) BglII 686 AGATCT A^GATCT BlnI 714 CCTAGG C^CTAGG BpmI 1253 CTGGAG CTGGAG(16/14) BsiEI 1614 CGACCG CG[GA][CT]^CG BsmI 1339 GAATGC GAATGC(1/-1) BsoBI 609 CCCGAG C^[CT]CG[GA]G BspHI 1280 TCATGA T^CATGA BstEII 796 GGTGACC G^GT[ACGT]ACC BstPI 796 GGTGACC G^GT[ACGT]ACC BstUI 1047 CGCG CG^CG EaeI 899 TGGCCA [CT]^GGCC[GA] Eam1105I 1380 GACATCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 796 GGTGACC G^GT[ACGT]ACC EcoRV 535 GATATC GAT^ATC FauI 1045 CCCGC CCCGC(4/6) HgaI 767 GCGTC GACGC(5/10) KspI 1046 CCGCGG CCGC^GG MluNI 899 TGGCCA TGG^CCA MscI 899 TGGCCA TGG^CCA MvnI 1047 CGCG CG^CG NcoI 542 CCATGG C^CATGG NdeI 925 CATATG CA^TATG NspI 870 GCATGC [GA]CATG^[CT] PpuMI 1517 AGGACCC [GA]G^G[AT]CC[CT] PshAI 1573 GACATTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 960 CAGCTG CAG^CTG RcaI 1280 TCATGA T^CATGA SacII 1046 CCGCGG CCGC^GG SfcI 306 CTACAG C^T[GA][CT]AG SphI 870 GCATGC GCATG^C SspI 829 AATATT AAT^ATT TfiI 980 GATTC G^A[AT]TC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AcsI 1144 AAATTC [GA]^AATT[CT] 1709 AAATTT AflIII 458 ACACGT A^C[GA][CT]GT 1496 ACGTGT ApoI 1144 AAATTC [GA]^AATT[CT] 1709 AAATTT BanI 1050 GGTGCC G^G[CT][GA]CC 1398 GGTGCC BclI 92 TGATCA T^GATCA 623 TGATCA BcnI 42 CCCGG CC^[GC]GG 1293 CCGGG BfaI 715 CTAG C^TAG 749 CTAG BsaAI 459 CACGTA [CT]AC^GT[GA] 1417 TACGTG BsaBI 51 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 311 GATGGCTATC BseRI 158 GAGGAG GAGGAG(10/8) 1988 GAGGAG BsmAI 1571 GAGAC GTCTC(1/5) 362 GTCTC BsrDI 1656 CATTGC GCAATG(2/0) 1300 GCAATG DraII 271 GGGCCCT [GA]G^G[ACGT]CC[CT] 1517 AGGACCC DrdI 394 GACCAGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 760 GACCTGAGCGTC DsaI 542 CCATGG C^C[GA][CT]GG 1046 CCGCGG EarI 119 CTCTTC CTCTTC(1/4) 672 GAAGAG EcoO109I 271 GGGCCCT [GA]G^G[ACGT]CC[CT] 1517 AGGACCC FbaI 92 TGATCA T^GATCA 623 TGATCA HapII 43 CCGG C^CGG 1293 CCGG HincII 523 GTCAAC GT[CT]^[GA]AC 1694 GTCAAC HindII 523 GTCAAC GT[CT]^[GA]AC 1694 GTCAAC HindIII 1322 AAGCTT A^AGCTT 1795 AAGCTT HpaII 43 CCGG C^CGG 1293 CCGG Ksp632I 119 CTCTTC CTCTTC(1/4) 672 GAAGAG MaeI 715 CTAG C^TAG 749 CTAG MamI 51 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 311 GATGGCTATC MslI 627 CATCCTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 871 CATGCTTATG MspA1I 960 CAGCTG C[AC]G^C[GT]G 1046 CCGCGG MspI 43 CCGG C^CGG 1293 CCGG NciI 42 CCCGG CC^[GC]GG 1293 CCGGG TaqI 1056 TCGA T^CGA 1613 TCGA TthHB8I 1056 TCGA T^CGA 1613 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AciI AfaI AluI AlwI Asp700I AspHI AvaII BanII BbsI BbvI BmyI BpuAI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I Bsp1407I BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SspBI StyI Tru9I TseI Tsp45I Tsp509I TspRI XhoII XmnI