(Circular) (Six-base) MAPSORT of: pFN21AB5758.seq Check: 3418 from: 1 to: 6922 pFN21AB5758 6922 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4732 278 Size: 53 83 186 936 3196 2468 Fragments arranged by size: 3196 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2271 2277 2361 2589 3761 3945 4265 1937 Size: 334 6 84 228 1172 184 320 4594 Fragments arranged by size: 4594 1172 334 320 228 184 84 6 Acc65I G'GTAC_C Cuts at: 4243 4243 Size: 6922 AclI AA'CG_TT Cuts at: 1563 5049 5422 1563 Size: 3486 373 3063 Fragments arranged by size: 3486 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2408 3657 3802 4985 6369 6637 1632 Size: 776 1249 145 1183 1384 268 1917 Fragments arranged by size: 1917 1384 1249 1183 776 268 145 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6725 AflIII A'CryG_T Cuts at: 1203 5821 6798 1203 Size: 4618 977 1327 Fragments arranged by size: 4618 1327 977 AgeI A'CCGG_T Cuts at: 5753 5753 Size: 6922 AhdI GACnn_n'nnGTC Cuts at: 5651 5651 Size: 6922 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6922 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2781 1184 Size: 1597 5325 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3765 3797 3765 Size: 32 6890 AlwNI CAG_nnn'CTG Cuts at: 2291 2530 2903 4665 6237 2291 Size: 239 373 1762 1572 2976 Fragments arranged by size: 2976 1762 1572 373 239 ApaLI G'TGCA_C Cuts at: 4979 6135 4979 Size: 1156 5766 ApoI r'AATT_y Cuts at: 1493 2594 2923 2986 3038 3620 4231 4457 4609 Size: 1101 329 63 52 582 611 226 152 Cuts at: 4609 6718 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1101 611 582 329 226 152 63 52 AseI AT'TA_AT Cuts at: 160 1032 3207 5476 160 Size: 872 2175 2269 1606 Fragments arranged by size: 2269 2175 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6922 AvaI C'yCGr_G Cuts at: 1870 1943 4247 1870 Size: 73 2304 4545 Fragments arranged by size: 4545 2304 73 BamHI G'GATC_C Cuts at: 4252 4619 4252 Size: 367 6555 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2197 4243 5698 618 Size: 325 217 627 410 2046 1455 1842 Fragments arranged by size: 2046 1842 1455 627 410 325 217 BanII G_rGCy'C Cuts at: 1391 2298 4241 1391 Size: 907 1943 4072 Fragments arranged by size: 4072 1943 907 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5951 BbvCI CC'TCA_GC Cuts at: 2111 2184 2111 Size: 73 6849 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2122 2156 5113 5147 1903 Size: 34 50 34 101 34 2957 34 3678 Fragments arranged by size: 3678 2957 101 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4182 4814 6024 4182 Size: 632 1210 5080 Fragments arranged by size: 5080 1210 632 BclI T'GATC_A Cuts at: 1548 2407 3024 3669 1548 Size: 859 617 645 4801 Fragments arranged by size: 4801 859 645 617 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2071 5533 136 Size: 107 122 71 631 605 399 3462 1525 Fragments arranged by size: 3462 1525 631 605 399 122 107 71 BglII A'GATC_T Cuts at: 2230 3003 4098 6917 2230 Size: 773 1095 2819 2235 Fragments arranged by size: 2819 2235 1095 773 BlpI GC'TnA_GC Cuts at: 2292 4339 2292 Size: 2047 4875 Bme1580I G_kGCm'C Cuts at: 1790 4983 6139 1790 Size: 3193 1156 2573 Fragments arranged by size: 3193 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6922 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3069 3495 4653 Size: 416 53 83 399 24 1652 426 1158 Cuts at: 4653 5611 442 Size: 958 1753 Fragments arranged by size: 1753 1652 1158 958 426 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2213 1056 Size: 1157 5765 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2518 2552 2811 2889 3258 5582 1239 Size: 1279 34 259 78 369 2324 2579 Fragments arranged by size: 2579 2324 1279 369 259 78 34 Bpu10I CC'TnA_GC Cuts at: 2111 2184 2559 2111 Size: 73 375 6474 Fragments arranged by size: 6474 375 73 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2911 5047 5912 6210 2911 Size: 2136 865 298 3623 Fragments arranged by size: 3623 2136 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2887 5585 882 Size: 617 1388 2698 2219 Fragments arranged by size: 2698 2219 1388 617 BsaAI yAC'GTr Cuts at: 493 1204 4713 493 Size: 711 3509 2702 Fragments arranged by size: 3509 2702 711 BsaBI GATnn'nnATC Cuts at: 6916 6916 Size: 6922 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4729 5111 5746 275 Size: 53 83 186 936 3196 382 635 1451 Fragments arranged by size: 3196 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5355 5753 6027 6174 6585 