(Circular) (Six-base) MAPSORT of: pFN21AB6664.seq Check: 122 from: 1 to: 6061 pFN21AB6664 6061 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3871 278 Size: 53 83 186 936 2335 2468 Fragments arranged by size: 2468 2335 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3404 1937 Size: 1467 4594 Acc65I G'GTAC_C Cuts at: 3382 3382 Size: 6061 AclI AA'CG_TT Cuts at: 1563 2190 4188 4561 1563 Size: 627 1998 373 3063 Fragments arranged by size: 3063 1998 627 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2463 4124 5508 5776 1632 Size: 831 1661 1384 268 1917 Fragments arranged by size: 1917 1661 1384 831 268 AflII C'TTAA_G Cuts at: 820 1017 2168 820 Size: 197 1151 4713 Fragments arranged by size: 4713 1151 197 AflIII A'CryG_T Cuts at: 1203 4960 5937 1203 Size: 3757 977 1327 Fragments arranged by size: 3757 1327 977 AgeI A'CCGG_T Cuts at: 4892 4892 Size: 6061 AhdI GACnn_n'nnGTC Cuts at: 4790 4790 Size: 6061 AleI CACnn'nnGTG Cuts at: 1166 2879 1166 Size: 1713 4348 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6061 AlwNI CAG_nnn'CTG Cuts at: 2513 2770 3221 3804 5376 2513 Size: 257 451 583 1572 3198 Fragments arranged by size: 3198 1572 583 451 257 ApaLI G'TGCA_C Cuts at: 4118 5274 4118 Size: 1156 4905 ApoI r'AATT_y Cuts at: 1493 2077 2365 2545 2640 3370 3596 3748 5857 Size: 584 288 180 95 730 226 152 2109 Cuts at: 5857 1493 Size: 1697 Fragments arranged by size: 2109 1697 730 584 288 226 180 152 95 AseI AT'TA_AT Cuts at: 160 1032 2133 4615 160 Size: 872 1101 2482 1606 Fragments arranged by size: 2482 1606 1101 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 AvaI C'yCGr_G Cuts at: 1870 1943 2032 3386 1870 Size: 73 89 1354 4545 Fragments arranged by size: 4545 1354 89 73 AvrII C'CTAG_G Cuts at: 2572 2572 Size: 6061 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2814 2847 2814 Size: 33 6028 BamHI G'GATC_C Cuts at: 3391 3758 3391 Size: 367 5694 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2042 3382 4837 618 Size: 325 217 627 255 1340 1455 1842 Fragments arranged by size: 1842 1455 1340 627 325 255 217 BanII G_rGCy'C Cuts at: 1391 2012 3128 3380 1391 Size: 621 1116 252 4072 Fragments arranged by size: 4072 1116 621 252 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3150 928 Size: 971 1251 3839 Fragments arranged by size: 3839 1251 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4252 4286 1903 Size: 34 2315 34 3678 Fragments arranged by size: 3678 2315 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3953 5163 3953 Size: 1210 4851 BclI T'GATC_A Cuts at: 1548 1548 Size: 6061 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4672 136 Size: 107 122 71 631 605 3000 1525 Fragments arranged by size: 3000 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2446 6056 2446 Size: 3610 2451 BlpI GC'TnA_GC Cuts at: 3478 3478 Size: 6061 Bme1580I G_kGCm'C Cuts at: 1790 2045 4122 5278 1790 Size: 255 2077 1156 2573 Fragments arranged by size: 2573 2077 1156 255 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6061 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3299 3792 4750 Size: 416 53 83 399 24 1882 493 958 Cuts at: 4750 442 Size: 1753 Fragments arranged by size: 1882 1753 958 493 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6061 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2372 4721 1239 Size: 1133 2349 2579 Fragments arranged by size: 2579 2349 1133 Bpu10I CC'TnA_GC Cuts at: 3142 3142 Size: 6061 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4186 5051 5349 4186 Size: 865 298 4898 Fragments arranged by size: 4898 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2240 4724 882 Size: 617 741 2484 2219 Fragments arranged by size: 2484 2219 741 617 BsaAI yAC'GTr Cuts at: 493 1204 3852 493 Size: 711 2648 2702 Fragments arranged by size: 2702 2648 711 BsaBI GATnn'nnATC Cuts at: 2213 6055 2213 Size: 3842 2219 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3248 3868 4250 4885 Size: 53 83 186 936 1715 620 382 635 Cuts at: 4885 275 Size: 1451 Fragments arranged by size: 1715 1451 936 635 620 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4494 4892 5166 5313 5724 4494 Size: 398 274 147 411 4831 Fragments arranged by size: 4831 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3209 3239 1178 Size: 30 165 30 