(Linear) (Six-base) MAPSORT of: KIEE4558.seq Check: 7494 from: 1 to: 2049 KIEE4558.orf With 182 enzymes: SgfI * March 22, 2012 18:22 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 637 676 714 1259 1534 1624 2049 Size: 637 39 38 545 275 90 425 Fragments arranged by size: 637 545 425 275 90 39 38 AflIII A'CryG_T Cuts at: 0 458 1496 2049 Size: 458 1038 553 Fragments arranged by size: 1038 553 458 AhdI GACnn_n'nnGTC Cuts at: 0 1385 2049 Size: 1385 664 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1742 1774 2049 Size: 1742 32 275 Fragments arranged by size: 1742 275 32 ApaI G_GGCC'C Cuts at: 0 275 2049 Size: 275 1774 ApoI r'AATT_y Cuts at: 0 1144 1709 2049 Size: 1144 565 340 Fragments arranged by size: 1144 565 340 AvaI C'yCGr_G Cuts at: 0 609 2049 Size: 609 1440 AvrII C'CTAG_G Cuts at: 0 714 2049 Size: 714 1335 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1481 1514 2049 Size: 1481 33 535 Fragments arranged by size: 1481 535 33 BanI G'GyrC_C Cuts at: 0 1050 1398 2049 Size: 1050 348 651 Fragments arranged by size: 1050 651 348 BanII G_rGCy'C Cuts at: 0 275 884 1813 1961 2049 Size: 275 609 929 148 88 Fragments arranged by size: 929 609 275 148 88 BbsI GAAGACnn'nnnn_ Cuts at: 0 764 1027 1534 1868 2049 Size: 764 263 507 334 181 Fragments arranged by size: 764 507 334 263 181 BciVI GTATCCnnnnn_n' Cuts at: 0 1501 2049 Size: 1501 548 BclI T'GATC_A Cuts at: 0 92 623 2049 Size: 92 531 1426 Fragments arranged by size: 1426 531 92 BglII A'GATC_T Cuts at: 0 686 2049 Size: 686 1363 Bme1580I G_kGCm'C Cuts at: 0 275 1403 2049 Size: 275 1128 646 Fragments arranged by size: 1128 646 275 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1274 2049 Size: 1274 775 Bpu10I CC'TnA_GC Cuts at: 0 763 2049 Size: 763 1286 BsaAI yAC'GTr Cuts at: 0 461 1419 2049 Size: 461 958 630 Fragments arranged by size: 958 630 461 BsaBI GATnn'nnATC Cuts at: 0 55 315 2049 Size: 55 260 1734 Fragments arranged by size: 1734 260 55 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 959 989 1742 1772 2049 Size: 959 30 753 30 277 Fragments arranged by size: 959 753 277 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 173 2003 2049 Size: 173 1830 46 Fragments arranged by size: 1830 173 46 BsiEI CG_ry'CG Cuts at: 0 1617 2049 Size: 1617 432 BsiHKAI G_wGCw'C Cuts at: 0 165 728 2039 2049 Size: 165 563 1311 10 Fragments arranged by size: 1311 563 165 10 BsmI GAATG_Cn' Cuts at: 0 1345 2049 Size: 1345 704 Bsp1286I G_dGCh'C Cuts at: 0 165 275 728 884 1403 1813 1961 2039 Size: 165 110 453 156 519 410 148 78 Cuts at: 2039 2049 Size: 10 Fragments arranged by size: 519 453 410 165 156 148 110 78 10 BspHI T'CATG_A Cuts at: 0 1280 2049 Size: 1280 769 BsrDI GCAATG_nn' Cuts at: 0 1307 1655 2049 Size: 1307 348 394 Fragments arranged by size: 1307 394 348 BsrGI T'GTAC_A Cuts at: 0 942 956 1354 1716 2049 Size: 942 14 398 362 333 Fragments arranged by size: 942 398 362 333 14 BstEII G'GTnAC_C Cuts at: 0 796 2049 Size: 796 1253 BstYI r'GATC_y Cuts at: 0 686 1070 1211 2049 Size: 686 384 141 838 Fragments arranged by size: 838 686 384 141 BtgI C'CryG_G Cuts at: 0 542 1046 2049 Size: 542 504 1003 Fragments arranged by size: 1003 542 504 DrdI GACnn_nn'nnGTC Cuts at: 0 400 766 2049 Size: 400 366 1283 Fragments arranged by size: 1283 400 366 EaeI y'GGCC_r Cuts at: 0 899 2049 Size: 899 1150 EarI CTCTTCn'nnn_ Cuts at: 0 125 667 2049 Size: 125 542 1382 Fragments arranged by size: 1382 542 125 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1244 2049 Size: 1244 805 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 637 676 714 1259 1274 1534 1624 2049 Size: 637 39 38 545 15 260 90 425 Fragments arranged by size: 637 545 425 260 90 39 38 15 EcoO109I rG'GnC_Cy Cuts at: 0 272 1518 2049 Size: 272 1246 531 Fragments arranged by size: 1246 531 272 EcoRV GAT'ATC Cuts at: 0 537 2049 Size: 537 1512 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1122 1134 1218 1830 2049 Size: 1122 12 84 612 219 Fragments arranged by size: 1122 612 219 84 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 62 94 676 708 807 839 1366 1371 Size: 62 32 582 32 99 32 527 5 Cuts at: 1371 1398 1403 2049 Size: 27 5 646 Fragments arranged by size: 646 582 527 99 62 32 32 32 27 5 5 HincII GTy'rAC Cuts at: 0 525 1696 2049 Size: 525 1171 353 Fragments arranged by size: 1171 525 353 HindIII A'AGCT_T Cuts at: 0 1322 1795 2049 Size: 1322 473 254 Fragments arranged by size: 1322 473 254 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 61 957 2049 Size: 61 896 1092 Fragments arranged by size: 1092 896 61 MscI TGG'CCA Cuts at: 0 901 2049 Size: 901 1148 MslI CAynn'nnrTG Cuts at: 0 631 875 2049 Size: 631 244 1174 Fragments arranged by size: 1174 631 244 MspA1I CmG'CkG Cuts at: 0 962 1048 2049 Size: 962 86 1001 Fragments arranged by size: 1001 962 86 NcoI C'CATG_G Cuts at: 0 542 2049 Size: 542 1507 NdeI CA'TA_TG Cuts at: 0 926 2049 Size: 926 1123 NspI r_CATG'y Cuts at: 0 874 2049 Size: 874 1175 PfoI T'CCnGG_A Cuts at: 0 1214 2042 2049 Size: 1214 828 7 Fragments arranged by size: 1214 828 7 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1742 1774 2049 Size: 1742 32 275 Fragments arranged by size: 1742 275 32 PpuMI rG'GwC_Cy Cuts at: 0 1518 2049 Size: 1518 531 PshAI GACnn'nnGTC Cuts at: 0 1577 2049 Size: 1577 472 PsiI TTA'TAA Cuts at: 0 380 2049 Size: 380 1669 PspOMI G'GGCC_C Cuts at: 0 271 2049 Size: 271 1778 PvuII CAG'CTG Cuts at: 0 962 2049 Size: 962 1087 SacII CC_GC'GG Cuts at: 0 1049 2049 Size: 1049 1000 SfcI C'TryA_G Cuts at: 0 306 2049 Size: 306 1743 SphI G_CATG'C Cuts at: 0 874 2049 Size: 874 1175 SspI AAT'ATT Cuts at: 0 831 2049 Size: 831 1218 StyI C'CwwG_G Cuts at: 0 494 542 714 987 1644 2049 Size: 494 48 172 273 657 405 Fragments arranged by size: 657 494 405 273 172 48 TatI w'GTAC_w Cuts at: 0 942 956 1354 1716 1773 2049 Size: 942 14 398 362 57 276 Fragments arranged by size: 942 398 362 276 57 14 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 242 274 780 812 1026 1058 1298 1330 Size: 242 32 506 32 214 32 240 32 Cuts at: 1330 1872 1904 2049 Size: 542 32 145 Fragments arranged by size: 542 506 242 240 214 145 32 32 32 32 32 XmnI GAAnn'nnTTC Cuts at: 0 601 1114 1630 2049 Size: 601 513 516 419 Fragments arranged by size: 601 516 513 419 Enzymes that do cut: AcuI AflIII AhdI AloI ApaI ApoI AvaI AvrII BaeI BanI BanII BbsI BciVI BclI BglII Bme1580I BpmI Bpu10I BsaAI BsaBI BsaXI BseRI BsiEI BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrGI BstEII BstYI BtgI DrdI EaeI EarI EciI Eco57MI EcoO109I EcoRV FalI Hin4I HincII HindIII MmeI MscI MslI MspA1I NcoI NdeI NspI PfoI PpiI PpuMI PshAI PsiI PspOMI PvuII SacII SfcI SphI SspI StyI TatI TstI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AleI AlfI AlwNI ApaLI AscI AseI AsiSI BamHI BbeI BbvCI BcgI BfrBI BglI BlpI BmgBI BmrI BmtI BplI BpuEI BsaI BsaHI BsaWI BseYI BsgI BsiWI BsmBI BspEI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII EagI EcoICRI EcoNI EcoRI FseI FspI FspAI HaeII HpaI KasI KpnI MfeI MluI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PspXI PsrI PstI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI StuI SwaI TaqII Tth111I XbaI XcmI XhoI XmaI ZraI