5355 Size: 398 274 147 411 5692 Fragments arranged by size: 5692 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2076 2106 2220 2250 2321 Size: 30 165 30 673 30 114 30 71 Cuts at: 2321 2351 3174 3204 4154 4184 1178 Size: 30 823 30 950 30 3916 Fragments arranged by size: 3916 950 823 673 165 114 71 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2201 2407 1186 Size: 1015 206 5701 Fragments arranged by size: 5701 1015 206 BseYI C'CCAG_C Cuts at: 2736 4112 6125 6621 2736 Size: 1376 2013 496 3037 Fragments arranged by size: 3037 2013 1376 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2889 1163 Size: 573 1153 5196 Fragments arranged by size: 5196 1153 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4372 5133 6161 1540 Size: 275 186 2371 761 1028 2301 Fragments arranged by size: 2371 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2041 2298 3063 4983 5068 6139 2041 Size: 257 765 1920 85 1071 2824 Fragments arranged by size: 2824 1920 1071 765 257 85 BsmI GAATG_Cn' Cuts at: 4433 4526 4433 Size: 93 6829 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2041 2298 3063 4241 4983 5068 6139 Size: 399 251 257 765 1178 742 85 1071 Cuts at: 6139 1391 Size: 2174 Fragments arranged by size: 2174 1178 1071 765 742 399 257 251 85 BspEI T'CCGG_A Cuts at: 6585 6585 Size: 6922 BspHI T'CATG_A Cuts at: 4811 6541 4811 Size: 1730 5192 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4277 844 Size: 3433 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2048 3164 4809 1394 Size: 429 225 1116 1645 3507 Fragments arranged by size: 3507 1645 1116 429 225 BsrDI GCAATG_nn' Cuts at: 65 1248 5417 5591 65 Size: 1183 4169 174 1396 Fragments arranged by size: 4169 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2103 5566 5753 1700 Size: 110 293 3463 187 2869 Fragments arranged by size: 3463 2869 293 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6922 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6922 BssSI C'ACGA_G Cuts at: 3423 4982 5994 3423 Size: 1559 1012 4351 Fragments arranged by size: 4351 1559 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6922 BstBI TT'CG_AA Cuts at: 5726 5726 Size: 6922 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6922 BstYI r'GATC_y Cuts at: 1972 2230 2526 2770 3003 3070 4098 4252 4619 Size: 258 296 244 233 67 1028 154 367 Cuts at: 4619 5004 5021 6473 6559 6571 6917 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1028 385 367 346 296 258 244 233 154 86 67 17 12 BstZ17I GTA'TAC Cuts at: 3762 3762 Size: 6922 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6370 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6183 Fragments arranged by size: 6183 683 56 BtsI GCAGTG_nn' Cuts at: 2220 2292 4050 4438 5232 5252 2220 Size: 72 1758 388 794 20 3890 Fragments arranged by size: 3890 1758 794 388 72 20 ClaI AT'CG_AT Cuts at: 1554 2538 1554 Size: 984 5938 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6887 DraI TTT'AAA Cuts at: 2571 4226 2571 Size: 1655 5267 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5929 809 Size: 507 4613 1802 Fragments arranged by size: 4613 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2581 3235 4369 Size: 54 1425 50 275 18 751 654 1134 Cuts at: 4369 5258 6887 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1134 889 751 654 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4369 1537 Size: 275 2557 4090 Fragments arranged by size: 4090 2557 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2384 3674 4852 1353 Size: 1031 1290 1178 3423 Fragments arranged by size: 3423 1290 1178 1031 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2090 5509 5883 6029 387 Size: 1064 138 501 3419 374 146 1280 Fragments arranged by size: 3419 1280 1064 501 374 146 138 EcoICRI GAG'CTC Cuts at: 2296 2296 Size: 6922 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2408 2518 2552 2811 2889 3258 3657 Size: 393 776 110 34 259 78 369 399 Cuts at: 3657 3802 4985 5582 6369 6637 1239 Size: 145 1183 597 787 268 1524 Fragments arranged by size: 1524 1183 787 776 597 399 393 369 268 259 145 110 78 34 EcoNI CCTnn'n_nnAGG Cuts at: 3257 3257 Size: 6922 EcoRI G'AATT_C Cuts at: 3038 3620 4231 4609 3038 Size: 582 611 378 5351 Fragments arranged by size: 5351 611 582 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3458 3458 Size: 6922 FspI TGC'GCA Cuts at: 5428 5428 Size: 6922 HaeII r_GCGC'y Cuts at: 1678 3976 6069 1678 Size: 2298 2093 2531 Fragments arranged by size: 2531 2298 2093 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2076 2108 2995 3027 3332 Size: 32 135 32 504 32 887 32 305 Cuts at: 3332 3364 3541 3573 3938 3970 4058 4083 4090 Size: 32 177 32 365 32 88 25 7 Cuts at: 4090 4115 5563 5595 5637 5669 1373 Size: 25 1448 32 42 32 2626 Fragments arranged by size: 2626 1448 887 504 365 305 177 135 88 42 32 32 32 32 32 32 32 32 32 25 25 7 HincII GTy'rAC Cuts at: 669 1657 1938 3657 4266 4512 669 Size: 988 281 1719 609 246 3079 Fragments arranged by size: 3079 1719 988 609 281 246 HindIII A'AGCT_T Cuts at: 748 2412 748 Size: 1664 5258 HpaI GTT'AAC Cuts at: 3657 4512 3657 Size: 855 6067 KpnI G_GTAC'C Cuts at: 4247 4247 Size: 6922 MfeI C'AATT_G Cuts at: 3513 3627 4521 3513 Size: 114 894 5914 Fragments arranged by size: 5914 894 114 MluI A'CGCG_T Cuts at: 6798 6798 Size: 6922 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3244 3347 3361 6036 6220 1305 Size: 1939 103 14 2675 184 2007 Fragments arranged by size: 2675 2007 1939 184 103 14 MscI TGG'CCA Cuts at: 10 64 1489 1832 2583 10 Size: 54 1425 343 751 4349 Fragments arranged by size: 4349 1425 751 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3847 4880 5239 5398 518 Size: 648 2681 1033 359 159 2042 Fragments arranged by size: 2681 2042 1033 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3153 4338 5015 6163 6408 1606 Size: 1547 1185 677 1148 245 2120 Fragments arranged by size: 2120 1547 1185 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6370 NdeI CA'TA_TG Cuts at: 387 387 Size: 6922 NheI G'CTAG_C Cuts at: 1052 2209 1052 Size: 1157 5765 NotI GC'GGCC_GC Cuts at: 4369 4369 Size: 6922 NspI r_CATG'y Cuts at: 1132 1229 2881 4280 5825 1132 Size: 97 1652 1399 1545 2229 Fragments arranged by size: 2229 1652 1545 1399 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6922 PciI A'CATG_T Cuts at: 5821 5821 Size: 6922 PflMI CCAn_nnn'nTGG Cuts at: 1108 3106 1108 Size: 1998 4924 PfoI T'CCnGG_A Cuts at: 2156 2529 2156 Size: 373 6549 PmeI GTTT'AAAC Cuts at: 4226 4226 Size: 6922 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4991 5023 6504 6536 4991 Size: 32 1481 32 5377 Fragments arranged by size: 5377 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6922 PsiI TTA'TAA Cuts at: 4492 4492 Size: 6922 PstI C_TGCA'G Cuts at: 830 4274 830 Size: 3444 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6922 SacI G_AGCT'C Cuts at: 2298 2298 Size: 6922 SalI G'TCGA_C Cuts at: 1936 4264 1936 Size: 2328 4594 SbfI CC_TGCA'GG Cuts at: 4274 4274 Size: 6922 ScaI AGT'ACT Cuts at: 5170 6882 5170 Size: 1712 5210 SfcI C'TryA_G Cuts at: 826 1045 3831 4270 5405 6086 6277 826 Size: 219 2786 439 1135 681 191 1471 Fragments arranged by size: 2786 1471 1135 681 439 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6922 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6922 SmaI CCC'GGG Cuts at: 1872 4249 1872 Size: 2377 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2890 5026 5927 6189 820 Size: 197 926 947 2136 901 262 1553 Fragments arranged by size: 2136 1553 947 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4713 493 Size: 4220 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6922 SphI G_CATG'C Cuts at: 1132 4280 1132 Size: 3148 3774 SspI AAT'ATT Cuts at: 5 52 2485 4846 5 Size: 47 2433 2361 2081 Fragments arranged by size: 2433 2361 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6922 StyI C'CwwG_G Cuts at: 513 1065 2950 4361 513 Size: 552 1885 1411 3074 Fragments arranged by size: 3074 1885 1411 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2379 4949 4966 5119 5304 5749 325 Size: 1255 799 2570 17 153 185 445 1498 Fragments arranged by size: 2570 1498 1255 799 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5168 6880 96 Size: 274 80 33 51 1443 3191 1712 138 Fragments arranged by size: 3191 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2220 2252 6882 6914 2220 Size: 32 4630 32 2228 Fragments arranged by size: 4630 2228 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6922 XbaI T'CTAG_A Cuts at: 4258 4258 Size: 6922 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6922 XmaI C'CCGG_G Cuts at: 1870 4247 1870 Size: 2377 4545 XmnI GAAnn'nnTTC Cuts at: 2637 3683 3714 5051 2637 Size: 1046 31 1337 4508 Fragments arranged by size: 4508 1337 1046 31 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4730 276 Size: 53 83 186 936 3196 2468 Fragments arranged by size: 3196 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI