################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BcgI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BspEI BssHII BssSI BstBI BstEII BstPI Bsu36I CelII CfoI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV FseI FspI HaeII HgaI HhaI Hin1I HinP1I KasI KpnI KspI MaeII MamI MluI MroI MspA1I NarI NciI NdeI NheI NotI NruI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI StuI SwaI Tth111I Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AccI 1503 GTATAC GT^[AC][GT]AC AccII 1386 CGCG CG^CG AflIII 962 ACATGT A^C[GA][CT]GT Asp700I 937 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 1232 CTCGAG C^[CT]CG[GA]G BciVI 79 GGATAC GTATCC(6/5) BclI 497 TGATCA T^GATCA BglII 1443 AGATCT A^GATCT BpmI 772 CTGGAG CTGGAG(16/14) BsgI 1129 CTGCAC GTGCAG(16/14) BsmAI 1177 GAGAC GTCTC(1/5) BsmBI 1177 GAGACG CGTCTC(1/5) BsmFI 675 GGGAC GGGAC(10/14) BsmI 1003 GAATGC GAATGC(1/-1) BsoBI 1232 CTCGAG C^[CT]CG[GA]G Bsp1407I 1247 TGTACA T^GTACA BspDI 1666 ATCGAT AT^CGAT BspHI 1835 TCATGA T^CATGA BspLU11I 962 ACATGT A^CATGT BsrBI 1189 GAGCGG CCGCTC(-3/-3) BsrDI 74 GCAATG GCAATG(2/0) BsrFI 1037 GCCGGC [GA]^CCGG[CT] BsrGI 1247 TGTACA T^GTACA Bst1107I 1503 GTATAC GTA^TAC BstAPI 1747 GCAAAATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 1386 CGCG CG^CG BstXI 1757 CCATCTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1443 AGATCT [GA]^GATC[CT] BstZ17I 1503 GTATAC GTA^TAC Cfr10I 1037 GCCGGC [GA]^CCGG[CT] ClaI 1666 ATCGAT AT^CGAT DrdI 1555 GACATTATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 234 CCATGG C^C[GA][CT]GG EcoNI 645 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 234 CCATGG C^C[AT][AT]GG EcoT22I 1005 ATGCAT ATGCA^T FauI 975 GCGGG CCCGC(4/6) FbaI 497 TGATCA T^GATCA HapII 1038 CCGG C^CGG HpaI 1225 GTTAAC GTT^AAC HpaII 1038 CCGG C^CGG MfeI 521 CAATTG C^AATTG MflI 1443 AGATCT [GA]^GATC[CT] MspI 1038 CCGG C^CGG MunI 521 CAATTG C^AATTG MvnI 1386 CGCG CG^CG NaeI 1037 GCCGGC GCC^GGC NcoI 234 CCATGG C^CATGG NgoMIV 1037 GCCGGC G^CCGGC NsiI 1005 ATGCAT ATGCA^T PaeR7I 1232 CTCGAG C^TCGAG PstI 1012 CTGCAG CTGCA^G RcaI 1835 TCATGA T^CATGA ScaI 868 AGTACT AGT^ACT SphI 1007 GCATGC GCATG^C SspBI 1247 TGTACA T^GTACA StyI 234 CCATGG C^C[AT][AT]GG TfiI 1404 GAATC G^A[AT]TC XcmI 1079 CCAAGTTGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1232 CTCGAG C^TCGAG XhoII 1443 AGATCT [GA]^GATC[CT] XmnI 937 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AspHI 37 GAGCTC G[AT]GC[AT]^C 802 GAGCTC BalI 2 TGGCCA TGG^CCA 1536 TGGCCA BanI 337 GGCACC G^G[CT][GA]CC 614 GGTGCC BbsI 1707 GAAGAC GAAGAC(2/6) 1804 GAAGAC BfaI 329 CTAG C^TAG 1616 CTAG BpuAI 1707 GAAGAC GAAGAC(2/6) 1804 GAAGAC BsiHKAI 37 GAGCTC G[AT]GC[AT]^C 802 GAGCTC BspMI 902 ACCTGC ACCTGC(4/8) 912 GCAGGT DraII 641 AGGACCT [GA]G^G[ACGT]CC[CT] 1625 AGGACCC EaeI 2 TGGCCA [CT]^GGCC[GA] 1536 TGGCCA EcoO109I 641 AGGACCT [GA]G^G[ACGT]CC[CT] 1625 AGGACCC MaeI 329 CTAG C^TAG 1616 CTAG MluNI 2 TGGCCA TGG^CCA 1536 TGGCCA MscI 2 TGGCCA TGG^CCA 1536 TGGCCA PleI 883 GACTC GAGTC(4/5) 1235 GAGTC PpuMI 641 AGGACCT [GA]G^G[AT]CC[CT] 1625 AGGACCC SacI 37 GAGCTC GAGCT^C 802 GAGCTC SapI 799 GAAGAGC GCTCTTC(1/4) 1186 GAAGAGC SexAI 1680 ACCAGGT A^CC[AT]GGT 1808 ACCTGGT SfcI 176 CTACAG C^T[GA][CT]AG 1012 CTGCAG Tsp45I 1048 GTCAC ^GT[GC]AC 1253 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BanII BbvCI BbvI BmyI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HincII HindII HindIII HinfI HphI ItaI Ksp632I MaeIII MboI MboII MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV NspI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI TaqI Tru9I TseI Tsp509I TspRI TthHB8I