1806 30 4000 Fragments arranged by size: 4000 1806 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2002 3217 1186 Size: 816 1215 4030 Fragments arranged by size: 4030 1215 816 BseYI C'CCAG_C Cuts at: 2971 5264 5760 2971 Size: 2293 496 3272 Fragments arranged by size: 3272 2293 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5488 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3511 4272 5300 1540 Size: 275 186 1510 761 1028 2301 Fragments arranged by size: 2301 1510 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3128 4122 4207 5278 3128 Size: 994 85 1071 3911 Fragments arranged by size: 3911 1071 994 85 BsmI GAATG_Cn' Cuts at: 2301 3572 3665 2301 Size: 1271 93 4697 Fragments arranged by size: 4697 1271 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2045 3128 3380 4122 4207 5278 Size: 399 222 33 1083 252 742 85 1071 Cuts at: 5278 1391 Size: 2174 Fragments arranged by size: 2174 1083 1071 742 399 252 222 85 33 BspEI T'CCGG_A Cuts at: 5724 5724 Size: 6061 BspHI T'CATG_A Cuts at: 2430 3950 5680 2430 Size: 1520 1730 2811 Fragments arranged by size: 2811 1730 1520 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2375 3416 844 Size: 1531 1041 3489 Fragments arranged by size: 3489 1531 1041 BsrBI CCG'CTC Cuts at: 1394 1823 3948 1394 Size: 429 2125 3507 Fragments arranged by size: 3507 2125 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3066 4556 4730 65 Size: 1183 1818 1490 174 1396 Fragments arranged by size: 1818 1490 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4705 4892 1700 Size: 110 2895 187 2869 Fragments arranged by size: 2895 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2499 96 Size: 2403 3658 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6061 BssSI C'ACGA_G Cuts at: 4121 5133 4121 Size: 1012 5049 BstAPI GCAn_nnn'nTGC Cuts at: 794 3221 794 Size: 2427 3634 BstBI TT'CG_AA Cuts at: 4865 4865 Size: 6061 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6061 BstYI r'GATC_y Cuts at: 1972 2028 2173 2446 3391 3758 4143 4160 5612 Size: 56 145 273 945 367 385 17 1452 Cuts at: 5612 5698 5710 6056 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 945 385 367 346 273 145 86 56 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4570 Fragments arranged by size: 4570 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5322 Fragments arranged by size: 5322 683 56 BtsI GCAGTG_nn' Cuts at: 3255 3577 4371 4391 3255 Size: 322 794 20 4925 Fragments arranged by size: 4925 794 322 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6061 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3259 3294 529 Size: 35 2695 35 3296 Fragments arranged by size: 3296 2695 35 35 DraI TTT'AAA Cuts at: 3365 3365 Size: 6061 DraIII CAC_nnn'GTG Cuts at: 2402 2402 Size: 6061 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5068 809 Size: 507 3752 1802 Fragments arranged by size: 3752 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2278 3508 4397 Size: 54 1425 50 275 18 448 1230 889 Cuts at: 4397 6026 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1230 889 448 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3508 1537 Size: 275 1696 4090 Fragments arranged by size: 4090 1696 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2652 3039 3133 3991 1353 Size: 1299 387 94 858 3423 Fragments arranged by size: 3423 1299 858 387 94 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4648 5022 5168 387 Size: 1064 138 3059 374 146 1280 Fragments arranged by size: 3059 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3126 3126 Size: 6061 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2372 2463 4124 4721 5508 5776 1239 Size: 393 740 91 1661 597 787 268 1524 Fragments arranged by size: 1661 1524 787 740 597 393 268 91 EcoO109I rG'GnC_Cy Cuts at: 3265 3265 Size: 6061 EcoRI G'AATT_C Cuts at: 3370 3748 3370 Size: 378 5683 FspI TGC'GCA Cuts at: 4567 4567 Size: 6061 HaeII r_GCGC'y Cuts at: 1678 5208 1678 Size: 3530 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2345 2377 3140 3172 3209 Size: 32 135 32 773 32 763 32 37 Cuts at: 3209 3241 4702 4734 4776 4808 1373 Size: 32 1461 32 42 32 2626 Fragments arranged by size: 2626 1461 773 763 135 42 37 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3405 3651 669 Size: 988 281 