(Circular) (Six-base) MAPSORT of: pFN21AE1370.seq Check: 3082 from: 1 to: 5452 pFN21AE1370 5452 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3262 278 Size: 53 83 186 936 1726 2468 Fragments arranged by size: 2468 1726 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2795 1937 Size: 858 4594 Acc65I G'GTAC_C Cuts at: 2773 2773 Size: 5452 AclI AA'CG_TT Cuts at: 1563 3579 3952 1563 Size: 2016 373 3063 Fragments arranged by size: 3063 2016 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2603 3515 4899 5167 1632 Size: 971 912 1384 268 1917 Fragments arranged by size: 1917 1384 971 912 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5255 AflIII A'CryG_T Cuts at: 1203 4351 5328 1203 Size: 3148 977 1327 Fragments arranged by size: 3148 1327 977 AgeI A'CCGG_T Cuts at: 4283 4283 Size: 5452 AhdI GACnn_n'nnGTC Cuts at: 4181 4181 Size: 5452 AleI CACnn'nnGTG Cuts at: 1166 2120 2276 1166 Size: 954 156 4342 Fragments arranged by size: 4342 954 156 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5452 AlwNI CAG_nnn'CTG Cuts at: 2068 3195 4767 2068 Size: 1127 1572 2753 Fragments arranged by size: 2753 1572 1127 ApaLI G'TGCA_C Cuts at: 3509 4665 3509 Size: 1156 4296 ApoI r'AATT_y Cuts at: 1493 2723 2761 2987 3139 5248 1493 Size: 1230 38 226 152 2109 1697 Fragments arranged by size: 2109 1697 1230 226 152 38 AseI AT'TA_AT Cuts at: 160 1032 4006 160 Size: 872 2974 1606 Fragments arranged by size: 2974 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5452 AvaI C'yCGr_G Cuts at: 1870 1943 2777 1870 Size: 73 834 4545 Fragments arranged by size: 4545 834 73 BamHI G'GATC_C Cuts at: 2782 3149 2782 Size: 367 5085 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 2104 2773 4228 618 Size: 325 217 627 221 96 669 1455 1842 Fragments arranged by size: 1842 1455 669 627 325 221 217 96 BanII G_rGCy'C Cuts at: 1391 2079 2771 1391 Size: 688 692 4072 Fragments arranged by size: 4072 692 688 BbeI G_GCGC'C Cuts at: 2012 2012 Size: 5452 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4481 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3643 3677 1903 Size: 34 1706 34 3678 Fragments arranged by size: 3678 1706 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3344 4554 3344 Size: 1210 4242 BclI T'GATC_A Cuts at: 1548 2253 1548 Size: 705 4747 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4063 136 Size: 107 122 71 631 605 2391 1525 Fragments arranged by size: 2391 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5447 5447 Size: 5452 BlpI GC'TnA_GC Cuts at: 2869 2869 Size: 5452 Bme1580I G_kGCm'C Cuts at: 1790 2292 3513 4669 1790 Size: 502 1221 1156 2573 Fragments arranged by size: 2573 1221 1156 502 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5452 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2241 2655 3183 Size: 416 53 83 399 24 824 414 528 Cuts at: 3183 4141 442 Size: 958 1753 Fragments arranged by size: 1753 958 824 528 416 414 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5452 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1999 2497 4112 1239 Size: 760 498 1615 2579 Fragments arranged by size: 2579 1615 760 498 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2699 3577 4442 4740 2699 Size: 878 865 298 3411 Fragments arranged by size: 3411 878 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4115 882 Size: 617 2616 2219 Fragments arranged by size: 2616 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2274 3243 493 Size: 711 1070 969 2702 Fragments arranged by size: 2702 1070 969 711 BsaBI GATnn'nnATC Cuts at: 5446 5446 Size: 5452 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 3259 3641 4276 Size: 53 83 186 936 476 1250 382 635 Cuts at: 4276 275 Size: 1451 Fragments arranged by size: 1451 1250 936 635 476 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3885 4283 4557 4704 5115 3885 Size: 398 274 147 411 4222 Fragments arranged by size: 4222 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2625 2655 1178 Size: 30 165 30 1222 30 3975 Fragments arranged by size: 3975 1222 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5452 BseYI C'CCAG_C Cuts at: 4655 5151 4655 Size: 496 4956 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4879 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2112 2902 3663 4691 1540 Size: 275 186 111 790 761 1028 2301 Fragments arranged by size: 2301 1028 790 761 275 186 111 BsiHKAI G_wGCw'C Cuts at: 2079 2134 2618 3513 3598 4669 2079 Size: 55 484 895 85 1071 2862 Fragments arranged by size: 2862 1071 895 484 85 55 BsmI GAATG_Cn' Cuts at: 2963 3056 2963 Size: 93 5359 BsmBI CGTCTCn'nnnn_ Cuts at: 2599 2599 Size: 5452 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 2134 2292 2618 2771 3513 3598 Size: 399 289 55 158 326 153 742 85 Cuts at: 3598 4669 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 326 289 158 153 85 55 BspEI T'CCGG_A Cuts at: 5115 5115 Size: 5452 BspHI T'CATG_A Cuts at: 3341 5071 3341 Size: 1730 3722 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2663 2807 844 Size: 1819 144 3489 Fragments arranged by size: 3489 1819 144 BsrBI CCG'CTC Cuts at: 1394 1823 3339 1394 Size: 429 1516 3507 Fragments arranged by size: 3507 1516 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3947 4121 65 Size: 1183 2699 174 1396 Fragments arranged by size: 2699 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2107 4096 4283 1700 Size: 110 297 1989 187 2869 Fragments arranged by size: 2869 1989 297 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5452 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5452 BssSI C'ACGA_G Cuts at: 2561 3512 4524 2561 Size: 951 1012 3489 Fragments arranged by size: 3489 1012 951 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5452 BstBI TT'CG_AA Cuts at: 4256 4256 Size: 5452 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5452 BstXI CCAn_nnnn'nTGG Cuts at: 2278 2278 Size: 5452 BstYI r'GATC_y Cuts at: 1972 2173 2782 3149 3534 3551 5003 5089 5101 Size: 201 609 367 385 17 1452 86 12 Cuts at: 5101 5447 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 609 385 367 346 201 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3961 Fragments arranged by size: 3961 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2440 487 Size: 683 56 1214 3499 Fragments arranged by size: 3499 1214 683 56 BtsI GCAGTG_nn' Cuts at: 2968 3762 3782 2968 Size: 794 20 4638 Fragments arranged by size: 4638 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5452 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5417 DraI TTT'AAA Cuts at: 2756 2756 Size: 5452 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4459 809 Size: 507 3143 1802 Fragments arranged by size: 3143 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2097 2109 2667 Size: 54 1425 50 275 18 267 12 558 Cuts at: 2667 2899 3788 5417 8 Size: 232 889 1629 43 Fragments arranged by size: 1629 1425 889 558 275 267 232 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2109 2899 1537 Size: 275 297 790 4090 Fragments arranged by size: 4090 790 297 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3382 1353 Size: 2029 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2071 4039 4413 4559 387 Size: 1064 138 482 1968 374 146 1280 Fragments arranged by size: 1968 1280 1064 482 374 146 138 EcoICRI GAG'CTC Cuts at: 2077 2077 Size: 5452 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1999 2497 2603 3515 4112 4899 5167 Size: 393 367 498 106 912 597 787 268 Cuts at: 5167 1239 Size: 1524 Fragments arranged by size: 1524 912 787 597 498 393 367 268 106 EcoRI G'AATT_C Cuts at: 2723 2761 3139 2723 Size: 38 378 5036 Fragments arranged by size: 5036 378 38 FspI TGC'GCA Cuts at: 2196 3958 2196 Size: 1762 3690 HaeII r_GCGC'y Cuts at: 1678 2012 4599 1678 Size: 334 2587 2531 Fragments arranged by size: 2587 2531 334 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4093 4125 4167 4199 1373 Size: 32 135 32 2521 32 42 32 2626 Fragments arranged by size: 2626 2521 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2796 3042 669 Size: 988 281 858 246 3079 Fragments arranged by size: 3079 988 858 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5452 HpaI GTT'AAC Cuts at: 3042 3042 Size: 5452 KasI G'GCGC_C Cuts at: 2008 2008 Size: 5452 KpnI G_GTAC'C Cuts at: 2777 2777 Size: 5452 MfeI C'AATT_G Cuts at: 3051 3051 Size: 5452 MluI A'CGCG_T Cuts at: 5328 5328 Size: 5452 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2613 4566 4750 1305 Size: 1308 1953 184 2007 Fragments arranged by size: 2007 1953 1308 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2669 10 Size: 54 1425 343 837 2793 Fragments arranged by size: 2793 1425 837 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2120 2276 3410 3769 3928 518 Size: 648 954 156 1134 359 159 2042 Fragments arranged by size: 2042 1134 954 648 359 159 156 MspA1I CmG'CkG Cuts at: 1606 2868 3545 4693 4938 1606 Size: 1262 677 1148 245 2120 Fragments arranged by size: 2120 1262 1148 677 245 NaeI GCC'GGC Cuts at: 2109 2109 Size: 5452 NarI GG'CG_CC Cuts at: 2009 2009 Size: 5452 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3961 Fragments arranged by size: 3961 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5452 NgoMIV G'CCGG_C Cuts at: 2107 2107 Size: 5452 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5452 NotI GC'GGCC_GC Cuts at: 2899 2899 Size: 5452 NspI r_CATG'y Cuts at: 1132 1229 2309 2810 4355 1132 Size: 97 1080 501 1545 2229 Fragments arranged by size: 2229 1545 1080 501 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5452 PciI A'CATG_T Cuts at: 4351 4351 Size: 5452 PflMI CCAn_nnn'nTGG Cuts at: 1108 2356 1108 Size: 1248 4204 PfoI T'CCnGG_A Cuts at: 2015 2015 Size: 5452 PmeI GTTT'AAAC Cuts at: 2756 2756 Size: 5452 PmlI CAC'GTG Cuts at: 2274 2274 Size: 5452 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3521 3553 5034 5066 3521 Size: 32 1481 32 3907 Fragments arranged by size: 3907 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2450 1613 Size: 837 4615 PsiI TTA'TAA Cuts at: 3022 3022 Size: 5452 PstI C_TGCA'G Cuts at: 830 2804 830 Size: 1974 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5452 SacI G_AGCT'C Cuts at: 2079 2079 Size: 5452 SalI G'TCGA_C Cuts at: 1936 2794 1936 Size: 858 4594 SbfI CC_TGCA'GG Cuts at: 2804 2804 Size: 5452 ScaI AGT'ACT Cuts at: 3700 5412 3700 Size: 1712 3740 SfcI C'TryA_G Cuts at: 826 1045 2800 3935 4616 4807 826 Size: 219 1755 1135 681 191 1471 Fragments arranged by size: 1755 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2010 2010 Size: 5452 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5452 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5452 SmaI CCC'GGG Cuts at: 1872 2779 1872 Size: 907 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2714 3556 4457 4719 820 Size: 197 926 771 842 901 262 1553 Fragments arranged by size: 1553 926 901 842 771 262 197 SnaBI TAC'GTA Cuts at: 493 3243 493 Size: 2750 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5452 SphI G_CATG'C Cuts at: 1132 2810 1132 Size: 1678 3774 SspI AAT'ATT Cuts at: 5 52 3376 5 Size: 47 3324 2081 Fragments arranged by size: 3324 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5452 StyI C'CwwG_G Cuts at: 513 1065 2004 2100 2891 513 Size: 552 939 96 791 3074 Fragments arranged by size: 3074 939 791 552 96 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2266 2690 3479 3496 3649 3834 4279 Size: 1255 686 424 789 17 153 185 445 Cuts at: 4279 325 Size: 1498 Fragments arranged by size: 1498 1255 789 686 445 424 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2557 3698 5410 Size: 274 80 33 51 1443 580 1141 1712 Cuts at: 5410 96 Size: 138 Fragments arranged by size: 1712 1443 1141 580 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2625 2657 5412 5444 2625 Size: 32 2755 32 2633 Fragments arranged by size: 2755 2633 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5452 XbaI T'CTAG_A Cuts at: 2788 2788 Size: 5452 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2185 2185 Size: 5452 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5452 XmaI C'CCGG_G Cuts at: 1870 2777 1870 Size: 907 4545 XmnI GAAnn'nnTTC Cuts at: 2024 3581 2024 Size: 1557 3895 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3260 276 Size: 53 83 186 936 1726 2468 Fragments arranged by size: 2468 1726 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI