################################## # NO CUT ENZYMES ( 169 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BpmI BsaAI BsaBI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstF5I BstXI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DpnII DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HgaI HincII HindII HindIII HpaI KpnI KspI MaeI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI NaeI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp45I Tsp509I TspRI Tth111I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AccII 135 CGCG CG^CG AcyI 79 GGCGCC G[GA]^CG[CT]C AfaI 171 GTAC GT^AC AspHI 193 GAGCAC G[AT]GC[AT]^C BanI 79 GGCGCC G^G[CT][GA]CC BanII 96 GGGCTC G[GA]GC[CT]^C BbeI 79 GGCGCC GGCGC^C BbsI 114 GAAGAC GAAGAC(2/6) BbvI 33 GCTGC GCAGC(8/12) BlpI 98 GCTCAGC GC^T[ACGT]AGC Bpu1102I 98 GCTCAGC GC^T[ACGT]AGC BpuAI 114 GAAGAC GAAGAC(2/6) BsaHI 79 GGCGCC G[GA]^CG[CT]C BsaI 201 GAGACC GGTCTC(1/5) BsiHKAI 193 GAGCAC G[AT]GC[AT]^C BsmAI 201 GAGAC GTCTC(1/5) BsmFI 45 GTCCC GGGAC(10/14) BsrI 310 ACTGG ACTGG(1/-1) BssSI 196 CACGAG CACGAG(-5/-1) BstEII 265 GGTAACC G^GT[ACGT]ACC BstNI 254 CCTGG CC^[AT]GG BstPI 265 GGTAACC G^GT[ACGT]ACC BstUI 135 CGCG CG^CG CelII 98 GCTCAGC GC^T[ACGT]AGC DdeI 99 CTCAG C^T[ACGT]AG EarI 91 GAAGAG CTCTTC(1/4) EcoO65I 265 GGTAACC G^GT[ACGT]ACC EcoRII 254 CCTGG ^CC[AT]GG EcoT14I 84 CCATGG C^C[AT][AT]GG FauI 47 CCCGC CCCGC(4/6) HaeII 79 GGCGCC [GA]GCGC^[CT] HaeIII 65 GGCC GG^CC HapII 143 CCGG C^CGG Hin1I 79 GGCGCC G[GA]^CG[CT]C HpaII 143 CCGG C^CGG HphI 122 TCACC GGTGA(8/7) KasI 79 GGCGCC G^GCGCC Ksp632I 91 GAAGAG CTCTTC(1/4) MaeIII 266 GTAAC ^GT[ACGT]AC MspA1I 55 CAGCTG C[AC]G^C[GT]G MspI 143 CCGG C^CGG MvaI 254 CCTGG CC^[AT]GG MvnI 135 CGCG CG^CG NarI 79 GGCGCC GG^CGCC NcoI 84 CCATGG C^CATGG PleI 117 GACTC GAGTC(4/5) PvuII 55 CAGCTG CAG^CTG RsaI 171 GTAC GT^AC SfaNI 233 GCATC GCATC(5/9) SfoI 79 GGCGCC GGC^GCC SmaI 142 CCCGGG CCC^GGG SphI 158 GCATGC GCATG^C StyI 84 CCATGG C^C[AT][AT]GG TaqI 73 TCGA T^CGA TfiI 297 GAATC G^A[AT]TC TseI 33 GCTGC G^C[AT]GC TthHB8I 73 TCGA T^CGA XmaCI 142 CCCGGG C^CCGGG XmaI 142 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AluI 56 AGCT AG^CT 102 AGCT AvaI 142 CCCGGG C^[CT]CG[GA]G 205 CCCGAG AvaII 44 GGTCC G^G[AT]CC 239 GGACC BcnI 142 CCCGG CC^[GC]GG 143 CCGGG BmyI 96 GGGCTC G[AGT]GC[ACT]^C 193 GAGCAC BsoBI 142 CCCGGG C^[CT]CG[GA]G 205 CCCGAG Bsp1286I 96 GGGCTC G[AGT]GC[ACT]^C 193 GAGCAC Cac8I 103 GCTCGC GC[ACGT]^[ACGT]GC 158 GCATGC CfoI 80 GCGC GCG^C 136 GCGC Cfr13I 44 GGTCC G^G[ACGT]CC 239 GGACC DsaI 84 CCATGG C^C[GA][CT]GG 186 CCACGG Fnu4HI 33 GCTGC GC^[ACGT]GC 63 GCGGC HhaI 80 GCGC GCG^C 136 GCGC HinP1I 80 GCGC G^CGC 136 GCGC HinfI 117 GACTC G^A[ACGT]TC 297 GAATC ItaI 33 GCTGC GC^[ACGT]GC 63 GCGGC NciI 142 CCCGG CC^[GC]GG 143 CCGGG NlaIV 44 GGTCCC GG[ACGT]^[ACGT]CC 79 GGCGCC NspI 107 GCATGT [GA]CATG^[CT] 158 GCATGC Sau96I 44 GGTCC G^G[ACGT]CC 239 GGACC ######################################### # 3 OR MORE CUT ENZYMES ( 10 enzymes) # ######################################### AciI BsaJI BsiYI BslI BssKI MboII MnlI MwoI NlaIII ScrFI