1467 246 3079 Fragments arranged by size: 3079 1467 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6061 HpaI GTT'AAC Cuts at: 3651 3651 Size: 6061 KpnI G_GTAC'C Cuts at: 3386 3386 Size: 6061 MfeI C'AATT_G Cuts at: 3660 3660 Size: 6061 MluI A'CGCG_T Cuts at: 5937 5937 Size: 6061 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5175 5359 1305 Size: 3870 184 2007 Fragments arranged by size: 3870 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4239 Fragments arranged by size: 4239 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2879 4019 4378 4537 518 Size: 648 1713 1140 359 159 2042 Fragments arranged by size: 2042 1713 1140 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2710 2804 3477 4154 5302 5547 1606 Size: 1104 94 673 677 1148 245 2120 Fragments arranged by size: 2120 1148 1104 677 673 245 94 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4570 Fragments arranged by size: 4570 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6061 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6061 NotI GC'GGCC_GC Cuts at: 3508 3508 Size: 6061 NspI r_CATG'y Cuts at: 1132 1229 2348 3419 4964 1132 Size: 97 1119 1071 1545 2229 Fragments arranged by size: 2229 1545 1119 1071 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6061 PciI A'CATG_T Cuts at: 4960 4960 Size: 6061 PflMI CCAn_nnn'nTGG Cuts at: 1108 2996 1108 Size: 1888 4173 PfoI T'CCnGG_A Cuts at: 2650 2650 Size: 6061 PmeI GTTT'AAAC Cuts at: 3365 3365 Size: 6061 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4130 4162 5643 5675 4130 Size: 32 1481 32 4516 Fragments arranged by size: 4516 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2252 1613 Size: 639 5422 PsiI TTA'TAA Cuts at: 3631 3631 Size: 6061 PstI C_TGCA'G Cuts at: 830 2315 2377 3136 3413 830 Size: 1485 62 759 277 3478 Fragments arranged by size: 3478 1485 759 277 62 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6061 PvuII CAG'CTG Cuts at: 2710 2804 2710 Size: 94 5967 SacI G_AGCT'C Cuts at: 3128 3128 Size: 6061 SalI G'TCGA_C Cuts at: 1936 3403 1936 Size: 1467 4594 SapI GCTCTTCn'nnn_ Cuts at: 3133 3133 Size: 6061 SbfI CC_TGCA'GG Cuts at: 3413 3413 Size: 6061 ScaI AGT'ACT Cuts at: 4309 6021 4309 Size: 1712 4349 SexAI A'CCwGG_T Cuts at: 2911 2911 Size: 6061 SfcI C'TryA_G Cuts at: 826 1045 2218 2311 2373 2751 3132 3409 4544 Size: 219 1173 93 62 378 381 277 1135 Cuts at: 4544 5225 5416 826 Size: 681 191 1471 Fragments arranged by size: 1471 1173 1135 681 381 378 277 219 191 93 62 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6061 SmaI CCC'GGG Cuts at: 1872 2034 3388 1872 Size: 162 1354 4545 Fragments arranged by size: 4545 1354 162 SmlI C'TyrA_G Cuts at: 820 1017 1943 2168 4165 5066 5328 820 Size: 197 926 225 1997 901 262 1553 Fragments arranged by size: 1997 1553 926 901 262 225 197 SnaBI TAC'GTA Cuts at: 493 3852 493 Size: 3359 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6061 SphI G_CATG'C Cuts at: 1132 3419 1132 Size: 2287 3774 SspI AAT'ATT Cuts at: 5 52 3985 5 Size: 47 3933 2081 Fragments arranged by size: 3933 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6061 StyI C'CwwG_G Cuts at: 513 1065 2004 2572 3111 3261 3500 513 Size: 552 939 568 539 150 239 3074 Fragments arranged by size: 3074 939 568 552 539 239 150 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4088 4105 4258 4443 4888 325 Size: 1255 2508 17 153 185 445 1498 Fragments arranged by size: 2508 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2499 2822 4307 Size: 274 80 33 51 1443 522 323 1485 Cuts at: 4307 6019 96 Size: 1712 138 Fragments arranged by size: 1712 1485 1443 522 323 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6021 6053 6021 Size: 32 6029 Tth111I GACn'n_nGTC Cuts at: 1316 3287 1316 Size: 1971 4090 XbaI T'CTAG_A Cuts at: 3397 3397 Size: 6061 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2268 2550 2268 Size: 282 5779 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6061 XmaI C'CCGG_G Cuts at: 1870 2032 3386 1870 Size: 162 1354 4545 Fragments arranged by size: 4545 1354 162 XmnI GAAnn'nnTTC Cuts at: 4190 4190 Size: 6061 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3869 276 Size: 53 83 186 936 2335 2468 Fragments arranged by size: 2468 2335 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI