Restriction map for
pFN21AE1041
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# NO CUT ENZYMES ( 165 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
Alw44I AlwI AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspHI AspI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BbvI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsoBI BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrI BssHII BssSI Bst1107I BstUI
BstXI BstYI BstZ17I Bsu36I Cac8I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII HphI KasI
KpnI KspI MamI MfeI MflI
MluI MroI MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI StyI
SwaI TseI Tth111I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AciI 775 GCGG CCGC(-3/-1)
AcsI 760 GAATTC [GA]^AATT[CT]
AhdI 745 GACTATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 350 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 309 GGGCCC GGGCC^C
ApoI 760 GAATTC [GA]^AATT[CT]
AspEI 745 GACTATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 526 GGTCC G^G[AT]CC
BalI 31 TGGCCA TGG^CCA
BanII 309 GGGCCC G[GA]GC[CT]^C
BbsI 869 GTCTTC GAAGAC(2/6)
BfaI 590 CTAG C^TAG
BpuAI 869 GTCTTC GAAGAC(2/6)
BsaAI 726 CACGTA [CT]AC^GT[GA]
BsmAI 795 GTCTC GTCTC(1/5)
BsmFI 536 GTCCC GGGAC(10/14)
BsmI 502 GAATGC GAATGC(1/-1)
Bsp1407I 549 TGTACA T^GTACA
BspMI 180 ACCTGC ACCTGC(4/8)
BsrGI 549 TGTACA T^GTACA
BstAPI 398 GCACAGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 584 TTCGAA TT^CGAA
BstEII 1039 GGTTACC G^GT[ACGT]ACC
BstPI 1039 GGTTACC G^GT[ACGT]ACC
DpnII 739 GATC ^GATC
DraII 308 AGGGCCC [GA]G^G[ACGT]CC[CT]
EaeI 31 TGGCCA [CT]^GGCC[GA]
Eam1105I 745 GACTATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 916 GAAGAG CTCTTC(1/4)
EcoO109I 308 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 1039 GGTTACC G^GT[ACGT]ACC
EcoRI 760 GAATTC G^AATTC
Fnu4HI 775 GCGGC GC^[ACGT]GC
ItaI 775 GCGGC GC^[ACGT]GC
Ksp632I 916 GAAGAG CTCTTC(1/4)
MaeI 590 CTAG C^TAG
MboI 739 GATC ^GATC
MluNI 31 TGGCCA TGG^CCA
MscI 31 TGGCCA TGG^CCA
MslI 811 CACAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 813 CAGCTG C[AC]G^C[GT]G
NdeII 739 GATC ^GATC
NspV 584 TTCGAA TT^CGAA
PflMI 349 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 751 GAGTC GAGTC(4/5)
PstI 229 CTGCAG CTGCA^G
PvuII 813 CAGCTG CAG^CTG
SapI 916 GAAGAGC GCTCTTC(1/4)
Sau3AI 739 GATC ^GATC
SexAI 483 ACCTGGT A^CC[AT]GGT
SfcI 229 CTGCAG C^T[GA][CT]AG
SfuI 584 TTCGAA TT^CGAA
SmlI 510 CTCAAG C^T[CT][GA]AG
SspBI 549 TGTACA T^GTACA
SspI 5 AATATT AAT^ATT
Van91I 349 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BmyI 75 GTGCCC G[AGT]GC[ACT]^C
309 GGGCCC
BsiYI 349 CCAGCTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
989 CCAAAAAGAGG
BslI 349 CCAGCTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
989 CCAAAAAGAGG
Bsp1286I 75 GTGCCC G[AGT]GC[ACT]^C
309 GGGCCC
BssKI 355 CCTGG ^CC[ACGT]GG
484 CCTGG
BstF5I 28 GGATG GGATG(2/0)
771 GGATG
BstNI 355 CCTGG CC^[AT]GG
484 CCTGG
EcoRII 355 CCTGG ^CC[AT]GG
484 CCTGG
FokI 28 GGATG GGATG(9/13)
771 GGATG
HaeIII 32 GGCC GG^CC
310 GGCC
MaeII 600 ACGT A^CGT
727 ACGT
MvaI 355 CCTGG CC^[AT]GG
484 CCTGG
MwoI 231 GCAGAGATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
398 GCACAGATTGC
NlaIII 395 CATG CATG^
651 CATG
NlaIV 54 GGAACC GG[ACGT]^[ACGT]CC
309 GGGCCC
ScrFI 355 CCTGG CC^[ACGT]GG
484 CCTGG
SfaNI 772 GATGC GCATC(5/9)
578 GCATC
Tsp45I 818 GTGAC ^GT[GC]AC
1027 GTGAC
TspRI 520 CAGTG CA[GC]TG[ACGT][ACGT]^
767 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AfaI AluI BpmI BsrDI Cfr13I
DdeI HinfI MaeIII MboII MnlI
MseI RsaI Sau96I TaqI TfiI
Tru9I Tsp509I TthHB8I