(Circular) (Six-base) MAPSORT of: pFN21AE4558.seq Check: 629 from: 1 to: 6754 pFN21AE4558 6754 bp With 182 enzymes: SgfI * March 28, 2012 14:15 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4564 278 Size: 53 83 186 936 3028 2468 Fragments arranged by size: 3028 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4097 1937 Size: 2160 4594 Acc65I G'GTAC_C Cuts at: 4075 4075 Size: 6754 AclI AA'CG_TT Cuts at: 1563 4881 5254 1563 Size: 3318 373 3063 Fragments arranged by size: 3318 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2642 2681 2719 3264 3539 3629 4817 6201 Size: 1010 39 38 545 275 90 1188 1384 Cuts at: 6201 6469 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1188 1010 545 275 268 90 39 38 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6557 AflIII A'CryG_T Cuts at: 1203 2463 3501 5653 6630 1203 Size: 1260 1038 2152 977 1327 Fragments arranged by size: 2152 1327 1260 1038 977 AgeI A'CCGG_T Cuts at: 5585 5585 Size: 6754 AhdI GACnn_n'nnGTC Cuts at: 3390 5483 3390 Size: 2093 4661 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6754 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6754 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3747 3779 3747 Size: 32 6722 AlwNI CAG_nnn'CTG Cuts at: 4497 6069 4497 Size: 1572 5182 ApaI G_GGCC'C Cuts at: 2280 2280 Size: 6754 ApaLI G'TGCA_C Cuts at: 4811 5967 4811 Size: 1156 5598 ApoI r'AATT_y Cuts at: 1493 3149 3714 4063 4289 4441 6550 1493 Size: 1656 565 349 226 152 2109 1697 Fragments arranged by size: 2109 1697 1656 565 349 226 152 AseI AT'TA_AT Cuts at: 160 1032 5308 160 Size: 872 4276 1606 Fragments arranged by size: 4276 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6754 AvaI C'yCGr_G Cuts at: 1870 1943 2614 4079 1870 Size: 73 671 1465 4545 Fragments arranged by size: 4545 1465 671 73 AvrII C'CTAG_G Cuts at: 2719 2719 Size: 6754 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3486 3519 3486 Size: 33 6721 BamHI G'GATC_C Cuts at: 4084 4451 4084 Size: 367 6387 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3055 3403 4075 5530 618 Size: 325 217 627 1268 348 672 1455 1842 Fragments arranged by size: 1842 1455 1268 672 627 348 325 217 BanII G_rGCy'C Cuts at: 1391 2280 2889 3818 3966 4073 1391 Size: 889 609 929 148 107 4072 Fragments arranged by size: 4072 929 889 609 148 107 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2769 3032 3539 3873 928 Size: 971 870 263 507 334 3809 Fragments arranged by size: 3809 971 870 507 334 263 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4945 4979 1903 Size: 34 50 34 2924 34 3678 Fragments arranged by size: 3678 2924 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3506 4646 5856 3506 Size: 1140 1210 4404 Fragments arranged by size: 4404 1210 1140 BclI T'GATC_A Cuts at: 1548 2097 2628 1548 Size: 549 531 5674 Fragments arranged by size: 5674 549 531 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5365 136 Size: 107 122 71 631 605 3693 1525 Fragments arranged by size: 3693 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2691 6749 2691 Size: 4058 2696 BlpI GC'TnA_GC Cuts at: 4171 4171 Size: 6754 Bme1580I G_kGCm'C Cuts at: 1790 2280 3408 4815 5971 1790 Size: 490 1128 1407 1156 2573 Fragments arranged by size: 2573 1407 1156 1128 490 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6754 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4485 5443 442 Size: 416 53 83 399 24 3068 958 1753 Fragments arranged by size: 3068 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6754 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3279 5414 1239 Size: 2040 2135 2579 Fragments arranged by size: 2579 2135 2040 Bpu10I CC'TnA_GC Cuts at: 2768 2768 Size: 6754 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4879 5744 6042 4879 Size: 865 298 5591 Fragments arranged by size: 5591 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5417 882 Size: 617 3918 2219 Fragments arranged by size: 3918 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2466 3424 4545 493 Size: 711 1262 958 1121 2702 Fragments arranged by size: 2702 1262 1121 958 711 BsaBI GATnn'nnATC Cuts at: 2060 2320 6748 2060 Size: 260 4428 2066 Fragments arranged by size: 4428 2066 260 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4561 4943 5578 275 Size: 53 83 186 936 3028 382 635 1451 Fragments arranged by size: 3028 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5187 5585 5859 6006 6417 5187 Size: 398 274 147 411 5524 Fragments arranged by size: 5524 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2964 2994 3747 3777 1178 Size: 30 165 30 1561 30 753 30 4155 Fragments arranged by size: 4155 1561 753 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2178 4008 1186 Size: 992 1830 3932 Fragments arranged by size: 3932 1830 992 BseYI C'CCAG_C Cuts at: 5957 6453 5957 Size: 496 6258 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6181 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3622 4204 4965 5993 1540 Size: 275 186 1621 582 761 1028 2301 Fragments arranged by size: 2301 1621 1028 761 582 275 186 BsiHKAI G_wGCw'C Cuts at: 2170 2733 4044 4815 4900 5971 2170 Size: 563 1311 771 85 1071 2953 Fragments arranged by size: 2953 1311 1071 771 563 85 BsmI GAATG_Cn' Cuts at: 3350 4265 4358 3350 Size: 915 93 5746 Fragments arranged by size: 5746 915 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2170 2280 2733 2889 3408 3818 3966 Size: 399 380 110 453 156 519 410 148 Cuts at: 3966 4044 4073 4815 4900 5971 1391 Size: 78 29 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 519 453 410 399 380 156 148 110 85 78 29 BspEI T'CCGG_A Cuts at: 6417 6417 Size: 6754 BspHI T'CATG_A Cuts at: 3285 4643 6373 3285 Size: 1358 1730 3666 Fragments arranged by size: 3666 1730 1358 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4109 844 Size: 3265 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4641 1394 Size: 429 2818 3507 Fragments arranged by size: 3507 2818 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3312 3660 5249 5423 65 Size: 1183 2064 348 1589 174 1396 Fragments arranged by size: 2064 1589 1396 1183 348 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5398 5585 1700 Size: 110 3588 187 2869 Fragments arranged by size: 3588 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2947 2961 3359 3721 96 Size: 2851 14 398 362 3129 Fragments arranged by size: 3129 2851 398 362 14 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6754 BssSI C'ACGA_G Cuts at: 4814 5826 4814 Size: 1012 5742 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6754 BstBI TT'CG_AA Cuts at: 5558 5558 Size: 6754 BstEII G'GTnAC_C Cuts at: 1184 2801 1184 Size: 1617 5137 BstYI r'GATC_y Cuts at: 1972 2691 3075 3216 4084 4451 4836 4853 6305 Size: 719 384 141 868 367 385 17 1452 Cuts at: 6305 6391 6403 6749 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 868 719 385 384 367 346 141 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2547 3051 513 Size: 552 1482 504 4216 Fragments arranged by size: 4216 1482 552 504 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6015 Fragments arranged by size: 6015 683 56 BtsI GCAGTG_nn' Cuts at: 4270 5064 5084 4270 Size: 794 20 5940 Fragments arranged by size: 5940 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6754 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6719 DraI TTT'AAA Cuts at: 4058 4058 Size: 6754 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2405 2771 5761 809 Size: 507 1089 366 2990 1802 Fragments arranged by size: 2990 1802 1089 507 366 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2904 4201 5090 Size: 54 1425 50 275 18 1074 1297 889 Cuts at: 5090 6719 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1297 1074 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4201 1537 Size: 275 2389 4090 Fragments arranged by size: 4090 2389 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2130 2672 4684 1353 Size: 777 542 2012 3423 Fragments arranged by size: 3423 2012 777 542 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3249 5341 5715 5861 387 Size: 1064 138 1660 2092 374 146 1280 Fragments arranged by size: 2092 1660 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2642 2681 2719 3264 3279 3539 3629 Size: 393 1010 39 38 545 15 260 90 Cuts at: 3629 4817 5414 6201 6469 1239 Size: 1188 597 787 268 1524 Fragments arranged by size: 1524 1188 1010 787 597 545 393 268 260 90 39 38 15 EcoO109I rG'GnC_Cy Cuts at: 2277 3523 2277 Size: 1246 5508 EcoRI G'AATT_C Cuts at: 4063 4441 4063 Size: 378 6376 EcoRV GAT'ATC Cuts at: 2542 2542 Size: 6754 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3127 3139 3223 3835 3127 Size: 12 84 612 6046 Fragments arranged by size: 6046 612 84 12 FspI TGC'GCA Cuts at: 5260 5260 Size: 6754 HaeII r_GCGC'y Cuts at: 1678 5901 1678 Size: 4223 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2067 2099 2681 2713 2812 Size: 32 135 32 495 32 582 32 99 Cuts at: 2812 2844 3371 3376 3403 3408 5395 5427 5469 Size: 32 527 5 27 5 1987 32 42 Cuts at: 5469 5501 1373 Size: 32 2626 Fragments arranged by size: 2626 1987 582 527 495 135 99 42 32 32 32 32 32 32 32 27 5 5 HincII GTy'rAC Cuts at: 669 1657 1938 2530 3701 4098 4344 669 Size: 988 281 592 1171 397 246 3079 Fragments arranged by size: 3079 1171 988 592 397 281 246 HindIII A'AGCT_T Cuts at: 748 3327 3800 748 Size: 2579 473 3702 Fragments arranged by size: 3702 2579 473 HpaI GTT'AAC Cuts at: 4344 4344 Size: 6754 KpnI G_GTAC'C Cuts at: 4079 4079 Size: 6754 MfeI C'AATT_G Cuts at: 4353 4353 Size: 6754 MluI A'CGCG_T Cuts at: 6630 6630 Size: 6754 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2066 2962 5868 6052 1305 Size: 761 896 2906 184 2007 Fragments arranged by size: 2906 2007 896 761 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2906 10 Size: 54 1425 343 1074 3858 Fragments arranged by size: 3858 1425 1074 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2636 2880 4712 5071 5230 518 Size: 648 1470 244 1832 359 159 2042 Fragments arranged by size: 2042 1832 1470 648 359 244 159 MspA1I CmG'CkG Cuts at: 1606 2967 3053 4170 4847 5995 6240 1606 Size: 1361 86 1117 677 1148 245 2120 Fragments arranged by size: 2120 1361 1148 1117 677 245 86 NcoI C'CATG_G Cuts at: 513 1065 2547 513 Size: 552 1482 4720 Fragments arranged by size: 4720 1482 552 NdeI CA'TA_TG Cuts at: 387 2931 387 Size: 2544 4210 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6754 NotI GC'GGCC_GC Cuts at: 4201 4201 Size: 6754 NspI r_CATG'y Cuts at: 1132 1229 2879 4112 5657 1132 Size: 97 1650 1233 1545 2229 Fragments arranged by size: 2229 1650 1545 1233 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6754 PciI A'CATG_T Cuts at: 5653 5653 Size: 6754 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6754 PfoI T'CCnGG_A Cuts at: 3219 4047 3219 Size: 828 5926 PmeI GTTT'AAAC Cuts at: 4058 4058 Size: 6754 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3747 3779 4823 4855 6336 6368 3747 Size: 32 1044 32 1481 32 4133 Fragments arranged by size: 4133 1481 1044 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3523 3523 Size: 6754 PshAI GACnn'nnGTC Cuts at: 1613 3582 1613 Size: 1969 4785 PsiI TTA'TAA Cuts at: 2385 4324 2385 Size: 1939 4815 PspOMI G'GGCC_C Cuts at: 2276 2276 Size: 6754 PstI C_TGCA'G Cuts at: 830 4106 830 Size: 3276 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6754 PvuII CAG'CTG Cuts at: 2967 2967 Size: 6754 SacII CC_GC'GG Cuts at: 3054 3054 Size: 6754 SalI G'TCGA_C Cuts at: 1936 4096 1936 Size: 2160 4594 SbfI CC_TGCA'GG Cuts at: 4106 4106 Size: 6754 ScaI AGT'ACT Cuts at: 5002 6714 5002 Size: 1712 5042 SfcI C'TryA_G Cuts at: 826 1045 2311 4102 5237 5918 6109 826 Size: 219 1266 1791 1135 681 191 1471 Fragments arranged by size: 1791 1471 1266 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6754 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6754 SmaI CCC'GGG Cuts at: 1872 4081 1872 Size: 2209 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4858 5759 6021 820 Size: 197 926 2915 901 262 1553 Fragments arranged by size: 2915 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4545 493 Size: 4052 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6754 SphI G_CATG'C Cuts at: 1132 2879 4112 1132 Size: 1747 1233 3774 Fragments arranged by size: 3774 1747 1233 SspI AAT'ATT Cuts at: 5 52 2836 4678 5 Size: 47 2784 1842 2081 Fragments arranged by size: 2784 2081 1842 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6754 StyI C'CwwG_G Cuts at: 513 1065 2499 2547 2719 2992 3649 4193 513 Size: 552 1434 48 172 273 657 544 3074 Fragments arranged by size: 3074 1434 657 552 544 273 172 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4781 4798 4951 5136 5581 325 Size: 1255 3201 17 153 185 445 1498 Fragments arranged by size: 3201 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2947 2961 3359 Size: 274 80 33 51 1443 970 14 398 Cuts at: 3359 3721 3778 5000 6712 96 Size: 362 57 1222 1712 138 Fragments arranged by size: 1712 1443 1222 970 398 362 274 138 80 57 51 33 14 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2247 2279 2785 2817 3031 3063 3303 3335 3877 Size: 32 506 32 214 32 240 32 542 Cuts at: 3877 3909 6714 6746 2247 Size: 32 2805 32 2255 Fragments arranged by size: 2805 2255 542 506 240 214 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6754 XbaI T'CTAG_A Cuts at: 4090 4090 Size: 6754 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6754 XmaI C'CCGG_G Cuts at: 1870 4079 1870 Size: 2209 4545 XmnI GAAnn'nnTTC Cuts at: 2606 3119 3635 4883 2606 Size: 513 516 1248 4477 Fragments arranged by size: 4477 1248 516 513 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4562 276 Size: 53 83 186 936 3028 2468 Fragments arranged by size: 3028 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI