Restriction map for
pFN21AE5113
(Circular) (Six-base) MAPSORT of: pFN21AE5113.seq Check: 2490 from: 1 to: 5179
pFN21AE5113 5179 bp
With 182 enzymes: SgfI *
January 30, 2014 17:12 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2364 2989 278
Size: 53 83 186 936 828 625 2468
Fragments arranged by size:
2468 936 828 625 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2522 1937
Size: 585 4594
Acc65I G'GTAC_C
Cuts at: 2500 2500
Size: 5179
AclI AA'CG_TT
Cuts at: 1563 3306 3679 1563
Size: 1743 373 3063
Fragments arranged by size:
3063 1743 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2338 3242 4626 4894 1632
Size: 706 904 1384 268 1917
Fragments arranged by size:
1917 1384 904 706 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4982
AflIII A'CryG_T
Cuts at: 1203 2328 4078 5055 1203
Size: 1125 1750 977 1327
Fragments arranged by size:
1750 1327 1125 977
AgeI A'CCGG_T
Cuts at: 4010 4010
Size: 5179
AhdI GACnn_n'nnGTC
Cuts at: 3908 3908
Size: 5179
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5179
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2432 1184
Size: 1248 3931
AlwNI CAG_nnn'CTG
Cuts at: 2922 4494 2922
Size: 1572 3607
ApaLI G'TGCA_C
Cuts at: 3236 4392 3236
Size: 1156 4023
ApoI r'AATT_y
Cuts at: 1493 2488 2714 2866 4975 1493
Size: 995 226 152 2109 1697
Fragments arranged by size:
2109 1697 995 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3733 160
Size: 872 2701 1606
Fragments arranged by size:
2701 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5179
AvaI C'yCGr_G
Cuts at: 1870 1943 2504 1870
Size: 73 561 4545
Fragments arranged by size:
4545 561 73
BamHI G'GATC_C
Cuts at: 2217 2509 2876 2217
Size: 292 367 4520
Fragments arranged by size:
4520 367 292
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2162 2453 2500 3955 618
Size: 325 217 627 375 291 47 1455 1842
Fragments arranged by size:
1842 1455 627 375 325 291 217 47
BanII G_rGCy'C
Cuts at: 1391 2498 1391
Size: 1107 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4208
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3370 3404 1903
Size: 34 1433 34 3678
Fragments arranged by size:
3678 1433 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3071 4281 3071
Size: 1210 3969
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5179
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3790 136
Size: 107 122 71 631 605 2118 1525
Fragments arranged by size:
2118 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5174 5174
Size: 5179
BlpI GC'TnA_GC
Cuts at: 2596 2596
Size: 5179
Bme1580I G_kGCm'C
Cuts at: 1790 2165 3240 4396 1790
Size: 375 1075 1156 2573
Fragments arranged by size:
2573 1156 1075 375
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5179
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2910 3868 442
Size: 416 53 83 399 24 1493 958 1753
Fragments arranged by size:
1753 1493 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5179
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3839 1239
Size: 2600 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2417 3304 4169 4467 2417
Size: 887 865 298 3129
Fragments arranged by size:
3129 887 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3842 882
Size: 617 2343 2219
Fragments arranged by size:
2343 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2970 493
Size: 711 1766 2702
Fragments arranged by size:
2702 1766 711
BsaBI GATnn'nnATC
Cuts at: 5173 5173
Size: 5179
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2361 2986 3368 4003
Size: 53 83 186 936 828 625 382 635
Cuts at: 4003 275
Size: 1451
Fragments arranged by size:
1451 936 828 635 625 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3612 4010 4284 4431 4842 3612
Size: 398 274 147 411 3949
Fragments arranged by size:
3949 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4954
Fragments arranged by size:
4954 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5179
BseYI C'CCAG_C
Cuts at: 2077 4382 4878 2077
Size: 2305 496 2378
Fragments arranged by size:
2378 2305 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2267 1163
Size: 573 531 4075
Fragments arranged by size:
4075 573 531
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2629 3390 4418 1540
Size: 275 186 628 761 1028 2301
Fragments arranged by size:
2301 1028 761 628 275 186
BsiHKAI G_wGCw'C
Cuts at: 2433 3240 3325 4396 2433
Size: 807 85 1071 3216
Fragments arranged by size:
3216 1071 807 85
BsmI GAATG_Cn'
Cuts at: 2690 2783 2690
Size: 93 5086
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2165 2433 2498 3240 3325 4396 1391
Size: 399 375 268 65 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 375 268 85 65
BspEI T'CCGG_A
Cuts at: 4842 4842
Size: 5179
BspHI T'CATG_A
Cuts at: 3068 4798 3068
Size: 1730 3449
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2534 844
Size: 1690 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3066 1394
Size: 429 1243 3507
Fragments arranged by size:
3507 1243 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3674 3848 65
Size: 1183 2426 174 1396
Fragments arranged by size:
2426 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2397 2450 3823 4010 1700
Size: 110 587 53 1373 187 2869
Fragments arranged by size:
2869 1373 587 187 110 53
BsrGI T'GTAC_A
Cuts at: 96 2335 96
Size: 2239 2940
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5179
BssSI C'ACGA_G
Cuts at: 3239 4251 3239
Size: 1012 4167
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5179
BstBI TT'CG_AA
Cuts at: 3983 3983
Size: 5179
BstEII G'GTnAC_C
Cuts at: 1184 2233 2437 1184
Size: 1049 204 3926
Fragments arranged by size:
3926 1049 204
BstYI r'GATC_y
Cuts at: 1972 2217 2509 2876 3261 3278 4730 4816 4828
Size: 245 292 367 385 17 1452 86 12
Cuts at: 4828 5174 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 292 245 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2105 513
Size: 552 939 101 3587
Fragments arranged by size:
3587 939 552 101
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4440
Fragments arranged by size:
4440 683 56
BtsI GCAGTG_nn'
Cuts at: 2695 3489 3509 2695
Size: 794 20 4365
Fragments arranged by size:
4365 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5179
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5144
DraI TTT'AAA
Cuts at: 2483 2483
Size: 5179
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4186 809
Size: 507 2870 1802
Fragments arranged by size:
2870 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2626 3515 5144
Size: 54 1425 50 275 18 796 889 1629
Cuts at: 5144 8
Size: 43
Fragments arranged by size:
1629 1425 889 796 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2626 1537
Size: 275 814 4090
Fragments arranged by size:
4090 814 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2060 3109 1353
Size: 707 1049 3423
Fragments arranged by size:
3423 1049 707
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2230 2258 3766 4140 4286
Size: 1064 138 435 206 28 1508 374 146
Cuts at: 4286 387
Size: 1280
Fragments arranged by size:
1508 1280 1064 435 374 206 146 138
28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2338 3242 3839 4626 4894 1239
Size: 393 706 904 597 787 268 1524
Fragments arranged by size:
1524 904 787 706 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2353 2353
Size: 5179
EcoO109I rG'GnC_Cy
Cuts at: 2071 2071
Size: 5179
EcoRI G'AATT_C
Cuts at: 2488 2866 2488
Size: 378 4801
FspI TGC'GCA
Cuts at: 3685 3685
Size: 5179
HaeII r_GCGC'y
Cuts at: 1678 2320 4326 1678
Size: 642 2006 2531
Fragments arranged by size:
2531 2006 642
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2202 2234 3820 3852 3894
Size: 32 135 32 630 32 1586 32 42
Cuts at: 3894 3926 1373
Size: 32 2626
Fragments arranged by size:
2626 1586 630 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2523 2769 669
Size: 988 281 585 246 3079
Fragments arranged by size:
3079 988 585 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5179
HpaI GTT'AAC
Cuts at: 2769 2769
Size: 5179
KpnI G_GTAC'C
Cuts at: 2504 2504
Size: 5179
MfeI C'AATT_G
Cuts at: 2778 2778
Size: 5179
MluI A'CGCG_T
Cuts at: 5055 5055
Size: 5179
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4293 4477 1305
Size: 2988 184 2007
Fragments arranged by size:
2988 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3357
Fragments arranged by size:
3357 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3137 3496 3655 518
Size: 648 1971 359 159 2042
Fragments arranged by size:
2042 1971 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2107 2595 3272 4420 4665 1606
Size: 501 488 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 501 488 245
NaeI GCC'GGC
Cuts at: 2399 2452 2399
Size: 53 5126
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3688
Fragments arranged by size:
3688 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5179
NgoMIV G'CCGG_C
Cuts at: 2397 2450 2397
Size: 53 5126
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5179
NotI GC'GGCC_GC
Cuts at: 2626 2626
Size: 5179
NspI r_CATG'y
Cuts at: 1132 1229 2332 2537 4082 1132
Size: 97 1103 205 1545 2229
Fragments arranged by size:
2229 1545 1103 205 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5179
PciI A'CATG_T
Cuts at: 2328 4078 2328
Size: 1750 3429
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5179
PmeI GTTT'AAAC
Cuts at: 2483 2483
Size: 5179
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3248 3280 4761 4793 3248
Size: 32 1481 32 3634
Fragments arranged by size:
3634 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2071 2071
Size: 5179
PshAI GACnn'nnGTC
Cuts at: 1613 2261 1613
Size: 648 4531
PsiI TTA'TAA
Cuts at: 2749 2749
Size: 5179
PstI C_TGCA'G
Cuts at: 830 2531 830
Size: 1701 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5179
SacII CC_GC'GG
Cuts at: 2108 2108
Size: 5179
SalI G'TCGA_C
Cuts at: 1936 2521 1936
Size: 585 4594
SbfI CC_TGCA'GG
Cuts at: 2531 2531
Size: 5179
ScaI AGT'ACT
Cuts at: 3427 5139 3427
Size: 1712 3467
SfcI C'TryA_G
Cuts at: 826 1045 2527 3662 4343 4534 826
Size: 219 1482 1135 681 191 1471
Fragments arranged by size:
1482 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5179
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5179
SmaI CCC'GGG
Cuts at: 1872 2506 1872
Size: 634 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2432 3283 4184 4446 820
Size: 197 926 489 851 901 262 1553
Fragments arranged by size:
1553 926 901 851 489 262 197
SnaBI TAC'GTA
Cuts at: 493 2970 493
Size: 2477 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5179
SphI G_CATG'C
Cuts at: 1132 2537 1132
Size: 1405 3774
SspI AAT'ATT
Cuts at: 5 52 3103 5
Size: 47 3051 2081
Fragments arranged by size:
3051 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5179
StyI C'CwwG_G
Cuts at: 513 1065 2004 2145 2442 2618 513
Size: 552 939 141 297 176 3074
Fragments arranged by size:
3074 939 552 297 176 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2114 2158 3206 3223 3376 3561 4006
Size: 1255 534 44 1048 17 153 185 445
Cuts at: 4006 325
Size: 1498
Fragments arranged by size:
1498 1255 1048 534 445 185 153 44
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2298 2335 3425
Size: 274 80 33 51 1443 321 37 1090
Cuts at: 3425 5137 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1090 321 274 138 80 51
37 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5139 5171 5139
Size: 32 5147
Tth111I GACn'n_nGTC
Cuts at: 1316 2378 1316
Size: 1062 4117
XbaI T'CTAG_A
Cuts at: 2515 2515
Size: 5179
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5179
XmaI C'CCGG_G
Cuts at: 1870 2504 1870
Size: 634 4545
XmnI GAAnn'nnTTC
Cuts at: 2465 3308 2465
Size: 843 4336
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2362 2987 276
Size: 53 83 186 936 828 625 2468
Fragments arranged by size:
2468 936 828 625 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI